期刊
APPLIED SCIENCES-BASEL
卷 12, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/app12031763
关键词
Curcubita pepo seeds; green extraction techniques; nutraceutical industry; valorization; sustainability
类别
资金
- Fundacao para a Ciencia e a Tecnologia (FCT)/Ministerio da Ciencia, Tecnologia e Ensino Superior (MCTES) through national funds [UIDB/50006/2020, UIDP/50006/2020]
- [SFRH/BD/144994/2019]
- [2020.07519]
- [CEECIND/02702/2017]
- [CEECIND/01886/2020]
There is a growing demand for nutraceuticals to prevent diseases related to redox imbalances. This study aimed to extract value-added compounds from Curcubita pepo seeds using green methodologies and evaluate their antioxidant/antiradical activity. Microwave-assisted extraction (MAE) showed the best performance, particularly MAE condition 2.
Nowadays there is a growing demand for nutraceuticals to prevent diseases related to redox imbalances, such as atherosclerosis and diabetes, being crucial to search for new matrixes rich in bioactive compounds. This work aims to characterize the value-added compounds extracted from Curcubita pepo seeds using green methodologies, namely microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE), employing water as an extracting solvent for two ratios (condition 1: 1 mg/20 mL; condition 2: 2.5 mg/20 mL). The extract with the best antioxidant/antiradical activity in FRAP (71.09 mu mol FSE/g DW) and DPPH (5.08 mg TE/g DW) assays was MAE condition 1, while MAE condition 2 exhibited the highest activity in the ABTS assay (13.29 mg AAE/g DW) and TPC (16.89 mg GAE/g DW). A remarkable scavenging capacity was observed, particularly for HOCl, with IC50 values ranging from 1.88-13.50 mu g/mL. A total of 21 phenolic compounds were identified, being catechin (4.567-7.354 mg/g DW), caffeine (1.147-2.401 mg/g DW) and gallic acid (0.945-1.337 mg/g DW) predominant. No adverse effects were observed on Caco-2 viability after exposure to MAE extracts, while the other conditions led to a slight viability decrease in NSC-34. These results highlighted that the extract from MAE condition 2 is the most promising as a potential nutraceutical ingredient.
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