Article
Pharmacology & Pharmacy
Stine Egebro Birk, Mette Dalskov Mosgaard, Rolf Bech Kjeldsen, Anja Boisen, Rikke Louise Meyer, Line Hagner Nielsen
Summary: Beneficial microbes in the oral cavity form biofilms that protect pathogenic bacteria, emphasizing the importance of oral hygiene. Research shows that microcontainers can effectively release the antimicrobial peptide nisin, with a faster antibacterial effect compared to solution-based delivery, showing potential for treating oral diseases.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Agriculture, Multidisciplinary
Shaoqi Qu, Yiming Han, Ying Liu, Jiajia Zhu, Ulas Acaroz, Jianzhong Shen, Kui Zhu
Summary: This study functionalizes milk exosomes with phosphatidylserine to improve the solubility of BCS class II and IV drugs, facilitating oral drug delivery. Loading a natural flavonoid, α-mangostin, into exosomes enhances the antibacterial efficiency and demonstrates significant therapeutic efficacy in two animal infection models. This work expands the application of milk exosomes in improving drug solubility and enhancing the efficacy of oral administration.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
S. Panda, S. Hajra, A. Kaushik, H. G. Rubahn, Y. K. Mishra, H. J. Kim
Summary: This comprehensive review explores the potential of smart nanomaterials in cancer management, focusing on various types of nanomaterials and their applications in diagnostics, targeted drug delivery, and therapy. It critically analyzes recent studies and discusses challenges and prospects in the field, proposing a novel multidisciplinary and combinational approach for personalized cancer management.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Engineering, Biomedical
Tien-Jen Chang, Rolf Bech Kjeldsen, Juliane Fjelrad Christfort, Eduard Marzo Vila, Tommy Sonne Alstrom, Kinga Zor, En-Te Hwu, Line Hagner Nielsen, Anja Boisen
Summary: This study investigates the use of microscale stereolithography 3D printing to fabricate radiopaque microcontainers with enhanced mucoadhesive geometries, aiming to improve bioavailability. The results show that microcontainers with adhering features exhibit increased mucoadhesion. In vivo studies using imaging techniques reveal the time-dependent gastrointestinal location and spatial dynamics of microcontainers.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Federica Sommonte, Ilaria Arduino, Rosa Maria Iacobazzi, Mattia Tiboni, Federico Catalano, Roberto Marotta, Martina Di Francesco, Luca Casettari, Paolo Decuzzi, Angela Assunta Lopedota, Nunzio Denora
Summary: After 20 years of research, nanoscale delivery systems for biologicals in nanomedicine have become clinically relevant. Microfluidic-based production of solid lipid nanoparticles (SLNs) is shown to be efficient in encapsulating and delivering lysozyme (LZ), with smaller size and higher encapsulation efficacy compared to conventional methods. This study demonstrates the potential of microfluidic production of SLNs for encapsulation and delivery of complex biological molecules.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Review
Biotechnology & Applied Microbiology
Kai Chen, Yonghui Li, Youbin Li, Yinfeng Tan, Yingshuo Liu, Weisan Pan, Guoxin Tan
Summary: Stimuli-responsive nanofibers prepared by electrospinning are ideal materials due to their large surface area and quick response to external stimuli. As intelligent drug delivery platforms, these nanofibers can efficiently load drugs and release them on-demand under specific conditions such as light, temperature, magnetic field, ultrasound, pH or ROS, showing great potential in various biomedical applications. Therefore, this paper reviews the recent trends of stimuli-responsive electrospun nanofibers as intelligent drug delivery platforms in the field of biomedicine.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Materials Science, Biomaterials
Qikun Cheng, Mingzhi Xie, Guowei Li, Wei Xue, Lexiang Zeng, Dong Ma
Summary: This study reported a new strategy to decrease alcohol absorption by using bacteria in the upper gastrointestinal tract, which could relieve acute alcohol intoxication. A bacteria-loaded gastro-retention oral delivery system was constructed, which maintained a suspension ratio of over 30% in simulated gastric fluid for 4 minutes and decreased the alcohol concentration in vitro. In vivo imaging results showed that the system remained in the upper GI tract for 24 hours and reduced alcohol absorption by 41.9%. Mice administered with the system showed normal gait, smooth coat, and less liver damage. The study concluded that the bacteria-loaded gastro-retention oral delivery system has the potential in the treatment of alcohol abuse.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Qikun Cheng, Mingzhi Xie, Guowei Li, Wei Xue, Lexiang Zeng, Dong Ma
Summary: This study reported a new strategy of directly decreasing alcohol absorption by utilizing bacteria in the upper gastrointestinal (GI) tract. The researchers constructed a bacteria-loaded gastro-retention oral delivery system with a pore structure, which successfully relieved acute alcohol intoxication in mice. The system displayed good protection for the bacteria, decreased alcohol concentration, and reduced alcohol absorption in vitro and in vivo experiments, suggesting its potential in the treatment of alcohol abuse.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Chemical
Yunpeng Wang, Yu Zheng, Kai Zhao, Suli Wu, Benzhi Ju, Shufen Zhang, Wenbin Niu
Summary: The study introduces a strategy for fabricating magnetic photonic crystal microspheres, which can be applied in biomedical, micro/nano motors, and sensors. By combining a strongly magnetic microinner core with an inorganic PC shell, the assembly and motion of MPCMs are achieved, leading to the construction of a multifunctional microsensor with quadruple sensing capabilities.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Chong Zhang, Xiaorong Gao, Xinxiu Ren, Ting Xu, Qiang Peng, Yixin Zhang, Zhenhua Chao, Wenning Jiang, Lingyun Jia, Lulu Han
Summary: This study presents a bacteria-induced encapsulation strategy to enhance the oral bioavailability of probiotics. The encapsulation is achieved through assembling colloids to form shells that protect probiotics in the harsh gastrointestinal tract and improve their oral bioavailability. The encapsulated probiotics demonstrate durable intestinal adhesion, continuous proliferation activity, good oral biosafety, and excellent therapeutic efficacy in a colitis mouse model.
Article
Chemistry, Multidisciplinary
Chong Zhang, Xiaorong Gao, Xinxiu Ren, Ting Xu, Qiang Peng, Yixin Zhang, Zhenhua Chao, Wenning Jiang, Lingyun Jia, Lulu Han
Summary: A bacteria-induced encapsulation strategy is used to enhance the oral bioavailability of probiotics. Using host-guest interaction-driven attraction and electrostatic repulsion, amino-modified poly-beta-cyclodextrin and tannic acid are assembled into colloids (NTc) that can encapsulate bacteria within 10 seconds. The compact, thick, and positively charged NTc shells provide strong resistance against simulated gastric fluid, with a survival rate 7500 times higher than commercial enteric material L100.
Review
Cell Biology
Ali Jangjou, Amir Hossein Meisami, Kazem Jamali, Mohammad Hadi Niakan, Milad Abbasi, Mostafa Shafiee, Majid Salehi, Ahmad Hosseinzadeh, Ali Mohammad Amani, Ahmad Vaez
Summary: Microbubbles are small bubbles used as drug carriers that can release drugs through ultrasound. They have great therapeutic potential in treating common diseases.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Review
Chemistry, Multidisciplinary
Wei Sang, Ruiping Zhang, Xiangyang Shi, Yunlu Dai
Summary: Nanofibers are wire-like materials with nanoscale diameters and specific length diameter ratios. Metallized nanofibers enhance the performance of conventional nanofibers through electrostatic spinning doping, chemical modification, and loading approaches. Due to their unique physical and chemical properties, metallized nanofibers have diverse applications in physical chemistry, materials science, and battery preparation, and are gradually expanding into the biomedical field.
Article
Chemistry, Multidisciplinary
Philip H. R. Carlsen, Rolf Bech Kjeldsen, Gabriel K. Pedersen, Dennis Christensen, Line Hagner Nielsen, Anja Boisen
Summary: Oral vaccination has gained attention recently due to patient compliance and logistics. However, developing oral subunit vaccines is challenging. Micro technology, such as Microcontainers (MCs), can be used to overcome these challenges by facilitating protection and effective delivery of vaccine components in the gastrointestinal tract. The study tests MCs for oral delivery of the C. trachomatis vaccine candidate CTH522, and finds that alpha-GalCer is the most efficient adjuvant for the oral vaccine. The study also suggests that larger animals should be used in future trials with MCs due to potential clearance issues in mice.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Navid Rabiee, Mohammad Rabiee, Soheil Sojdeh, Yousef Fatahi, Rassoul Dinarvand, Moein Safarkhani, Sepideh Ahmadi, Hossein Daneshgar, Fatemeh Radmanesh, Saeid Maghsoudi, Mojtaba Bagherzadeh, Rajender S. Varma, Ebrahim Mostafavi
Summary: In this study, an unpredictable method using high-gravity was introduced to synthesize NH2-MIL-53 for the first time to enhance sensitivity to genetic materials and cellular protein structures. By optimizing the concept phenomenon, interactions between biomolecules and substrates were improved, achieving detection limits of 10 pM for Anti-cas9 protein, 20 pM for ssDNA, less than 10 pM for sgRNA, and around 10 nM for recombinant SARS-CoV-2 spike antigen.
Article
Chemistry, Multidisciplinary
Zhenping Cao, Rui Liu, Chuhan Wang, Sisi Lin, Lu Wang, Yan Pang
Summary: A method for visualizing gut microbiota derived outer membrane vesicles (OMVs) has been developed by preparing nanoprobes with fluorescence-activating and absorption-shifting tag. These nanoprobes can track gut microbiota associated OMVs anaerobically and demonstrate flexibility in fluorescence turn-on/off and reversible switchable emission bands for intelligent and dual-color imaging. The behaviors of microbiota OMVs, including inhibiting pathogenic strains and repairing the intestinal barrier, have been tracked and identified in the gut. These findings suggest the potential of OMVs in remitting inflammation in colitis. This work provides an approach to visualize the dynamics of the gut microbiota and uncover potential targets for disease intervention.
Article
Chemistry, Multidisciplinary
Hejin Jiang, Rui Liu, Lu Wang, Xinyue Wang, Mengmeng Zhang, Sisi Lin, Zhenping Cao, Feng Wu, Yingbin Liu, Jinyao Liu
Summary: Chirality plays a critical role in biological systems, and this study demonstrates the potential of using supramolecular chiral polymer micelles (SCPMs) to activate the immune system for disease prevention and treatment.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ying Liu, Mengmeng Zhang, Xinyue Wang, Fengmin Yang, Zhenping Cao, Lu Wang, Jinyao Liu
Summary: This article reports a method of treating cancer and protecting susceptible patients from lethal infections (such as COVID-19) by dressing bacteria with a hybrid immunoactive nanosurface. The dressed bacteria release immunoactivators to stimulate immune cells, promoting immune responses against tumors and viruses.
ADVANCED MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Huilong Luo, Feng Wu, Xinyue Wang, Sisi Lin, Mengmeng Zhang, Zhenping Cao, Jinyao Liu
Summary: Wrapping bacterial cells with an adhesive drug-loadable nanocoating allows for the encoding of bacterial colonization and therapeutic modality. Encoded bacteria can colonize a wide range of biointerfaces and carry various cargoes for multimodal therapy. In vivo studies demonstrate that orally ingested encoded bacteria exhibit significantly improved intestinal colonization and synergistically enhanced treatment efficacy. This work reveals a versatile platform for advanced microbial therapy through the encoding of bacterial colonization and modality with a single-cell coating.
Editorial Material
Engineering, Biomedical
Lu Wang, Jinyao Liu
Summary: The targeted delivery of butyrate to the lower gastrointestinal tract using polymeric nanoscale micelles restores intestinal homeostasis in mouse models of peanut allergy and colitis.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Haiyan Guo, Zhenping Cao, Juanjuan Li, Zhenzhen Fu, Sisi Lin, Lu Wang, Jinyao Liu
Summary: Photosensitizer-based therapy faces limitations such as weak and easily attenuated photosensitive effects, poor tumor penetration and retention, and the need for multiple irradiation. In this study, bacteria were engineered to express melanin and coated with synthetic photosensitizers, forming a ternary combination for photoacoustic imaging-guided photothermal therapy. The integrated bacteria exhibited stable triple photoacoustic and photothermal effects under monochromatic irradiation, and were able to preferentially colonize hypoxic tumor tissue and generate uniform imaging signals and sufficient heating upon laser irradiation. The results showed inhibited tumor growth and extended survival in different tumor-bearing mouse models, suggesting the potential of bacteria-based innovative photosensitizers for imaging-guided therapy.
Article
Geography, Physical
Zhen-Ping Cao, Ye Yang, Sheng Xu, Hai Xu, Zhaoyan Gu, Guoqiang Chu
Summary: Reconstructing climate variability beyond human records using effective climate proxies is important for understanding past and predicting future climate change. This study utilized the ratio of Be-10(met) to silicate-weathered Be-9 in sediments from a lake in NE China to reconstruct the evolution of the East Asian summer monsoon (EASM) over the past two millennia. The findings suggest two humid periods during specific transitions, which are consistent with previous observations of EASM oscillations in Northeast Asia. The spatial pattern of precipitation shows an anti-phase synchronization between EASM and the Indian summer monsoon, indicating a dry northeastern and wet southwestern mode during the specified periods.
QUATERNARY SCIENCE REVIEWS
(2023)
Article
Multidisciplinary Sciences
Zhongmin Geng, Xinyue Wang, Feng Wu, Zhenping Cao, Jinyao Liu
Summary: This study presents a cytocompatible approach of biointerface mineralization to generate an ultraresistant and self-removable coating on bacterial surface, addressing the challenges faced by bacteria during the manufacturing process. The mineral coating provides bacteria with resistance against oxygen exposure, ultraviolet irradiation, and ethanol. Upon oral ingestion, the coating actively neutralizes gastric acid and releases encapsulated bacteria through a rapid double-decomposition reaction. Furthermore, the generated calcium ions trigger bile acid micellar aggregation, enabling dual exemptions from gastric acid and bile acid insults for uncompromised bacterial viability. Supported by therapeutic efficacy in colitis mice, biointerface mineralization offers a versatile platform for developing next-generation living oral biotherapeutics.
Article
Multidisciplinary Sciences
Xinyue Wang, Sisi Lin, Lu Wang, Zhenping Cao, Mengmeng Zhang, Yifan Zhang, Rui Liu, Jinyao Liu
Summary: Outer membrane vesicles (OMVs) released by Akkermansia muciniphila can restore gut microbiota balance, stimulate mucosal immunoglobulin A response, and maintain intestinal barrier integrity by entering intestinal epithelial cells. Transplanting gut microbiota-derived OMVs can alleviate colitis and enhance anti-PD-1 therapy against colorectal cancer. This study highlights the important role of gut microbiota-derived OMVs in intestinal ecology and provides a new target for disease intervention and treatment.
Article
Chemistry, Multidisciplinary
Xiao Kuang, Ying Liu, Huilong Luo, Qian Li, Feng Wu, Chunhai Fan, Jinyao Liu
Summary: A triggerable prodrug nanocoating is reported for combination treatment, addressing the limitations of current combination strategies. The nanocoating enables the on-demand activation of living microbial and small-molecular therapeutics, leading to enhanced treatment efficacy.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Multidisciplinary
Hejin Jiang, Zhenping Cao, Ying Liu, Rui Liu, Yan Zhou, Jinyao Liu
Summary: This review discusses the strategies for preparing bacteria-based living probes, including biological engineering, chemical modification, intracellular loading, and optical manipulation. It summarizes the recent progress of these probes in different imaging modalities and highlights their applications in bioimaging and diagnosis of bacterial infections, cancers, and intestine-associated diseases. The advantages, challenges, and future perspectives of bacteria-based living probes are also discussed.
Article
Chemistry, Multidisciplinary
Fangjie Chen, Mengmeng Zhang, Fengmin Yang, Lu Wang, Jinyao Liu, Junqiu Liu, Yan Pang
Summary: This study presents the development of dual-antigen-displaying nanovaccines to treat cancer and prevent infection. The nanovaccines are shown to reverse immune suppression and delay tumor growth while inducing protective immune responses.
Review
Chemistry, Multidisciplinary
Sisi Lin, Feng Wu, Yifan Zhang, Huan Chen, Haiyan Guo, Yanmei Chen, Jinyao Liu
Summary: The past decade has seen significant progress in bacteria-based living agents, with their unique characteristics enabling genetic manipulation, rapid proliferation, colonization, and disease site targeting. However, translating these agents to clinical applications remains challenging due to environmental susceptibility, toxic side effects, and limited accumulation at target sites. Surface modification of bacteria offers a solution by chemically altering components and introducing new functions. This review examines the synthesis, functionalization, and biomedical applications of surface-modified bacteria, highlighting the advantages, limitations, and future challenges in utilizing these agents for bioimaging, diagnosis, and therapy.
CHEMICAL SOCIETY REVIEWS
(2023)
