期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 504, 期 1-2, 页码 1-10出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2016.03.021
关键词
Lipid; Dendrimer; Hybrid nanoparticles; Antibiotic; Sustained release; Bacterial resistance; MRSA
资金
- University of KwaZulu-Natal (UKZN)
- National Research Foundation (NRF) of South Africa
The purpose of this study was to explore the preparation of a new lipid-dendrimer hybrid nanoparticle (LDHN) system to effectively deliver vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) infections. Spherical LDHNs with particle size, polydispersity index and zeta potential of 52.21 +/- 0.22 nm, 0.105 +/- 0.01, and -14.2 +/- 1.49 mV respectively were prepared by hot stirring and ultrasonication using Compritol 888 ATO, G4 PAMAM- succinamic acid dendrimer, and Kolliphor RH-40. Vancomycin encapsulation efficiency (%) in LDHNs was almost 4.5-fold greater than in lipid-polymer hybrid nanoparticles formulated using Eudragit RS 100. Differential scanning calorimetry and Fourier transform-infrared studies confirmed the formation of LDHNs. The interactions between the drug-dendrimer complex and lipid molecules using in silico modeling revealed the molecular mechanism behind the enhanced encapsulation and stability. Vancomycin was released from LDHNs over the period of 72 h with zero order kinetics and super case II transport mechanism. The minimum inhibitory concentration (MIC) against S. aureus and MRSA were 15.62 mu g/ml and 7.81 mu g/ml respectively. Formulation showed sustained activity with MIC of 62.5 mu g/ml against S. aureus and 500 mu g/ml against MRSA at the end of 72 and 54 h period respectively. The results suggest that the LDHN system can be an effective strategy to combat resistant infections. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据