Article
Biotechnology & Applied Microbiology
Claudia Cicione, Gianluca Vadala, Giuseppina Di Giacomo, Veronica Tilotta, Luca Ambrosio, Fabrizio Russo, Biagio Zampogna, Francesca Cannata, Rocco Papalia, Vincenzo Denaro
Summary: This study aimed to compare the biological properties of micro-fragmented adipose tissue (MAT) and nanofat adipose tissue (NAT) obtained with different techniques. The results showed that NAT had the highest content of extracellular matrix, cell replication, and stemness gene expression, providing valuable clues for the clinical use of adipose tissue derivatives.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Sara Al-Ghadban, Maria Artiles, Bruce A. Bunnell
Summary: This article discusses the progress and applications of stem cell-based regenerative medicine in the past decade. Adult stem cells from adipose tissue show promise in treating various diseases and regenerating damaged tissues. Recent developments focus on creating more complex structures, such as organoids and tissue elements.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Instruments & Instrumentation
Hamid Reza Aghayan, Motahareh Sheikh Hosseini, Mahdi Gholami, Fereshteh Mohamadi-jahani, Akram Tayanloo-Beik, Sepideh Alavi-Moghadam, Moloud Payab, Parisa Goodarzi, Mohammad Abdollahi, Bagher Larijani, Babak Arjmand
Summary: This study investigated the regenerative effects of tissue-engineered dressings composed of human amniotic membrane graft (AM) and two types of mesenchymal stem cells (MSCs) in a rat excisional wound model. The results demonstrated that MSC-seeded AMs had superior effects on wound closure rate, re-epithelialization, angiogenesis, and collagen remodeling compared to the control groups, with placenta-derived MSCs (PLMSCs) showing better healing effects. Seeding of MSCs on AM boosted its regenerative effects in wound treatment, with PLMSCs having superior effects to ADMSCs in the excisional wound rat model.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Zhaoqi Yuan, Zhu Zhu, Fangxing Zhu, Feixue Ding, Yinmin Wang, Xiuxia Wang, Xusong Luo, Jun Yang, Fei Liu, Di Sun
Summary: The study found that ADSCs can enhance proliferation, migration, invasion, and angiogenesis of DFSP cells, with increased expression of beta-type platelet-derived growth factor receptor, collagen type I alpha 1 chain, vascular endothelial growth factor, hepatocyte growth factor, and basic fibroblast growth factor.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Victor J. Costela-Ruiz, Lucia Melguizo-Rodriguez, Chiara Bellotti, Rebeca Illescas-Montes, Deborah Stanco, Carla Renata Arciola, Enrico Lucarelli
Summary: The success of regenerative medicine depends on the appropriate selection of mesenchymal stem cell (MSC) sources, as they influence the regenerative milieu. MSCs from bone marrow, adipose tissue, and dental tissue are all potential candidates for oral and dental regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Zuxiang Chen, Yanzhi Ge, Li Zhou, Ting Li, Bo Yan, Junjie Chen, Jiefeng Huang, Wenxi Du, Shuaijie Lv, Peijian Tong, Letian Shan
Summary: Nanofat demonstrates significant efficacy in alleviating joint pain, protecting cartilage, promoting chondrocyte viability and migration, with molecular regulatory effects similar to adipose tissue-derived stem cells. Clinical data shows Nanofat relieves pain and repairs damaged cartilage in OA patients without adverse events.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Jan Rozanc, Lidija Gradisnik, Tomaz Velnar, Minja Gregoric, Marko Milojevic, Bostjan Vihar, Boris Gole, Uros Maver
Summary: PV-ADMSCs are a type of mesenchymal stem cells isolated from paravertebral adipose tissue, showing high proliferation rate and strong osteogenic potential, making them a promising source for therapeutic applications.
Article
Biotechnology & Applied Microbiology
Qiulin He, Junxin Lin, Fanghao Zhou, Dandan Cai, Yiyang Yan, Yejie Shan, Shufang Zhang, Tiefeng Li, Xudong Yao, Hongwei Ouyang
Summary: Nonuniform microstretching (NUMS), a natural phenomenon in bone tissues, promotes osteogenic differentiation and has potential positive effects on bone healing.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Katarzyna Czerwiec, Malgorzata Zawrzykraj, Milena Deptula, Aneta Skoniecka, Agata Tyminska, Jacek Zielinski, Adam Kosinski, Michal Pikula
Summary: Adipose-derived mesenchymal stromal cells (AD-MSCs) have gained significant attention due to their easy availability from fat tissue and their regenerative and immunomodulatory properties. These cells show great potential in stem cell-based therapies for wound healing, orthopedic, cardiovascular, and autoimmune diseases. Numerous clinical trials have provided evidence for the effectiveness of AD-MSCs. This article presents current knowledge on AD-MSCs, their applications in pre-clinical models and clinical studies, and highlights the potential for chemically or genetically modified AD-MSCs as the next generation of stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Jana C. Blum, Thilo L. Schenck, Alexandra Birt, Riccardo E. Giunta, Paul S. Wiggenhauser
Summary: In this study, the combination of cell-derived artificial extracellular matrix with 3D printed polycaprolactone scaffolds showed promising results in improving bioactivity and differentiation of human adipose derived stem cells. The ac/aoPCL scaffolds demonstrated good biocompatibility and enhanced chondrogenic and osteogenic differentiation potential.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Martina Semenzato, Ludovica Zambello, Stefania Fumarola, Enrico Motta, Luana Piroli, Luca Scorrano, Camilla Bean
Summary: This study presents a simple mechanical dissociation procedure for collecting mesenchymal stem cells and soluble fractions from lipoaspirates. A self-contained cell purification device called IStemRewind was used for one-step purification of cells and soluble material, offering a potential solution for rapid and efficient isolation of MSCs and immunomodulatory soluble factors.
Review
Engineering, Biomedical
Arne Peirsman, Huu Tuan Nguyen, Michiel Van Waeyenberge, Carlos Ceballos, Johana Bolivar, Satoru Kawakita, Florian Vanlauwe, Zuzana Tirpakova, Sofie Van Dorpe, Lana Van Damme, Marvin Mecwan, Menekse Ermis, Surjendu Maity, Kalpana Mandal, Rondinelli Herculano, Bernard Depypere, Lore Budiharto, Sandra Van Vlierberghe, Olivier De Wever, Phillip Blondeel, Vadim Jucaud, Mehmet Remzi Dokmeci, Ali Khademhosseini
Summary: Soft tissue defects are a common challenge in clinical practice, usually caused by trauma, congenital anomalies, and oncological surgery. Current reconstruction options have important disadvantages, but vascularized adipose tissue engineering (VATE) strategies could provide solutions. This review summarizes the characteristics of functional adipose tissue, discusses different VATE techniques, and points out current challenges and future perspectives.
Review
Veterinary Sciences
Hussein M. M. El-Husseiny, Eman A. A. Mady, Mahmoud A. Y. Helal, Ryou Tanaka
Summary: The use of stem cells in veterinary practice is rapidly evolving, with significant research efforts focused on both experimental animals and clinical cases. Stem cells, particularly mesenchymal stem cells (MSCs), are promising for therapeutic applications due to their immunomodulatory capabilities and ease of isolation. However, challenges remain in their clinical implementation for treating various animal diseases, driving further advancements in stem cell therapies.
VETERINARY SCIENCES
(2022)
Review
Dermatology
Zahra Hajimortezayi, Narges Daei, Nasim Gholizadeh, Mana Zakeri, Farah Alhili, Sajedeh Hasanzadeh, Arezoo Hormozi, Faezeh Ebrahimi, Majid Zamani
Summary: Fat transplantation is a safe and effective method for facial rejuvenation and regenerative medicine due to its biocompatibility and lack of immune reaction. Adipose tissue contains high percentages of mature stem cells, which contribute to tissue regeneration. The size of the transplanted fat affects the results in different surgical sites.
JOURNAL OF COSMETIC DERMATOLOGY
(2023)
Review
Orthopedics
Karthikeyan P. Iyengar, Vijay Kumar Jain, Arulkumar Nallakumarasamy, Madhan Jeyaraman
Summary: Virtual Fracture Clinics (VFCs) are a new service that aims to provide an alternative to traditional fracture and minor injuries care. This pilot study explores the possibility of using telemedicine technology and a virtual fracture clinical assessment pathway to remotely assess patients in India. However, there are several challenges in implementing this model in India, including regulations, internet connectivity, and concerns about medico-legal implications. The limitations and shortcomings of VFCs in India can be addressed with effective communication, enhanced documentation, appropriate training, and information governance.
INDIAN JOURNAL OF ORTHOPAEDICS
(2023)
Article
Orthopedics
Sathish Muthu
Summary: With the development of machine learning and artificial intelligence, a machine learning-assisted platform has been developed to aid in the time-consuming process of article screening in systematic reviews in orthopaedic surgery. The platform significantly improves the rate of identifying relevant records compared to the traditional screening method, but its efficiency decreases as the complexity of the research question increases.
INTERNATIONAL ORTHOPAEDICS
(2023)
Article
Orthopedics
Satvik N. Pai, Madhan Jeyaraman, Nicola Maffuli, Naveen Jeyaraman, Filippo Migliorini, Ashim Gupta
Summary: To address the lack of uniformity and standard procedure in obtaining informed consent for total knee arthroplasty (TKA), researchers developed a pre-designed, evidence-based informed consent form. After one year of use, it was found to be legally sound and beneficial for both orthopaedic surgeons and patients.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Sathish Muthu, Madhan Jeyaraman, Aditya Narula, V. R. Ravi, Avinash Gandi, Manish Khanna, Nicola Maffulli, Ashim Gupta
Summary: This study aimed to determine the influence of subjective factors (age, sex, and comorbidities) and procedure-specific factors (aspiration volume) on the yield of progenitor cells in bone marrow aspiration concentrate (BMAC) harvested from the iliac crest. A retrospective analysis on 58 patients undergoing BMAC therapy revealed that age, comorbidities, and aspirate volume significantly affected progenitor cell yield, while sex did not have a significant impact. These findings highlight the importance of considering individual and procedural factors when assessing the effectiveness of BMAC therapy.
Review
Biochemistry & Molecular Biology
Fabio Ramos Costa, Mariana Ramos Costa Marques, Vinicius Calumby Costa, Gabriel Silva Santos, Rubens Andrade Martins, Marcia da Silva Santos, Maria Helena Andrade Santana, Arulkumar Nallakumarasamy, Madhan Jeyaraman, Joao Vitor Bizinotto Lana, Jose Fabio Santos Duarte Lana
Summary: Musculoskeletal diseases, such as osteoarthritis and tendinopathies, have a significant impact on society and individuals' quality of life. Intra-articular hyaluronic acid (HA) has emerged as a safe and effective treatment option for these conditions, providing multiple benefits such as lubrication, anti-inflammation, and stimulation of cellular activity. This review addresses the scientific, commercial, and clinical aspects of HA, providing physicians with a deeper understanding of disease processes, tissue repair mechanisms, and the advantages of different HA types.
Article
Public, Environmental & Occupational Health
Naveen Jeyaraman, Madhan Jeyaraman, Arulkumar Nallakumarasamy, K. Shanmugapriya, Suraj Adhikari, Rishikesh Rijal, Ankush Asija, Yub Raj Sedhai, Sanjit Sah, Aroop Mohanty, D. Katterine Bonilla-Aldana, Ranjit Sah
Summary: The diverse manifestations of osteoarticular brucellosis often lead patients to seek help from general practitioners, orthopaedic and rheumatology specialists. The lack of disease-specific symptoms is a major cause of delayed diagnosis. With the increasing cases of spinal brucellosis, there is a need for literature on its systematic management. We conducted a study and formulated a classification system based on clinical, serological, and radiological analysis of 25 confirmed cases.
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Medicine, General & Internal
Madhan Jeyaraman, Swaminathan Ramasubramanian, Naveen Jeyaraman, Arulkumar Nallakumarasamy, Shilpa Sharma
Summary: Despite a decrease in publication, case reports remain significant in medical literature for providing detailed descriptions of individual patient cases and valuable insights for future management. These reports follow a standardized structure and contribute to evidence-based medicine by detecting new therapies and adverse events. They are essential for documenting rare occurrences, assisting clinicians, and communicating novel information to busy medical professionals.
WORLD JOURNAL OF CLINICAL CASES
(2023)
Article
Clinical Neurology
Juan P. Cabrera, Matthew F. Gary, Sathish Muthu, S. Tim Yoon, Ho-Joong Kim, Samuel K. Cho, Stipe Corluka, Stephen J. Lewis, So Kato, Zorica Buser, Jeffrey C. Wang, Patrick C. Hsieh, AO Spine Knowledge Forum Degenerative
Summary: This study conducted a survey among spine surgeons worldwide to investigate the preferences and practices regarding wound drain utilization in lumbar degenerative surgery. The majority of surgeons preferred subfascial drains and made decisions on drain removal based on either duration or outputs. Surgeon's age, region, and experience were found to be associated with drain use and removal criteria.
GLOBAL SPINE JOURNAL
(2023)
Review
Multidisciplinary Sciences
Madhan Jeyaraman, Ramya Lakshmi Rajendran, Sathish Muthu, Naveen Jeyaraman, Shilpa Sharma, Saurabh Kumar Jha, Purushothaman Muthukanagaraj, Chae Moon Hong, Lucas Furtado da Fonseca, Jose Fabio Santos Duarte Lana, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: Globally, neurological diseases, particularly Alzheimer's disease, have a significant impact on healthcare professionals. Alzheimer's disease is a progressive neurological disorder that leads to the loss of neurons and synapses in the brain. The treatment of Alzheimer's disease remains controversial, and this paper examines the potential use of genetically modified stem cells and extracellular vesicles in regenerative medicine for Alzheimer's disease.
Article
Medicine, General & Internal
Sankalp Yadav, Gautam Rawal, Madhan Jeyaraman
Summary: We present a rare case of tuberculosis involving the first metatarsal of the left foot with congenital hallux valgus, without pulmonary involvement. The diagnosis was confirmed using radiology, cartridge-based nucleic acid amplification test, and pus culture. Unfortunately, the patient was lost to follow-up and could not receive anti-tubercular treatment.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Medicine, General & Internal
Sankalp Yadav, Gautam Rawal, Madhan Jeyaraman
Summary: This case report describes a rare case of primary talonavicular joint tuberculosis in a child without pulmonary involvement. The patient presented with pain and swelling in the right foot, and a detailed work-up confirmed the diagnosis. Conservative management with antitubercular chemotherapy resulted in symptom improvement.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Medicine, General & Internal
Sankalp Yadav, Gautam Rawal, Madhan Jeyaraman
Summary: Tuberculosis of the bones and joints, especially in small bones of the foot, is rare and often associated with delayed diagnosis, ultimately affecting treatment outcomes.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Review
Medicine, General & Internal
Madhan Jeyaraman, Swaminathan Ramasubramanian, Shanmugapriya Kumar, Naveen Jeyaraman, Preethi Selvaraj, Arulkumar Nallakumarasamy, Suresh K. Bondili, Sankalp Yadav
Summary: Social media plays a crucial role in healthcare, medical education, and research, enabling collaboration and information dissemination. While it offers opportunities for patient communication, professional development, and knowledge-sharing, concerns about misinformation, patient privacy breaches, and unprofessional conduct persist.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
