Review
Medicine, General & Internal
Herve Boutal, Christian Moguet, Lilas Pommies, Stephanie Simon, Thierry Naas, Herve Volland
Summary: The global spread of antimicrobial resistant bacteria poses a significant public health concern. Detecting and identifying resistance mechanisms is challenging, but lateral flow assays (LFA) have emerged as cost-effective and rapid tools to combat antimicrobial resistance. This review examines the versatility and performance of LFA for detecting antimicrobial resistance, focusing on beta-lactamase triggers. It also discusses potential modifications to broaden clinical sensitivity and improve sample handling processes. Additionally, the use of LFA in detecting amplified genetic determinants of antimicrobial resistance is explored.
Article
Infectious Diseases
Noah Obeng-Nkrumah, Dennis Schroder Hansen, Georgina Awuah-Mensah, Nana Kweiba Blankson, Niels Frimodt-Moller, Mercy Jemima Newman, Japheth Awuletey Opintan, Karen Angeliki Krogfelt
Summary: A cross-sectional survey was conducted in eight Ghanaian communities to investigate the extent of intestinal colonization with 3rd-generation cephalosporin-resistant Enterobacterales. The study found that 50.4% of the participants carried 3rd-generation cepha-losporin-resistant E. coli and K. pneumoniae. Most of these were ESBL-producing E. coli, carrying CTX-M genes, mainly CTX-M-15. The presence of a household toilet facility was significantly associated with a reduced risk of intestinal colonization.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Rumyana Markovska, Petya Stankova, Temenuga Stoeva, Dobrinka Ivanova, Daniela Pencheva, Radka Kaneva, Lyudmila Boyanova
Summary: This study investigated ESBL-producing Enterobacterales isolates in patients and hospital staff in two Bulgarian hospitals, finding a high prevalence of these bacteria with various resistance genes and plasmid types, indicating a significant presence of multidrug-resistant bacteria in the environment.
Article
Medicine, General & Internal
Camille Gonzalez, Saoussen Oueslati, Laura Biez, Laurent Dortet, Thierry Naas
Summary: Early detection of multidrug resistant bacteria is crucial for infection control and proper treatment. The EasyScreen(TM) ESBL/CPO Detection Kit is a highly efficient real-time PCR assay for detecting colistin resistance and beta-lactamase genes.
Article
Microbiology
Elzbieta M. Stefaniuk, Aleksandra Kozinska, Izabela Wasko, Anna Baraniak, Stefan Tyski
Summary: A total of 65 colistin-resistant Enterobacterales isolates were analyzed in this study, with 45 identified as ESBL producers. CTX-M-1-like enzymes were the most common ESBLs. Additionally, one E. coli and seven Klebsiella pneumoniae strains produced carbapenemases.
POLISH JOURNAL OF MICROBIOLOGY
(2021)
Article
Microbiology
Rumyana Markovska, Petya Stankova, Temenuga Stoeva, Marianna Murdjeva, Yulia Marteva-Proevska, Dobrinka Ivanova, Maryia Sredkova, Atanaska Petrova, Kalina Mihova, Lyudmila Boyanova
Summary: The gastrointestinal tract serves as a significant reservoir for high-risk Enterobacteria clones, which contribute to antimicrobial resistance in hospitals. This study conducted in Bulgaria identified various cefotaxime-resistant Enterobacterales isolates in fecal samples of patients, including ESBL, carbapenemase, and plasmidic AmpC producers. High-risk clones of Klebsiella pneumoniae and Escherichia coli were detected, indicating the importance of routine screenings and infection control measures, particularly amid the COVID-19 pandemic.
Article
Engineering, Environmental
Zhiguo Zhang, Guoqing Zhang, Feng Ju
Summary: Monitoring clinically prevalent antibiotic-resistant bacteria in high bacterial community diversity is challenging. This study introduces a new method called culture enriched phenotypic metagenomics, which effectively monitors antibiotic-resistant bacteria in wastewater treatment plants. The results show the widespread presence and potential dissemination of antibiotic resistance genes in this environment.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Liqiang Hu, Runqiu Liu, Zheng Ma, Tao Yu, Ziyao Li, Yingqiu Zou, Chang Yuan, Fangfang Chen, Hexin Xie
Summary: A fluorogenic probe has been developed for the specific detection of IMP-1 beta-lactamase activity, featuring a unique trans-acetylamino cephalosporin as an enzymatic recognition moiety. This probe exhibits excellent selectivity to IMP-1 beta-lactamase over other beta-lactamases, with over 30-fold enhancement in fluorescence intensity upon activation by IMP-1 beta-lactamase, allowing for high sensitivity reporting of enzyme activity.
CHEMICAL COMMUNICATIONS
(2021)
Article
Microbiology
Pranita D. Tamma, Tiffeny T. Smith, Ayomikun Adebayo, Sara M. Karaba, Emily Jacobs, Teresa Wakefield, Kelly Nguyen, Natalie N. Whitfield, Patricia J. Simner
Summary: Understanding the prevalence of bla(CTX-M) genes in specific Gram-negative bacterial species is crucial for guiding antibiotic treatment. Carbapenemase genes were also identified in a small percentage of organisms, with specific prevalence varying among different species. Future molecular diagnostic panels could benefit from including additional ESBL targets beyond bla(CTX-M).
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Muhammad Hayat Haider, Timothy D. McHugh, Kerry Roulston, Lia Barbara Arruda, Zahra Sadouki, Saba Riaz
Summary: This study aimed to investigate the drug resistance of pathogens and develop a rapid diagnosis method for multidrug-resistant Gram-negative bacterial infections. The results showed that Pseudomonas aeruginosa is the main pathogen causing burn infections, and there are limited effective antibiotics available to combat such pathogens.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Immunology
Kwang Gon Kim, Jin Jung, Ji Hyun Shin, Hye Jung Park, Min Ji Kim, Jin Jong Seo, Young Ok Kim, Su-Ya Lee, Chang-Yee Cho, Tae Sun Kim
Summary: Due to differences in perspectives on human rights and laws in various social strata in Asian countries, the development of human rights laws in the Asian region has been relatively slow. In some countries in Asia, reform in the field of human rights laws has been hindered by government restrictions and pressures. The importance of human rights in Asian countries has gradually been recognized, but still faces many challenges.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Efthymia Protonotariou, Georgios Meletis, Dimitra Papadopoulou, Melania Kachrimanidou, Lilian Toptsi, Lemonia Skoura
Summary: The study compared the performance of AMR Direct Flow Chip Kit and phenotypic screening assays in dual specimens upon ICU admission, demonstrating that the AMR Direct Flow Chip Kit is a useful alternative for rapid detection of resistance markers.
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2021)
Article
Infectious Diseases
Vera Mijac, Snezana Brkic, Marija Milic, Marina Siljic, Valentina Cirkovic, Vladimir Perovic, Milos Markovic, Ivana Cirkovic, Maja Stanojevic
Summary: Our aim was to investigate the prevalence of carbapenem-resistant Enterobacterales (CRE) in preterm neonates at discharge from a Serbian tertiary care center. The study included 350 randomly selected neonates/infants discharged between April 2018 and May 2019. The results showed a high rate of CRE colonization, mainly attributed to carbapenem consumption and lack of screening as an infection prevention practice.
Article
Multidisciplinary Sciences
Temenuga Stoeva, Rumiana Markovska, Ivan Mitov
Summary: This study evaluated the epidemiological relationship between carbapenem-resistant (CR) and/or 3rd generation cephalosporin resistant Klebsiella pneumoniae blood isolates collected from patients in the University Hospital in Varna city during the period January 2014 - December 2017. The study also aimed to characterize the ESBLs and carbapenemase production in these isolates. The findings revealed a wide intrahospital dissemination of KPC-2 and/or CTX-M-15 producing Klebsiella pneumoniae, with ST15 persisting throughout the study period, demonstrating high cross transmission, epidemic and invasive potential.
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Hong-hao Huang, Yi-xing Lu, Su-juan Wu, Zhen-bao Ma, Dong-ping Zeng, Zhen-ling Zeng
Summary: Carbapenem- and colistin-resistant Enterobacter strains harboring blaIMI were isolated from intestinal samples and the environment of a duck farm in China. The study demonstrated the diversity of carbapenemases in a duck farm and provided evidence for the transmission of bla(IMI) genes. Whole-genome sequencing and comparative genetic analysis revealed the presence of chromosomal and plasmid bla(IMI) and identified the horizontal transmission of bla(IMI)-carrying plasmids.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Editorial Material
Microbiology
Ziad Daoud, Jean-Marc Rolain
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Immunology
Milena Dropa, Ziad Daoud
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Letter
Public, Environmental & Occupational Health
Iman Dandachi, Waleed Aljabr
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Editorial Material
Microbiology
Ziad Daoud
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Lina Reslan, Nour Youssef, Celina F. Boutros, Aia Assaf-Casals, Danielle Fayad, Sarah Khafaja, Fata Akl, Marc Finianos, Amena A. Rizk, Rouba Shaker, Alissar Zaghlout, Mireille Lteif, Bassam El Hafi, Mohammad Bahij Moumneh, Rita Feghali, Soha Ghanem, Tamima Jisr, Gilbert Karayakoupoglou, Malak Naboulsi, Monzer Hamze, Salam Samad, Elie Khoury, Ricardo Sarraf, Marwan Osman, Elie Bou Raad, Hadi El Amin, Ibrahim Abadi, Hicham Abdo, Marwan Chedid, Fatima Chamseddine, Angelique Barakat, Mohammad Houmani, Antoine Haddad, Georges Abdel Nour, Jacques E. Mokhbat, Ziad Daoud, Mohamad El-Zaatari, Elie Salem Sokhn, Nada Ghosn, Walid Ammar, Randa Hamadeh, Ghassan M. Matar, George F. Araj, Ghassan S. Dbaibo
Summary: The study found that the prevalence of PCV7 serotypes significantly decreased in the PCV13 era, while PCV13-only serotypes remained stable. Non-vaccine types (NVT) increased, especially serotypes 24 and 16F. Despite a substantial increase in mortality driven by NVT, a significant decrease in antimicrobial resistance (AMR) was observed, indicating a significant impact of PCVs on IPD and AMR in vaccinated and unvaccinated populations.
EXPERT REVIEW OF VACCINES
(2022)
Article
Immunology
Khaled El Khatib, Ribal Aby Hadeer, Anis Saad, Aline Kalaydjian, Elie Fayad, Youssef Mahfouz, Victorien Dougnon, Ziad Daoud, Roula M. Abdel-Massih
Summary: This study investigated the antibacterial activity of Ilex paraguariensis extract against different strains of nontyphoidal Salmonella. The extract showed varying MIC values and a high MPC, resulting in a wide MSW range. Several pathogenicity genes were detected in the Salmonella isolates. The antibacterial activity of the extract was not affected by antimicrobial resistance or pathogenicity genes, suggesting the presence of other active compounds.
MICROBIAL PATHOGENESIS
(2023)
Article
Oncology
Abdullah E. Al-Anazi, Bader S. Alanazi, Huda M. Alshanbari, Emad Masuadi, Maaweya E. Hamed, Iman Dandachi, Abdulrahman Alkathiri, Atif Hanif, Islam Nour, Hanadi Fatani, Hadel Alsaran, Fahad AlKhareeb, Ali Al Zahrani, Abdullah A. Alsharm, Saleh Eifan, Bandar Alosaimi
Summary: This study retrospectively collected demographic and clinical data of 146 nasopharyngeal carcinoma (NPC) patients and found a high prevalence (96%) of Epstein Barr Virus (EBV) infection, with genotype I being the most common. EBV infection was associated with lung metastasis but not with metastasis to other sites. The majority of patients were in advanced stages of cancer, and the overall survival mean time was 5.59 years. Further multi-center studies with larger sample sizes are needed to assess the true burden of EBV-associated NPC.
Article
Infectious Diseases
Majd Haddam, Mohammad Othman, Iman Dandachi, Ziad Daoud, Roula M. Abdel-Massih
Summary: This study aimed to determine the antibacterial effect of commercial diet pills on the intestinal microbiota. The results showed significant inhibition of the growth of intestinal microbiota by the diet pills. Further research is needed to understand the specific effects of the digested components on the intestinal microflora and human health.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2023)
Editorial Material
Microbiology
Ziad Daoud, Jean-Marc Rolain
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Rola Husni, Maroun Bou Zerdan, Nadia Samaha, Mariana Helou, Youssef Mahfouz, Rim Saniour, Sawsan Hourani, Harout Kolanjian, Claude Afif, Eid Azar, Tamima El Jisr, Jacques Mokhbat, Emma Abboud, Rita Feghali, Edmond Abboud, Hiam Matta, Gilbert Karayakouboglo, Madonna Matar, Rima Moghnieh, Ziad Daoud
Summary: This study describes the epidemiology of non-albicans Candida (NAC) infections and their resistance in Lebanese hospitals. The most common NAC species isolated were Candida glabrata (40.8%) and Candida tropicalis (23.1%). The study also found differing susceptibility to antifungal treatments among these species. Proper identification of these organisms is crucial for effective treatment.
FRONTIERS IN PUBLIC HEALTH
(2023)
Review
Microbiology
Mahdi Fadlallah, Ahmad Salman, Elie Salem-Sokhn
Summary: Carbapenem-resistant Enterobacterales (CRE) pathogens have been increasingly isolated and reported in Lebanon. However, compared to the worldwide data, research on CRE in the country is scarce and mostly limited to single center studies. This review aims to provide a comprehensive and reliable report on the current situation of CRE in Lebanon. Studies have shown a rising trend of carbapenem resistance in Enterobacterales since the first reports of CRE isolates in 2007 and 2008, with Escherichia coli and Klebsiella pneumoniae being the most frequently detected species. OXA-48 class D carbapenemases were the most prevalent among CRE isolates, but the emergence of other carbapenemases like NDM class B carbapenemase has also been observed. Strict infection control measures, including the identification of CRE carriers, are needed in Lebanese hospitals to prevent the spread of CRE. The dissemination of CRE in the community is attributed to various causes such as the refugee crisis, water contamination, and misuse of antimicrobials. In conclusion, strict infection control measures and antimicrobial stewardship programs are urgently needed in Lebanese healthcare settings.
INTERNATIONAL JOURNAL OF MICROBIOLOGY
(2023)
Article
Infectious Diseases
Mahdi Fadlallah, Elie Salem Sokhn
Summary: This study revealed the predominance of E. coli among Enterobacterales in Lebanese patients, with the urinary tract being the most common site of infection, and highlighted the high rates of antimicrobial resistance (AMR) in Enterobacterales in Lebanon.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2023)
Article
Infectious Diseases
Mariana Helou, Ahmad Mahdi, Ziad Daoud, Jacques Mokhbat, Anna Farra, Elma Nassar, Ralph Nehme, Edmond Abboud, Khalil Masri, Rola Husni
Summary: The epidemiology of CARTIs in Lebanon is important but understudied. This study presents the first Lebanese data on CARTI pathogens, revealing that viruses are the most common etiologies. Therefore, a different approach is needed for empirical management of CARTI, and rapid testing may help identify patients who require antibiotic therapy.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
