Review
Cell Biology
Bruna Andrade Aguiar Koga, Leticia Alves Fernandes, Paula Fratini, Mari Cleide Sogayar, Ana Claudia Oliveira Carreira
Summary: Mesenchymal stem cells (MSCs) secrete vesicles known as extracellular vesicles (EVs), which play a crucial role in tissue homeostasis, repair, and therapeutic applications. MSC-derived EVs have advantages such as low immunogenicity and the ability to pass through barriers, making them potential drug delivery agents for various diseases. Extensive in vitro studies have been conducted to understand the mechanisms and signaling pathways of EVs. Clinical trials are underway to explore the therapeutic potential of EVs in Alzheimer's disease, retina degeneration, and COVID-19. This review provides an overview of different EV isolation methods and highlights their therapeutic applications in tissue repair and regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Fernando J. Aguilar-Ayala, Fernando J. Aguilar-Perez, Geovanny Nic-Can, Rafael Rojas-Herrera, Gabriela Chuc-Gamboa, David Aguilar-Perez, Beatriz A. Rodas-Junco
Summary: Biomaterials and stem cells are crucial in regenerative medicine, particularly in the development of autologous therapies. While progress has been made, challenges remain in understanding the characteristics of biomaterials and the molecular mechanisms regulating stem cell behavior.
APPLIED SCIENCES-BASEL
(2022)
Article
Pharmacology & Pharmacy
Giulia Brunello, Federica Zanotti, Martina Trentini, Ilaria Zanolla, Elham Pishavar, Vittorio Favero, Riccardo Favero, Lorenzo Favero, Eriberto Bressan, Massimo Bonora, Stefano Sivolella, Barbara Zavan
Summary: This study found that EXOs derived from dental pulp stem cells of different age donors have varying effects on MSCs. The younger donor group showed a more significant influence on proliferation and commitment ability.
Review
Biochemistry & Molecular Biology
Katarzyna Czerwiec, Malgorzata Zawrzykraj, Milena Deptula, Aneta Skoniecka, Agata Tyminska, Jacek Zielinski, Adam Kosinski, Michal Pikula
Summary: Adipose-derived mesenchymal stromal cells (AD-MSCs) have gained significant attention due to their easy availability from fat tissue and their regenerative and immunomodulatory properties. These cells show great potential in stem cell-based therapies for wound healing, orthopedic, cardiovascular, and autoimmune diseases. Numerous clinical trials have provided evidence for the effectiveness of AD-MSCs. This article presents current knowledge on AD-MSCs, their applications in pre-clinical models and clinical studies, and highlights the potential for chemically or genetically modified AD-MSCs as the next generation of stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Yuxuan Zhong, Xiang Li, Fanglin Wang, Shoushuai Wang, Xiaohong Wang, Xiaohong Tian, Shuling Bai, Di Miao, Jun Fan
Summary: Recent studies have shown that various exosomes can influence the adipogenic differentiation of stem cells. Exosomes play an important role in promoting tissue regeneration and provide new ideas for adipose tissue engineering research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Anatomy & Morphology
Mohsen Ghiasi, Khosrow Jadidi, Mehrdad Hashemi, Hamed Zare, Ali Salimi, Hossein Aghamollaei
Summary: The cornea is fragile and vulnerable to various damages, leading to challenges in treatments such as corneal transplantation. Cell therapy, specifically using Mesenchymal stem cells, has shown promise in corneal regeneration due to their unique characteristics and potential to differentiate into different cell types. MSCs have demonstrated capabilities in treating keratitis, reducing inflammation, and inhibiting neovascularization, making them novel agents for corneal repair.
Review
Biochemistry & Molecular Biology
Victor J. Costela-Ruiz, Lucia Melguizo-Rodriguez, Chiara Bellotti, Rebeca Illescas-Montes, Deborah Stanco, Carla Renata Arciola, Enrico Lucarelli
Summary: The success of regenerative medicine depends on the appropriate selection of mesenchymal stem cell (MSC) sources, as they influence the regenerative milieu. MSCs from bone marrow, adipose tissue, and dental tissue are all potential candidates for oral and dental regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell & Tissue Engineering
Ria Margiana, Alexander Markov, Angelina O. Zekiy, Mohammed Ubaid Hamza, Khalid A. Al-Dabbagh, Sura Hasan Al-Zubaidi, Noora M. Hameed, Irshad Ahmad, R. Sivaraman, Hamzah H. Kzar, Moaed E. Al-Gazally, Yasser Fakri Mustafa, Homayoon Siahmansouri
Summary: MSCs have great potential for immunomodulation and tissue regeneration, and can be used for the treatment of various diseases. Recent advances in MSCs biology have facilitated their clinical application.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Zhaohui Luo, Shimin Chen, Jing Zhou, Chong Wang, Kai Li, Jia Liu, Yujin Tang, Liqiang Wang
Summary: Regenerative medicine is a discipline that focuses on repairing and regenerating damaged tissues and organs. The emergence of aptamers has injected new vitality into this field and enriched the application elements of tissue engineering technology.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell & Tissue Engineering
Yaping Wang, Tianyun Gao, Bin Wang
Summary: Senescence is a hot topic due to the accumulation of senescent cells and inflammatory factors, leading to various senescence-related diseases. Stem cell-based therapy, particularly using mesenchymal stromal cells (MSCs), is considered a promising strategy to alleviate or cure these diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Biochemistry & Molecular Biology
Agnese Gugliandolo, Luigia Fonticoli, Oriana Trubiani, Thangavelu S. Rajan, Guya D. Marconi, Placido Bramanti, Emanuela Mazzon, Jacopo Pizzicannella, Francesca Diomede
Summary: Tissue engineering has become a major focus of research, with the potential of biomaterials and mesenchymal stem cells (MSCs) showing promise for bone regeneration, particularly in oral bone regeneration. Combining MSCs with biomaterials enhances bone regenerative power, and utilizing the MSC secretome also presents a promising future for bone tissue regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cardiac & Cardiovascular Systems
Michael Nguyen-Truong, Peiman Hematti, Zhijie Wang
Summary: MSCs have been studied for nearly two decades as a therapy for myocardial restoration, with a focus on their paracrine function. Utilizing MSC-derived cell-free approaches for regenerative therapy comes with advantages and disadvantages, and involves mechanisms such as angiogenesis and immunomodulation. Researchers are working towards enhancing these properties to improve therapeutic outcomes, while facing remaining challenges in the field.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Engineering, Biomedical
Yu-Ting Song, Yan-Qing Li, Mao-Xuan Tian, Jun-Gen Hu, Xiu-Ru Zhang, Peng-Cheng Liu, Xiu-Zhen Zhang, Qing-Yi Zhang, Li Zhou, Long-Mei Zhao, Jesse Li-Ling, Hui-Qi Xie
Summary: The demand for bladder reconstruction and the side effects of cystoplasty have led to the development of alternative material substitutes. In this study, an anti-CD29 antibody-conjugated SIS scaffold capable of capturing urine-derived stem cells in situ for tissue repair and regeneration was developed. In vivo experiments showed that the scaffold promoted rapid endothelium healing and smooth muscle regeneration.
BIOACTIVE MATERIALS
(2022)
Review
Cell Biology
Ming-Li Zou, Si-Yu Liu, Zi-Li Sun, Jun-Jie Wu, Zheng-Dong Yuan, Ying-Ying Teng, Yi Feng, Feng-Lai Yuan
Summary: The incidence of acute and chronic wound diseases is increasing, causing a significant burden on patients, their families, and society due to their complex pathogenesis, long course, and difficult treatment. Research is focusing on identifying new ideas and methods for treatment, with adipose stem cells potentially playing a key role in wound healing.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Qiulin He, Junxin Lin, Fanghao Zhou, Dandan Cai, Yiyang Yan, Yejie Shan, Shufang Zhang, Tiefeng Li, Xudong Yao, Hongwei Ouyang
Summary: Nonuniform microstretching (NUMS), a natural phenomenon in bone tissues, promotes osteogenic differentiation and has potential positive effects on bone healing.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Xinyuan Zhao, Xianwen Liu, Li Cui
Article
Medicine, General & Internal
Li Cui, Huan Chen, Xinyuan Zhao
Article
Endocrinology & Metabolism
Li Cui, Xinyuan Zhao, Zhenning Jin, Hailin Wang, Shun-Fa Yang, Shen Hu
Summary: Metabolic remodeling is a hallmark of cancer, and in this study, it was found that melatonin inhibits the folate cycle through downregulation of MTHFD1L, thereby halting HNSCC progression.
JOURNAL OF PINEAL RESEARCH
(2021)
Review
Microbiology
Yi Huang, Xinyuan Zhao, Li Cui, Shaohong Huang
Summary: The oral microbiome is closely related to oral and systemic health, with dental plaque biofilms being the primary factor of periodontitis. Analyzing the composition and functional characteristics of microbial communities is crucial for understanding the pathogenesis of periodontitis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Oncology
Zizhao Mai, Huan Chen, Mingshu Huang, Xinyuan Zhao, Li Cui
Summary: This study identified differentially expressed metabolic enzymes (DEMEs) in head and neck squamous cell carcinoma (HNSCC) tissues and constructed a prognostic signature and nomogram model for predicting the clinical outcome of HNSCC. The results showed that the DEME-based signature can effectively predict the prognosis of HNSCC and the nomogram model can provide personalized therapeutic guidance. In vitro experiments also demonstrated that the knockdown of one of the DEMEs suppressed malignant activities of HNSCC cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Xinyuan Zhao, Huan Chen, Yu Qiu, Li Cui
Summary: The expression of FAM64A is significantly elevated in HNSCC tissues and cell lines, and is strongly associated with an unfavorable prognosis. Depletion of FAM64A suppresses the malignant activities of HNSCC cells both in vitro and in vivo. Mechanistically, FAM64A enhances the transcriptional activity of FOXM1 and modulates its expression through an autoregulation loop. These findings reveal a previously unknown mechanism behind the activation of FOXM1 in HNSCC and suggest that FAM64A could be a promising molecular therapeutic target.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Li Cui, Xiang Wang, Xinyuan Zhao, Bingbing Sun, Tian Xia, Shen Hu
Summary: In this study, a mass spectrometry-based global metabolomic analysis was conducted to investigate the metabolomic changes in BEAS-2B cells exposed to CeO2 nanoparticles (NPs) with different aspect ratios. The study revealed subtle changes in metabolic activities that traditional functional assays might have missed and discovered a previously unknown molecular mechanism underlying CeO2 NP-induced lung fibrosis with different aspect ratios.
Article
Cell & Tissue Engineering
Xinyuan Zhao, Wenjuan Sun, Bing Guo, Li Cui
Summary: This study found that downregulation of circBIRC6 can enhance the osteogenic potential of PDLSCs under inflammatory conditions, and circBIRC6 regulates the osteogenic differentiation of PDLSCs through miR-543. Furthermore, the circBIRC6/miR-543 axis regulates the mineralization capacity of PDLSCs in the inflammatory microenvironment via the PTEN/PI3K/AKT/mTOR signaling pathway. These findings may provide new targets and treatment strategies for bone loss in periodontitis.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Xinyuan Zhao, Dalong Shu, Wenjuan Sun, Shanshan Si, Wei Ran, Bing Guo, Li Cui
Summary: In this study, it was found that PLEK2 expression was significantly increased in HNSCC tissues, and PLEK2 overexpression was strongly associated with poor overall survival and therapeutic resistance. PLEK2 played an important role in maintaining the malignant behaviors of HNSCC cells, altering cellular metabolism, and enhancing c-Myc signaling in HNSCC.
CANCER COMMUNICATIONS
(2022)
Review
Chemistry, Multidisciplinary
Xin Liao, Yudong Liu, Jiarong Zheng, Xinyuan Zhao, Li Cui, Shen Hu, Tian Xia, Shanshan Si
Summary: With the rapid development of engineered nanomaterials (ENMs) in biomedical applications, evaluating their biocompatibility and cytotoxicity has become crucial. Recent studies have shown that inflammasome activation, particularly the NLRP3 inflammasome's interaction with ENMs, plays a significant role in inducing inflammatory responses. Understanding the pathways linking ENMs to NLRP3 inflammasome activation is essential for safer nanomaterial designs and mitigating ENM-induced adverse health effects.
Review
Cell & Tissue Engineering
Ye Lu, Zizhao Mai, Li Cui, Xinyuan Zhao
Summary: Mesenchymal stem cell-based therapy is effective for bone regeneration, but clinical translation has limitations. The secretome of mesenchymal stem cells, especially exosomes, has a critical role in bone repair and regeneration. Exosomes, with their nanosized structures and cargo of proteins, lipids, RNAs, growth factors, and cytokines, are being investigated for their potential in bone regenerative medicine. Preconditioning of parental cells and exosome engineering can enhance their regenerative potential, and biomaterial-assisted exosomes have emerged as a promising strategy for bone regeneration. This review discusses the roles of exosomes in bone regeneration, the use of engineered exosomes and biomaterial-assisted exosomes, and the current challenges in translating exosomes to clinical applications.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Xinyuan Zhao, Zizhao Mai, Ye Lu, Li Cui, Jinhua Yu
Summary: This study found that KLF9 is a crucial transcription factor that controls the osteogenic differentiation of human dental stem cells (DSCs) by negatively regulating the Notch1 signaling pathway.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)
Article
Chemistry, Multidisciplinary
Xinyuan Zhao, Bing Guo, Wenjuan Sun, Jinhua Yu, Li Cui
Summary: Cisplatin resistance in head and neck squamous cell carcinoma (HNSCC) is driven by squalene epoxidase (SQLE), which operates through a cholesterol-dependent pathway. SQLE stabilizes c-Myc protein and prevents its degradation, leading to the activation of c-Myc transcriptional activity. Inhibition of SQLE disrupts the binding of Akt to lipid rafts, deactivates Akt, and destabilizes c-Myc. In a mouse model, SQLE is found to be critical for HNSCC initiation and progression. The combination of terbinafine and cisplatin shows promising synergistic effects in arresting tumor growth. These findings provide insights into SQLE-mediated cisplatin resistance and propose a potential therapeutic avenue for HNSCC patients.
Article
Oncology
Yu Qiu, Li Cui, Yang Lin, Bingju Gao, Jun Li, Xinyuan Zhao, Xiaofeng Zhu, Shen Hu, Lisong Lin
FRONTIERS IN ONCOLOGY
(2020)
