Article
Polymer Science
Ahmed Fatimi, Oseweuba Valentine Okoro, Daria Podstawczyk, Julia Siminska-Stanny, Amin Shavandi
Summary: Three-dimensional printing is considered an important technology in tissue engineering, especially due to the growing demand for organ replacement and tissue regeneration. In 3D bioprinting, the ink used is impregnated with cells, allowing for immediate scaffold cellularization and generation of complex structures. This study comprehensively explores the use of bio-inks based on natural polymers in 3D bioprinting, discussing progress, techniques and approaches, as well as limitations and future trends.
Article
Polymer Science
Jonghyeuk Han, Wonwoo Jeong, Min-Kyeong Kim, Sang-Hyeon Nam, Eui-Kyun Park, Hyun-Wook Kang
Summary: A bio-ink based on demineralized dentin matrix (DDM) particles with enhanced 3D printability was developed for engineering dental tissues. The higher the DDM particle concentration, the better the printability and cell compatibility of the bio-ink, leading to improved printing resolution and stacking ability. The study demonstrated the successful fabrication of cellular constructs with 3D patient-specific shapes and clinically relevant sizes through co-printing with polycaprolactone and DDM particle-laden bio-ink.
Review
Engineering, Biomedical
Lan Lin, Songli Jiang, Jun Yang, Jiandi Qiu, Xiaoyi Jiao, Xusong Yue, Xiurong Ke, Guojing Yang, Lei Zhang
Summary: This review focuses on the application of nanocellulose and cellulose derivatives in 3D bioprinting of bone and cartilage. Cellulose is a common bioprintable material with biocompatibility, biodegradability, low cost, and printability. However, the potential applications of nanocellulose and cellulose derivative-based bio-inks have not been fully explored.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Meik Neufurth, Shunfeng Wang, Heinz C. Schroeder, Bilal Al-Nawas, Xiaohong Wang, Werner E. G. Mueller
Summary: Three-dimensional printing processes are recognized as important fabrication techniques in tissue engineering. This study proposes a bio-ink formulation enriched with polyphosphate (polyP) to increase the viability and migration propensity of mesenchymal stem cells (MSC). The bio-ink also stimulates the growth and differentiation of MSC to mineral depositing osteoblasts.
Article
Biotechnology & Applied Microbiology
Zhiyu Ding, Ning Tang, Junjie Huang, Xu Cao, Song Wu
Summary: This study analyzed 3,327 published articles on 3D bioprinting using VOSviewer, CiteSpace, and R-bibliometrix. The results showed that the number of annual publications is increasing globally, and the United States and China are the most active countries in this field. The research focus includes bio-ink, hydrogels, scaffold, extrusion-based bioprinting, tissue engineering, and in vitro models. Future research hotspots predicted are new bio-ink investigation, modification of extrusion-based bioprinting for cell viability and vascularization, application of 3D bioprinting in organoids and in vitro models, and research in personalized and regenerative medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Polymer Science
Xing Huang, Yuxi Zhang, Mengquan Shi, Li-Peng Zhang, Yunlong Zhang, Yuxia Zhao
Summary: A highly biocompatible bio-ink containing a hydrophilic photoinitiator and modified gelatin was used to fabricate 3D hydrogel scaffolds with mouse fibroblast cells via two-photon polymerization. The cells showed vigorous proliferation within the scaffolds after four days of incubation, suggesting great potential for 3D bioprinting.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Cell & Tissue Engineering
Wonwoo Jeong, Min Kyeong Kim, Hyun-Wook Kang
Summary: The type of detergent used for decellularization has a significant impact on the performance of decellularized extracellular matrix-based bio-inks, with TXA-based bio-ink showing the highest ECM content and best overall performance, including improved gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. Cytocompatibility tests using primary mouse hepatocytes also demonstrated that the TXA-based dECM bio-ink led to increased albumin secretion and cytochrome P450 activity compared to other bio-inks.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Engineering, Biomedical
Hanjun Hwangbo, Hyeongjin Lee, Eun-Ju Jin, JaeYoon Lee, Yunju Jo, Dongryeol Ryu, GeunHyung Kim
Summary: This study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking strategy. The optimal biophysical properties of the muscle construct were achieved by evaluating UV exposure sources, exposure dose, and wall shear stress. The ISC system showed higher uniaxial alignment and myogenesis compared to the conventional crosslinking strategy. Muscle constructs laden with human adipose stem cells showed significant restoration of functionality and muscular volume in vivo.
BIOACTIVE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Jiaqi Xu, Manyue Zhang, Wenzhen Du, Jiuhong Zhao, Guixia Ling, Peng Zhang
Summary: The emerging 3D bioprinting technology has brought hope for the development of tissue engineering and regenerative medicine. Chitosan-based supramolecular hydrogels are considered as ideal materials for 3D bioprinting. However, the mechanical properties of these hydrogels still require further research and exploration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Manufacturing
Md Ahasan Habib, Bashir Khoda
Summary: 3D bio-printing is an emerging technology that can fabricate tissue scaffold in-vitro. This paper demonstrates a roadmap to develop and optimize bio-inks, ensuring printability, shape fidelity, and cell survivability. The results show that the designed bio-ink can sustain high extrusion pressure and maintain cell viability, making it suitable for 3D bio-printing process.
JOURNAL OF MANUFACTURING PROCESSES
(2022)
Article
Chemistry, Multidisciplinary
Jinchang Zhu, Yi He, Linlin Kong, Zhijian He, Kaylen Y. Kang, Shannon P. Grady, Leander Q. Nguyen, Dong Chen, Yong Wang, Jose Oberholzer, Li-Heng Cai
Summary: A voxelated bioprinting technique, DASP, has been developed for the digital assembly of spherical particles, enabling the generation and assembly of bio-ink droplets. Using DASP, mechanically robust porous scaffolds have been created for encapsulating human pancreatic islets to achieve sustained insulin release. The study shows the potential for engineering tissue constructs with highly heterogeneous yet tightly organized structures for therapeutic applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Respiratory System
Mohammadhossein Dabaghi, Mabel Barreiro Carpio, Jose Manuel Moran-Mirabal, Jeremy Alexander Hirota
Summary: The field of 3D bioprinting is advancing rapidly, and efforts are being made to replicate the human lung using interdisciplinary advancements in 3D printing technology, bioink formulations, and cell biology.
EUROPEAN RESPIRATORY JOURNAL
(2023)
Review
Engineering, Biomedical
Yanshen Yang, Yuanbo Jia, Qingzhen Yang, Feng Xu
Summary: Three-dimensional bioprinting is a promising approach to construct functional biomimetic tissues. Bio-inks, which are essential for cell microenvironment construction, are influenced by mechanical properties. With the advancement of functional biomaterials, engineered bio-inks have allowed the creation of cell mechanical microenvironments in vitro.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Engineering, Biomedical
Cesar R. Alcala-Orozco, Xiaolin Cui, Gary J. Hooper, Khoon S. Lim, Tim B. F. Woodfield
Summary: This review examines the application of hybrid biofabrication strategies in skeletal regeneration and introduces a classification system to assess the advantages, challenges, and potential applications of various strategies.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Multidisciplinary
Pei Zhuang, Zachary Greenberg, Mei He
Summary: A novel 3D printable nanocomposite starch hydrogel was developed with enhanced biocompatibility for promoting 3D cell growth, utilizing gelatin nanoparticles and collagen. The unique rheological properties of the hydrogel, along with the homogeneous microporous structure containing collagen fibers and gelatin nanoparticles, support cell attachment and proliferation. The cell metabolic activity study confirmed the remarkable enhancement of biological function of the developed starch hydrogel, making it a highly desirable bio-ink for advancing 3D tissue engineering.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Review
Immunology
Paulina Niedzwiedzka-Rystwej, Mikolaj Wolacewicz, Piotr Cywoniuk, Marta Klak, Michal Wszola
ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS
(2020)
Article
Multidisciplinary Sciences
M. Klak, M. Gomolka, T. Dobrzanski, G. Tymicki, P. Cywoniuk, P. Kowalska, K. Kosowska, T. Bryniarski, A. Berman, A. Dobrzyn, J. Idaszek, W. swieszkowski, M. Wszola
Review
Chemistry, Analytical
Marta Klak, Tomasz Bryniarski, Patrycja Kowalska, Magdalena Gomolka, Grzegorz Tymicki, Katarzyna Kosowska, Piotr Cywoniuk, Tomasz Dobrzanski, Pawel Turowski, Michal Wszola
Article
Chemistry, Analytical
Marta Klak, Patrycja Kowalska, Tomasz Dobrzanski, Grzegorz Tymicki, Piotr Cywoniuk, Magdalena Gomolka, Katarzyna Kosowska, Tomasz Bryniarski, Andrzej Berman, Agnieszka Dobrzyn, Wojciech Sadowski, Bartosz Gorecki, Michal Wszola
Summary: This study assessed the impact of 3D bioprinting process on islet viability, showing that optimal pressure for printing pancreatic islets should be lower than 30 kPa when using 3% alginate as a carrier. This finding highlights the importance of pressure control in achieving high viability of islets during 3D bioprinting.
Article
Biochemistry & Molecular Biology
Marta Klak, Ilona Lojszczyk, Andrzej Berman, Grzegorz Tymicki, Anna Adamiok-Ostrowska, Maciej Sierakowski, Radoslaw Olkowski, Andrzej Antoni Szczepankiewicz, Artur Kaminski, Agnieszka Dobrzyn, Michal Wszola
Summary: This study aimed to assess the safety of decellularized porcine pancreas and analyze the risk of an undesirable immune response. Through testing different variants of the decellularization process, the most favorable variant in terms of quality and biology was chosen, demonstrating an effective and safe method for the development of transplant and regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Michal Wszola, Daria Nitarska, Piotr Cywoniuk, Magdalena Gomolka, Marta Klak
Summary: Type 1 diabetes is a common autoimmune disease that can be treated by culturing human stem cells to differentiate into insulin and glucagon-producing cells for the creation of a bionic pancreas. Solutions for post-transplant immune responses triggered by stem cell-derived pancreatic cells need further exploration.
Article
Biochemistry & Molecular Biology
Michal Wszola, Marta Klak, Anna Kosowska, Grzegorz Tymicki, Andrzej Berman, Anna Adamiok-Ostrowska, Joanna Olkowska-Truchanowicz, Izabela Uhrynowska-Tyszkiewicz, Artur Kaminski
Summary: The study investigated the use of streptozotocin (STZ) to induce diabetes in mice, leading to over 90% destruction of beta-cell mass. However, STZ doses below 300 mg/kg did not show significant changes in islet area and insulin-positive areas in mice.
Review
Immunology
Marta Klak, Magdalena Gomolka, Patrycja Kowalska, Justyna Cichon, Filip Ambrozkiewicz, Marta Serwanska-Swietek, Andrzej Berman, Michal Wszola
CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY
(2020)
