Effect of cultivar, cultivation year and dehulling on raffinose family oligosaccharides in Australian sweet lupin (Lupinus angustifolius L.)

The aim of this study was to determine the effect of cultivar, cultivation year and dehulling on raffinose family oligosaccharides (RFOs) in current cultivars of Australian sweet lupin. Seed samples of ten cultivars grown in 2011, 2012 and 2013 were used in the study. Both whole seed and dehulled lupin samples were analysed for RFOs by high-performance liquid chromatography with evaporative light scattering detector. Lupin cultivar had a significant effect on RFO contents. Total RFO contents in whole seed and dehulled lupin samples varied between 7.3-10.1 g/100 g DM and 7.6-16.8 g/100 g DM, respectively. Belara and Mandelup cultivars had high levels of RFOs indicating the suitability for functional foods with prebiotic effect. Gungurru and PBA Barlock contained low levels of RFOs and recommended for lupin-enriched foods with low flatulence effect. Cultivation year with similar climatic conditions had no significant effect on RFO contents. Dehulling increases raffinose, stachyose and total RFO contents in lupin.