Article
Multidisciplinary Sciences
Zhaohao Huang, Binyao Chen, Xiuxing Liu, He Li, Lihui Xie, Yuehan Gao, Runping Duan, Zhaohuai Li, Jian Zhang, Yingfeng Zheng, Wenru Su
Summary: Sex and aging have different effects on the human immune system, with females showing more plasma cells and a stronger immune response, while males have more NK cells and higher expression of inflammatory genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Developmental Biology
Jessica M. Toothaker, Oluwabunmi Olaloye, Blake T. McCourt, Collin C. McCourt, Tatiana N. Silva, Rebecca M. Case, Peng Liu, Dean Yimlamai, George Tseng, Liza Konnikova
Summary: Maintenance of a healthy pregnancy depends on the balance between the fetal and maternal immune systems. This study used advanced techniques to investigate the immune system in mid-gestation human placental villi and found multiple subtypes of fetal immune cells, including memory T cells. It was also discovered that T cells isolated from placental villi had enhanced proliferative capacity compared to adult T cells.
Article
Immunology
Songang Wu, Xuewen Li, Fan Hong, Qiang Chen, Yingying Yu, Shuanghui Guo, Yuanyuan Xie, Naian Xiao, Xuwen Kong, Wei Mo, Zhanxiang Wang, Shaoxuan Chen, Feng Zeng
Summary: This study used machine learning-based method to integrate public scRNA-seq datasets and established a comprehensive atlas of immune cells infiltrating in cross-age GBM. The findings showed remarkable differences in immune cell compositions across ages, with a significant increase of extracranial monocyte-derived macrophages (MDMs) in senior patients, which contributes to worse prognosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Feiyang Wang, Wentong Jia, Mengjie Fan, Xuan Shao, Zhilang Li, Yongjie Liu, Yeling Ma, Yu-Xia Li, Rong Li, Qiang Tu, Yan-Ling Wang
Summary: This study compared the cellular and molecular characteristics of decidual and peripheral leukocytes in normal and unexplained recurrent miscarriage (RM) pregnancies using single-cell RNA sequencing (scRNA-seq). The results revealed significant differences in immune cell subsets and molecular properties in RM cases, providing insights into the immune pathogenesis of early pregnancy loss.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Article
Immunology
Xinqiang Li, Shipeng Li, Bin Wu, Qingguo Xu, Dahong Teng, Tongwang Yang, Yandong Sun, Yang Zhao, Tianxiang Li, Dan Liu, Shuang Yang, Weihua Gong, Jinzhen Cai
Summary: This study used single-cell RNA sequencing to reveal the diversity of immune cells in liver transplantation, finding significant increases in memory CD4+ T cells and actively proliferating CD8+ T cells after transplantation, showing heterogeneity, distinct function, and homeostatic proliferation in liver graft.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Lingli Wang, Keilah G. Netto, Lujia Zhou, Xiaojie Liu, Ming Wang, Guojun Zhang, Paul S. Foster, Fuguang Li, Ming Yang
Summary: The study identified key immune cells and pathways involved in asthma exacerbation by establishing a mouse model of LPS-induced steroid-resistant exacerbation on the background of house dust mite-induced asthma. This provides important mechanistic insights for further understanding immune responses during asthma exacerbation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Xuan Li, Jingjun Lyu, Ran Li, Vaibhav Jain, Yuntian Shen, Angela del Aguila, Ulrike Hoffmann, Huaxin Sheng, Wei Yang
Summary: This study utilized single-cell RNA sequencing and a new transient ischemic stroke mouse model to generate, for the first time, a composite picture of immune cell populations in the aged brain at single-cell resolution post-stroke. At least 6 microglial subsets and major infiltrated myeloid cell subpopulations were identified with unique molecular profiles in the stroke aged brain.
JOURNAL OF NEUROINFLAMMATION
(2022)
Review
Virology
Tianyu Lei, Xiaohui Li, Fuhao Wang, Qingyu Huang, Tianxing Liu, Chao Liu, Qinyong Hu
Summary: Viral infections can lead to immune responses that trigger tumorigenesis and progression of cancer. To understand and target these responses, single-cell sequencing approaches have been used to analyze the tumor immune microenvironment (TIME). This review summarizes recent findings from sequencing studies on the immune infiltrate and antitumor response in virus-associated cancers, as well as the advancements in immunotherapy for these cancers. The strategic use of single-cell sequencing is expected to enhance our understanding of the TIME in viral cancers and lead to the development of more effective treatments.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chao Feng, Yujia Tao, Chao Yu, Lirui Wang, Xiao Liu, Yuan Cao
Summary: The tumor immune microenvironment (TIME) in anaplastic thyroid carcinoma (ATC) is a complex and diverse ecosystem. This study reveals the dynamic changes of TIME in both papillary thyroid carcinoma (PTC) and ATC at the single-cell level. The findings demonstrate the presence of immunosuppressive factors and decreased immune cell infiltration in ATC, providing insights for targeted therapy to activate the anti-tumor immune response.
CANCER GENE THERAPY
(2023)
Review
Chemistry, Multidisciplinary
Haiyang Xie, Xianting Ding
Summary: The advancement of single-cell protein analysis technologies enables high-throughput and multiplexed protein measurements, providing a more comprehensive exploration of cellular signaling processes in combination with RNA sequencing and mass spectrometry. While the potential applications are vast, there are still limitations and challenges to be addressed in this field.
Article
Immunology
Yi Bai, Dapeng Chen, Chuanliang Cheng, Zhongmin Li, Hao Chi, Yuliang Zhang, Xiaoyu Zhang, Shaohai Tang, Qiang Zhao, Bing Ang, Yamin Zhang
Summary: The study examined the tumor immune microenvironment in hepatocellular carcinoma (HCC) and discovered the specific distribution of regulatory T (Treg) cells in HCC. Immune checkpoints and metabolic pathways were found to be uniquely overexpressed in Treg cells. Additionally, two subsets of NK cells with different cytotoxic capacities and a unique state of memory B cells were identified in HCC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Alan T. Yeo, Shruti Rawal, Bethany Delcuze, Anthos Christofides, Agata Atayde, Laura Strauss, Leonora Balaj, Vaughn A. Rogers, Erik J. Uhlmann, Hemant Varma, Bob S. Carter, Vassiliki A. Boussiotis, Al Charest
Summary: Using single-cell transcriptomics and flow cytometry, this study investigated the changes in immune cell composition during the progression of glioblastoma (GBM). The results revealed a transition from pro-inflammatory microglia to infiltrating macrophages and suppressor cells as the tumor advanced.
Article
Immunology
Weiwei Deng, Zhen Su, Panpan Liang, Yubo Ma, Yufang Liu, Kai Zhang, Yi Zhang, Tianyu Liang, Jin Shao, Xiao Liu, Wenling Han, Ruoyu Li
Summary: The study revealed the expression patterns of immune checkpoints after Candida albicans infection by reanalyzing single-cell RNA-sequencing data, showing different immune pathway activities among cell subpopulations post-infection, indicating the importance of immune checkpoints in fungal infections.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Genetics & Heredity
Jiajing Lin, Li Liu, Fengque Zheng, Saiqiong Chen, Weiwei Yang, Jingjing Li, Steven Mo, Ding-Yuan Zeng
Summary: In this study, a global single-cell map of adenomyosis was constructed using single-cell sequencing data, and cell clusters with immune-related features were identified. The study explored the biological functions and evolutionary processes of these cell clusters. Additionally, a gene regulatory network analysis revealed the regulatory role of transcription factors in the progression of adenomyosis.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Zihao Mi, Zhenzhen Wang, Xiaotong Xue, Tingting Liu, Chuan Wang, Lele Sun, Gongqi Yu, Yuan Zhang, Peidian Shi, Yonghu Sun, Yongliang Yang, Shanshan Ma, Zhe Wang, Yueqian Yu, Jianjun Liu, Hong Liu, Furen Zhang
Summary: This study revealed the upregulation of APOE and exhaustion of CD8(+) T cells in L-LEP lesions through single-cell RNA sequencing. In addition, a remarkable enhancement of inhibitory immune receptors in L-LEP lesions was observed.
Article
Cell Biology
He Li, Lei Zhu, Rong Wang, Lihui Xie, Jie Ren, Shuai Ma, Weiqi Zhang, Xiuxing Liu, Zhaohao Huang, Binyao Chen, Zhaohuai Li, Huyi Feng, Guang-Hui Liu, Si Wang, Jing Qu, Wenru Su
Summary: Aging-induced changes in the immune system are associated with a higher incidence of infection and vaccination failure. In old mice, immune cell responses, especially Th17 cells, are altered, leading to a milder autoimmune response compared to young mice. This study highlights the role of GM-CSF secretion and IL-23R levels in regulating autoimmune challenges in aged mice.
Correction
Pharmacology & Pharmacy
Qi Jiang, Zhaohuai Li, Tianyu Tao, Runping Duan, Xianggui Wang, Wenru Su
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biology
Wen Shi, Jinguo Ye, Zhuoxing Shi, Caineng Pan, Qikai Zhang, Yuheng Lin, Yuanting Luo, Wenru Su, Yingfeng Zheng, Yizhi Liu
Summary: Using single-cell analysis, this study reveals transcriptomic and epigenomic changes in patients with the autoimmune disorder Vogt-Koyanagi-Harada disease (VKH), showing highly activated and pro-inflammatory immune cells, particularly the conventional dendritic cells (cDCs) that play a crucial role in inflammation regulation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Runping Duan, Lihui Xie, He Li, Rong Wang, Xiuxing Liu, Tianyu Tao, Shizhao Yang, Yuehan Gao, Xianchai Lin, Wenru Su
Summary: The study provides a comprehensive single-cell transcriptional atlas of immune cells under CsA therapy, revealing the reverse effects of CsA on cell composition, genomic expression, cell trajectory, and cell-cell communication. It was found that CsA reverses the changes in disease-related immune cell proportions, regulates key pathogenic factors, and may enhance the immunosuppressive function of Tregs. CsA also has an impact on plasma B cells differentiation and immunoglobulin secretion. Comparisons with glucocorticoids suggest that CsA may have a more premium rescue effect in attenuating the pathogenicity of autoreactive T cells.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Ophthalmology
Bingying Lin, Yangyang Li, Nan Jiang, Siyu Huang, Wenru Su, Yehong Zhuo
Summary: The study found that IL-35 can reduce retinal damage, decrease RGC death, and thinning of the IPL in retinal I/R. It also demonstrated the significant attenuation of pyroptosis in the retina. IL-35 was shown to have an inhibitory effect on pyroptosis in microglial cells and could directly suppress primary RGC apoptosis induced by OGD/R.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jingyi Peng, Jiayi Jin, Wenru Su, Wanwen Shao, Weihua Li, Zhiquan Li, Huan Yu, Yongxin Zheng, Liuxueying Zhong
Summary: Traumatic optic neuropathy (TON) is a significant cause of vision loss and irreversible blindness worldwide. The study demonstrates that HMGB1 mediates NLRP3 inflammasome via NF-kB to participate in retinal inflammatory injury after ONC. Intravitreal injection of BoxA has the potential therapeutic benefits for the effective treatment of RGC death to prevent TON.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Ophthalmology
Zhenzhen Liu, Shan Huang, Yingfeng Zheng, Tian Zhou, Leyi Hu, Lang Xiong, David Wan-cheng Li, Yizhi Liu
Summary: The crystalline lens is a transparent and refractive biconvex structure formed by lens epithelial cells (LECs) and lens fibers. Lens opacity, also known as cataracts, is the leading cause of blindness in the world. LECs are the principal cells of lens throughout human life, exhibiting different physiological properties and functions.
PROGRESS IN RETINAL AND EYE RESEARCH
(2023)
Article
Medicine, Research & Experimental
Xiuxing Liu, Qi Jiang, Jianjie Lv, Shizhao Yang, Zhaohao Huang, Runping Duan, Tianyu Tao, Zhaohuai Li, Rong Ju, Yingfeng Zheng, Wenru Su
Summary: In this study, we conducted a multimodal, high-dimensional immune atlas analysis using single-cell RNA sequencing and mass cytometry to investigate VKH patients undergoing TOFA therapy. The study revealed polarization of T cells, accumulation of monocytes, upregulation of cytokines and JAK/STAT signaling pathways in VKH patients. TOFA reversed the Th17/Treg imbalance, alleviated VKH symptoms, and reduced inflammation-related responses and intercellular interactions. Furthermore, the study identified two monocyte subpopulations strongly associated with VKH pathogenesis and mechanisms of TOFA treatment.
Article
Clinical Neurology
Jue-heng Pan, Shuo-long Wu, Jing-xiang Ma, Long Chang, Ying-feng Zheng, Xiao-dong Wang
Summary: Using data from the National Health and Nutrition Examination Survey (NHANES), the study found a reverse J-shaped association between circulating 25(OH)D and stroke history, with the lowest stroke prevalence observed at 60 nmol/L. The prevalence of stroke increased below and above the middle quintile of 25(OH)D (53.2-65.4 nmol/L). More high-quality evidence is needed to establish the causal relationship between vitamin D and stroke risk and the impact of vitamin D supplements on stroke prevention.
FRONTIERS IN NEUROLOGY
(2023)
Article
Immunology
Rong Wang, Lei Zhu, He Li, Xuening Peng, Sichen Zhao, Wenru Su
Summary: Mycophenolate mofetil (MMF), an immunosuppressive agent, has a general inhibitory effect on the activation of immune cells and can reverse some transcriptional changes induced by autoimmune uveitis (EAU). It can also inhibit the differentiation and pathogenic cytokine production of T and B cells, as well as the antigen processing and presentation ability of B cells. The therapeutic effect of MMF is driven by multiple molecules rather than a single one.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Zhuo-Xing Shi, Zhi-Chao Chen, Jia-Yong Zhong, Kun-Hua Hu, Ying-Feng Zheng, Ying Chen, Shang-Qian Xie, Xiao-Chen Bo, Feng Luo, Chong Tang, Chuan-Le Xiao, Yi-Zhi Liu
Summary: Long-read single-cell RNA isoform sequencing can reveal the complex landscape of alternative RNA splicing in individual cells, but it has a low read throughput. The authors developed circular consensus sequencing methods to achieve high-throughput and high-accuracy single-cell RNA isoform sequencing.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Xiuxing Liu, Yuhan Su, Zhaohao Huang, Jianjie Lv, Chenyang Gu, Zhuang Li, Tianyu Tao, Yidan Liu, Qi Jiang, Runping Duan, Binyao Chen, Rong Ju, Xianggui Wang, Yingfeng Zheng, Wenru Su
Summary: Sleep loss is associated with the higher risk of autoimmune and inflammatory disorders. The study found that sleep deprivation led to compositional and functional changes in immune cells, especially in effector CD4(+) T and myeloid cells. Sleep loss aggravated autoimmune uveitis development by promoting Th17 differentiation and activation.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Ophthalmology
Zitian Liu, Wenyong Huang, Zhenyu Wang, Ling Jin, Nathan Congdon, Yingfeng Zheng, Shida Chen, Yizhi Liu
Summary: This study demonstrates that the portable, self-imaging OCT is highly consistent with clinic-based SD-OCT in measuring CST and identifying most retinal lesions.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Letter
Ophthalmology
Tianyu Tao, Daquan He, Xuening Peng, Zhaohao Huang, Wenru Su
Summary: This study retrospectively followed up two BU patients with a history of BS for 2 and 10 years, respectively, and found that upadacitinib successfully treated BU, improving visual acuity, controlling intraocular inflammation, and resolving macular edema in both patients. One female patient showed mildly abnormal blood test results and slight liver enzyme elevation after 6 months of upadacitinib treatment, but no serious adverse events were reported in either patient during follow-up. Upadacitinib may be an important future option for managing recurrent and recalcitrant cases of BU, especially in patients with chronic ocular inflammation and macular edema resistant to conventional therapies.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2023)
Article
Biology
Wen Shi, Jinguo Ye, Zhuoxing Shi, Caineng Pan, Qikai Zhang, Yuheng Lin, Dan Liang, Yizhi Liu, Xianchai Lin, Yingfeng Zheng
Summary: This study presents a multi-omic single-cell analysis of Behcet's disease (BD), revealing the epigenetic and transcriptional characteristics of BD peripheral blood. It uncovers cell-type-specific active and pro-inflammatory immunity in BD, and identifies potential TF regulators that contribute to chromatin accessibility and gene expression. The study expands our understanding of potential epigenomic immunopathology in BD.
COMMUNICATIONS BIOLOGY
(2023)