Article
Chemistry, Multidisciplinary
Yong Wang, Xiaoxu Chen, Wenkang Cai, Linzhi Tan, Yutong Yu, Boyang Han, Yuxuan Li, Yuanzhe Xie, Yeyu Su, Xiaozhou Luo, Tao Liu
Summary: Incorporation of structurally novel noncanonical amino acids (ncAAs) into proteins was achieved through biosynthesis, with nearly 50 ncAAs synthesized from simple small-molecule precursors. Furthermore, bioorthogonal reactive groups could be incorporated into proteins with high yields for subsequent chemical conjugation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Environmental Sciences
Xiao-Dong Pei, Fan Li, Shi-Yang Yue, Xiao-Ni Huang, Tian-Tian Gao, Dao-Quan Jiao, Cheng-Hua Wang
Summary: This study reported the isolation of a highly efficient feather-degrading Pseudomonas aeruginosa 4-3, which produces keratinase with high activity, thermal stability, and organic solvent tolerance. The keratinase from P. aeruginosa 4-3 efficiently degrades feathers, and the strain also produces aminopeptidases with similar characteristics, resulting in a high degradation rate of feathers when both enzymes are used together.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemical Research Methods
Changgeng Xu, Qin Zou, Jiheng Tian, Mengyuan Li, Baowen Xing, Julia Gong, Jiangyun Wang, Yi-Xin Huo, Shuyuan Guo
Summary: The UAG-based genetic code expansion (GCE) allows the incorporation of noncanonical amino acids (ncAAs) with novel functionalities into target proteins. However, most studies on GCE have been limited to a few extensively engineered chassis cells to avoid the presence of UAG stop codons. This limits the application of GCE in other microbial cell factories, such as Bacillus subtilis. In this study, researchers engineered a strain of B. subtilis, named Bs-22, to minimize the negative impact of GCE on the expression of essential genes. The study demonstrated efficient overexpression of ncAA-rich proteins in Bs-22, shedding light on the trade-off between efficiency and workload.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biochemical Research Methods
Jessica T. Stieglitz, Kelly A. Potts, James A. Van Deventer
Summary: The study introduces single plasmid systems (SPSs) for genetic code expansion in yeast and compares their noncanonical amino acid (ncAA) incorporation capabilities with previously described dual plasmid systems (DPSs). Results show that SPSs perform equally well as DPSs in terms of ncAA incorporation efficiency, making them a convenient alternative. This suggests that SPSs can be used for high-throughput investigations on genetic or genomic changes affecting ncAA incorporation efficiency and the eukaryotic translation apparatus.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhiyang Hu, Jinming Liang, Taogeng Su, Di Zhang, Hao Li, Xiangdong Gao, Wenbin Yao, Xiaoda Song
Summary: By analyzing the gene sequences of giant virus species, differences at the tRNA binding interface between MjTyrRS and MVTyrRS were identified. The size of the anticodon-recognized loop of MjTyrRS was found to affect its suppression activity, leading to the design of loop-minimized MjTyrRS mutants. These mutants showed increased suppression activity and enhanced incorporation of ncAAs, suggesting the potential of loop minimization as a strategy for efficient synthesis of ncAA-containing proteins.
Article
Energy & Fuels
Nolene Ramalingum, Prashant Bhagwat, Kugenthiren Permaul, Santhosh Pillai
Summary: The production of keratinase from Pseudomonas aeruginosa S-04, which efficiently degrades feathers, was studied. Optimization of various parameters led to a 1.3-fold improvement in enzyme production. The purified keratinase displayed optimal activity at 60°C and pH 9.5, with stability in a range of temperatures and pH levels. The enzyme's activity was enhanced by certain ions and solvents.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemistry & Molecular Biology
Jichao Wang, Ruliang Pi, Guangwen Lu
Summary: This study presents the high-resolution structure of the insulin-cleaving membrane protease (ICMP) from Pseudomonas aeruginosa, revealing an atypical HxxE motif and a V-shaped cleft responsible for coordinating a metal ion. The structure provides insights into potential substrate-binding and catalytic sites, paving the way for future studies to elucidate the mechanism of ICMP catalysis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Moritz Pott, Matthias Tinzl, Takahiro Hayashi, Yusuke Ota, Daniel Dunkelmann, Peer R. E. Mittl, Donald Hilvert
Summary: Changing the primary metal coordination sphere is an effective strategy for tuning metalloprotein properties, as demonstrated in this study. The variants with different histidine analogues showed distinct activities, impacting cyclopropanation, N-H insertion, and S-H insertion. The genetically encoded analogues provide valuable tools for exploring mechanisms and enabling new chemistries in enzymes with functional histidine residues.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Joshua Trae Hampton, Chia-Chuan Dean Cho, Demonta D. Coleman, Zhi Zachary Geng, Peng-Hsun Chase Chen, Gopal K. Dubey, Lauralee D. Sylvain, Shiqing Xu, Wenshe Ray Liu
Summary: Using an amber suppression-based ncAA mutagenesis approach, a novel helper phage, CMa13ile40, was developed to enrich amber obligate phage clones and produce ncAA-containing phages efficiently. CMa13ile40 allowed for continuous amber codon enrichment for two different libraries with a 100-fold increase in packaging selectivity. Two peptide libraries containing separate ncAAs were created using CMa13ile40 and used to identify peptide ligands with low micromolar affinity for ZNRF3 dependent on the ncAA used.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemical Research Methods
Delilah Jewel, Rachel E. E. Kelemen, Rachel L. L. Huang, Zeyu Zhu, Bharathi Sundaresh, Xiaofu Cao, Kaitlin Malley, Zeyi Huang, Muhammad Pasha, Jon Anthony, Tim van Opijnen, Abhishek Chatterjee
Summary: Virus-assisted directed evolution of tRNAs (VADER) allows for the efficient enrichment of active and orthogonal tRNA mutants from naive libraries in mammalian cells. Using this method, improved mutants of Methanosarcina mazei pyrrolysyl-tRNA and a bacterial tyrosyl-tRNA were obtained. The most efficient mutant pyrrolysyl-tRNA showed specific activity for mammalian cells, suggesting improved interaction with the unique mammalian translation machinery.
Article
Microbiology
Huawei Yi, Jing Zhang, Famin Ke, Xiurong Guo, Jian Yang, Peijuan Xie, Li Liu, Qin Wang, Xiaowei Gao
Summary: Incorporation of noncanonical amino acids (ncAAs) into proteins allows for manipulation of protein structure and function, with potential applications in industrial settings. Studying the transcriptome and proteome profiles of E. coli C321.Delta A, a strain ideal for ncAA-containing protein expression, revealed differential expression of metabolic pathways and functions compared to the parental strain. Integration of the T7 RNA polymerase system further improved yields of ncAA-containing proteins in this strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Vladyslava Liudkovska, Pawel S. Krawczyk, Aleksandra Brouze, Natalia Guminska, Tomasz Wegierski, Dominik Cysewski, Zuzanna Mackiewicz, Jonathan J. Ewbank, Krzysztof Drabikowski, Seweryn Mroczek, Andrzej Dziembowski
Summary: This study reveals that cytoplasmic polyadenylation is a previously unknown component of the posttranscriptional regulation of innate immunity in animals, enhancing the expression of secreted innate immunity effector proteins.
Article
Biotechnology & Applied Microbiology
Ke Liu, Ling Jiang, Shuang Ma, Zhongdi Song, Lun Wang, Qunfeng Zhang, Renhao Xu, Lirong Yang, Jianping Wu, Haoran Yu
Summary: This study constructed a highly active and substrate promiscuous variant of PylRS, which successfully incorporated multiple noncanonical amino acids into proteins. The study further elucidated the impact of these noncanonical amino acids on the catalytic mechanism of enzymes.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Chemistry, Multidisciplinary
Arianna O. Osgood, Yunan Zheng, Soumya Jyoti Singha Roy, Nikolaos Biris, Myer Hussain, Conor Loynd, Delilah Jewel, James S. Italia, Abhishek Chatterjee
Summary: Site-specific incorporation of multiple distinct noncanonical amino acids (ncAAs) into proteins in mammalian cells is a promising technology, where each ncAA must be assigned to a different orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pair that reads a distinct nonsense codon. Here we show that the E. coli tryptophanyl (EcTrp) pair is an excellent TGA-suppressor in mammalian cells, which can be combined with other established pairs to develop new routes for dual-ncAA incorporation. This study demonstrates the efficient site-specific incorporation of multiple ncAAs into proteins, expanding the scope of this technology.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Hidetoshi Teramoto, Katsura Kojima, Masatoshi Iga, Taiyo Yoshioka
Summary: The translational incorporation of a synthetic amino acid, 3-azidotyrosine (3-AzTyr), into Bombyx mori silk fiber can improve its material properties, such as mechanical strength and solubility, without affecting its crystalline structure. This finding suggests that the incorporation of synthetic amino acids is an efficient method to enhance the properties of silk-based materials.
Article
Chemistry, Organic
Xuedan Wu, Qin Wang, Diane Dickie, Lin Pu
JOURNAL OF ORGANIC CHEMISTRY
(2020)
Article
Chemistry, Medicinal
Wan-Li Tan, Chun Zhang, Yang Li, Kai Guo, Xiao-Wei Gao, Jun Wei, Dong Yi, Lin Pu, Qin Wang
Summary: In this study, 40 optically active falcarindiol analogues were synthesized and their anticancer activities on various cell lines were evaluated. The results indicated that the falcarindiol analogue (3R,8S)-2 i with a terminal double bond exhibited the most potent anti-proliferation effect on Hccc-9810 cells, inducing apoptosis through increased LDH release and MDA content, and decreased SOD activity, leading to oxidative stress accumulation.
Article
Plant Sciences
Yang Li, Wan-Li Tan, Kai Guo, Xiao-Wei Gao, Jun Wei, Dong Yi, Chun Zhang, Qin Wang
Summary: Falcarinol analogues (R)-2d and (R)-2i have shown neuroprotective effects on PC12 cells injured by NaN3, possibly through protecting against oxidative stress. Additionally, both falcarinol (1a) and its analogue (R)-2i exhibited potential L-type calcium channel-blocking activity, suggesting a possible mechanism for neuroprotection involving inhibition of calcium overload.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Microbiology
Huawei Yi, Jing Zhang, Famin Ke, Xiurong Guo, Jian Yang, Peijuan Xie, Li Liu, Qin Wang, Xiaowei Gao
Summary: Incorporation of noncanonical amino acids (ncAAs) into proteins allows for manipulation of protein structure and function, with potential applications in industrial settings. Studying the transcriptome and proteome profiles of E. coli C321.Delta A, a strain ideal for ncAA-containing protein expression, revealed differential expression of metabolic pathways and functions compared to the parental strain. Integration of the T7 RNA polymerase system further improved yields of ncAA-containing proteins in this strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Famin Ke, Peijuan Xie, Yanrong Yang, Liu Yan, Ailing Guo, Jian Yang, Jing Zhang, Li Liu, Qin Wang, Xiaowei Gao
Summary: Research on the effects of AMPs and herbs on the gut microbiome of farmed fish suggests that these substances can alter the richness, diversity, and composition of the intestinal microbiota, with potential anti-inflammatory effects and enhancement of digestive enzyme activities in the fish intestine.
FRONTIERS IN NUTRITION
(2021)
Article
Chemistry, Multidisciplinary
Jia-Yi Li, Li Wang, Li-Ping Yin, Xin-Meng Jiang, Kai Guo, Chun Zhang, Shan-Shan Yu, Xiao-Qi Yu, Qin Wang
Summary: A new racemic naphthyl-coumarin-based probe was discovered to covalently bind with amino acids, generating distinct circular dichroism (CD) signals. These strong CD signals allowed for quantitative enantiomeric excess analysis of amino acids and enantioselective sensing of amines and amino alcohols. The reaction mechanism of the coumarin-aldehyde probe with amino acids was investigated through CD, UV-Vis, NMR, ESI-MS analysis, and ECD calculation.
Article
Microbiology
Lijuan Wu, Lisha Ran, Yazeng Wu, Manyu Liang, Jing Zeng, Famin Ke, Fang Wang, Jian Yang, Xiaoqing Lao, Li Liu, Qin Wang, Xiaowei Gao
Summary: Studies have shown that 5-HTP can improve depressive symptoms in mice by modulating the gut microbiome and partially restoring its diversity and richness. It also exhibits positive effects on the concentrations of short-chain fatty acids and brain-derived neurotrophic factors altered by depression.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yan Ding, Shengjiao Yu, Man Ren, Ji Lu, Qiang Fu, Zhijie Zhang, Qin Wang, Jun Bai, Na Hao, Lin Yang, Siping Wei, Dong Yi, Jun Wei
Summary: This paper describes a metal- and aldehyde-free visible-light-driven photoredox-neutral alkene acylarylation using readily available cyanoarenes. A variety of 3-(arylmethyl)chroman-4-ones (homoisoflavonoids) and analogs are efficiently synthesized with good functional group tolerance. This mild protocol relies on a phosphoranyl radical-mediated acyl radical-initiated cyclization and selective radical-radical coupling sequence, and is also further highlighted by subsequent derivatization to chromone and 2H-chromene as well as its application in the three-component alkene acylarylation.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Manyu Liang, Jing Zhang, Yanhong Yang, Yi Xia, Lintao Liu, Li Liu, Qin Wang, Xiaowei Gao
Summary: Nattokinase has shown excellent therapeutic potential for treating chronic colitis, enhancing the effects of the probiotic EcN by regulating gut flora and maintaining intestinal barrier function, reducing inflammation symptoms and pathological abnormalities.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Qin Wang, Xin-Meng Jiang, Yan-Ping Gao, Li-Ping Yin, Xiao-Yao Wei, Kai Guo, Xiao-Wei Gao, Li Wang, Chun Zhang
Summary: In this study, various alkyl-aryl and heteroaromatic sulfides were catalyzed by P450 119 peroxygenase in the presence of TBHP. Mutations at position 153 of P450 119 were found to significantly improve the yield and enantioselectivity of sulfoxidation reactions, reaching up to 93% yield and 57% enantiomeric excess. The results suggest the potential of using thermally stable P450 119 peroxygenase for the biosynthesis of chiral sulfoxides, particularly for asymmetric oxidation of heteroaromatic sulfides.
Article
Biotechnology & Applied Microbiology
Jing Zhang, Manyu Liang, Lijuan Wu, Yanhong Yang, Yingjie Sun, Qin Wang, Xiaowei Gao
Summary: This study developed a simple and cost-effective method to digest feathers using Bacillus licheniformis WHU, yielding a hydrolysate that can be directly used as a bioactive nutrient resource. In addition, supplementation with feather hydrolysate had positive effects on intestinal health, immune function, and microbial composition in mice.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Manyu Liang, Yanhong Yang, Jing Zhang, Yingjie Sun, Haixia Peng, Huawei Yi, Qin Wang, Xiaowei Gao
Summary: Vaccines and cell therapeutics based on genetic code expansion are emerging. This study investigated the effects of three non-canonical amino acids (ncAAs) commonly used in these therapies on mice. The results found that one of the ncAAs had fewer negative effects on physiological and gut microecological balance, suggesting its suitability for genetic code expansion-based treatments.
MICROBIAL BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Gao Xiao-Wei, Wei Si-Ping, Wang Qin
Summary: Genetic codon expansion enables manipulation of protein structure and function, and has wide applications in the development of protein and peptide drugs.
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Chemistry, Physical
Yao Zhu, Yue Qi, Xinghua Guo, Meicheng Zhang, Zhimin Jia, Chuanqin Xia, Ning Liu, Chiyao Bai, Lijian Ma, Qin Wang
Summary: This study introduces a novel COF & SOF bicrystalline composite with improved stability, adsorption performance and recycling rate compared to single COF and SOF materials. The bicrystalline composite combines the advantages of COFs and SOFs, providing a potential solution to the limitations faced by traditional SOFs and enhancing the functionality of COFs for practical applications.
JOURNAL OF MATERIALS CHEMISTRY A
(2021)
