Article
Polymer Science
Maria Herrero-Herrero, Jose Antonio Gomez-Tejedor, Ana Valles-Lluch
Summary: Tailored membranes with a fiber diameter of 1.8 μm were electrospun from PLGA and PCLGA solutions by adjusting electrospinning and polymer solution parameters. PCLGA, with its amorphous morphology, required the addition of a salt to increase conductivity for successful electrospinning, presenting notable difficulties due to its structure. Interestingly, PCLGA, with a higher glycolic acid molar fraction, could be successfully electrospun, a feat not previously reported.
Article
Chemistry, Multidisciplinary
Yiye Lu, Geoffrey W. Coates
Summary: Poly(lactic-co-glycolic acid) (PLGA) is widely used in various biomedical applications due to its biodegradability and biocompatibility. This study successfully developed a method to synthesize syndioenriched, alternating PLGA from racemic methyl-glycolide (rac-MeG), and confirmed the high degree of alternation of the comonomer sequence and moderate syndiotacticity within the polymer backbone stereoconfiguration through NMR investigations.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Yiye Lu, Geoffrey W. Coates
Summary: Poly(lactic-co-glycolic acid) (PLGA) is a biodegradable and biocompatible polymer used in biomedical applications. This study presents a method for synthesizing syndioenriched, alternating PLGA from racemic methyl-glycolide with the use of an optimized racemic aluminum catalyst. The resulting material has a high degree of alternation in copolymer sequence and moderate syndiotacticity, and is amorphous, making it suitable for drug complexation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Sankaran Vijayalakshmi, Aruliah Rajasekar, Vishnu Priya Veeraraghavan, Alaa Yousef Ghidan, Tawfiq M. Al Antary, Venkatachalam Karthikkumar, Lakshmi Priya Malarveni Damodaran, Vinayagam Ramachandran, Ernest David
Summary: The study revealed that POL-PLGA-NPs effectively induced oxidative stress, leading to cell damage and apoptosis in KB cells.
PROCESS BIOCHEMISTRY
(2021)
Article
Polymer Science
Hong Wang, Yanwei Zhang, Yaru Liu, Yi Ren, Jianhong Wang, Baolong Niu, Wenfeng Li
Summary: This paper focuses on the development of targeted drug delivery systems for cancer therapy. HA-SS-PLGA copolymer was used to form reduction-sensitive amphiphilic micelles, which actively targeted tumor cells and inhibited cancer cell proliferation. This research provides a promising drug carrier for cancer therapy.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Pharmacology & Pharmacy
Anita Kirti Ghosh, Rubina Thapa, Harsh Nilesh Hariani, Michael Volyanyuk, Sean David Ogle, Karoline Anne Orloff, Samatha Ankireddy, Karen Lai, Agne Ziniauskaite, Evan Benjamin Stubbs Jr, Giedrius Kalesnykas, Jenni Johanna Hakkarainen, Kelly Ann Langert, Simon Kaja
Summary: This study demonstrated the pharmacological efficacy of xanthohumol in preclinical models for dry eye disease by promoting the transcription of phase II antioxidant enzymes. Xanthohumol-encapsulating PLGA nanoparticles showed cytoprotective effects against oxidative stress in vitro and reduced ocular surface damage in vivo, suggesting their potential as future therapeutic options for dry eye disease.
Article
Polymer Science
Jie Yan, Paula Facal Marina, Anton Blencowe
Summary: Poly(ethylene glycol)-b-poly(lactic-co-glycolic acid) (PEG-PLGA) block copolymers and their thermoresponsive hydrogels have been widely studied for sustained drug delivery. The microstructure of these copolymers is highly sensitive to the reaction conditions, such as temperature and catalyst loading, which influence the occurrence and extent of side reactions during ring-opening polymerization. Our detailed study revealed that increasing the catalyst loading had negligible effects on side reactions but surprisingly increased the polymerization rate of lactide relative to glycolide. These findings provide valuable insights for the improved synthesis of PLGA-PEG-PLGA copolymers and their hydrogels.
Article
Chemistry, Multidisciplinary
Qin Chang, Dong Jing, Xie Beibei, Wang Hongkun, Zhang Na, Zhao Chunhua, Qiao Congde, Liu Mingxia, Yang Xiaodeng, Li Tianduo
Summary: Poly(lactic-co-glycolic acid)(PLGA) with different molecular weights and LA/GA molar ratio was prepared and characterized in this study. The results demonstrated that PLGA(3.2) film with an LA/GA molar ratio of 52/48 is a promising curcumin carrier.
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2023)
Article
Agriculture, Dairy & Animal Science
B. S. Ananda Kumar, Sivasankar Panickan, Suresh Bindu, Vimal Kumar, Saravanan Ramakrishnan, Sonal Saxena, Sameer Shrivastava, Satyabrata Dandapat
Summary: An immunization experiment using PLGA nanoparticles encapsulating inactivated NDV vaccine was conducted in specific pathogen-free chickens. The results showed that the nano-NDV vaccine induced high levels of antibodies and cell-mediated immune responses, providing 100% protection against NDV challenge. This study provides insights for developing PLGA nanoparticle-based vaccines against avian diseases.
Article
Pharmacology & Pharmacy
Seung-No Hong, Minjae Kim, Jin-A Park, Minji Kang, Hyunkyung Cha, Sohyun Park, Joon Kon Kim, Jinyoung Pac, Yuju Seo, Sungwhan Kim, Minju Kim, Dae Woo Kim, Yan Lee
Summary: This study designed dexamethasone-loaded poly(lactic-co-glycolic acid) microparticles with bone-specific binding affinity for the treatment of sinus surgery-induced osteitis in chronic rhinosinusitis (CRS). The microparticles released the encapsulated dexamethasone in a sustained manner on the exposed bone after the surgical wound in the nasal cavity. The results showed that the microparticles had higher binding affinity and inhibited the formation of undesirable new woven bone. Therefore, this localized corticoid delivery method has the potential to treat CRS-related osteitis with minimal side effects.
Review
Materials Science, Biomaterials
Jagan Mohan Dodda, Tomas Remis, Sadiku Rotimi, Yi-Cheun Yeh
Summary: This article provides an overview of PLGA-based conjugates in cancer research, focusing on their roles as targeted drug carriers and sustained drug controllers. The preparation and drug encapsulation of PLGA nanoparticles and FA-PEG-PLGA conjugates are discussed, with emphasis on the associated chemistries and biocompatibility. This review offers a deeper understanding of PLGA-conjugated materials for successful material design and biomedical applications.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biophysics
Ibrahima Youm, Matthew B. West, Xiangping Huang, Wei Li, Richard D. Kopke
Summary: The effects of sucrose on the physical stability, cellular entry pathways, and functional efficacy of PLGA-NPs were investigated in this study. The results showed that the addition of 10% sucrose significantly improved the physical stability and cellular uptake of HEI-101-NPs.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Biotechnology & Applied Microbiology
Nasrullah Jan, Asadullah Madni, Safiullah Khan, Hassan Shah, Faizan Akram, Arshad Khan, Derya Ertas, Mohammad F. Bostanudin, Christopher H. Contag, Nureddin Ashammakhi, Yavuz Nuri Ertas
Summary: This article discusses the preparation methods and applications of cell membrane-coated PLGA nanoparticles, as well as their role in cancer therapy, inflammation management, cardiovascular disease treatment, and infection control. It also highlights the challenges and future research directions of cell membrane-camouflaged nanoparticles.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Rong Wang, Xiaojie Sun, Lanlan Chen, Wenbin Liang
Summary: In this study, biodegradable blends of PGA and PBAT with in situ compatibilization using MDI were prepared. The addition of PBAT was found to increase the complex viscosity and storage modulus of the blends, leading to improved compatibility and impact strength. The morphology of the dispersed phase evolved from spherical structure to in situ microfiber, enhancing interfacial adhesion and improving toughness.
Article
Materials Science, Multidisciplinary
Akhand Pratap Singh, Om Prakash, Sunil Kumar, Aparna Shukla, Pralay Maiti
Summary: The study demonstrates the suitability of poly(lactic acid-co-glycolic acid) (PLGA) as a sustained drug delivery vehicle for melanoma treatment. PLGA nanoparticles (NPs) and PLGA film encapsulated with paclitaxel (PTX) show greater efficacy in inhibiting melanoma cell proliferation and reducing tumor volume, compared to pure drug and control group. The sustained drug delivery systems also result in minimal damage to vital organs and fewer side effects.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Amanda Cano, Miren Ettcheto, Mireia Bernuz, Raquel Puerta, Ester Esteban de Antonio, Elena Sanchez-Lopez, Eliana B. Souto, Antonio Camins, Merce Marti, Maria Isabel Pividori, Merce Boada, Agustin Ruiz
Summary: Extracellular vesicles are secreted by cells and play important roles in intercellular communication, immune responses, human reproduction, and synaptic plasticity. They have potential as biomarkers for diseases due to their biological relevance and easy accessibility. They can also be used as mirrors of brain diseases as they can cross the blood-brain barrier. This review explores their biogenesis, functions, and potential in clinical practice, as well as their role in major human brain diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Bianca P. Genesi, Raquel de Melo Barbosa, Patricia Severino, Andrea C. D. Rodas, Cristiana M. P. Yoshida, Monica B. Mathor, Patricia S. Lopes, Cesar Viseras, Eliana B. Souto, Classius Ferreira da Silva
Summary: Chitosan films containing aloe vera and copaiba oil were developed and evaluated for their antimicrobial activity, cytotoxicity, and in vivo healing potential. The results showed that these films had excellent properties in promoting wound healing.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Gerard Esteruelas, Eliana B. Souto, Marta Espina, Maria Luisa Garcia, Marta Switalska, Joanna Wietrzyk, Anna Gliszczynska, Elena Sanchez-Lopez
Summary: Cancer is a leading cause of death worldwide, but there is still a need for an effective treatment with minimal adverse effects. Diclofenac, a non-steroidal anti-inflammatory drug, has shown promise as an antitumoral agent, but its prolonged use can lead to adverse effects. In this study, biodegradable polymeric nanoparticles were used to deliver diclofenac in a controlled manner, resulting in prolonged release and demonstrating antitumoral effects without cytotoxicity to non-tumoral cells.
Article
Biochemistry & Molecular Biology
Helen-Lissette Alvarado, David Limon, Ana-Cristina Calpena-Campmany, Mireia Mallandrich, Laura Rodriguez-Cid, Nuria Aliaga-Alcalde, Arantzazu Gonzalez-Campo, Lluisa Perez-Garcia
Summary: This study evaluated the preventive effects of curcumin and its derivatives on the irritant effects of topically applied xylol and their permeation ability through human skin. An HPLC method was validated for simultaneous determination of the curcuminoids in the samples from the ex vivo permeation studies and drug extraction from the skin. The results show that these three curcuminoids are suitable for anti-inflammatory formulations for dermal applications, and they can be properly quantified using HPLC-UV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Ruth M. Galindo-Camacho, Isabel Haro, Maria J. Gomara, Marta Espina, Joel Fonseca, Carlos Martins-Gomes, Antoni Camins, Amelia M. Silva, Maria L. Garcia, Eliana B. Souto
Summary: The biocompatibility of cell penetrating peptides-functionalized Licochalcone-A-loaded PLGA nanoparticles was evaluated in Caco-2 cell lines, demonstrating non-cytotoxicity, while the anti-inflammatory activity of the nanoparticles was tested in RAW 264.7 cell lines. Freeze-drying with different cryoprotectants was performed to prevent particle aggregation and physical stress. An in-situ forming gel based on poloxamer 407 containing Licochalcone-A-loaded PLGA nanoparticles functionalized with B6 and Tet-1 was developed for ocular administration, providing viscoelasticity, suitable mechanical properties, and mucoadhesive performance.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Zielinska, Raquel da Ana, Joel Fonseca, Milena Szalata, Karolina Wielgus, Faezeh Fathi, M. Beatriz P. P. Oliveira, Rafal Staszewski, Jacek Karczewski, Eliana B. Souto
Summary: Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) were used to encapsulate CBD, and their physicochemical characteristics were investigated. The results showed that CBD-loaded SLN had a mean particle size of 217.2 +/- 6.5 nm, PDI of 0.273 +/- 0.023, and EE of about 74%, while CBD-loaded NLC had Z-Ave of 158.3 +/- 6.6 nm, PDI of 0.325 +/- 0.016, and EE of about 70%. Rheological analysis indicated that both lipid nanoparticle formulations exhibited more elasticity than viscosity. SEM study confirmed the morphology and shape of the nanoparticles.
Article
Forestry
David Franco Frazao, Jose Carlos Goncalves, Amelia M. Silva, Fernanda Delgado
Summary: This study evaluated the productivity of labdanum resin, seeds, and biomass in a 5-year-old shrubland, finding that annual harvest maximizes labdanum resin and photosynthetic biomass productivity, while biennial harvest maximizes seed and lignified biomass productivity.
Review
Green & Sustainable Science & Technology
Patricia Gatinho, Catia Salvador, Amelia M. Silva, Ana Teresa Caldeira
Summary: Pristine environments like caves offer rich microbial diversity, with microorganisms developing unique survival skills such as biosynthesis of bioactive secondary metabolites. Studying microbial strains from cave surfaces is important to explore the biotechnological potential of these organisms, leading to the discovery of new products with various bioactivities. Recent advancements in genomics and bioinformatics have made the exploration of bacterial secondary metabolites more promising, highlighting the importance of these habitats as a potential source of bioactive compounds for sustainable biotechnological applications.
Article
Pharmacology & Pharmacy
Paola Bustos-Salgado, Berenice Andrade-Carrera, Valeri Dominguez-Villegas, Veronique Noe, Mireia Mallandrich, Helena Colom, Ana Calpena-Campmany, Maria Luisa Garduno-Ramirez
Summary: This study evaluated the anticancer potential of the natural flavanone compound extracted from Eysenhardtia platycarpa and its derivatives through in silico analysis and cytotoxicity assays. The results showed that these compounds inhibited the growth of tumor cells, with 1b exhibiting the most significant effect. Moreover, the study demonstrated the ability of PLGA nanoparticles to penetrate the skin and prolong the duration of action. Overall, flavanones could be a promising option for future anticancer topical adjuvant treatment.
Article
Polymer Science
Noelia Perez-Gonzalez, Maria J. Rodriguez-Lagunas, Ana C. Calpena-Campmany, Nuria Bozal-de Febrer, Lyda Halbaut-Bellowa, Mireia Mallandrich, Beatriz Clares-Naveros
Summary: Fungal keratitis is a global cause of corneal blindness. Current treatment involves the use of antibiotics, with Natamycin being commonly used; however, alternative therapies are necessary due to the difficulty in treating fungal keratitis. In situ gelling formulations, which combine the advantages of eye drops and ointments, show promise as an alternative. This study aimed to develop and characterize three formulations containing 0.5% CSP: CSP-O1, CSP-O2, and CSP-O3. The results suggest that these formulations could be potential candidates for the treatment of fungal keratitis.
Article
Biochemistry & Molecular Biology
Ana Abraao, Carlos Martins-Gomes, Raul Dominguez-Perles, Ana Barros, Amelia M. Silva
Summary: This study aimed to determine the phenolic profile and health-promoting activities of hydroethanolic extracts obtained from Prunus lusitanica fruits harvested from three different locations. The results showed that the extracts contained high levels of phenolic compounds and exhibited high antibacterial activity and neuroprotective potential, as well as moderate anti-aging and anti-diabetic effects. Therefore, the bioactive molecules in Prunus lusitanica fruits deserve further exploration for the development of new drugs in the pharmaceutical and cosmetic industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Tatiana N. Pashirova, Andrey V. Nemtarev, Eliana B. Souto, Vladimir F. Mironov
Summary: This review provides a systematic survey of studies on mitochondria-targeted nanosystems containing triphenylphosphonium vector groups, summarizing the design principles and preparation methods for these nanosystems and analyzing the relationship between key parameters and biological action.
RUSSIAN CHEMICAL REVIEWS
(2023)
Article
Pharmacology & Pharmacy
Marcelle Silva-Abreu, Lilian Sosa, Lupe Carolina Espinoza, Maria-Jose Fabrega, Maria J. Rodriguez-Lagunas, Mireia Mallandrich, Ana Cristina Calpena, Maria Luisa Garduno-Ramirez, Maria Rincon
Summary: Apremilast (APM) is a novel drug for the treatment of psoriasis and psoriatic arthritis. This study aims to develop APM gels as a new pharmaceutical formulation for topical psoriasis treatment. The APM gel formulations showed good physicochemical characteristics and sustained release. The gels treated the psoriasis mouse model and reduced the expression of proinflammatory cytokines.
Article
Chemistry, Physical
Gabrielle B. Novais, Mariana A. Dias, Adilson A. M. Santana, Thigna C. Batista, Maria N. Marques, Carlisson R. Melo, Ricardo L. C. Albuquerque Junior, Margarete Z. Gomes, Patricia Severino, Eliana B. Souto, Juliana C. Cardoso
Summary: This work focuses on the functionalization of SWCNTs and MWCNTs with two selected isoflavones and compares their physicochemical properties. Acidic treatment improved the dispersibility of both types of nanotubes, but SWCNTs still remained aggregated. However, MWCNTs showed increased dispersibility after acidic treatment. The functionalization with isoflavones was confirmed by improved dispersibility of the nanotubes.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Review
Multidisciplinary Sciences
Isabella S. A. Peres, Kiara A. O. Conceicao, Larissa A. F. Silva, Nadia G. Khouri, Cristiana M. P. Yoshida, Viktor O. C. Concha, Massimo Lucarini, Alessandra Durazzo, Antonello Santini, Eliana B. Souto, Patricia Severino
Summary: This article discusses the nutraceutical and pharmaceutical applications of Dragon's Blood, focusing on its antioxidant properties. Through literature research analysis, the current status and future development directions of this topic are explored. The study reveals that Dragon's Blood extracts possess various activities such as anti-inflammatory, wound healing, antidiarrheal, anticancer, antirheumatic, antiseptic, and antioxidant effects, which provide novel perspectives for its use in the food and pharmaceutical industries.
RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI
(2023)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)