Towards CRISPR powered electrochemical sensing for smart diagnostics

Even though global health has been steadily improved, the global disease burden associated with communicable and non-communicable diseases extensively increased healthcare expenditure. The present COVID-19 pandemic scenario has again ascertained the importance of clinical diagnostics as a basis to make life-saving decisions. In this context, there is a need for developing next-generation integrated smart real-time responsive biosensors with high selectivity and sensitivity. The emergence of clustered regularly interspaced short palin-dromic repeats (CRISPR)/Cas biosensing systems has shown remarkable potential for developing next-generation bio-sensors. CRISPR/Cas integrated electrochemical biosensors (E-CRISPR) stands out with excellent properties. In this opin-ionated review, we illustrate the rapidly evolving applications for E-CRISPR-integrated detection systems towards bio-sensing and the future scope associated with E-CRISPR based diagnostics.

Automated summary

Despite improvements in global health, the burden of communicable and non-communicable diseases has led to increased healthcare expenditure. The COVID-19 pandemic has highlighted the importance of clinical diagnostics. Developing next-generation biosensors with high sensitivity and selectivity, such as CRISPR/Cas systems, shows great potential for future diagnostic applications.