Review
Chemistry, Multidisciplinary
Lihua Ding, Xiaonan Yang, Zibo Gao, Clement Yaw Effah, Xiaoju Zhang, Yongjun Wu, Lingbo Qu
Summary: Exosomes are small extracellular vesicles secreted by various cells into body fluids and hold great potential for disease diagnosis and therapy. However, isolating exosomes is challenging due to their heterogeneity and the complexity of bio-fluids. Recent advancements in microfluidic technologies offer an efficient system for exosome extraction, showcasing high speeds, portability, and low sample volumes.
Article
Biophysics
Zixian Yu, Shujing Lin, Fangfang Xia, Yanlei Liu, Di Zhang, Fei Wang, Yanpu Wang, Qichao Li, Jiaqi Niu, Chengxi Cao, Daxiang Cui, Nengquan Sheng, Jiazi Ren, Zhigang Wang, Di Chen
Summary: The ExoSD chip is a highly integrated platform for exosome separation and detection, capable of efficiently separating and detecting gastric cancer cell-derived exosomes with high recovery and purity.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Mehdi Mohammadi, Hossein Zargartalebi, Razieh Salahandish, Raied Aburashed, Kar Wey Yong, Amir Sanati-Nezhad
Summary: This study provides an overview of the role of exosomes in cancer diagnosis and prognosis, investigates emerging exosome technologies, evaluates the advantages and drawbacks of different technologies, and discusses their clinical challenges for cancer diagnosis and prognosis. Commercial platforms for exosomal detection and analysis are introduced, with a focus on their performance and impact on cancer diagnosis and prognosis, as well as the risks associated with the development of next-generation exosome devices.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biology
Adem Ozcelik, Ozge Cevik
Summary: Exosomes in bodily fluids are important biomarkers for clinical diagnosis, and microfluidics-based methods have been used to isolate and purify exosomes. These methods can be divided into passive and active isolation mechanisms, employing inertial and hydrodynamic forces or external forcefields, respectively. This paper discusses the application of microfluidic methods in exosome isolation and highlights future perspectives in this field.
Article
Chemistry, Analytical
Premanshu Kumar Singh, Aarti Patel, Anastasia Kaffenes, Catherine Hord, Delaney Kesterson, Shaurya Prakash
Summary: This article provides a tutorial review of the existing microfluidic technologies for cancer diagnostics, with a focus on extracellular vesicle isolation methods. Extracellular vesicles have generated significant interest as biomarker cargo holders for cancer diagnosis. However, the isolation and purification of these vesicles remain challenging. Technological advances in microfluidics have the potential to overcome these challenges and enable point-of-care diagnostics for cancer.
Article
Materials Science, Multidisciplinary
Sajad Razavi Bazaz, Sareh Zhand, Robert Salomon, Elham Hosseini Beheshti, Dayong Jin, Majid Ebrahimi Warkiani
Summary: This study used the ImmunoInertial microfluidics approach to isolate specific subpopulations of cancer-specific small extracellular vesicles (sEVs), demonstrating high efficiency and selectivity. The method has the advantages of high sensitivity, low cost, and ability to process samples of varying volumes, making it a promising strategy for cancer diagnosis and prognosis in liquid biopsies.
APPLIED MATERIALS TODAY
(2023)
Review
Environmental Sciences
Esmail Pishbin, Fatemeh Sadri, Amin Dehghan, Mohammad Javad Kiani, Nader Hashemi, Iman Zare, Pegah Mousavi, Amid Rahi
Summary: Alzheimer's disease, a progressive neurological condition, requires early diagnosis for effective treatment management. This paper reviews recent advancements in detecting Alzheimer's disease related exosomal microRNAs using different methods such as electrochemical, fluorescent, and SPR. The use of microfluidic devices to isolate and detect these valuable biomarkers is also summarized. Challenges with the performance of novel technologies for isolating and detecting exosomal microRNAs are addressed.
ENVIRONMENTAL RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Ilgin Kimiz-Gebologlu, Suphi S. Oncel
Summary: This review discusses the general properties and biological functions of exosomes, as well as their potential in drug delivery systems. It provides an overview of methods for large-scale production, isolation, and drug loading of exosomes, and investigates the current challenges and potential directions in this emerging field of drug delivery.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Analytical
Zixuan Zhou, Yan Chen, Xiang Qian
Summary: In this paper, a method for rapid and efficient isolation of exosomes by targeting exosome-carrying CD63 and PTK 7 in a microfluidics system using modified microchannels and aptamers is proposed.
Article
Chemistry, Analytical
Natasha Ramnauth, Elise Neubarth, Amy Makler-Disatham, Mazhar Sher, Steven Soini, Vivian Merk, Waseem Asghar
Summary: This study presents a double filtration microfluidic device that can rapidly isolate exosomes via size-exclusion principles in point-of-care settings. The device efficiently isolates exosomes from plasma samples within 50 minutes and exhibits earlier miRNA detection compared to a polyethylene glycol-based method. Comparative analysis of exosomes collected from different pore sizes of membrane filters shows increased sample purity. The developed microfluidic device is cost-efficient and time-efficient, making it ideal for cancer and disease diagnostics and therapeutics in low-resourced and point-of-care settings.
Article
Biochemistry & Molecular Biology
Huiying Fang, Mei Liu, Wei Jiang
Summary: Breast cancer cell-derived exosomes have the potential to be used as biomarkers for continuous biopsies and monitoring in breast cancer. A flexible microfluidic chip with a three-dimensional structure of interlaced cylinders doped with nickel powder was designed for exosome separation. The chip achieved high exosome recovery rate (> 74%) and purity (> 67%) in continuous flow mode, making it a cutting-edge platform for exosome separation and detection.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Sun Jiawei, Chen Zhi, Tian Kewei, Li Xiaoping
Summary: In this study, commonly used exosome isolation approaches are reviewed, with a focus on magnetic bead-based capture, which offers high purity and integrity of exosomes. The challenges of traditional methods for exosome isolation are also described, highlighting the directions for future research and development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Navya Rastogi, Pranjal Seth, Ramray Bhat, Prosenjit Sen
Summary: The novel orthogonal vortex chip enables size-based cell separation at lower velocities to avoid cell damage caused by high flow speeds. The device utilizes shear-gradient lift, centrifugal, and Dean flow drag forces to selectively trap particles of different sizes, showing promising results for gentle isolation of rare cells from blood.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Biomedical
Xiaoni Fang, Yuqing Wang, Shurong Wang, Baohong Liu
Summary: Exosomes have attracted significant research interest as a new paradigm of liquid biopsy. However, the efficient isolation and sensitive molecular quantification of exosomes is still a major obstacle, and nanomaterials have been identified as powerful tools for effective purification and molecular analysis of exosomes.
MATERIALS TODAY BIO
(2022)
Review
Chemistry, Analytical
Krishan Kumar, Eunsu Kim, Munirah Alhammadi, Umapathi Reddicherla, Sheik Aliya, Jitendra N. Tiwari, Hyun Sung Park, Jung Hyun Choi, Chae Yeon Son, A. T. Ezhil Vilian, Young-Kyu Han, Jiyoon Bu, Yun Suk Huh
Summary: This paper provides a comprehensive overview of microfluidic devices used to isolate, detect, and analyze exosomes from body fluids, with an emphasis on their designs and working principles from an analytical perspective. Recent progress in the design of microfluidic-based exosome isolation devices is outlined. Challenges and potentials in the clinical translation of these devices are highlighted, and guidelines are offered to engineers and clinicians interested in this field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xiaoli Liu, Huichao Lin, Jiaao Song, Taiyi Zhang, Xiaoying Wang, Xiaowen Huang, Chengyun Zheng
Summary: Cell culture is crucial for screening anti-cancer drug candidates, with traditional 2D culture being widely used. 3D cellular spheroids offer a better mimic of in vivo microenvironment. The SimpleDrop method provides a user-friendly approach for 3D spheroid formation and drug analysis, showing potential for cell-cell interaction research, tissue engineering, and drug screening.
Article
Physics, Applied
Lijuan Hou, Limin Zhang, Jinhao Zang, Weixia Shen, Taiyi Zhang, Xiaowen Huang, Huiyu Yuan, Dezhi Kong, Ye Wang, Xinjian Li, Tingting Xu
Summary: K0.8Ti1.73Li0.27O4 (KLTO) is an important titanium-based anode material for lithium-ion batteries (LIBs) that could replace carbonaceous materials due to its nontoxicity, low cost, and high safety performance. A Fe-doped KLTO@rGO (Fe-KLTO@rGO) composite was prepared and exhibited excellent high-rate and long-cycle-life performance as an anode in LIBs. The Fe-KLTO@rGO composite sample showed high specific capacity and stability.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2022)
Review
Chemistry, Analytical
Weidu Song, Taiyi Zhang, Huichao Lin, Yujing Yang, Gaozhen Zhao, Xiaowen Huang
Summary: This paper introduces the crucial role of nucleic acid testing (NAT) in containing the spread of SARS-CoV-2 and provides a comprehensive review and comparison of various detection technologies, including traditional techniques and microfluidic-based technologies, discussing their characteristics and development prospects.
Review
Chemistry, Analytical
Taiyi Zhang, Feng Ding, Yujing Yang, Gaozhen Zhao, Chuanhao Zhang, Ruiming Wang, Xiaowen Huang
Summary: In vitro diagnosis (IVD) is a hot topic in laboratory research and achievement transformation. Microfluidic paper-based analytical devices (μPADs) have played a significant role in advancing the development of IVD. This paper provides a comprehensive review of μPAD classification and research progress in IVD, and discusses the potential improvements in terms of portability, sensitivity, and automation.
Article
Chemistry, Multidisciplinary
Peilong Li, Jiaci Chen, Yuqing Chen, Shangling Song, Xiaowen Huang, Yang Yang, Yanru Li, Yao Tong, Yan Xie, Juan Li, Shunxiang Li, Jiayi Wang, Kun Qian, Chuanxin Wang, Lutao Du
Summary: In this study, a 3D porous sponge microfluidic chip with an exosome enrichment platform was constructed, achieving a capture efficiency of approximately 90%. A specific exosome membrane protein (SORL1) for colorectal cancer was identified through deep mass spectrometry analysis, and a specific quantum dot labeling method for SORL1 detection was developed. An ensemble classification system was established using features extracted from 64-patched fluorescence images, and it showed a significantly higher area under the curve (AUC) of 0.99 compared to the conventional biomarker carcinoembryonic antigen (CEA) with an AUC of 0.71. This system demonstrated similar diagnostic performance for early-stage CRC, young CRC, and CEA-negative CRC patients.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Ziyu Huang, Lei Wang, Chonghui Yang, Jiaci Chen, Gaozhen Zhao, Xiaowen Huang
Summary: With the increasing concern over the energy crisis, solar energy has been recognized as a green and unlimited source. This paper introduces a microreactor for photocatalytic reactions, which efficiently converts solar energy into chemical energy. By developing a fully immobilized microreactor (FIM) and a controllable partially immobilized microreactor (PIM), the demands for low cost, high transparency, and easy fabrication are met. The microreactor demonstrated high efficiency in coenzyme regeneration and valuable product synthesis, indicating its great potential in future photocatalytic applications.
Article
Chemistry, Physical
Huichao Lin, Yang Liu, Chonghui Yang, Gaozhen Zhao, Jiaao Song, Taiyi Zhang, Xiaowen Huang
Summary: Artificial photosynthesis is an effective method to convert solar energy into chemical energy, and it plays an important role in the regeneration of coenzymes and synthesis of organic matter. However, traditional photocatalytic materials and oxidoreductases have problems such as poor stability and inability to be reused. This study presents an efficient and stable microfluidic artificial photosynthetic system for reduced nicotinamide adenine dinucleotide (NADH) regeneration and l-glutamate synthesis, with advantages of high efficiency, low cost, and sustainability.
CATALYSIS SCIENCE & TECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Limin Zhang, Dezhi Kong, Qianqian Zhuang, Minglang Wang, Taiyi Zhang, Jinhao Zang, Weixia Shen, Tingting Xu, Di Wu, Yongtao Tian, Ye Wang, Xinjian Li, Xiaowen Huang
Summary: In this study, large-area layer-tunable 1T-phase PtSe2 films with rich active edges were fabricated on carbon cloth, demonstrating excellent performance as an electrocatalyst for the hydrogen evolution reaction in acidic media. The vertically aligned layer structures of 1T-PtSe2 combined with a highly conductive CC substrate ensured high electron transfer efficiency, resulting in low overpotential and favorable Tafel slope for HER. These findings suggest that the large-scale growth of edge-rich 1T-PtSe2 holds great promise for high-performance HER applications.
JOURNAL OF MATERIALS CHEMISTRY C
(2021)