Article
Engineering, Environmental
Yulin Fu, Yiqiang Chen, Dejun Liu, Dawei Yang, Zhihai Liu, Yingyu Wang, Jiayi Wang, Xueyang Wang, Xiangyue Xu, Xing Li, Junjia He, Junyao Jiang, Weishuai Zhai, Lingli Huang, Tao He, Xi Xia, Chang Cai, Yang Wang, Haiyang Jiang
Summary: The study found that tet(X4), tmexCD1-toprJ1, and other tigecycline resistance genes were more abundant in chicken feces, indicating a higher risk of TRG propagation in chicken feces. Positive correlations between ISCR2/IS26 and TRGs further demonstrated the potential transfer and mobilization of these resistance genes.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Microbiology
Tian Deng, Yuqian Jia, Ziwen Tong, Jingru Shi, Zhiqiang Wang, Yuan Liu
Summary: Antibiotic resistance poses a serious threat to public health and human safety. The emergence of the novel resistance gene tet(X4) and its variants has jeopardized the clinical utility of tigecycline, a last-line antibiotic for multidrug-resistant bacterial infections. However, the combination of bismuth drugs, particularly bismuth nitrate [Bi(NO3)(3)], with tigecycline has shown to effectively enhance its antibacterial activity against tet(X)-positive bacteria. This is achieved by inhibiting the enzymatic activity of the Tet(X) protein through competitive and noncompetitive binding mechanisms.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jing Wang, Han Wu, Cai-Yue Mei, Yan Wang, Zhen-Yu Wang, Meng-Jun Lu, Zhi-Ming Pan, Xinan Jiao
Summary: Tigecycline-resistant Enterobacteriaceae strains isolated from a pig farm in Shanghai, China carried tet(X) and tet(A) resistance genes, which were transmitted via plasmids or chromosomal integrative and conjugative elements (ICEs). The rational use of antibiotics and surveillance of antibiotic resistance in livestock are crucial to combat the emergence and spread of tigecycline resistance.
MICROBIOLOGY SPECTRUM
(2021)
Article
Environmental Sciences
Shiting Dai, Dejun Liu, Ziming Han, Yang Wang, Xiaofei Lu, Min Yang, Yu Zhang
Summary: This study investigated the environmental behavior of mobile tet(X)-variant genes in manure composting treatments and amended soils. The results showed that tet(X4) was the most prevalent mobile tet(X)-variant gene, and it exhibited the lowest reduction after thermophilic composting, possibly due to its correlation with ISCR2.
Article
Microbiology
Yumeng Cheng, Yakun Li, Meng Yang, Yang He, Xinru Shi, Zhidan Zhang, Yesheng Zhong, Yuan Zhang, Hongbin Si
Summary: In this study, two MDR Acinetobacter indicus strains were analyzed and characterized. It was found that BDT201 carried three tet(X) variants, while BDT2076 carried multiple resistance genes. The continuous emergence of MDR bacteria and resistance genes is a global environmental health issue.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Yumeng Cheng, Yakun Li, Runhao Yu, Mingxiang Ma, Meng Yang, Hongbin Si
Summary: The emergence of the tet(X) gene poses a serious challenge to global public health security, as the resistance to clinical tigecycline continues to rise. This research identified seven novel tet(X3) variants from tigecycline-resistant Acinetobacter sp. strains in fecal samples from Chinese farms. The study suggests a risk of horizontal transfer of resistant genes and highlights the importance of continuous monitoring of tigecycline resistance.
MICROBIOLOGY SPECTRUM
(2022)
Review
Microbiology
Shaqiu Zhang, Jinfeng Wen, Yuwei Wang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Dekang Zhu, Xinxin Zhao, Ying Wu, Qiao Yang, Juan Huang, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Bin Tian, Anchun Cheng
Summary: With the widespread use of antibiotics, antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are considered as emerging environmental pollutants. The new tetracycline-class antibiotic, tigecycline, is now the last resort for treating multidrug-resistant bacteria. Plasmid-mediated horizontal transfer allows for genetic information sharing among different bacteria. However, the transmission of the tigecycline resistance gene tet(X) may pose a risk to public health and the concept of One Health.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Cuihong Tong, Danyu Xiao, Longfei Xie, Jintao Yang, Ruonan Zhao, Jie Hao, Zhipeng Huo, Zhenling Zeng, Wenguang Xiong
Summary: The study revealed a high richness and diversity of ARGs in swine manure, with inactivating tetracycline resistance genes being prevalent. Workers and the environment were identified as primary recipients of ARGs, with shared ARGs accounting for at least 90% of their ARG abundances. Network analysis showed Escherichia, Acinetobacter, and Erysipelothrix as the most dominant genera co-occurring with specific shared ARGs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Ruichao Li, Yan Li, Kai Peng, Yi Yin, Yuan Liu, Tao He, Li Bai, Zhiqiang Wang
Summary: This study identified 58 strains of bacteria carrying the tet(X4) gene in fresh pork samples from multiple regions in China, with Escherichia coli being the predominant species. Four novel tet(X4)-positive bacterial species were also characterized. Genetic analysis revealed various types of core tet(X4)-bearing genetic environments and tandem duplications. The study highlights the potential transmission risk of animal-borne tet(X4)-bearing pathogens in consumed food, emphasizing the urgent need for large-scale monitoring and effective control measures.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biotechnology & Applied Microbiology
Huseyin Hatipoglu, Gulay Erman, Hande Toptan, Mehmet Koroglu, Mustafa Altindis
Summary: This study compared the flow cytometry method with current standardized methods for determining the antibiotic susceptibility of bacterial strains. The results showed a high level of correlation between the two methods. Flow cytometry provided faster results within the same day and shortened the duration of the antibiotic susceptibility tests.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Infectious Diseases
Yu Zeng, Jiayue Lu, Congcong Liu, Zhuoren Ling, Qiaoling Sun, Hanyu Wang, Hongwei Zhou, Yanyan Hu, Gongxiang Chen, Rong Zhang
Summary: The study developed an effective enrichment method for tet(X) detection using PCR and Sanger sequencing. Results showed a 2.26% prevalence of tet(X4)-positive Escherichia coli, with 96.6% exhibiting resistance to tigecycline. This specific screening strategy will aid in advancing knowledge of tet(X) and tigecycline resistance mechanisms.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Yingshan Li, Kai Peng, Yi Yin, Xinran Sun, Wenhui Zhang, Ruichao Li, Zhiqiang Wang
Summary: The study identified widespread dissemination of different tet(X) variants in the chicken farming environment, with tet(X4) being the dominant variant. It is recommended to limit antibiotic usage to control the emergence and transmission of novel tet(X) variants in the poultry industry.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Shiting Dai, Qing He, Ziming Han, Wenli Shen, Ye Deng, Yang Wang, Wei Qiao, Min Yang, Yu Zhang
Summary: The epicPCR method is developed to accurately identify and locate bacterial hosts carrying the tet(X4) gene, revealing a more diverse range of bacteria involved in harboring tet(X4) than previously realized.
Article
Microbiology
Chong Chen, Chao-Yue Cui, Xiao-Ting Wu, Liang-Xing Fang, Qian He, Bing He, Teng-Fei Long, Xiao-Ping Liao, Liang Chen, Ya-Hong Liu, Jian Sun
Summary: The recent discovery of plasmid-mediated tigecycline-resistant gene tet(X) in sick ducks and chickens in Guangdong, China, challenges the clinical effectiveness of tigecycline. The isolates exhibited high degradation activities on tetracyclines by hydroxylation and showed multidrug resistance to several antibiotics. Additional efforts are needed to evaluate the clinical impact of these tigecycline resistance genes.
VETERINARY MICROBIOLOGY
(2021)
Article
Infectious Diseases
Kai Lucassen, Carina Mueller, Julia Wille, Kyriaki Xanthopoulou, Meredith Hackel, Harald Seifert, Paul G. Higgins
Summary: The most prevalent tigecycline resistance mechanisms in carbapenem-resistant Acinetobacter baumannii isolates were caused by alterations in the regulators of RND-type efflux pumps, with disruption of the adeN gene being the most common. Mutations and IS element disruptions in the adeRS regulatory system from Southern European countries were associated with higher tigecycline MICs, although less commonly observed.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Genetics & Heredity
Yuhan Li, Yan Li, Kefan Bu, Mianzhi Wang, Zhiqiang Wang, Ruichao Li
Summary: A total of 53 pork samples were collected from markets in Jiangsu, China, and 23 tet(X4)-positive isolates were identified, showing resistance to multiple antibiotics. This study systematically investigated the tet(X4)-positive isolates and emphasized the importance of continuous surveillance of tet(X4) in pork.
Article
Microbiology
Xi Lu, Yanxiang Wang, Wei Guo, Zhimeng Zhang, Xinxin Hu, Tongying Nie, Xinyi Yang, Congran Li, Xiukun Wang, Xue Li, Yun Lu, Guoqing Li, Youwen Zhang, Lang Sun, Jing Pang, Xuefu You
Summary: CEL, a natural product, exhibits strong antibacterial activity against VRE and restores the susceptibility of VRE to vancomycin. Furthermore, it binds and inhibits the bacterial cell-division protein FtsZ, showing promising potential as a therapeutic option in combating VRE.
MICROBIOLOGY SPECTRUM
(2023)
Article
Environmental Sciences
Yuqian Jia, Zeyu Wang, Shuyao Zhu, Zhiqiang Wang, Yuan Liu
Summary: The prevalence and spread of multidrug-resistant bacteria is a global challenge to public health. This study assessed the effect of certain disinfectants on bacterial transformation and found that these disinfectants significantly enhanced the transformation of plasmid-encoded antibiotic resistance genes. The study also revealed the mechanisms underlying the promotive effect of disinfectants on transformation. These findings provide new insights into the spread of antibiotic resistance genes through bacterial transformation and indicate potential risks associated with the widespread use of disinfectants.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Chemistry, Multidisciplinary
Xinyu Miao, Yinyan Yin, Yulian Chen, Wenhui Bi, Yuncong Yin, Sujuan Chen, Daxin Peng, Lizeng Gao, Tao Qin, Xiufan Liu
Summary: A novel multi-antiviral metastable iron sulfides (mFeS) has been developed to target various influenza A/B subtype viruses. mFeS induces high levels of lipid peroxidation and center dot OH free radicals in the viral envelope, leading to viral ferroptosis and the loss of viral infectibility and pathogenicity. The decoction of mFeS inhibits intracellular viral replication by correcting virus-induced reprogrammed sulfur metabolism. mFeS represents a broad-spectrum antiviral alternative with potential therapeutic effect against influenza virus.
Article
Microbiology
Tao He, Jun Li, Lan Gong, Yang Wang, Ruichao Li, Xing Ji, Fengting Luan, Minmin Tang, Lei Zhu, Ruicheng Wei, Ran Wang
Summary: This study found that heavy metals may play an important role in the transmission of transferable tigecycline resistance genes (tet(X3) and tet(X4)) within the animal manure-related environment in China. Heavy metals have a coselection effect on the tet(X3)/tet(X4) gene on the same plasmid and can increase the conjugation ability of tet(X3)/tet(X4)-carrying plasmids. The conjugation-promoting concentrations of heavy metals are lower than the maximal limits defined in the national standard for fertilizers, indicating a high transmission risk of tet(X3)/tet(X4) genes. These findings will provide scientific evidence for the future development of effective measures to reduce antimicrobial resistance dissemination.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yuncong Yin, Yuan Liu, Juan Fen, Kaituo Liu, Tao Qin, Sujuan Chen, Daxin Peng, Xiufan Liu
Summary: This study identifies the first evidence of the low-pathogenicity H7N9 avian influenza virus infecting camels, posing a high risk to public health.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yongjia Jiang, Kai Peng, Qiaojun Wang, Mianzhi Wang, Ruichao Li, Zhiqiang Wang
Summary: A novel trimethoprim resistance gene, dfrA49, was identified in Riemerella anatipestifer isolates from poultry farms in Jiangsu and Guangdong province. This study expands the knowledge of mobile dfrA genes that confer resistance to trimethoprim and provides insights into antibiotic resistance in R. anatipestifer.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Xiaobo Liu, Ruichao Li, Edward Wai-Chi Chan, Sheng Chen
Summary: Bacterial antimicrobial resistance, especially phenotypic resistance to multiple drugs (MDR), has posed a serious threat to public health worldwide. Understanding the mechanisms of transmission of multidrug resistance encoding plasmids in Enterobacterales is crucial for controlling the spread of MDR pathogens. In this study, plasmids in an Escherichia coli strain isolated from chicken meat samples were characterized, revealing the presence of multiple antimicrobial resistance genes and the potential for transmission of resistance determinants.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Xiaoyu Lu, Wenhui Zhang, Mashkoor Mohsin, Mianzhi Wang, Jingui Li, Zhiqiang Wang, Ruichao Li
Summary: The prevalence and molecular characterization of mcr-1-positive isolates in pigeons close to humans were investigated after the ban on the use of colistin as an animal feed additive in China. It was found that mcr-1-positive E. coli isolates from pigeons were mainly mediated by IncX4 plasmids. However, different transferability and fitness of mcr-1-bearing IncX4 plasmids in E. coli were observed, suggesting the influence of chromosomal factors in the same bacterial species on plasmid transferability.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Xiaoyu Lu, Pei Zhang, Pengcheng Du, Xiuli Zhang, Juan Wang, Yingying Yang, Honghu Sun, Zhiqiang Wang, Shenghui Cui, Ruichao Li, Li Bai
Summary: This study investigated the prevalence and characteristics of mcr-harboring Escherichia coli strains in China. It identified 135 mcr-1-harboring E. coli isolates from various sources including humans, animals, and foods. Multiple resistance genes were found in these isolates, and the study demonstrated the role of different plasmids in the spread of mcr-1.
MICROBIOLOGY SPECTRUM
(2023)
Review
Environmental Sciences
Shuyao Zhu, Bingqing Yang, Zhiqiang Wang, Yuan Liu
Summary: The emergence and rapid spread of antibiotic resistance seriously compromise the clinical efficacy of current antibiotic therapies, representing a serious public health threat worldwide. Generally, drug-susceptible bacteria can acquire antibiotic resistance through genetic mutation or gene transfer. However, recent evidence suggests that non-antibiotics can also accelerate the horizontal transfer of antibiotic resistance genes, but their role and mechanism are largely underestimated.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Infectious Diseases
Xiaoyu Lu, Lifei Zhang, Kai Peng, Qiaojun Wang, Ruoqi Liu, Zhiqiang Wang, Ruichao Li
Summary: This study characterized a novel tigecycline resistance gene tet(X22) and its coexistence with carbapenem resistance gene blaNDM-1 in Pseudomonas caeni. The study found that tet(X22) confers resistance to tigecycline and may widely spread to different hosts. Continuous monitoring of the global spread of pathogens harboring tet(X) and blaNDM variants is necessary according to the One Health approach.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Hui Yang, Yurui Dong, Ying Bian, Chenzhi Huo, Chuncheng Zhu, Tao Qin, Sujuan Chen, Daxin Peng, Xiufan Liu
Summary: The H5 subtype avian influenza virus (AIV) PA protein suppresses host antiviral defense by degrading the key protein JAK1 involved in interferon signaling. Mutations in the PA protein contribute to the pathogenicity of the virus in both avian and mammalian hosts. These findings provide insights into the molecular mechanisms of virus-host interactions and potential therapeutic targets for controlling avian influenza.