Review
Biochemistry & Molecular Biology
Agnieszka Markowska, Adam Roman Markowski, Iwona Jarocka-Karpowicz
Summary: 6-aminohexanoic acid is not only a omega-amino acid used clinically as an antifibrinolytic drug, but also plays a significant role in chemical synthesis and the synthetic fiber industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Angel Garcia-Raso, Angel Terron, Juan J. Fiol, Adela Lopez-Zafra, Gemma Pico, Ezequiel M. Vazquez-Lopez, Miquel Barcelo-Oliver, Antonio Frontera
Summary: In this manuscript, we report the synthesis and structural characterization of five pyrimidine amino acid/peptide modified derivatives. DFT calculations were used to study the formation of head-to-tail or head-to-head 1D supramolecular polymers in the solid state. Other assemblies like antiparallel pi-stacking and anion center dot center dot center dot pi-hole dimers have also been studied and compared.
Review
Engineering, Chemical
Yuko Tsuda, Koushi Hidaka, Keiko Hojo, Yoshio Okada
Summary: Plasmin, a serine protease responsible for fibrinolysis pathway and pathology events, has inhibitors designed to bind to its active site, preventing it from digesting substrates. These inhibitors have potential as anti-inflammatory and anti-cancer agents, with second-generation inhibitors optimized for both activity and selectivity. This review focuses on the relationship between Plasmin inhibitors' inhibitory activity and structure for design and clinical application purposes.
Article
Biochemistry & Molecular Biology
Ekaterina Blinova, Dmitry Pakhomov, Denis Shimanovsky, Marina Kilmyashkina, Yan Mazov, Tatiana Demura, Vladimir Drozdov, Dmitry Blinov, Olga Deryabina, Elena Samishina, Aleksandra Butenko, Sofia Skachilova, Alexey Sokolov, Olga Vasilkina, Bashar A. Alkhatatneh, Olga Vavilova, Andrey Sukhov, Daniil Shmatok, Ilya Sorokvasha, Oxana Tumutolova, Elena Lobanova
Summary: The study demonstrated that LHT-8-17 accelerated wound healing in diabetic animals, reduced inflammatory cytokine levels and oxidative stress activity, enhanced antioxidant capacity, promoted cell proliferation, increased tissue vascularization, and exhibited antimicrobial properties, suggesting its potential as a promising pharmacological agent for diabetic wound topical treatment.
Review
Medicine, General & Internal
Stuart Hastings, Paul S. Myles, Robert L. Medcalf
Summary: Surgical site infections (SSIs) are a common economic burden that increase patient morbidity and mortality. Although antibiotic prophylaxis is the primary prevention intervention, it may be less effective due to emerging antibiotic resistant organisms. Exploration of novel therapies, such as tranexamic acid, as adjunct to antibiotics is warranted to reduce SSIs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Hematology
Soren Hauge Okholm, Jan Krog, Anne-Mette Hvas
Summary: This systematic review suggests that tranexamic acid (TXA) has an anti-inflammatory effect in orthopaedic patients, as evidenced by decreased levels of C-reactive protein and interleukin-6. However, this effect was not observed in patients undergoing cardiac surgery, pediatric craniosynostosis patients, or rheumatoid arthritis patients. The form of TXA administration did not impact the inflammatory response.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2022)
Article
Chemistry, Medicinal
Tanmaye Nallan Chakravarthula, Ziqian Zeng, Nathan J. Alves
Summary: This study explores the effect of multivalent benzamidine inhibitors with varying valency and linker length on plasmin inhibition. The findings suggest that increasing valency and decreasing linker length can enhance the inhibition of plasmin, providing guidance for designing multivalent inhibitors.
Article
Biotechnology & Applied Microbiology
Lisa Bretschneider, Martin Wegner, Katja Buehler, Bruno Buehler, Rohan Karande
Summary: A bioprocess for synthesizing 6-aminohexanoic acid (6AHA) was developed using a mixed-species approach with promising results. The combination of Pseudomonas taiwanensis and Escherichia coli strains allowed for high conversion and yield of 6AHA under environmentally benign conditions.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Veterinary Sciences
Gerard Olivares, Mellora Sharman, Rachel Miller, Caroline Kisielewicz, Mayank Seth
Summary: The aim of this feasibility study was to evaluate the use of tranexamic acid alongside standard therapy in dogs with primary immune thrombocytopenia (ITP). The results showed that tranexamic acid did not provide a clinical benefit in treating ITP and was associated with a high incidence of vomiting.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Adam Miszta, Dana Huskens, Demy Donkervoort, Molly J. M. Roberts, Alisa S. Wolberg, Bas de Laat
Summary: Fibrinolysis is a crucial process in wound healing, with plasmin playing a central role in dissolving clots. Recent advancements have enabled the quantification of plasmin generation (PG) and inhibition kinetics during fibrinolysis. Understanding plasmin kinetics aids in identifying fibrinolytic dysfunction and investigating the link between abnormal fibrin dissolution and disease pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Lavleen Mader, John J. Hayward, Lisa A. Porter, John F. Trant
Summary: This study reports an improved and refocused synthesis method for CDK2 inhibitors, allowing for effective exploration of substituents at the O-6 position.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Hematology
Adam Miszta, Homa K. Ahmadzia, Naomi L. C. Luban, Shuhui Li, Dong Guo, Lori A. Holle, Jeffrey S. Berger, Andra H. James, Jogarao V. S. Gobburu, John van den Anker, Bas de Laat, Alisa S. Wolberg
Summary: The study developed an assay to measure plasmin generation (PG) kinetics in human plasma, and determined the effects of TXA on PG. The results showed that TXA dose-dependently prolonged clot lysis time and delayed and reduced PG, demonstrating its inhibition of PG in women undergoing cesarean delivery.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Pharmacology & Pharmacy
Ting Zhang, Hua Feng, Wei Xiao, Jingsheng Li, Qinghai Liu, Xuexin Feng, Dezhou Qi, Xiaotong Fan, Yongzhi Shan, Tao Yu, Guoguang Zhao, Tianlong Wang
Summary: Prophylactic administration of TXA combined with TEG-guided hemostatic algorithm can be an effective multimodal coagulation strategy for reducing allogeneic transfusion requirements during pediatric resective epilepsy surgery.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Jue T. Wang, Samir C. Seshadri, Carolyn G. Butler, Steven J. Staffa, Anna S. Kordun, Karina E. Lukovits, Susan M. Goobie
Summary: This study evaluates the current practice of tranexamic acid administration for pediatric neurosurgery at a U.S. tertiary care pediatric hospital over a five-year period. The findings suggest that TXA administration is feasible and should be considered for pediatric neurosurgical cases where potential blood loss is a concern.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Critical Care Medicine
Carla B. Rynkowski, Vanessa Hegele, Pedro Henrique Rigotti Soares, Monica Lopes Tonello, Leticia Peterson, Frederico Klein Gomes, Alejandro A. Rabinstein, Leonardo S. L. Bastos, Ricardo Turon, Bruno Goncalves, Cassia Righy, Fernando A. Bozza, Pedro Kurtz
Summary: This study investigated the effects of tranexamic acid on long-term functional outcomes of patients with aneurysmal subarachnoid hemorrhage (aSAH). The results showed that the use of tranexamic acid before aneurysm occlusion did not improve the functional outcomes of aSAH patients.
NEUROCRITICAL CARE
(2023)
Article
Biochemistry & Molecular Biology
Anna Sadowska, Ewa Zapora, Diana Sawicka, Katarzyna Niemirowicz-Laskowska, Arkadiusz Surazynski, Katarzyna Sulkowska-Ziaja, Katarzyna Kala, Marcin Stocki, Marek Wolkowycki, Slawomir Bakier, Anna Pawlik, Magdalena Jaszek, Bozena Muszynska, Halina Car
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Iwona Jarocka-Karpowicz, Agnieszka Markowska
Summary: Cancer is a major concern in modern medicine due to the limited efficacy of current therapies. Natural compounds, such as jasmonic acid and its derivatives, are being explored for their potential in cancer prevention and treatment. The anti-tumor activity of jasmonates is attributed to mechanisms such as altering cellular ATP levels, inducing re-differentiation through MAPKs, and promoting apoptosis through reactive oxygen species. Jasmonates can also show synergistic effects when used in combination with other cancer drugs like cisplatin and paclitaxel.
Article
Chemistry, Physical
Wojciech Zieba, Joanna Czarnecka, Tomasz Rusak, Monika Zieba, Artur P. Terzyk
Summary: The study presents the cytotoxicity of single-walled carbon nanohorn (SWCNH) oxidized materials on A549 and HDF cell lines, as well as the hemocompatibility on human and porcine blood samples. Results show correlation between specific surface area of materials and platelet aggregation, and the I-D/I-G ratio from Raman spectra with hemoglobin release from erythrocytes.
Article
Physiology
Agata Golaszewska, Tomasz Misztal, Natalia Marcinczyk, Ewa Chabielska, Tomasz Rusak
Summary: The study shows that adrenaline can enhance platelet activation and thrombus formation in the presence of subthreshold collagen, leading to increased platelet aggregation and formation of denser platelet-fibrin clots. These effects are associated with a decrease in fibrinolysis rate, which can be reduced by commonly used antiplatelet drugs.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Iwona Jarocka-Karpowicz, Agnieszka Markowska
Summary: Jasmonates, such as jasmonic acid and methyl jasmonate, are natural compounds with broad spectrum of activities, including anti-cancer, anti-inflammatory, and cosmetic effects. Studies on their biological activity in mammals have shown potential for inflammation treatment due to their structural similarities to prostaglandins. Furthermore, jasmonates are being considered as potential anti-cancer drugs when used alone or in combination with other chemotherapy agents, and may also have applications in cosmetics and dermatology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Natalia Marcinczyk, Tomasz Misztal, Anna Gromotowicz-Poplawska, Agnieszka Zebrowska, Tomasz Rusak, Piotr Radziwon, Ewa Chabielska
Summary: The study introduced the PECAM-1/thrombus ratio as a parameter indicating platelet activation status, verified its usefulness in animal and human studies using a flow chamber model, and found that acetylsalicylic acid (ASA) could decrease thrombus area while increasing PECAM-1/thrombus ratio in a dose-dependent manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Michal Biernacki, Malgorzata Michalina Brzoska, Agnieszka Markowska, Malgorzata Galazyn-Sidorczuk, Bogdan Cylwik, Agnieszka Gegotek, Elzbieta Skrzydlewska
Summary: The study concludes that CBD has a protective effect against oxidative stress caused by UVB radiation, enhancing antioxidant capacity and reducing lipid peroxidation and protein modifications. CBD showed to counteract the down-regulation of 2-arachidonylglycerol induced by UV, promoting antioxidant and anti-inflammatory effects.
Article
Biochemistry & Molecular Biology
Adam Kazberuk, Magda Chalecka, Jerzy Palka, Arkadiusz Surazynski
Summary: Nonsteroidal anti-inflammatory drugs (NSAIDs) may induce apoptosis through the activation of PPAR gamma and the proline metabolism enzyme PRODH/POX in breast cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Agnieszka Markowska, Adam Roman Markowski, Iwona Jarocka-Karpowicz
Summary: 6-aminohexanoic acid is not only a omega-amino acid used clinically as an antifibrinolytic drug, but also plays a significant role in chemical synthesis and the synthetic fiber industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Magda Chalecka, Adam Kazberuk, Jerzy Palka, Arkadiusz Surazynski
Summary: The study focuses on the role of amino acid metabolism, particularly nonessential amino acids, in the regulation of various cellular processes. P5C serves as a central component in energy production and interconversion of carbons within the cell, linking multiple metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Agnieszka Gegotek, Sinemyiz Atalay, Adam Wronski, Agnieszka Markowska, Elzbieta Skrzydlewska
Summary: The development of psoriasis is influenced by oxidative stress and inflammation. This study analyzed the effects of PsV and PsA on MMPs, TIMPs, and angiogenesis factors, as well as the impact of CBD on these parameters. Results suggest that CBD may serve as an antiangiogenic factor in psoriatic keratinocytes, reducing the proinflammatory effects of UVB and providing partial protection for healthy keratinocytes.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Biochemistry & Molecular Biology
Adam Kazberuk, Magda Chalecka, Jerzy Palka, Katarzyna Bielawska, Arkadiusz Surazynski
Summary: This study found that non-steroidal anti-inflammatory drugs (NSAIDs) induce apoptosis in breast cancer cells through the activation of peroxisome proliferator-activated receptor-gamma (PPAR gamma) and the generation of reactive oxygen species (ROS) via proline dehydrogenase/proline oxidase (PRODH/POX). The effect of NSAIDs on cell apoptosis is independent of cyclooxygenase-2 (COX-2) inhibition. Additionally, the study showed that the role of PRODH/POX in NSAID-induced apoptosis is more pronounced in breast cancer cells with PRODH/POX knockout. The findings suggest a potential therapeutic strategy for cancer treatment using NSAIDs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Misztal, Agata Golaszewska, Natalia Marcinczyk, Maria Tomasiak-Lozowska, Malgorzata Szymanowska, Ewa Chabielska, Tomasz Rusak
Summary: This study investigated the effects of three plant-derived polyphenols (Que, EGCG, and Resv) on hemostatic abnormalities caused by HOCl. The results showed that Que and EGCG could eliminate HOCl-induced inhibition of platelet aggregation, while Resv enhanced the inhibitory effect of HOCl on platelets.
Article
Engineering, Biomedical
Hetmann Anna, Szymczak Bartosz, Czarnecka Joanna, Rusak Tomasz, Wisniewski Marek, Wujak Magdalena, Roszek Katarzyna
Summary: Purinergic signaling has emerged as a crucial target for anti-cancer therapies, and the nanobiocatalytic system of AK-GO has been developed to modify the purinergic signaling components in lung carcinoma cells. The immobilized adenylate kinase effectively controls nucleotide balance and decreases adenosine concentration, leading to reduced proliferation and migration ability of cancer cells. The developed AK-GO nanobiocatalytic system shows good compatibility for biomedical applications.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Materials Science, Biomaterials
Monika Zieba, Tomasz Rusak, Tomasz Misztal, Wojciech Zieba, Natalia Marcinczyk, Joanna Czarnecka, Samer Al-Gharabli, Joanna Kujawa, Artur P. Terzyk
Summary: This study aims to design new materials with high blood compatibility and conducts hemocompatibility studies using poly(vinylidene fluoride) (PVDF) composites. Plasma-surface modifications are used to enhance hemocompatibility, and the optimal samples are selected through an optimization procedure. The results show that nitrogen plasma treatment is the best method to improve blood compatibility, with low platelet adhesion, no cytotoxicity, and excellent performance in thromboelastometry and hemolysis tests. The new surfaces have potential applications in cardiological and circulatory system implants and other blood-related biomaterials.
BIOMATERIALS ADVANCES
(2022)
