Article
Food Science & Technology
Jiali Wang, Chengshun Lu, Qiang Xu, Zhongyuan Li, Yajian Song, Sa Zhou, Le Guo, Tongcun Zhang, Xuegang Luo
Summary: The genome sequencing analysis of strain LTJ12 revealed it to be a potentially safe probiotic with high ethanol tolerance and alcohol metabolism. It belongs to the L. pentosus species. This study provides valuable insights into the accurate identification and rational application of Lactiplantibacillus species.
Article
Biochemistry & Molecular Biology
Konstantinos Tegopoulos, Odysseas Sotirios Stergiou, Despoina Eugenia Kiousi, Margaritis Tsifintaris, Ellie Koletsou, Aristotelis C. Papageorgiou, Anthoula A. Argyri, Nikos Chorianopoulos, Alex Galanis, Petros Kolovos
Summary: Lactiplantibacillus plantarum is a diverse species with potential probiotic traits, as shown by the genomic analysis and functional studies of strain L125.
Article
Food Science & Technology
Clinton A. Page, Ilenys M. Perez-Diaz, Meichen Pan, Rodolphe Barrangou
Summary: The study conducted a comparative genomic analysis on different Lactiplantibacillus strains in cucumber fermentation and identified distinct features between L. pentosus and L. plantarum. The study also revealed the diversity of L. pentosus in the fermentation habitat. These findings are important for the selection of functional starter cultures.
Article
Biotechnology & Applied Microbiology
Francesco M. Carpi, Maria Magdalena Coman, Stefania Silvi, Matteo Picciolini, Maria Cristina Verdenelli, Valerio Napolioni
Summary: This study aimed to refine the taxonomy and functional characterization of Lactiplantibacillus plantarum genomes through pan-genome analysis, focusing on probiotic potential. The comprehensive genomic analysis of 127 L. plantarum strains provided new insights into the genomic content and variability, establishing a solid reference panel for future characterization of probiotic strains. The study highlighted unique features of L. plantarum strains and identified approximately 70% of probiotic marker genes belonging to the core/soft-core genome.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Han-Yin Hsu, Yi-Chu Liao, Shih-Hsuan Lin, Jin-Seng Lin, Chia-Chia Lee, Koichi Watanabe
Summary: In this study, the safety of TWK10 was comprehensively assessed both in vivo and in vitro. The results showed that TWK10 did not cause genetic mutations, chromosomal abnormalities, red blood cell abnormalities, or subacute toxicity. Additionally, TWK10 was susceptible to all antibiotics tested, except kanamycin, and no antimicrobial resistance genes, virulence factors, or genes involved in biogenic amine synthesis were found in its genome.
Article
Food Science & Technology
Elio Lopez-Garcia, Antonio Benitez-Cabello, Javier Ramiro-Garcia, Victor Ladero, Francisco Noe Arroyo-Lopez
Summary: Lactiplantibacillus pentosus LPG1, a probiotic bacteria obtained from plant origins, was sequenced and analyzed. The results showed that it is a safe microorganism with potential as a human probiotic for vegetable fermentations.
Article
Food Science & Technology
Dong-Mei Liu, Yan-Yan Huang, Ming-Hua Liang
Summary: This study explored the probiotic properties and adaptive responses of Lactiplantibacillus plantarum DMDL 9010(LP9010) isolated from Chinese traditional fermented paocai. LP9010 showed good tolerance to gastrointestinal environment and exhibited various probiotic functions. Comparative genomic analysis identified unique genes and stress-related proteins in LP9010.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hongbiao Li, Ting Liu, Xiuqin Zhang, Zhiguo Xiong, Qing Hong, Shuang Jia, Yi Lin, Liping Wang, Yueliang Zhao
Summary: This study used genomic tools to characterize the probiotic and bactericidal potential of Lactiplantibacillus plantarum HY41. It was found that HY41 carried multiple functional genes and a putative bacteriocin biosynthetic cluster. A silent gene cluster responsible for producing a potent bacteriocin was also identified in HY41. The mode of action of the bacteriocin was studied and it was found to cause cell death by disrupting the cell membrane of Listeria monocytogenes. These findings confirm the probiotic and bactericidal properties of HY41 and demonstrate its potential for industrial probiotic applications.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Microbiology
Jie Sheng, Rory Cave, Mary M. Ter-Stepanyan, Nune Kotsinyan, Jiazhen Chen, Li Zhang, Taijiao Jiang, Hermine V. Mkrtchyan
Summary: This study reports the first whole-genome sequencing of a multidrug-resistant Klebsiella pneumoniae isolate from Armenia. The isolate exhibited resistance to multiple antibiotics and carried several antibiotic resistance genes. The study emphasizes the importance of genomic surveillance and implementation of infection prevention and control measures for emerging pathogens.
MICROBIOLOGY SPECTRUM
(2023)
Article
Genetics & Heredity
Honghong Dong, Ruixiang Gao, Yijie Dong, Qing Yao, Honghui Zhu
Summary: This study presented the high-quality genome assembly of Myxococcus xanthus R31, a strain with good biocontrol efficacy against tomato bacterial wilt. Comparative genomics analysis revealed the phylogenetic relationship between R31 and other M. xanthus strains, and identified strain-specific genes that may contribute to its predatory ability. These findings provide new insights into R31 and related species, and lay the foundation for further genetic studies on the predation mechanism of myxobacteria.
Article
Microbiology
Xuefang Zhao, Qi Liang, Xuemei Song, Yan Zhang
Summary: This study analyzed the genomic characteristics of Lactiplantibacillus plantarum MC5 and its exopolysaccharide (EPS) biosynthesis mechanism. The results showed that MC5 can metabolize seven sugars and produce a significant amount of EPS. These findings can be used to enhance EPS production through genetic engineering.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Fisheries
Libao Wang, Hui Li, Wenjun Shi, Yi Qiao, Pan Wang, Zhijun Yu, Ran Zhao, Runhao Hu, Hui Shen, Jie Cheng, Ge Jiang, Xintong Huang, Xihe Wan
Summary: In this research, the whole genome of Enterocytozoon hepatopenaei (EHP), an obligate intracellular parasite of shrimp, was sequenced, and detailed functional annotation was conducted. Comparative genomic analysis revealed the evolutionary features of EHP, including gene family clustering, phylogenetic relationships, gene family expansion and contraction, and synteny. These findings deepen our understanding of the EHP genome.
AQUACULTURE INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Esther Alonso Garcia, Nabil Benomar, Leyre Lavilla Lerma, Juan Jose de la Fuente Ordonez, Charles W. Knapp, Hikmate Abriouel
Summary: A study found that pre-adapting Lactiplantibacillus pentosus strains to vegetable-based edible oils significantly changed their susceptibility to antibiotics and induced gene expressions in some stress responses. This may provide a new approach for manufacturing probiotic products with improved stability, functionality, and robustness.
Article
Microbiology
Esther Alonso Garcia, Juan Jose de la Fuente Ordonez, Leyre Lavilla Lerma, Maria D. Estudillo-Martinez, Sonia Castillo-Gutierrez, Nabil Benomar, Charles W. Knapp, Hikmate Abriouel
Summary: This study found that pre-adapting Lactiplantibacillus pentosus strains to vegetable-based edible oils can enhance their probiotic functionality, with olive oil adaptation showing improved stability and functionality. Transcriptomic analysis revealed that the adaptation to olive oil led to rerouting of metabolic pathways and regulation of genes related to energy production, cell growth, host interactions, and other physiological features, suggesting a novel approach for designing probiotic products with enhanced stability and functionality.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agricultural Engineering
Bingchao Wu, Jie Zhu, Xixi Ma, Jiyuan Jia, Dan Luo, Qiong Ding, Xiaoshan Wang, Linkai Huang
Summary: By comparing the chloroplast genomes of upland and lowland ecotypes of switchgrass, this study reveals conservation in terms of repetitive sequences, codon usage, and the identification of a lowland-specific hotspot. The phylogenetic analysis also highlights the complexity of the evolutionary process of switchgrass.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Environmental Sciences
Christos Eliopoulos, Dimitrios Arapoglou, Nikos Chorianopoulos, Giorgos Markou, Serkos A. Haroutounian
Summary: This study focuses on utilizing solid state fermentation to improve the nutritional value of brewers' spent grain (BSG) for animal feed. By using edible fungi Pleurotus ostreatus, significant increases in protein content, beta-glucans, and reduction in cellulose were achieved through SSF. This method can potentially reduce environmental impact from beer brewing while producing animal feed with higher protein content and improved nutritional characteristics.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Microbiology
Despoina Eugenia Kiousi, Nikos Chorianopoulos, Chrysoula C. Tassou, Alex Galanis
Summary: Food fermentation has improved the safety and organoleptic characteristics of raw materials, and the potential health benefits of these products have attracted scientific attention. However, only a few products have been authorized as 'functional foods' due to data inconsistency and lack of in-depth preclinical characterization. Omics platforms and bioinformatic algorithms offer comprehensive data analysis of microbe-microbe, microbe-matrix, and microbe-host interactions, providing insights for maximizing their beneficial effects. Integrating these platforms into food science remains a challenge, but coordinated efforts and interdisciplinary collaboration can drive the field towards a new era.
Article
Microbiology
Christina S. Kamarinou, Olga S. Papadopoulou, Agapi Doulgeraki, Chrysoula C. Tassou, Alex Galanis, Nikos G. Chorianopoulos, Anthoula A. Argyri
Summary: The aim of this study was to isolate indigenous lactic acid bacteria from traditional Greek cheeses and evaluate their characteristics. Promising results were obtained, with some strains showing favorable technological and functional properties, contributing to the sensory characteristics and safety of yogurt.
Article
Pediatrics
Kim A. A. Schilders, Gabriela G. Edel, Evelien Eenjes, Bianca Oresta, Judith Birkhoff, Anne Boerema-de Munck, Marjon Buscop-van Kempen, Panagiotis Liakopoulos, Petros Kolovos, Jeroen A. A. Demmers, Raymond Poot, Rene M. H. Wijnen, Dick Tibboel, Robbert J. Rottier
Summary: This study identified proteins associated with SOX2 and showed their potential involvement in co-regulation of lung epithelial cell differentiation.
FRONTIERS IN PEDIATRICS
(2022)
Article
Food Science & Technology
Patra Sourri, Chrysoula C. Tassou, George-John E. Nychas, Efstathios Z. Panagou
Summary: Fruit juices are important in a healthy diet, but thermo-acidophilic endospore forming bacteria, such as Alicyclobacillus, can cause spoilage. This review discusses various chemical, physical, and non-thermal treatments to control Alicyclobacillus growth and ensure the quality and shelf life of fruit juices.
Article
Chemistry, Multidisciplinary
Ioanna Prapa, Anastasios Nikolaou, Panayiotis Panas, Chrysoula Tassou, Yiannis Kourkoutas
Summary: This study focuses on the design and development of ready-to-use dry food ingredients that contain a high number of functional microbes, which can be directly added to food systems. The aim is to create stable freeze-dried functional food ingredients with enhanced shelf-life during long storage. The technology developed in this study has important advantages for maintaining cell viability during storage and ensuring stability of ready-to-use functional food ingredients.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Katerina Spyridopoulou, Sotiris Kyriakou, Angeliki Nomikou, Angelos Roupas, Antreas Ermogenous, Katerina Karamanoli, Daniela Moyankova, Dimitar Djilianov, Alex Galanis, Mihalis I. Panayiotidis, Aglaia Pappa
Summary: This study extracted aqueous and ethanolic extracts from the leaves of the Balkan endemic plant Haberlea rhodopensis, finding that the ethanolic extract exhibited stronger inhibitory effects on human cancer cells and contained more antioxidant compounds, demonstrating potential anticancer properties.
Article
Microbiology
Despoina Eugenia Kiousi, Joanna Bucka-Kolendo, Adrian Wojtczak, Barbara Sokolowska, Agapi I. I. Doulgeraki, Alex Galanis
Summary: This study presents the annotated whole genome sequence of two strains of Loigolactobacillus backii, a beer-spoiling bacteria with high hop tolerance. The genetic basis and adaptation of the strains in the brewery environment were studied, revealing their ability to survive and grow in the presence of hop compounds. The findings provide insights into the mechanisms behind the tolerance of Lg. backii to extreme conditions in the brewery.
Article
Immunology
Despoina Eugenia Kiousi, Christos Efstathiou, Vasilis Tzampazlis, Stavros Plessas, Maria Panopoulou, Maria Koffa, Alex Galanis
Summary: This study examined the antimicrobial and antibiofilm properties of Lactiplantibacillus pentosus L33, Lactiplantibacillus plantarum L125, and Lacticaseibacillus paracasei SP5 against clinical isolates of Staphylococcus aureus, Salmonella enterica, and Escherichia coli. The results showed that these three lactobacilli were able to inhibit the growth and biofilm formation of the pathogens. Gene expression analysis and sequence prediction also revealed their ability to produce functional bacteriocins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Oncology
Despoina E. Kiousi, Antonia Z. Kouroutzidou, Konstantinos Neanidis, Emmanuel Karavanis, Dimitrios Matthaios, Aglaia Pappa, Alex Galanis
Summary: Cancer immunotherapy aims to stimulate the immune response against tumors, and the gut microbiota plays a crucial role in immunity and cancer immunotherapy. Manipulating the gut microbiota through dietary interventions or fecal microbiota transplantation can enhance the efficacy of immunotherapy and lead to personalized treatments.
Article
Microbiology
Agapi I. Doulgeraki, Christina S. Kamarinou, George-John E. Nychas, Anthoula A. Argyri, Chrysoula C. Tassou, Georgios Moulas, Nikos Chorianopoulos
Summary: Microbial interactions have a significant impact on biofilm formation and the efficacy of disinfectants. This study assessed the effect of microbial interactions on biofilm formation and the disinfection activity of a photocatalytic surfactant based on TiO2 nanoparticles. The presence of different species or dual-species biofilms influenced the microbial load and population, while the disinfectant enhanced the antimicrobial activity of UV light. The presence of multiple species also affected the resistance of biofilm cells to UV light and disinfectants. These findings highlight the importance of microbial interactions in biofilm formation and decontamination.
Article
Genetics & Heredity
Joanna Bucka-Kolendo, Despoina Eugenia Kiousi, Adrian Wojtczak, Agapi I. Doulgeraki, Alex Galanis, Barbara Sokolowska
Summary: Among beer-spoiling microorganisms, dominant species include Lactobacillus, Leuconostoc, Oenococcus, and Pediococcus. High hydrostatic pressure can eliminate these microorganisms while preserving desired beer properties. This study analyzed the resistance of a beer-spoiling Lactiplantibacillus plantarum strain to hop and high hydrostatic pressure, and confirmed its genome characteristics through sequencing. The findings suggest the strain's growth is affected by beer concentration and it possesses genes for stress resistance and adaptation to the brewing environment.
Article
Microbiology
Despoina E. Kiousi, Dimitrios M. Karadedos, Anastasia Sykoudi, Panagiotis Repanas, Christina S. Kamarinou, Anthoula A. Argyri, Alex Galanis
Summary: A multiplex PCR assay was developed for the detection of potential probiotic lactic acid bacteria strains in yogurt samples, showing its biotechnological applicability and potential for wider use.
Article
Biotechnology & Applied Microbiology
Patra Sourri, Anthoula A. Argyri, George-John E. Nychas, Chrysoula C. Tassou, Efstathios Z. Panagou
Summary: The population dynamics of A. acidoterrestris spores and vegetative cells in orange juice, treated with temperature-assisted HHP and stored in different isothermal conditions, were investigated in this study. The results showed that HHP treatment effectively reduced the spore population, and there were minimal changes in the quantity of spores and vegetative cells during storage, especially at low temperatures.
FERMENTATION-BASEL
(2022)
