Article
Engineering, Biomedical
Shadab Abadpour, Essi M. Niemi, Linnea Strid Orrhult, Carolin Hermanns, Rick de Vries, Liebert Parreiras Nogueira, Havard Jostein Haugen, Dag Josefsen, Stefan Krauss, Paul Gatenholm, Aart van Apeldoorn, Hanne Scholz
Summary: This study reports the manufacturing of a 3D bioprinted double-layered scaffold using alginate/nanofibrillated cellulose (NFC) bioink to combine islets and adipose-derived stromal cells (ASCs) for beta cell replacement therapy. The results demonstrate that ASCs improve islet viability, glucose sensing, insulin secretion, and reduce pro-inflammatory cytokine secretion. Diabetic mice transplanted with islet-ASC scaffolds achieve normoglycemia and show elevated levels of C-peptide.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Polymer Science
Izar Gorronogoitia, Uzuri Urtaza, Ana Zubiarrain-Laserna, Ana Alonso-Varona, Ane Miren Zaldua
Summary: Three-dimensional bioprinting combined with natural hydrogels is a promising technology for the treatment of various pathologies and tissue regeneration. This study focuses on the printability and physicochemical properties of alginate-based hydrogels and scaffolds. The results show that the microstructure of alginate has a significant impact on its printability and mechanical properties, emphasizing the importance of controlling the viscoelastic and mechanical properties for obtaining high-resolution and precise structures.
Article
Chemistry, Multidisciplinary
Chongjian Gao, Lan Tang, Huawei Qu, Mingming Wu, Tian Zhou, Chunyi Wen, Pinpin Wang, Nan Xu, Changshun Ruan
Summary: Introduced a strategy of pre-crosslinking and post-crosslinking improvement for methacrylated alginate (AlgMA) bioinks in extrusion bioprinting through the introduction of a small-molecule polycationic crosslinker, methacrylated e-polylysine (e-PLMA). The e-PLMA significantly reinforced the operability and stability of AlgMA bioinks and improved the viability and vitality of cells in printed constructs. This strategy shows great potential in improving Alg-based extrusion bioprinting and expanding its biomedical applications.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Johannes Windisch, Olena Reinhardt, Sarah Duin, Kathleen Schuetz, Nuria Juliana Novoa Rodriguez, Suihong Liu, Anja Lode, Michael Gelinsky
Summary: Bioprinting, a key technology for future space missions, is being established on the International Space Station (ISS). Researchers have investigated the storability of bioinks, aiming to produce bioinks on Earth and transport them to the ISS. The study shows that bioinks containing microalgae or human cells can be stored at 4 degrees C for four weeks without losing printability, but the viability of human cells decreases during storage. This research provides a promising starting point for future studies on storing bioinks and improving complex printing processes.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Xavier Barcelo, Kian F. Eichholz, Orquidea Garcia, Daniel J. Kelly
Summary: The study investigated the effect of alginate oxidation on the degradation rate of bioinks, resulting in a range of degradable bioinks with the degradation rate highly dependent on the content of partially oxidized alginate. Despite high mass loss, all bioink blends except the 0:100 group maintained the initially printed geometry throughout a 4 week in vitro culture period and supported robust chondrogenic differentiation of mesenchymal stem/stromal cells.
Article
Chemistry, Physical
Vera Guduric, Niall Belton, Richard Frank Richter, Anne Bernhardt, Janina Spangenberg, Chengtie Wu, Anja Lode, Michael Gelinsky
Summary: This study synthesized different types of composites containing zinc in mesoporous bioactive glasses (MBG) known for their osteogenic and antimicrobial properties, suitable for bone tissue engineering bioprinting applications, and demonstrated the adaptability of these composites to bone cells.
Article
Cell & Tissue Engineering
Mar Bonany, Laura del-Mazo-Barbara, Montserrat Espanol, Maria-Pau Ginebra
Summary: This study explored the incorporation of microspheres into alginate-based bioinks as a solution to the lack of cell adhesion in alginate. The results showed that the addition of microspheres improved the rheological properties of the ink, promoted cell proliferation, and positively influenced osteogenic cell differentiation. The type of microsphere and the ability of cells to migrate towards them were found to affect cell differentiation, which was also dependent on the stiffness of the bioink.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Cell & Tissue Engineering
Tomas Gonzalez-Fernandez, Alejandro J. Tenorio, Kevin T. Campbell, Eduardo A. Silva, J. Kent Leach
Summary: This study evaluated four commonly used alginate-based bioinks for 3D bioprinting of bone tissues, highlighting the importance of bioink properties in modulating cell behavior and the biofabrication of clinically relevant bone tissues. The study found that the physical properties of bioinks significantly affected the biological performance of encapsulated mesenchymal stromal cells and their osteogenic potential. Additionally, the study identified alginate-CaCl2 as the most effective bioink for promoting osteogenic differentiation and partial mineralization of cell-laden bone constructs.
TISSUE ENGINEERING PART A
(2021)
Article
Engineering, Biomedical
Giuseppe Guagliano, Cristina Volpini, Jacopo Camilletti, Francesca Donnaloja, Francesco Briatico-Vangosa, Livia Visai, Paola Petrini
Summary: Bioprinting is a key technique for fabricating cell-laden constructs with controlled geometry. We implemented an unconventional strategy by optimizing the design of structures to promote high oxygenation, mimicking the characteristics of hepatic tissue. Through computational methods and a combination of a priori and a posteriori analyses, we successfully printed constructs using an internally crosslinked bioink and cultured them for up to 12 days, demonstrating the suitability of Hep3Gel for mid/long-term cultures.
Article
Chemistry, Medicinal
Qiqi Gao, Byoung-Soo Kim, Ge Gao
Summary: Alginate hydrogel as a bioink for 3D bioprinting has advantages of low cost, good biocompatibility, and rapid ionic gelation, but faces limitations such as lack of cell adhesive moieties, erratic biodegradability, and poor printability. Various advanced material formulations and biofabrication strategies have been developed to overcome these drawbacks and enhance the applications of alginate-based bioink in tissue engineering.
Article
Chemistry, Multidisciplinary
Hui Zhu, Mahshid Monavari, Kai Zheng, Thomas Distler, Liliang Ouyang, Susanne Heid, Zhaorui Jin, Jiankang He, Dichen Li, Aldo R. Boccaccini
Summary: Bioprinting has made significant progress in recent years in the fabrication of complex bionic tissues. However, developing cell-laden bioinks with superior physiochemical properties and bio-functionality remains challenging. In this study, a multifunctional nanocomposite bioink was developed using amine-functionalized copper-doped mesoporous bioactive glass nanoparticles and a hydrogel formulation based on dynamic covalent chemistry. This bioink showed favorable rheological properties, improved shape fidelity, and structural stability for extrusion-based bioprinting. The introduction of aminated particles and the reversible dynamic microenvironment enabled rapid spreading and high survival of embedded cells. The bioprinted scaffolds promoted osteogenic differentiation and angiogenesis of cells without additional growth factors in vitro. These nanocomposite bioinks have great potential for bioprinting complex 3D environments in bone tissue engineering.
Article
Polymer Science
Emine Karakaya, Faina Bider, Andreas Frank, Joerg Tessmar, Lisa Schoebel, Leonard Forster, Stefan Schruefer, Hans-Werner Schmidt, Dirk Wolfram Schubert, Andreas Blaeser, Aldo R. Boccaccini, Rainer Detsch
Summary: In the context of bioprinting, targeted printing of cells at specific positions is a novel approach that offers new possibilities for life science engineering. However, achieving high cell survival rates on gel substrates remains a challenge. This study evaluates the use of alginate-dialdehyde-polyethylene glycol (ADA-PEG) inks for drop on demand (DoD) printing, analyzing their biochemical properties, printing process, cell distribution, and shear forces. The findings highlight the importance of PEG modifications and chain lengths in achieving stable hydrogels and biocompatibility, as well as the need to determine the optimal cell concentration for targeted cell printing.
Review
Medicine, Research & Experimental
Qian Hua Phua, Hua Alexander Han, Boon-Seng Soh
Summary: Significant progress has been made in skin tissue engineering, with the main obstacle to successful engraftment being the incorporation of functional vasculature to provide nutrients and oxygen supply to embedded cells. New technologies such as 3D bioprinting, nano-fabrication, and micro-patterning using hydrogel-based scaffolds are rapidly developing to address the limitations of traditional tissue engineering.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Engineering, Biomedical
Qi Li, Bin Zhang, Qian Xue, Chunxiao Zhao, Yichen Luo, Hongzhao Zhou, Liang Ma, Huayong Yang, Dapeng Bai
Summary: Understanding the relationship between printing temperature and materials' printability is crucial for improving the accuracy of 3D bioprinting technology. The study establishes a thermal model incorporating different printing temperatures to predict printing outcomes and optimize the extrusion-based printability of bioinks.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Article
Biochemistry & Molecular Biology
Sarah Duin, Shreya Bhandarkar, Susann Lehmann, Elisabeth Kemter, Eckhard Wolf, Michael Gelinsky, Barbara Ludwig, Anja Lode
Summary: The transplantation of pancreatic islets can prevent complications in diabetes patients. 3D bioprinting can encapsulate islets and improve the success rate of transplantation. This study successfully bioprinted neonatal porcine islet-like cell clusters and demonstrated their functionality in vitro.
