ATP-responsive biomolecular condensates tune bacterial kinase signaling

1,6-Hexanediol, commonly used to dissolve liquid-liquid phase separated condensates, directly impairs kinase and phosphatase activities

(2021) Robert Düster et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Ligand effects on phase separation of multivalent macromolecules

(2021) Kiersten M. Ruff et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Intrinsic disorder in protein domains contributes to both organism complexity and clade-specific functions

(2021) Chao Gao et al.   Scientific Reports

Mechanistic dissection of increased enzymatic rate in a phase-separated compartment

(2021) William Peeples et al.   Nature Chemical Biology

Phase separation provides a mechanism to reduce noise in cells

(2020) A. Klosin et al.   SCIENCE

Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus

(2020) Keren Lasker et al.   Nature Microbiology

Caulobacter crescentus: model system extraordinaire

(2020) Sander K. Govers et al.   CURRENT BIOLOGY

Daily Cycles of Reversible Protein Condensation in Cyanobacteria

(2020) Gopal K. Pattanayak et al.   Cell Reports

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular Basis and Ecological Relevance of Caulobacter Cell Filamentation in Freshwater Habitats

(2019) Kristina Heinrich et al.   mBio

Advances in Understanding Stimulus-Responsive Phase Behavior of Intrinsically Disordered Protein Polymers

(2018) Kiersten M. Ruff et al.   JOURNAL OF MOLECULAR BIOLOGY

Spatial organization and dynamics of RNase E and ribosomes inCaulobacter crescentus

(2018) Camille A. Bayas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification of PAmKate as a Red Photoactivatable Fluorescent Protein for Cryogenic Super-Resolution Imaging

(2018) Peter D. Dahlberg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

α-Proteobacterial RNA Degradosomes Assemble Liquid-Liquid Phase-Separated RNP Bodies

(2018) Nadra Al-Husini et al.   MOLECULAR CELL

ATP-Dependent Dynamic Protein Aggregation Regulates Bacterial Dormancy Depth Critical for Antibiotic Tolerance

(2018) Yingying Pu et al.   MOLECULAR CELL

ATP as a biological hydrotrope

(2017) Avinash Patel et al.   SCIENCE

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

A Localized Complex of Two Protein Oligomers Controls the Orientation of Cell Polarity

(2017) Adam M. Perez et al.   mBio

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Super-resolution Imaging of Live Bacteria Cells Using a Genetically Directed, Highly Photostable Fluoromodule

(2016) Saumya Saurabh et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues

(2016) Thomas H. Mann et al.   Nature Communications

MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis

(2016) Adrien Ducret et al.   Nature Microbiology

Kinetically Tunable Photostability of Fluorogen-Activating Peptide-Fluorogen Complexes

(2015) Saumya Saurabh et al.   CHEMPHYSCHEM

A general method to improve fluorophores for live-cell and single-molecule microscopy

(2015) Jonathan B Grimm et al.   NATURE METHODS

The Airyscan detector from ZEISS: confocal imaging with improved signal-to-noise ratio and super-resolution

(2015) Joseph Huff   NATURE METHODS

Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging

(2014) Hideyuki Yaginuma et al.   Scientific Reports

The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity

(2013) Bradley R. Parry et al.   CELL

Oligomerization and higher-order assembly contribute to sub-cellular localization of a bacterial scaffold

(2013) Grant R. Bowman et al.   MOLECULAR MICROBIOLOGY

Quantitative Multicolor Subdiffraction Imaging of Bacterial Protein Ultrastructures in Three Dimensions

(2013) Andreas Gahlmann et al.   NANO LETTERS

A Quantitative Comparison of Single-Dye Tracking Analysis Tools Using Monte Carlo Simulations

(2013) Laura Weimann et al.   PLoS One

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Nonthermal ATP-dependent fluctuations contribute to the in vivo motion of chromosomal loci

(2012) S. C. Weber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lipoic Acid Metabolism in Microbial Pathogens

(2010) M. D. Spalding et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic medium

(2010) Xavier Michalet   PHYSICAL REVIEW E

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

The dynamic interplay between a cell fate determinant and a lysozyme homolog drives the asymmetric division cycle of Caulobacter crescentus

(2008) S. K. Radhakrishnan et al.   GENES & DEVELOPMENT