Article
Cell Biology
Qingyu Zhang, Jun Dong, Peng Zhang, Dongsheng Zhou, Fanxiao Liu
Summary: Key gene regulatory events were identified in early stages of lineage commitment in BMSCs, with two transcription factors (Tsc22d3 and Epas1) showing elevated expression throughout different stages of three-directional differentiation in rats.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Wei Cao, Xiaohong Yang, Xiao Hua Hu, Jun Li, Jia Tian, RenJun OuYang, Xue Lin
Summary: This study analyzed the biological characteristics of the mandible and mandibular bone marrow mesenchymal stem cells in the bone microenvironment of postmenopausal osteoporosis. It identified miR-344d-3p as a promoter of osteogenic differentiation and inhibitor of adipogenic differentiation, with Dnmt3a potentially being its target gene. These findings are of significant importance in the field of bone reconstruction.
Article
Multidisciplinary Sciences
Matthew Prideaux, Christian S. Wright, Megan L. Noonan, Xin Yi, Erica L. Clinkenbeard, Elsa Mevel, Jonathan A. Wheeler, Sharon Byers, Asiri R. Wijenayaka, Stan Gronthos, Uma Sankar, Kenneth E. White, Gerald J. Atkins, William R. Thompson
Summary: Mesenchymal progenitor cells can differentiate into various tissues such as bone, cartilage, and adipose. The developed mesenchymal progenitor cell lines MPC1 and MPC2 can differentiate into different cell types under temperature control.
SCIENTIFIC REPORTS
(2021)
Review
Endocrinology & Metabolism
Fangyu An, Xiaxia Wang, Chunmei Wang, Ying Liu, Bai Sun, Jie Zhang, Peng Gao, Chunlu Yan
Summary: Osteoporosis (OP) is characterized by imbalanced bone homeostasis due to decreased osteoblasts and increased adipocytes in the bone marrow compartment. Bone marrow mesenchymal stem cells (BMSCs) can differentiate into osteoblasts and adipocytes to maintain bone homeostasis. Studies have shown that the lncRNA-miRNA network plays a crucial role in regulating the bone/fat differentiation of BMSCs, which is closely related to the treatment of OP and the development of novel therapies. However, there is a limited summary of the effects of the lncRNA-miRNA network on osteogenic/adipogenic differentiation in BMSCs. Therefore, this article aims to review the current literature and explore the impact of the lncRNA-miRNA network on the osteogenic/adipogenic differentiation of BMSCs, providing a new theoretical basis for the treatment of OP.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Chenglong Wang, Sabine Stoeckl, Shushan Li, Marietta Herrmann, Christoph Lukas, Yvonne Reinders, Albert Sickmann, Susanne Graessel
Summary: This study investigated the effects of extracellular vesicles (EVs) isolated from different stages of human bone marrow mesenchymal stem cells (hBMSCs) on the osteogenic and adipogenic differentiation capacity of naive hBMSCs. The results showed that EVs from late-stage osteogenic differentiated hBMSCs promoted osteogenic potential in naive hBMSCs and inhibited their adipogenic differentiation. Proteomic analysis revealed distinct protein profiles in EVs from osteogenic and naive hBMSCs.
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Chemistry, Applied
Na Ren, Xin Yu, Aizhu Wang, Na Liang, Zhichao Feng, Chunhui Sun
Summary: The study demonstrates that ScCl3 can enhance proliferation and osteogenic differentiation of rat bone MSCs while reducing adipogenic differentiation to a certain extent through the Wnt/beta-catenin signaling pathway. Appropriate concentrations of ScCl3 show potential in improving bone remodeling and may lead to new strategies for designing composites for biomedical applications in the future.
JOURNAL OF RARE EARTHS
(2022)
Article
Materials Science, Ceramics
Julietta V. Rau, Adriana De Stefanis, Katia Barbaro, Marco Fosca, Viktoriya G. Yankova, Roberto Matassa, Stefania Annarita Nottola, Qaisar Nawaz, Muhammad Saad Ali, Wolfgang Peukert, Aldo R. Boccaccini
Summary: An innovative Mn-containing glass ceramic was prepared by sol-gel method and characterized using various techniques. The study demonstrated that the material promoted stem cell differentiation and exhibited antimicrobial properties.
JOURNAL OF NON-CRYSTALLINE SOLIDS
(2021)
Article
Oncology
Yibo Xu, Zhimeng Wang, Yakang Wang, Qiang Huang, Cheng Ren, Liang Sun, Qian Wang, Ming Li, Hongliang Liu, Zhong Li, Kun Zhang, Teng Ma, Yao Lu
Summary: This research investigated the mechanism of autophagy in bone tissue regeneration by analyzing the differential gene expression. Several autophagy-related genes associated with osteogenesis were identified and their expression levels were validated through in vitro experiments.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2022)
Article
Environmental Sciences
Hui Qin, Yuxin Niu, Haiyang Luan, Minghan Li, Lu Zheng, Yifan Pan, Wei Liu
Summary: This study found that PFASs have different adverse effects and relative potency on the multipotent differentiation of human mesenchymal stem cells, depending on the integrated action of three PPAR subtypes. The results contribute to a better understanding of the molecular initiating events of PFASs, explain the health issues caused by PFASs, and emphasize the necessity of risk assessment for alternatives.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Xiu-qin Hu, Han Song, Na Li, Chun-xiang Hao, Bo Zhang, Xin-peng Li, Jie Xin, Yong-qing Zhang
Summary: We sequenced miRNAs from male and female flower buds of the dioecious plant Trichosanthes kirilowii Maxim. using Illumina high-throughput sequencing technology. Through bioinformatics analysis and association analysis with previous transcriptome sequencing data, we identified 80 differentially expressed miRNAs between female and male plants. We also predicted target genes for these miRNAs and established a regulatory network, identifying 12 core genes involved in sex differentiation in TK.
Article
Biotechnology & Applied Microbiology
Jialin Liu, Yuan Yao, Jinyong Huang, Hao Sun, Yixuan Pu, Mengting Tian, Meijie Zheng, Huiyu He, Zheng Li
Summary: This study investigated the expression profiles of lncRNAs, miRNAs, and mRNAs in osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). The study identified key molecules and pathways involved in this process, providing potential molecular targets for the management of bone defect.
Article
Engineering, Biomedical
Mengqi Shi, Song Wen, Boxin Zhang, Minni Liu, Zhang Yan, Wu Qun, Yumei Zhang
Summary: The study investigated the influence of nanotube dimensions on ER stress and osteogenic differentiation of cells. It was found that increasing nanotube dimensions led to morphological changes in the ER and up-regulation of ER stress-related genes.
BIOMEDICAL MATERIALS
(2021)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)