期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 7, 期 10, 页码 4991-4998出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.1c00734
关键词
tissue sealant; gelatin; dural repair; anti-inflammation; unsaturated fatty acid
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [18J22422, 20H02470]
- Project for Translational Research program
- Strategic Promotion for Practical Application of Innovative Medical Technology from Japan Agency of Medical Research and Development (AMED) [JP20lm0203114h0001, JP20lm0203010]
- Grants-in-Aid for Scientific Research [18J22422] Funding Source: KAKEN
A fish gelatin-based anti-inflammatory sealant was developed to prevent cerebrospinal fluid leakage during craniotomy. The sealant showed lower swelling ratio, similar burst strength, and higher anti-inflammatory properties compared to a commercially available dural sealant.
Cerebrospinal fluid (CSF) leakage from the dura mater during craniotomy is a common complication, which is associated with infection, meningitis, pneumocephalus, and delayed wound healing. In the present study, we developed an absorbable fish gelatin-based anti-inflammatory sealant for dura mater sealing to prevent CSF leakage. Gelatin derived from Alaska pollock (ApGltn) was modified with alpha-linolenic acid (ALA), an omega-3 fatty acid that exhibits anti-inflammatory properties, and cross-linked with a poly(ethylene glycol)-based cross-linker to develop ALA-ApGltn sealant (ALA-Seal). ALA-Seal demonstrated a higher storage modulus and tangent delta (tan delta) compared with those of the original ApGltn sealant (Org-Seal). The swelling ratio of ALA-Seal was markedly lower than that of DuraSeal, a commercially available dural sealant. Ex vivo burst strength measurements using porcine dura mater indicated that there was no significant difference between DuraSeal and ALA-Seal, despite ALA-Seal having an order of magnitude lower storage modulus. The anti-inflammatory properties of ALA-Seal, evaluated using brain microglial cells, were considerably higher than those of DuraSeal and Org-Seal, with a minimal adverse effect on cell viability. Therefore, compared to DuraSeal, ALA-Seal is a potential dural sealant with a lower swelling ratio, similar burst strength, and higher anti-inflammatory properties, which may prevent CSF leakage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据