Article
Pharmacology & Pharmacy
E. de Pablo, P. O'Connell, R. Fernandez-Garcia, S. Marchand, A. Chauzy, F. Tewes, M. A. Dea-Ayuela, D. Kumar, F. Bolas, M. P. Ballesteros, J. J. Torrado, A. M. Healy, D. R. Serrano
Summary: A carbohydrate-based AmB dry powder inhaler (DPI) formulation was developed to address the lack of effective antifungal pulmonary treatments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Mani Ordoubadi, Kimberly B. Shepard, Hui Wang, Zheng Wang, Amanda M. Pluntze, Joseph P. Churchman, Reinhard Vehring
Summary: Carrier-free spray-dried dispersions for pulmonary delivery often require the addition of excipients such as L-leucine to improve efficacy. However, the presence of leucine fibers during stability has been observed. In this study, the origin of these fibers was determined to be the presence of amorphous or less stable crystalline polymorphs of leucine, but they did not affect the stability or aerosol performance of the powders.
Article
Pharmacology & Pharmacy
Diana A. Fernandes, Eunice Costa, Paula Leandro, M. Luisa Corvo
Summary: This study presents an integrated methodology for the rapid selection of the optimal dry powder inhaler excipient system to develop a spray dried formulation of inhalable enzymes. By screening different sugars and amino acids, excipients that stabilize the quaternary structure of enzymes were selected. After spray drying, the resulting powders exhibited good aerodynamic performance.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Microbiology
Xuerui Bao, Mona Bove, Tom Coenye
Summary: The high tolerance of Pseudomonas aeruginosa biofilms in respiratory tract infections in cystic fibrosis contributes to the failure of antibiotic therapy. This study identified carbon sources that could enhance the inhibiting and/or eradicating activity of tobramycin, ciprofloxacin, and ceftazidime against P. aeruginosa PAO1 biofilms. The mechanisms underlying the enhanced biofilm eradicating activity were strain-dependent.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Pharmacology & Pharmacy
Petra Party, Csilla Bartos, Arpad Farkas, Piroska Szabo-Revesz, Rita Ambrus
Summary: The study focused on developing inhalable nano-in-micro dry powder inhalers containing meloxicam (MX) and additives, targeting the respiratory zone for improved therapeutic effect. By increasing the surface area and amorphization, the dissolution and diffusion of the drug were enhanced to achieve higher drug concentration in the deeper airways, showing suitable aerodynamical properties for treating local lung diseases.
Article
Microbiology
Ayesha Z. Beg, Faraz Rashid, Absar Talat, Mohd Azam Haseen, Nadeem Raza, Kafil Akhtar, Morten Kam Dahl Dueholm, Asad U. Khan
Summary: The Fap operon of Pseudomonas aeruginosa plays a crucial role in pathoadaptation during pulmonary infection. Its presence increases pathogenesis and biofilm sustainability by modulating bacterial physiology. Targeting amyloids could be a potential clinical application to exploit the pathoadaptive role of Fap in pulmonary infections. The genetic conservation and extracellular exposure of Fap make it a commendable target for interventions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Pharmacology & Pharmacy
Nasser Alhajj, Niall J. O'Reilly, Helen Cathcart
Summary: This review discusses the challenges in developing ciprofloxacin powder for inhalation for CF patients, as well as the rationale behind recent formulations of ciprofloxacin dry powder for inhalation. The challenges include ciprofloxacin lung permeability, overproduction of viscous mucus, and bacterial biofilms. The review also explores current and emerging particle engineering approaches to overcome these challenges.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Microbiology
Xuerui Bao, Ellen Goeteyn, Aurelie Crabbe, Tom Coenye
Summary: D,L-malate enhances ciprofloxacin activity against P. aeruginosa, improving antibiotic therapy in infection environments. The choice of model system influences the outcome of the experiment, emphasizing the importance of using models that closely mimic the in vivo environment. This study confirms the potential of D,L-malate to enhance ciprofloxacin activity against P. aeruginosa-associated infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Review
Nutrition & Dietetics
Bo Wang, Jia Xiang, Binsheng He, Songwen Tan, Wenhu Zhou
Summary: Natural ingredients in modern medicine and pharmaceutical projects often face challenges such as low solubility, poor chemical stability, and low bioavailability. Spray drying technology can improve the properties of natural ingredients and optimize their performance through flexible drug delivery systems. Dry powder inhalation (DPI) has gained significant attention in pharmaceutical research, and this review focuses on the synthesis and application of spray drying for natural ingredients in DPI.
FRONTIERS IN NUTRITION
(2023)
Article
Engineering, Chemical
Dlivan Fattah Aziz, Mostafa Rostamnezhad, Kimia Azad, Yehia Ismail Khalil, Alireza Vatanara
Summary: The aim of this study was to develop a respiratory drug delivery system utilizing montelukast sodium (MTK) microparticles as a target site, using the spray freeze drying (SFD) process. Various sugars and cyclodextrins (CDs) were tested as carriers in order to find compatible excipients for dry powder inhalers (DPIs). The physical characteristics of the collected powders were examined, and the aerodynamic behavior of the particles was assessed. The results showed that CDs were excellent excipients for use with MTK for respiratory drug delivery.
ADVANCED POWDER TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Reham Aljalamdeh, Robert Price, Matthew D. Jones, Albert Bolhuis
Summary: Pseudomonas aeruginosa is a common bacteria causing chronic respiratory infections in cystic fibrosis patients. Biofilms formed by this bacterium are highly resistant to antibiotics. The size of tobramycin particles in dry powder inhalers can influence their efficacy against P. aeruginosa biofilms, with smaller particles showing better results within a 3-hour treatment period.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biology
Fabrice Jean-Pierre, Thomas H. Hampton, Daniel Schultz, Deborah A. Hogan, Marie-Christine Groleau, Eric Deziel, George A. O'Toole
Summary: Interspecies interactions can lead to unexpected microbial phenotypes not observed in monocultures. The lack of in vitro models integrating microbial diversity in the CF airway hinders understanding of why polymicrobial communities are resistant to therapy. We built a mixed community model biofilm, incorporating clinical data, to study the effects of tobramycin treatment on the microorganisms.
Article
Pharmacology & Pharmacy
Gajendra Singh, Albyn Lowe, Athiya Azeem, Shaokoon Cheng, Hak-Kim Chan, Ross Walenga, Agisilaos Kourmatzis
Summary: This study investigated the transport of pharmaceutical dry powder inside an inhaler-like device using high-speed imaging, focusing on the effect of inflow modifications on the dispersion characteristics of agglomerates inside the device. By strategically perturbing airflow conditions, the research observed complete fluidisation of the powder bed and various behaviors such as local turbulence, bimodal fragment size behavior, and flow separation induced by changes in flow behavior. The study's findings provide valuable data for understanding the impact of inflow conditions on a dry powder inhaler-like device and validating computational models.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Hadeel Aburass, Nisreen Dahshan, Hamad Alyami, Affiong Iyire, Eman Zmaily Dahmash
Summary: This study aimed to formulate nanoparticles of date palm extracts as dry powder inhalers (DPIs) for the treatment of cystic fibrosis. Nanoparticles containing 0.05% chitosan showed the highest encapsulation efficiency and demonstrated significant inhibition of Pseudomonas aeruginosa biofilm. This research highlights the potential of date palm nanoparticles as prophylactic adjuvants for cystic fibrosis management using DPI.
JOURNAL OF PHARMACEUTICAL INNOVATION
(2023)
Article
Microbiology
Devin Sindeldecker, Shaurya Prakash, Paul Stoodley
Summary: This study analyzed the zone of killing (ZOK) in Pseudomonas aeruginosa biofilms using both in vitro and in silico methods. The findings revealed that achieving a specific antibiotic concentration versus time constraint (AUC) could completely eradicate bacteria in the biofilms. This suggests that appropriate antibiotic concentrations and treatment duration may be effective against antibiotic-resistant and -tolerant biofilm infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Samuel Nyabam, Zhuo Wang, Thomas Thibault, Ayinde Oluseyi, Rameeza Basar, Lindsay Marshall, Martin Griffin
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2016)
Article
Toxicology
Laura J. Leslie, Pranav Vasanthi Bathrinarayanan, Pamela Jackson, Justin A. Mabiala Ma Muanda, Ross Pallett, Christopher J. P. Stillman, Lindsay J. Marshall
INHALATION TOXICOLOGY
(2017)
Meeting Abstract
Toxicology
Pranav Vasanthi Bathrinarayanan, Lindsay J. Marshall, James E. P. Brown, Laura J. Leslie
TOXICOLOGY LETTERS
(2017)
Review
Pharmacology & Pharmacy
Lindsay J. Marshall, Christopher P. Austin, Warren Casey, Suzanne C. Fitzpatrick, Catherine Willett
DRUG DISCOVERY TODAY
(2018)
Letter
Medicine, General & Internal
Lindsay J. Marshall, Andrew N. Rowan
EUROPEAN JOURNAL OF INTERNAL MEDICINE
(2018)
Article
Toxicology
Pranav Vasanthi Bathrinarayanan, James E. P. Brown, Lindsay J. Marshall, Laura J. Leslie
TOXICOLOGY IN VITRO
(2018)
Review
Pharmacology & Pharmacy
Lindsay J. Marshall, Catherine Willett
DRUG DISCOVERY TODAY
(2018)
Review
Pharmacology & Pharmacy
Lindsay J. Marshall, Wilson Oguejiofor, Rachel S. Willetts, Helen R. Griffiths, Andrew Devitt
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2015)
Article
Multidisciplinary Sciences
Ross Pallett, Laura J. Leslie, Peter. A. Lambert, Ivana Milic, Andrew Devitt, Lindsay J. Marshall
SCIENTIFIC REPORTS
(2019)
Article
Agriculture, Dairy & Animal Science
Lindsay J. Marshall, Marcia Triunfol, Troy Seidle
Article
Agriculture, Dairy & Animal Science
Lindsay J. Marshall, Helder Constantino, Troy Seidle
Summary: Despite adherence to the 3Rs principles, the use of animals in laboratories has not significantly declined. The European Parliament has recently adopted an action plan to phase out the use of animals in research and testing. This article explores the factors needed for the success of this action plan, considering all stakeholders' needs.
Review
Medicine, Research & Experimental
Lindsay J. Marshall, Jarrod Bailey, Manuela Cassotta, Kathrin Herrmann, Francesca Pistollato
Summary: The failure rate for the translation of drugs from animal testing to human treatments remains high at over 92%. Many failures occur due to unexpected safety issues or lack of efficacy. However, the use of innovative tools, like organs-on-chips, in preclinical drug testing has shown promising results in predicting safety events and efficacy. This article reviews disease areas where animal models have failed and suggests the application of more human-relevant approaches to address this issue.
ATLA-ALTERNATIVES TO LABORATORY ANIMALS
(2023)
Review
Pharmacology & Pharmacy
Gillian R. Langley, Ian M. Adcock, Francois Busquet, Kevin M. Crofton, Elena Csernok, Christoph Giese, Tuula Heinonen, Kathrin Herrmann, Martin Hofmann-Apitius, Brigitte Landesmann, Lindsay J. Marshall, Emily McIvor, Alysson R. Muotri, Fozia Noor, Katrin Schutte, Troy Seidle, Anja van de Stolpe, Hilde Van Esch, Catherine Willett, Grzegorz Woszczek
DRUG DISCOVERY TODAY
(2017)
Meeting Abstract
Respiratory System
Ross Pallett, Lindsay J. Marshall
EUROPEAN RESPIRATORY JOURNAL
(2016)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)