Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH

Absence of SARS-CoV-2 RNA in COVID-19-associated intestinal endothelialitis

(2021) Klaus Stahl et al.   INTENSIVE CARE MEDICINE

Circuits between infected macrophages and T cells in SARS-CoV-2 pneumonia

(2021) Rogan A. Grant et al.   NATURE

SARS-CoV-2 Infects Endothelial Cells In Vivo and In Vitro

(2021) Fengming Liu et al.   Frontiers in Cellular and Infection Microbiology

The Architecture of SARS-CoV-2 Transcriptome

(2020) Dongwan Kim et al.   CELL

Host-Viral Infection Maps Reveal Signatures of Severe COVID-19 Patients

(2020) Pierre Bost et al.   CELL

Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency

(2020) Calvin J. Gordon et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

and Animal Models for SARS-CoV-2 research

(2020) Kazuo Takayama   TRENDS IN PHARMACOLOGICAL SCIENCES

Tropism, replication competence, and innate immune responses of the coronavirus SARS-CoV-2 in human respiratory tract and conjunctiva: an analysis in ex-vivo and in-vitro cultures

(2020) Kenrie P Y Hui et al.   Lancet Respiratory Medicine

Coronavirus membrane fusion mechanism offers a potential target for antiviral development

(2020) Tiffany Tang et al.   ANTIVIRAL RESEARCH

Role of the early secretory pathway in SARS-CoV-2 infection

(2020) Daria Sicari et al.   JOURNAL OF CELL BIOLOGY

SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology

(2020) Natacha S. Ogando et al.   JOURNAL OF GENERAL VIROLOGY

Histopathology and ultrastructural findings of fatal COVID-19 infections in Washington State: a case series

(2020) Benjamin T Bradley et al.   LANCET

A molecular pore spans the double membrane of the coronavirus replication organelle

(2020) Georg Wolff et al.   SCIENCE

A unifying structural and functional model of the coronavirus replication organelle: Tracking down RNA synthesis

(2020) Eric J. Snijder et al.   PLOS BIOLOGY

β-Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway

(2020) Sourish Ghosh et al.   CELL

Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies

(2020) Mirko Cortese et al.   Cell Host & Microbe

Clustering and reverse transcription of HIV‐1 genomes in nuclear niches of macrophages

(2020) Elena Rensen et al.   EMBO JOURNAL

Cellpose: a generalist algorithm for cellular segmentation

(2020) Carsen Stringer et al.   NATURE METHODS

SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography

(2020) Steffen Klein et al.   Nature Communications

Human Coronavirus: Host-Pathogen Interaction

(2019) To Sing Fung et al.   Annual Review of Microbiology

ImJoy: an open-source computational platform for the deep learning era

(2019) Wei Ouyang et al.   NATURE METHODS

Visualization of Arenavirus RNA Species in Individual Cells by Single-Molecule Fluorescence In Situ Hybridization Suggests a Model of Cyclical Infection and Clearance during Persistence

(2018) Benjamin R. King et al.   JOURNAL OF VIROLOGY

Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation

(2018) Tomohiro Yamazaki et al.   MOLECULAR CELL

A Growing Toolbox to Image Gene Expression in Single Cells: Sensitive Approaches for Demanding Challenges

(2018) Xavier Pichon et al.   MOLECULAR CELL

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

(2017) Arnaud Hubstenberger et al.   MOLECULAR CELL

FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry

(2017) Riccardo Arrigucci et al.   Nature Protocols

Open-source do-it-yourself multi-color fluorescence smartphone microscopy

(2017) Yulung Sung et al.   Biomedical Optics Express

Nanoscale imaging of RNA with expansion microscopy

(2016) Fei Chen et al.   NATURE METHODS

smiFISH and FISH-quant – a flexible single RNA detection approach with super-resolution capability

(2016) Nikolay Tsanov et al.   NUCLEIC ACIDS RESEARCH

Multiplexed detection of viral infections using rapid in situ RNA analysis on a chip

(2015) Sydney M. Shaffer et al.   LAB ON A CHIP

Foldscope: Origami-Based Paper Microscope

(2014) James S. Cybulski et al.   PLoS One

FISH-quant: automatic counting of transcripts in 3D FISH images

(2013) Florian Mueller et al.   NATURE METHODS

Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization

(2013) Samuel O Skinner et al.   Nature Protocols

Single-mRNA counting using fluorescent in situ hybridization in budding yeast

(2012) Tatjana Trcek et al.   Nature Protocols

Validating transcripts with probes and imaging technology

(2011) Shalev Itzkovitz et al.   NATURE METHODS

Design of 240,000 orthogonal 25mer DNA barcode probes

(2009) Q. Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Imaging individual mRNA molecules using multiple singly labeled probes

(2008) Arjun Raj et al.   NATURE METHODS