期刊
METABOLITES
卷 12, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/metabo12010028
关键词
gill mucus; skin mucus; Atlantic salmon; biomarkers; non-invasive sampling; data normalization
资金
- Norwegian Veterinary Institute strategic program BIO-DIRECT: Biomarkers and bioassays for future research and diagnostics via Research Council of Norway
This study compared the metabolite profiles of plasma, skin, and gill mucus of freshwater Atlantic salmon using liquid chromatography coupled to high-resolution mass spectrometry. The targeted metabolomics data showed that mucus metabolite composition could be used for fish health monitoring and research.
Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon (Salmo salar) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据