Article
Biochemical Research Methods
Lijian Wang, Li Leng, Ran Ding, Pengfei Gong, Chang Liu, Ning Wang, Hui Li, Zhi-Qiang Du, Bohan Cheng
Summary: Genetic selection for meat production in broilers leads to excessive abdominal fat deposition, impacting feed conversion efficiency and reproduction performance. This study integrated transcriptome and proteome analyses to identify key genes and pathways related to fat deposition differences between fat and lean broiler lines. Down-regulated genes and proteins were enriched in fatty acid metabolism pathways, while up-regulated ones were enriched in lysosome pathways. Numerous key genes and proteins involved in fatty acid uptake, synthesis, and lipid accumulation were discovered.
JOURNAL OF PROTEOMICS
(2021)
Article
Endocrinology & Metabolism
Sebastian Moller Nguyen Heimburger, Bjorn Hoe, Chris Neumann Nielsen, Natasha Chidekel Bergman, Kirsa Skov-Jeppesen, Bolette Hartmann, Jens Juul Holst, Flemming Dela, Julie Overgaard, Joachim Storling, Tina Vilsboll, Thomas Fremming Dejgaard, Jesper Foged Havelund, Vladimir Gorshkov, Frank Kjeldsen, Nils Joakim Faergeman, Martin Ronn Madsen, Mikkel B. Christensen, Filip Krag Knop
Summary: In men with type 1 diabetes, a 6-day subcutaneous GIP infusion transiently decreased bone resorption and increased NEFA and beta-oxidation. Further, hepatic fat content and supraclavicular skin temperature were increased without affecting WAT transcriptomics, the circulating proteome, lipids, or inflammatory markers.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Medicine, Research & Experimental
Benedikt Dietz, Juergen Machann, Vaibhav Agrawal, Martin Heni, Patrick Schwab, Julia Dienes, Steffen Reichert, Andreas L. Birkenfeld, Hans-Ulrich Haering, Fritz Schick, Norbert Stefan, Andreas Fritsche, Hubert Preissl, Bernhard Schoelkopf, Stefan Bauer, Robert Wagner
Summary: The study indicated that diabetes can be detected through whole-body MRI without additional data, with visceral fat playing a crucial role in diabetes classification. The model identified a high-risk group with increased risk for future diabetes and microalbuminuria.
Article
Cell Biology
Ruben Garcia-Martin, Bruna Brasil Brandao, Thomas Thomou, Emrah Altindis, C. Ronald Kahn
Summary: Exosomes/small extracellular vesicles (sEVs) can mediate cell-to-cell communication through their miRNA and protein cargo. Each cell type has a unique sEV proteome, but there are also common markers. Specific markers of different cell types can predict the tissue of origin of serum sEVs, as demonstrated in a mouse model.
Article
Biology
He Zhang, Erdu Ren, Rongying Xu, Yong Su
Summary: This study investigated the effects of short-term intravenous sodium butyrate (SB) administration on the metabolism of muscle and adipose tissue in pigs. The results showed that systemic butyrate displayed discriminative metabolic regulation in muscle and adipose tissues, affecting amino acid metabolism pathways, glucose catabolism, and lipolysis. The findings support the role of butyrate in regulating metabolism and energy metabolism as a metabolite of bacterial fermentation.
Article
Nutrition & Dietetics
Yueqi Lu, Ying Shan, Liang Dai, Xiaosen Jiang, Congying Song, Bangwei Chen, Jingwen Zhang, Jing Li, Yue Zhang, Junjie Xu, Tao Li, Zuying Xiong, Yong Bai, Xiaoyan Huang
Summary: The study developed and validated prediction models for measuring visceral fat and muscle, aiming to distinguish the relationships between individual body composition parameters and adverse health outcomes. The models estimated the volumes of visceral adipose tissue, abdominal subcutaneous adipose tissue, and fat-free muscle in the thigh based on demographic and anthropometric data. The models showed moderate to good accuracy, precision, and cardiometabolic correlations compared to traditional measures of obesity.
CLINICAL NUTRITION
(2023)
Article
Food Science & Technology
Taiyong Yu, Xuekai Tian, Dong Li, Yulin He, Peiyu Yang, Ye Cheng, Xin Zhao, Jingchun Sun, Gongshe Yang
Summary: In this study, omics methods were used to analyze the differences in meat quality characteristics among Chinese local pig breeds. The results revealed differences in fatty acids, amino acids, gene expression, protein abundance, and metabolite accumulation. Furthermore, the Weighted genes co-expression network construction identified RapGEF1 as a key gene related to intramuscular fat (IMF) content. This study provides fundamental data and new insights into unraveling the mechanism of pig IMF content.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Gastroenterology & Hepatology
Benjamin Schroeder, Sabine Kahl, Michael Roden
Summary: Individuals with obesity or type 2 diabetes have an increased risk of developing non-alcoholic fatty liver disease, with altered adipose tissue function and hepatic mitochondrial dysfunction playing key roles in the pathogenesis. Genetic testing for high-risk individuals, particularly for the rs738409(G) SNP of the PNPLA3 gene, could improve future screening strategies.
LIVER INTERNATIONAL
(2021)
Article
Endocrinology & Metabolism
Colm Mcelwain, Samprikta Manna, Andrea Musumeci, Isaac Sylvester, Chloe Rouchon, Anne Marie O'Callaghan, Mustafa Abdalla Bakhit Ebad, Fergus Mccarthy, Cathal Mccarthy
Summary: This study finds that GDM-obese pregnancies are characterized by inadequate VAT remodeling and dysfunctional molecular signaling, which contribute to insulin resistance and hinder metabolic health.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Multidisciplinary Sciences
Jiehan Li, Christopher Jin, Stefan Gustafsson, Abhiram Rao, Martin Wabitsch, Chong Y. Park, Thomas Quertermous, Joshua W. Knowles, Ewa Bielczyk-Maczynska
Summary: Adipogenesis is the process of fat-specific progenitor cells differentiating into adipocytes that perform key metabolic functions of adipose tissue. This study provides a single-cell RNA-Sequencing dataset of adipogenic differentiation in 3T3-L1 and SGBS cells, allowing for comparative analysis and understanding of cell-to-cell variability in these widely used in vitro models.
Review
Biochemistry & Molecular Biology
Jorge Valencia-Ortega, Rebeca Gonzalez-Reynoso, Edgar G. Ramos-Martinez, Aldo Ferreira-Hermosillo, Maria I. Pena-Cano, Enrique Morales-avila, Renata Saucedo
Summary: Gestational diabetes mellitus (GDM) is a common metabolic disorder during pregnancy with significant impact on the health of both mother and newborn. Recent research has focused on the expression levels and circulating characteristics of adipokines in different tissues of GDM patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Antu Antony, Zeqin Lian, Xiaoyuan Dai Perrard, Jerry Perrard, Hua Liu, Aaron R. Cox, Pradip Saha, Lothar Hennighausen, Sean M. Hartig, Christie M. Ballantyne, Huaizhu Wu
Summary: This study investigated the role of Stat1 in obesity-induced MDC polarization and inflammation, using mice with specific knockout of Stat1 in MDCs. The results showed that Stat1 in MDCs plays a crucial role in promoting M1-like polarization, contributing to AT inflammation, insulin resistance, and metabolic dysfunctions in obese mice.
Article
Multidisciplinary Sciences
Mari Honda, Ayaka Tsuboi, Satomi Minato-Inokawa, Mika Takeuchi, Miki Kurata, Bin Wu, Tsutomu Kazumi, Keisuke Fukuo
Summary: Limited expandability of subcutaneous adipose tissue may be a characteristic of first-degree relatives of type 2 diabetes. This study found an association between family history of type 2 diabetes (FHD) and reduced subcutaneous fat mass in young Japanese women, suggesting impaired adipose tissue expandability.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Wei Wei, Chengwan Zha, Aiwen Jiang, Zhe Chao, Liming Hou, Honglin Liu, Ruihua Huang, Wangjun Wu
Summary: Skeletal muscle fiber types can affect pork quality parameters. This study identified key proteins regulating muscle fiber types in pigs using proteomics and transcriptome data, and explored potential regulatory mechanisms through functional enrichment and protein-protein interaction analysis.
Article
Biochemistry & Molecular Biology
Kazushi Yamamoto, Masashi Yamashita, Masataka Oda, Vindy Tjendana Tjhin, Hiroyuki Inagawa, Gen-Ichiro Soma
Summary: This study confirms that oral administration of lipopolysaccharide (LPS) does not worsen type 2 diabetes mellitus (T2DM) and investigates the possible mechanisms. Oral LPS administration for 8 weeks suppresses abnormal glucose tolerance, insulin resistance, and T2DM progression in KK/Ay mice. In addition, the expression of insulin signaling-related factors is upregulated in adipose tissues, where an increase in adiponectin expression is observed for the first time with oral LPS administration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Justine Chee, Louise Lanoue, Dave L. Clary, Kendall Higgins, Lynette Bower, Ann Flenniken, Ruolin Guo, David Adams, Fatima Bosch, Robert E. Braun, Steve D. M. Brown, H. -J. Genie Chin, Mary Dickinson, Chih-Wei Hsu, Michael Dobbie, Xiang Gao, Sanjeev Galande, Anne Grobler, Jason Heaney, Yann Herault, Martin Hrabe de Angelis, Fabio Mammano, Lauryl M. J. Nutter, Helen Parkinson, Chuan Qin, Toshi Shiroishi, Radislav Sedlacek, J-K Seong, Ying Xu, Brian Brooks, Colin McKerlie, K. C. Kent Lloyd, Henrik Westerberg, Ala Moshiri
Summary: This study identified new genes and pathways associated with eye development through screening of mouse genes. These findings provide insights into the molecular and cellular mechanisms of eye development and could potentially contribute to the diagnosis and treatment of congenital blinding diseases.
Article
Biochemistry & Molecular Biology
Grace Png, Raffaele Gerlini, Konstantinos Hatzikotoulas, Andrei Barysenka, N. William Rayner, Lucija Klaric, Birgit Rathkolb, Juan A. Aguilar-Pimentel, Jan Rozman, Helmut Fuchs, Valerie Gailus-Durner, Emmanouil Tsafantakis, Maria Karaleftheri, George Dedoussis, Claus Pietrzik, James F. Wilson, Martin Hrabe Angelis, Christoph Becker-Pauly, Arthur Gilly, Eleftheria Zeggini
Summary: In this study, a protein quantitative trait locus (pQTL) analysis was performed on two Greek cohorts, identifying 301 independently associated pQTL variants for 170 proteins related to cardiometabolic processes. The study also discovered rare variants and observed changes in frequency of certain pQTL variants in the isolated populations. Additionally, the study identified proteins causally associated with cardiometabolic traits and described a knock-out Mep1b mouse model.
HUMAN MOLECULAR GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Payel Sen, Bachuki Shashikadze, Florian Flenkenthaler, Esther Van de Kamp, Siyu Tian, Chen Meng, Michael Gigl, Thomas Froehlich, Daphne Merkus
Summary: Pulmonary vein stenosis (PVS) causes pulmonary hypertension (PH) by affecting flow and pressure in the pulmonary vasculature, leading to endothelial dysfunction and metabolic changes. Targeted therapy is recommended to relieve pressure and reverse flow-related changes in this type of PH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Bachuki Shashikadze, Sophie Franzmeier, Isabel Hofmann, Martin Kraetzl, Florian Flenkenthaler, Andreas Blutke, Thomas Froehlich, Eckhard Wolf, Arne Hinrichs
Summary: This study investigated the histomorphological and molecular alterations associated with growth hormone receptor deficiency (GHRD). The results showed that GHRD pigs had reduced body weights, decreased pituitary volumes, and reduced volume density of GH-storing granules in somatotroph cells. Proteomic analysis revealed changes in protein expression related to protein production, transport, and endoplasmic reticulum (ER) stress. These findings provide insights into the molecular and histological changes caused by GHRD.
JOURNAL OF NEUROENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Manuela A. Oestereicher, Janine M. Wotton, Shinya Ayabe, Ghina Bou About, Tsz Kwan Cheng, Jae-Hoon Choi, Dave Clary, Emily M. Dew, Lahcen Elfertak, Alain Guimond, Hamed Haseli Mashhadi, Jason D. Heaney, Lois Kelsey, Piia Keskivali-Bond, Federico Lopez Gomez, Susan Marschall, Michael McFarland, Hamid Meziane, Violeta Munoz Fuentes, Ki-Hoan Nam, Zuzana Nichtova, Dale Pimm, Lynette Bower, Jan Prochazka, Jan Rozman, Luis Santos, Michelle Stewart, Nobuhiko Tanaka, Christopher S. Ward, Amelia M. E. Willett, Robert Wilson, Robert E. Braun, Mary E. Dickinson, Ann M. Flenniken, Yann Herault, K. C. Kent Lloyd, Ann-Marie Mallon, Colin McKerlie, Stephen A. Murray, Lauryl M. J. Nutter, Radislav Sedlacek, Je Kyung Seong, Tania Sorg, Masaru Tamura, Sara Wells, Elida Schneltzer, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe de Angelis, Jacqueline K. White, Nadine Spielmann
Summary: This study provides the first reference ranges for electrocardiography in laboratory mice, which are important for diagnostic decision-making and pre-clinical scientific research.
Review
Biochemistry & Molecular Biology
Jiri Lindovsky, Zuzana Nichtova, Nathalia R. V. Dragano, David Pajuelo Reguera, Jan Prochazka, Helmut Fuchs, Susan Marschall, Valerie Gailus-Durner, Radislav Sedlacek, Martin Hrabe de Angelis, Jan Rozman, Nadine Spielmann
Summary: Cardiovascular diseases have a high mortality rate globally and pose a significant burden on health care systems. The International Mouse Phenotyping Consortium (IMPC) utilizes mouse models to study cardiovascular diseases and aims to target each protein-coding gene to examine multiple organ systems. This review discusses the recent advances of the IMPC in cardiac research, focusing on the diagnostic requirements for high-throughput electrocardiography and transthoracic echocardiography in mice. Additionally, it explores the relationship between metabolism and the heart, highlighting the phenotypes that emerge in knockout mice for certain genes and identifying novel loss-of-function genes affecting both metabolism and the cardiovascular system.
Article
Biochemistry & Molecular Biology
Christina Bukas, Isabella Galter, Patricia da Silva-Buttkus, Helmut Fuchs, Holger Maier, Valerie Gailus-Durner, Christian L. Mueller, Martin Hrabe de Angelis, Marie Piraud, Nadine Spielmann
Summary: Echo2Pheno is an automatic statistical learning workflow specifically designed for analyzing and interpreting high-throughput non-anesthetized transthoracic murine echocardiographic images. It accurately confirms known cardiovascular genotype-phenotype relationships and discovers novel genes causing altered cardiovascular phenotypes. This provides an important step toward automatic end-to-end learning for linking echocardiographic readouts to cardiovascular phenotypes in conscious mice.
Article
Biochemistry & Molecular Biology
Patricia da Silva-Buttkus, Nadine Spielmann, Tanja Klein-Rodewald, Christine Schuett, Antonio Aguilar-Pimentel, Oana V. Amarie, Lore Becker, Julia Calzada-Wack, Lillian Garrett, Raffaele Gerlini, Markus Kraiger, Stefanie Leuchtenberger, Manuela A. Oestereicher, Birgit Rathkolb, Adrian Sanz-Moreno, Claudia Stoeger, Sabine M. Hoelter, Claudia Seisenberger, Susan Marschall, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe de Angelis
Summary: Rare diseases pose challenges in medicine due to their diverse clinical manifestations and low prevalence. The lack of specific treatments for the majority of rare diseases highlights the need for research. Genome sequencing technology allows for the identification of potential disease-causing genes, but further confirmation is required. Mouse knockout models are crucial for studying the genetics of rare diseases and the German Mouse Clinic is a pioneer in this field. Collaboration between research institutions, clinicians, and patient groups is necessary to advance the understanding and treatment of rare diseases.
Article
Multidisciplinary Sciences
Ken Ikeuchi, Nives Ivic, Robert Buschauer, Jingdong Cheng, Thomas Froehlich, Yoshitaka Matsuo, Otto Berninghausen, Toshifumi Inada, Thomas Becker, Roland Beckmann
Summary: This study reveals the impact of Otu2-driven deubiquitylation of ribosomal protein eS7 on translational efficiency and provides insights into its role in translation reset. The authors found that eS7 in actively translating 80S ribosomes is monoubiquitinated by the E3 ligase Not4 and deubiquitinated by Otu2 upon ribosomal subunit recycling. Cryo-electron microscopy analysis showed that Otu2 mainly binds to the 40S subunit surface at sites not occupied by other factors.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Daniel Garger, Martin Meinel, Tamina Dietl, Christina Hillig, Natalie Garzorz-Stark, Kilian Eyerich, Martin Hrabe de Angelis, Stefanie Eyerich, Michael P. P. Menden
Summary: Through studying the association between various phenotypic traits and lifespan, it was found that somatic mutation and resting heart rate are negatively correlated with lifespan, while other traits show strong associations. Resting heart rate enhances the prediction of lifespan, indicating its direct influence or representation of lower-level mechanisms associated with lifespan.
Article
Environmental Sciences
Federica Lamberto, Bachuki Shashikadze, Radwa Elkhateib, Salvo Danilo Lombardo, Alex Horanszky, Andrea Balogh, Kornel Kistamas, Melinda Zana, Joerg Menche, Thomas Froehlich, Andras Dinnyes
Summary: This study investigated the effects of environmentally relevant doses of Bisphenol A (BPA) on developing cardiomyocytes using a human induced pluripotent stem cell (hiPSC)-derived model. The results showed that long-term treatment with low doses of BPA interferes with hiPSC-CMs functionality and alters the surrounding cellular environment, leading to potential diseases. This study contributes to the understanding of BPA effects on developing human foetal cardiomyocytes and provides a suitable model for environmental chemical hazard and risk assessment.
ENVIRONMENTAL POLLUTION
(2023)
Article
Multidisciplinary Sciences
Michael Stirm, Bachuki Shashikadze, Andreas Blutke, Elisabeth Kemter, Andreas Lange, Jan B. Stoeckl, Florian Jaudas, Laeticia Laane, Mayuko Kurome, Barbara Kessler, Valeri Zakhartchenko, Andrea Baehr, Nikolai Klymiuk, Hiroshi Nagashima, Maggie C. Walter, Wolfgang Wurst, Christian Kupatt, Thomas Froehlich, Eckhard Wolf
Summary: Skipping DMD exon 51 can restore dystrophin expression and improve cardiac function in DMD patients and animal models.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Developmental Biology
Pia Sessenhausen, Karolina M. Caban, Nicole Kreitmair, Mirko Peitzsch, Jan B. Stoeckl, Marie C. Meinsohn, David Pepin, Bastian Popper, Thomas Froehlich, Artur Mayerhofer
Summary: The nicotinic acetylcholine receptor alpha 7 (nAChRa7), encoded by Chrna7, is expressed in various ovarian cells and plays a role in the local regulation of the ovary.
Article
Endocrinology & Metabolism
Leona Kovac, Thomas Goj, Meriem Ouni, Martin Irmler, Markus Jaehnert, Johannes Beckers, Martin Hrabe De Angelis, Andreas Peter, Anja Moller, Andreas L. Birkenfeld, Cora Weigert, Annette Schuermann
Summary: Exercise training can improve glucose tolerance in obese individuals, but some may not respond positively. This study analyzed gene expression and DNA methylation in skeletal muscle of low and high responders to endurance training, identifying differences in gene expression and potential novel markers for intervention success.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Meeting Abstract
Endocrinology & Metabolism
R. Jumpertz von Schwartzenberg, K. Bodis, J. Martin, M. Schon, M. Hrabe de Angelis, N. Perakakis, S. Kabisch, A. Pfeiffer, M. Blueher, J. Szendroedi, N. Stefan, R. Wagner, A. L. Birkenfeld, M. Roden, A. Fritsche