Article
Biochemistry & Molecular Biology
Ines Silva, Joao Solas, Rui Pinto, Vanessa Mateus
Summary: This study successfully developed and validated a chronic TNBS-induced colitis mouse model. After week 4, clinical and inflammation signs remained stable, suggesting the model develops within 4 weeks.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dengchao Xie, Xin Zhou, Bo Xiao, Lian Duan, Zhenhua Zhu
Summary: This study fabricated silk fibroin-based nanoparticles loaded with resveratrol (RSV) and functionalized with Pluronic F127 (PF-127) to enhance mucus-penetrating capacity and anti-inflammatory effects. The functionalized nanoparticles showed improved mucus-penetrating property and enhanced suppression of proinflammatory cytokine secretion. In a colitis model, the PF-127 functionalized nanoparticles demonstrated better therapeutic outcomes compared to other counterparts.
Article
Pharmacology & Pharmacy
Patricia Diez-Echave, Antonio Jesus Ruiz-Malagon, Jose Alberto Molina-Tijeras, Laura Hidalgo-Garcia, Teresa Vezza, Laura Cenis-Cifuentes, Maria Jesus Rodriguez-Sojo, Jose Luis Cenis, Maria Elena Rodriguez-Cabezas, Alba Rodriguez-Nogales, Julio Galvez, Antonio Abel Lozano-Perez
Summary: Inflammatory bowel disease (IBD) is a chronic inflammatory disorder with limited effectiveness in current pharmacological treatments. Research suggests that loading quercetin in silk fibroin nanoparticles may have anti-inflammatory effects and serve as a promising treatment for IBD.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Oncology
Elia Bari, Massimo Serra, Mayra Paolillo, Eric Bernardi, Sara Tengattini, Filippo Piccinini, Cristina Lanni, Marzio Sorlini, Giovanni Bisbano, Enrica Calleri, Maria Luisa Torre, Sara Perteghella
Summary: The paper discusses the active targeting of tumor cells using integrin-targeted nanoparticles, specifically using silk fibroin nanoparticles (SFNs) functionalized with RGD. This strategy effectively boosts antitumor activity and reduces damage to healthy organs by actively internalizing into tumor cells overexpressing integrin receptors. The functionalization of SFNs with cRGD provides in vitro active targeting for site-specific delivery of anticancer drugs, showing increased uptake by tumor cells and potential for improving anticancer therapies.
Article
Biochemistry & Molecular Biology
Ying Shen, Xinyu Wang, Binbin Li, Yajin Guo, Kuo Dong
Summary: This study incorporated nanoparticles into a scaffold to improve hemostatic properties and stimulate cell adhesion. The scaffold loaded with nanoparticles exhibited higher blood clotting index and promoted platelets adhesion. In addition, the scaffold loaded with nanoparticles showed stimulation effects on cell proliferation, migration, and adhesion. Therefore, the scaffold loaded with nanoparticles has great potential as a wound dressing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Ines Silva, Priscila Mendes, Sofia Guerra, Rui Pinto, Vanessa Mateus
Summary: Inflammatory bowel disease (IBD) is a chronic and relapsing inflammatory response in the gastrointestinal tract. Current treatments do not cure the underlying pathogenic mechanism, leading to a need for new and effective pharmacological approaches. Preclinical studies show that topiramate has potential anti-inflammatory effects. This study investigates the effect of topiramate in a chronic TNBS-induced colitis model in rodents, demonstrating its ability to reduce colonic damage and normalize stool consistency and anus appearance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Vishal Khatri, Ramaswamy Kalyanasundaram
Summary: This review focuses on the association between IBD and gut inflammasome, as well as recent advances in research and therapeutic strategies for IBD, discussing inflammasomes and their components, outcomes from experimental animals and human studies, inflammasome inhibitors, and developments in inflammasome-targeted therapies for IBD.
Review
Biochemistry & Molecular Biology
HyunTaek Jung, Jae Seok Kim, Keum Hwa Lee, Kalthoum Tizaoui, Salvatore Terrazzino, Sarah Cargnin, Lee Smith, Ai Koyanagi, Louis Jacob, Han Li, Sung Hwi Hong, Dong Keon Yon, Seung Won Lee, Min Seo Kim, Paul Wasuwanich, Wikrom Karnsakul, Jae Il Shin, Andreas Kronbichler
Summary: Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract that mainly affects young people. Recent studies have shown that microRNAs (miRNAs) play an important role in the pathogenesis, diagnosis, and treatment of IBD.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Guihua Chen, Jun Shen
Summary: Inflammatory bowel disease (IBD) is a global disease with high morbidity and medical costs. Early diagnosis and intervention are crucial for controlling its progression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Medicine, General & Internal
Elisabetta Antonelli, Gabrio Bassotti, Marta Tramontana, Katharina Hansel, Luca Stingeni, Sandro Ardizzone, Giovanni Genovese, Angelo Valerio Marzano, Giovanni Maconi
Summary: This review summarizes the dermatologic manifestations occurring in patients with inflammatory bowel diseases, including specific manifestations, cutaneous disorders associated with IBDs, reactive mucocutaneous manifestations of IBDs, mucocutaneous conditions secondary to treatment, and manifestations due to nutritional malabsorption. An accurate dermatological examination is crucial in all IBD patients, especially in candidates to biologic therapies where drug-induced cutaneous reactions may be clinically relevant.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, General & Internal
Silvia Salvatori, Francesco Baldassarre, Michelangela Mossa, Giovanni Monteleone
Summary: Long COVID is common in patients with inflammatory bowel diseases (IBD), with fatigue being the most frequent symptom. It does not influence the frequency of IBD relapses, but is more prevalent in female patients.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Engineering, Biomedical
Shengzhi Zou, Xiang Yao, Huili Shao, Rui L. Reis, Subhas C. Kundu, Yaopeng Zhang
Summary: Silk fibroin is a promising biomaterial due to its good biocompatibility, availability, and mechanical properties. Nonmulberry silk fibroin, extracted from nonmulberry silkworm silk, has unique arginine-glycine-aspartic acid sequences that enhance cell adhesion. This makes it more suitable for cell culture and tissue regeneration applications.
ACTA BIOMATERIALIA
(2022)
Review
Pharmacology & Pharmacy
Dulce C. Macias-Ceja, Maria D. Barrachina, Dolores Ortiz-Masia
Summary: Chronic inflammation is associated with excessive deposition of extracellular matrix and leads to fibrosis. Intestinal fibrosis is a common complication of inflammatory bowel disease (IBD), and deregulated autophagy is linked to fibrosis progression. Understanding the role of autophagy in fibrosis may provide new targets for antifibrotic therapy. This review focuses on the relevance of autophagy in fibrosis, with a special emphasis on IBD-related fibrosis.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Nutrition & Dietetics
Srishti Saha, Neha Patel
Summary: Inflammatory bowel disease (IBD) is a chronic disorder caused by enteric inflammation in a genetically susceptible host. Diet is widely accepted as an important factor in the pathogenesis of IBD, and various dietary components have been studied for their roles in causing enteric inflammation. Different diets, such as exclusive enteral nutrition, specific carbohydrate diet, and low FODMAPs diet, have also been explored for their effects on IBD patients. This review summarizes the current evidence on the relationship between dietary components and IBD, and provides guidance for dietary modifications in managing IBD.
Article
Nanoscience & Nanotechnology
Alessandra Maria Bossi, Alessio Bucciarelli, Devid Maniglio
Summary: Nanosized biomimetics prepared by molecular imprinting are showing potential as plastic antibodies in medicine for cell imaging and targeted therapies. Utilizing natural polymer silk fibroin to synthesize biocompatible MIP SF-NPs demonstrates high affinity recognition sites and biocompatibility.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Clinical Neurology
Teresa Vezza, Jose Alberto Molina-Tijeras, Rafael Gonzalez-Cano, Alba Rodriguez-Nogales, Federico Garcia, Julio Galvez, Enrique J. Cobos
Summary: Abdominal pain is a common symptom in patients with inflammatory bowel disease (IBD) and has a significant impact on their quality of life. Minocycline, a broad-spectrum tetracycline antibiotic, has been identified as a potential therapy for managing visceral pain in IBD-induced colitis in mice.
Article
Immunology
Zaied Ahmed Bhuyan, M. Arifur Rahman, Muralidhara Rao Maradana, Ahmed M. Mehdi, Anne-Sophie Bergot, Davide Simone, Marya El-Kurdi, Jose Garrido-Mesa, Cheng Bang Benjamin Cai, Amy J. Cameron, Aimee L. Hanson, Hendrik J. Nel, Tony Kenna, Paul Leo, Linda Rehaume, Matthew A. Brown, Francesco Ciccia, Ranjeny Thomas
Summary: Loss-of-function variants in RUNX3 are associated with ankylosing spondylitis (AS) and lead to immune dysregulation, intestinal dysbiosis, and inflammation. In AS, CD4-IEL cells and CD4+CD8+ T cells are decreased, while Treg cells are increased. TCR signaling dysfunction plays a role in the intestinal T-cell immunodeficiency observed in AS.
CLINICAL IMMUNOLOGY
(2023)
Article
Nutrition & Dietetics
Maria Jesus Rodriguez-Sojo, Jorge Garcia-Garcia, Antonio Jesus Ruiz-Malagon, Patricia Diez-Echave, Laura Hidalgo-Garcia, Jose Alberto Molina-Tijeras, Elena Gonzalez-Lozano, Laura Lopez-Escanez, Maria Elena Rodriguez-Cabezas, Maria Jose Rodriguez-Sanchez, Alba Rodriguez-Nogales, Cristina Mediavilla, Julio Galvez
Summary: Limosilactobacillus fermentum CECT5716, a probiotic strain isolated from human milk, has shown beneficial effects on different gastrointestinal disorders and immune responses. In this study, its effects on an irritable bowel syndrome (IBS) rat model were assessed, showing improvements in behavior, hypersensitivity, inflammation, intestinal barrier integrity, and gut microbiota composition. These findings suggest the potential clinical use of Limosilactobacillus fermentum CECT5716 for IBS management.
Article
Nutrition & Dietetics
Enrique Guillamon, Nuria Mut-Salud, Maria Jesus Rodriguez-Sojo, Antonio Jesus Ruiz-Malagon, Antonio Cuberos-Escobar, Antonio Martinez-Ferez, Alba Rodriguez-Nogales, Julio Galvez, Alberto Banos
Summary: This study demonstrates the potential anti-proliferative and anti-inflammatory effects of onion-derived metabolites PTS and PTSO on various human tumor lines. These compounds have the ability to induce apoptosis and reduce the levels of pro-inflammatory cytokines, suggesting their promising role in cancer prevention and/or treatment.
Article
Nutrition & Dietetics
Jorge Garcia-Garcia, Carlos Gracian, Alberto Banos, Enrique Guillamon, Julio Galvez, Alba Rodriguez-Nogales, Juristo Fonolla
Summary: This study aimed to evaluate the impact of a garlic and onion extract concentrate on the incidence of respiratory tract infections in elderly patients. The results showed that the extract is safe to use and significantly reduces the occurrence of respiratory infections, as well as the duration and number of associated symptoms.
Article
Biochemistry & Molecular Biology
Jose Alberto Molina-Tijeras, Antonio Jesus Ruiz-Malagon, Laura Hidalgo-Garcia, Patricia Diez-Echave, Maria Jesus Rodriguez-Sojo, Maria de la Luz Cadiz-Gurrea, Antonio Segura-Carretero, Jose Perez del Palacio, Maria Reyes Gonzalez-Tejero, Maria Elena Rodriguez-Cabezas, Julio Galvez, Alba Rodriguez-Nogales, Teresa Vezza, Francesca Algieri
Summary: Obesity, a global public health problem, is increasing in prevalence rate. Plant-based treatments, such as Lavandula multifida extract (LME), have been gaining attention as a means to manage obesity and its comorbidities. In this study, LME was found to reduce weight gain, improve insulin sensitivity and glucose tolerance, decrease inflammation in the liver and adipose tissue, regulate gut permeability, and reduce oxidative stress. These findings suggest that LME may be a promising supplementary approach for managing obesity and its comorbidities.
Article
Biochemistry & Molecular Biology
Maria Jesus Rodriguez-Sojo, Antonio Jesus Ruiz-Malagon, Laura Hidalgo-Garcia, Jose Alberto Molina-Tijeras, Patricia Diez-Echave, Laura Lopez-Escanez, Lucrezia Rosati, Elena Gonzalez-Lozano, Laura Cenis-Cifuentes, Jorge Garcia-Garcia, Federico Garcia, Inaki Robles-Vera, Miguel Romero, Juan Duarte, Jose Luis Cenis, Antonio Abel Lozano-Perez, Julio Galvez, Maria Elena Rodriguez-Cabezas, Alba Rodriguez-Nogales
Summary: Morus alba L. leaf extract has beneficial effects on high-fat diet-induced obesity in mice by reducing weight gain, attenuating lipid accumulation, increasing glucose sensitivity, and improving obesity-associated inflammation. These effects are possibly due to the extract's antioxidant properties. Moreover, the extract also mitigates gut dysbiosis, as evidenced by the restoration of the Firmicutes/Bacteroidota ratio and the decrease in plasma lipopolysaccharide levels.
Article
Infectious Diseases
Jorge Garcia-Garcia, Patricia Diez-Echave, Maria Eugenia Yuste, Natalia Chueca, Federico Garcia, Jose Cabeza-Barrera, Emilio Fernandez-Varon, Julio Galvez, Manuel Colmenero, Maria Elena Rodriguez-Cabezas, Alba Rodriguez-Nogales, Rocio Moron
Summary: The number of ICU patients and antibiotic treatments has increased due to SARS-CoV-2 infection, leading to a rise in multi-drug resistant bacteria (MDRB) colonization. As these bacteria mainly come from gut microbiota, studying its composition is crucial. This pilot study aims to identify bacteria biomarkers for predicting MDRB colonization risk in SARS-CoV-2 patients in ICUs.
Article
Chemistry, Medicinal
Maria Alejandra Asensio Ruiz, Angela Alonso Garcia, Maria de la Luz Bravo-Ferrer Moreno, Iria Cebreiros-Lopez, Jose Antonio Noguera-Velasco, Antonio Abel Lozano-Perez, Teresa Martinez Martinez
Summary: Nuclear imaging, using radiolabeled nanomaterials as imaging probes, is a valuable tool for medical diagnosis. This study developed a direct method for radiolabeling FITC-tagged silk fibroin nanoparticles (SFN) and demonstrated its stability and efficiency in biological media. The dual-labeling method showed potential for further investigations of SFN biodistribution in vivo.
Article
Chemistry, Multidisciplinary
Beatriz Torres-Herrero, Ilaria Armenia, Maria Alleva, Laura Asin, Sonali Correa, Cecilia Ortiz, Yilian Fernandez-Afonso, Lucia Gutierrez, Jesus M. de la Fuente, Lorena Betancor, Valeria Grazu
Summary: Here, researchers have developed nanohybrids (nHs) for remote activation of a therapeutic enzyme in Directed Enzyme Prodrug Therapy (DEPT). The nanosized hybrids, composed of magnetic nanoparticles (MNPs) and horseradish peroxidase (HRP) encapsulated in biomimetic silica, were optimized for efficient remote activation of the therapeutic enzyme. The application of alternating magnetic fields (AMFs) to MNPs increased the bioconversion rate of HRP, demonstrating the possibility of enzyme nanoactuation with co-encapsulated MNPs. In vitro and in vivo experiments showed that the enzyme-loaded nHs triggered cell death and significantly reduced tumor volume growth when exposed to AMFs, highlighting the potential of this spatiotemporally controlled DEPT strategy to overcome off-target effects.
Article
Biochemical Research Methods
Christian Castro-Hinojosa, Susel Del Sol-Fernandez, Eduardo Moreno-Antolin, Beatriz Martin-Gracia, Jesus G. Ovejero, Jesus Martinez de la Fuente, Valeria Grazu, Raluca M. Fratila, Maria Moros
Summary: In this study, we describe a simple and efficient method for the functionalization of proteins with magnetic nanoparticles (MNPs) using metal affinity binding. Our method preserves the functionality and colloidal stability of the MNPs while allowing for the precise control of protein orientation and density. This approach has potential applications in various fields.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Xando Diaz-Villamarin, Emilio Fernandez-Varon, Michelle Carolina Rojas Romero, Jose Luis Callejas-Rubio, Jose Cabeza-Barrera, Alba Rodriguez-Nogales, Julio Galvez, Rocio Moron
Summary: This study assesses the clinical impact of azathioprine dose tailoring based on TPMT genotyping and finds that dose reduction in TPMT intermediate metabolizer patients is associated with lower toxicity events and fewer dose adjustments.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
