Editorial Material
Cell Biology
Wayne D. Hawkins, Daniel J. Klionsky
Summary: Autophagy proteins often have multiple roles, not only in the process of autophagy but also potentially in other cellular processes.
Review
Physiology
Shengyuan Wang, Hongyan Li, Minghao Yuan, Haixia Fan, Zhiyou Cai
Summary: Adenosine monophosphate-activated protein kinase (AMPK) plays a significant role in maintaining cellular energy homeostasis and is linked with different stages of autophagy.
FRONTIERS IN PHYSIOLOGY
(2022)
Editorial Material
Cell Biology
Xinyu Gong, Lifeng Pan
Summary: The recruitment of ATG12-ATG5-ATG16L1 complex to phagophore, which is crucial in autophagosome formation, relies on the specific interaction between ATG16L1 and WIPI2. It has been found that ATG16L1 contains two distinct WIPI2-binding sites, WBS1 and the newly identified WBS2, and the binding mechanism between ATG16L1 WBS2 and WIPI2 is conserved across species. The integrity of these two WIPI2-binding sites in ATG16L1 is essential for normal autophagic flux.
Article
Cell Biology
Hao-Chun Chang, Ran N. Tao, Chong Teik Tan, Ya Jun Wu, Boon Huat Bay, Victor C. Yu
Summary: Cells deficient in MOAP1 are hypersensitive to cell death mediated by starvation, and exhibit impaired macroautophagy/autophagy signaling induced by EBSS treatment. MOAP1 interacts with LC3 to promote efficient phagophore closure during starvation.
Editorial Material
Cell Biology
Tatsuro Maruyama, Nobuo N. Noda
Summary: Atg8, a central factor in autophagosome biogenesis, exhibits physicochemical activity towards membranes and cargo recognition, essential for efficient autophagosome formation, but its mechanistic roles remain open for further exploration.
Article
Biochemistry & Molecular Biology
Alexandra Polyansky, Oren Shatz, Milana Fraiberg, Eyal Shimoni, Tali Dadosh, Muriel Mari, Fulvio M. Reggiori, Chao Qin, Xianlin Han, Zvulun Elazar
Summary: This study investigates the role of phosphatidylcholine (PC) in autophagy using yeast as a model organism. The results show that PC is important for autophagosome formation and that disruption of PC biosynthesis leads to changes in lipid composition and impaired autophagic flux.
Editorial Material
Cell Biology
Vikramjit Lahiri, Daniel J. Klionsky
Summary: Nguyen et al. discovered a previously unexplored role for mammalian ATG4s in mitophagy, involving the recruitment of ATG9A-containing vesicles. Their article highlights the use of a novel AI-directed analysis technique for FIB-SEM imaging to demonstrate the role of ATG4s in promoting phagophore growth and establishing phagophore-ER contacts.
Article
Cell Biology
Wei Wan, Wei Liu
Summary: STING interacts directly with WIPI2 to recruit WIPI2 to STING-positive vesicles for LC3 lipidation and autophagosome formation. STING and PtdIns3P competitively bind to the FRRG motif of WIPI2, resulting in mutual inhibition between STING-induced and PtdIns3P-dependent autophagy. Additionally, the STING-WIPI2 interaction is essential for clearing cytoplasmic DNA and attenuating activated cGAS-STING signaling.
Article
Cell Biology
Hong Huang, Qinqin Ouyang, Kunrong Mei, Ting Liu, Qiming Sun, Wei Liu, Rong Liu
Summary: This study reveals that both acetylation and phosphorylation modifications control the function of SCFD1 in autophagosome-lysosome fusion. KAT2B/PCAF catalyzes the acetylation of SCFD1, while SIRT4 deacetylates it. Additionally, AMPK-controlled phosphorylation disrupts the interaction between SCFD1 and KAT2B and inhibits SCFD1 acetylation. Furthermore, SCFD1 acetylation inhibits autophagic flux by blocking SNARE complex formation.
Article
Multidisciplinary Sciences
Anna Bieber, Cristina Capitanio, Philipp S. Erdmann, Fabian Fiedler, Florian Beck, Chia-Wei Lee, Delong Li, Gerhard Hummer, Brenda A. Schulman, Wolfgang Baumeister, Florian Wilfling
Summary: By combining different research methods, we directly revealed the structural progression of autophagosome biogenesis and organelle interactome within yeast cells. These findings have important implications for understanding the contribution of different membrane sources during autophagy and the forces shaping and driving phagophores.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Noriko Toda, Takeya Sato, Mikio Muraoka, Delan Lin, Masaki Saito, Guanje Li, Qui-Chao Song, Teruyuki Yanagisawa, Masanori Yamauchi
Summary: This study evaluated the effects of Doxorubicin on the mitochondrial quality control system and regulation of mitochondrial respiration and autophagy in an in vitro cell culture model. The results showed that Doxorubicin affected the mitochondrial quality control system, leading to a fission-dominant morphology and impaired regulation of mitochondrial respiration, thereby increasing oxidative stress and inhibiting the progression of autophagy.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Materials Science, Multidisciplinary
Yiming Geng, Shengyun Huang, Li Ma, Mingyang Li, Enli Yang, Yiming Li, Dongsheng Zhang, Xiao Fu, Haiwei Wu
Summary: Autophagy is a cellular process that captures and degrades intracellular components to maintain metabolism and homeostasis. In late stage tumorigenesis, autophagy promotes the survival, growth, and aggressiveness of tumors, as well as drug resistance. Nanoparticles can induce autophagy and offer a new perspective for tumor therapy strategies.
APPLIED MATERIALS TODAY
(2023)
Article
Cell Biology
Ayelen Valko, Sebastian Perez-Pandolfo, Eleonora Sorianello, Andreas Brech, Pablo Wappner, Mariana Melani
Summary: Hypoxia induces autophagy in Drosophila melanogaster, which is essential for adaptation and survival. The process involves a bona fide autophagic response with waves of autophagosome formation and maturation, and is induced cell autonomously in different tissues. Autophagy under hypoxic conditions can be studied using D. melanogaster as a model organism, offering insights into its role in hypoxia-associated pathologies and developmentally regulated processes.
Article
Immunology
Ainhoa Plaza-Zabala, Virginia Sierra-Torre, Amanda Sierra
Summary: Autophagy is a critical cellular process that helps cells clear debris and maintain innate immune function. Understanding autophagic flux is important for studying the stages of autophagy. To dissect the regulation and impact of autophagy, systematic analysis of both autophagosome formation and degradation stages is necessary.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Supansa Pantoom, Georgios Konstantinidis, Stephanie Voss, Hongmei Han, Oliver Hofnagel, Zhiyu Li, Yao-Wen Wu
Summary: The study demonstrates that RAB33B plays a crucial role in recruiting the ATG16L1 complex during autophagy, with the two proteins determining each other's localization on phagophores. Additionally, ATG16L1 is identified as a novel RAB-binding protein that can induce active conformation in RAB proteins. These findings reveal a new mechanism for autophagosome formation.