Article
Microbiology
Francesca Sivori, Ilaria Cavallo, Daniela Kovacs, Maria Guembe, Isabella Sperduti, Mauro Truglio, Martina Pasqua, Grazia Prignano, Arianna Mastrofrancesco, Luigi Toma, Fulvia Pimpinelli, Aldo Morrone, Fabrizio Ensoli, Enea Gino Di Domenico
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) has become the leading cause of skin and soft tissue infections (SSTIs) and biofilm formation contributes to increased antibiotic tolerance. This study demonstrates that dalbavancin is the most effective antibiotic against MRSA biofilms and extracellular DNA affects its activity. The findings provide new insights for developing targeted therapeutic strategies against MRSA.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Sofia Maraki, Viktoria Eirini Mavromanolaki, Dimitra Stafylaki, Evangelia Iliaki-Giannakoudaki, George Hamilos
Summary: Skin and soft tissue infections (SSTIs) caused by methicillin-resistant Staphylococcus aureus (MRSA) are associated with significant morbidity and healthcare costs. Vancomycin is the preferred treatment for complicated SSTIs caused by MRSA, but newer antibiotics like ceftobiprole, dalbavancin, and tedizolid have shown promise. In this study, we evaluated the in vitro activity of these antibiotics against MRSA clinical isolates and found that dalbavancin had the lowest MIC90, followed by tedizolid, linezolid, ceftobiprole, and daptomycin. Tedizolid exhibited superior in vitro activity compared to other antibiotics. Multidrug-resistant phenotypes were detected in the majority of isolates. The findings suggest that ceftobiprole, dalbavancin, and tedizolid are effective antimicrobials for MRSA-related SSTIs.
Article
Immunology
Sara Alosaimy, Kyle P. Murray, Evan J. Zasowski, Taylor Morrisette, Abdalhamid M. Lagnf, Thomas P. Lodise, Michael J. Rybak
Summary: This study aimed to evaluate the association between vancomycin AUC and clinical outcomes in MRSA cSSTIs, with results showing that achieving target AUC was significantly associated with timely clinical success (TCS), particularly important for high-risk patients.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Elke S. Bergmann-Leitner, Eugene V. Millar, Elizabeth H. Duncan, David R. Tribble, Patrick M. Carey, Michael W. Ellis, Katrin Mende, Jason W. Bennett, Sidhartha Chaudhury
Summary: This study demonstrates the power of immunoprofiling for identifying predictive factors of the pre-illness state of SSTI, thus identifying early stages of infection and individuals susceptible to SSTI.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Infectious Diseases
Matthew S. Linz, Arun Mattappallil, Diana Finkel, Dane Parker
Summary: The most common pathogen in skin-and-soft-tissue infections (SSTIs) in the United States is the pathogenic bacterium Staphylococcus aureus. The epidemic clone USA300 is responsible for the majority of S. aureus SSTIs in the USA. These infections can be serious, with a global age-standardized mortality rate of 0.5 in 2019. S. aureus SSTIs can present as superficial infections with local symptoms or as monomicrobial necrotizing fasciitis, which can cause systemic manifestations and potentially lead to complications or death. Treatment for S. aureus SSTIs usually involves oral therapy, with severe cases requiring parenteral therapy. Treatment options range from cephalosporins and penicillin agents such as oxacillin for methicillin-sensitive S. aureus (MSSA), to vancomycin for methicillin-resistant S. aureus (MRSA). Treatment challenges include adverse effects, risk for Clostridioides difficile infection, and antibiotic resistance.
Article
Integrative & Complementary Medicine
P. A. Akinduti, V Emoh-Robinson, H. F. Obamoh-Triumphant, Y. D. Obafemi, T. T. Banjo
Summary: The antibacterial activities of aqueous extracts from Azadirachta indica and Moringa oleifera were found to be effective against multidrug resistance Staphylococcus aureus associated with skin and soft tissue infections. Other extracts also showed some inhibitory effects.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2022)
Review
Infectious Diseases
Florencia Mariani, Estela Maria Galvan
Summary: Polymicrobial biofilms create a complex environment where different microorganisms can interact in various ways and alter the outcome of diseases. This review article explores the microorganisms commonly found together with Staphylococcus aureus in skin and soft tissue infections, such as Pseudomonas aeruginosa, Escherichia coli, Enterococcus spp., Acinetobacter baumannii, and Candida albicans. The molecular mechanisms and impact of these polymicrobial interactions on pathogenesis and antimicrobial susceptibility are also discussed.
Article
Infectious Diseases
Cristina El Haj, Eva Benavent, Yanik Sierra, Laura Soldevila, Raul Rigo-Bonnin, Benjamin Torrejon, Joan Gomez-Junyent, Irantzu Rossello, Oscar Murillo
Summary: The anti-biofilm efficacy of dalbavancin (DAL) was significantly improved by adding rifampicin (RIF) in the treatment of biofilm-embedded methicillin-resistant Staphylococcus aureus (MRSA). However, the development of RIF resistance in all combination therapies decreased their effectiveness over time.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Review
Infectious Diseases
Joya-Rita Hindy, Sara F. Haddad, Souha S. Kanj
Summary: This article reviews several new drugs for MRSA SSTI treatment and discusses their advantages and disadvantages. However, current IDSA guidelines for MRSA infection and SSTIs, as well as the recently published UK guidelines on MRSA treatment, only consider these drugs as alternative choices or do not mention them at all.
CURRENT OPINION IN INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Pedro L. Gonzalez, Urania Rappo, Karthik Akinapelli, Jennifer S. McGregor, Sailaja Puttagunta, Michael W. Dunne
Summary: All 39 patients with uncomplicated S. aureus bacteremia treated with dalbavancin (single- or two-dose regimen) had clearance of their bloodstream infection. Clinical response rates were similar to daily comparator therapy for 10-14 days.
INFECTIOUS DISEASES AND THERAPY
(2022)
Review
Immunology
Chih-Ming Yueh, Hsin Chi, Nan-Chang Chiu, Fu-Yuan Huang, Daniel Tsung-Ning Huang, Lung Chang, Yen-Hsin Kung, Ching-Ying Huang
Summary: The purpose of this study was to describe the characteristics and outcomes of children hospitalized with skin and soft tissue infections (SSTIs), and to determine if certain clinical and laboratory variables at admission could differentiate between community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) and community-acquired methicillin-sensitive S. aureus (CA-MSSA). The study found that the age group 0-1 month and local lymph node reaction were independent factors for differentiating between CA-MRSA and CA-MSSA infections.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Immunology
Abiola Senok, Stefan Monecke, Rania Nassar, Handan Celiloglu, Sreeraj Thyagarajan, Elke Mueller, Ralf Ehricht
Summary: A study conducted in Dubai evaluated a novel lateral flow immunoassay for PVL detection in Staphylococcus aureus cultures, showing excellent diagnostic accuracy indices. The high occurrence of pvl and fusC genes in MRSA strains causing SSTI underscores the need for constant surveillance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Mar de Pablo-Miro, Sergi Pujol-Ruiz, Simona Iftimie, Maria del Mar Arenas-Miras, Inmaculada Lopez-Montesinos, Xavier Duran-Jorda, Albert Angles, Santiago Grau, Juan P. Horcajada
Summary: Dalbavancin, a new antibiotic effective against Gram-positive microorganisms, offers the possibility of weekly IV therapy in outpatient setting. A case-control study showed that Dalbavancin treatment reduced hospital stay and improved 30-day efficacy compared to other antibiotics.
Article
Infectious Diseases
Carolina Ferreira, Sofia Santos Costa, Maria Serrano, Ketlyn Oliveira, Graca Trigueiro, Constanca Pomba, Isabel Couto
Summary: This study analyzed a collection of 34 S. aureus isolates from ambulatory patients in Portugal, and found that nearly half of the isolates were MRSA and/or MDR. The isolates showed high antimicrobial resistance and genetic diversity, with prevalence of clonal lineages CC5, CC22, and CC8, and PVL-encoding genes were found in 20.6% of the isolates.
Article
Infectious Diseases
Sebastian Simon, Bernhard J. H. Frank, Susana Hartmann, Laetitia Hinterhuber, Michael Reitsamer, Alexander Aichmair, Martin Dominkus, Bo Soderquist, Jochen G. Hofstaetter
Summary: This study compared the treatment outcome of dalbavancin with Standard of Care (SoC) in hip and knee periprosthetic joint infections (PJIs). The results showed that dalbavancin treatment resulted in a similar outcome to SoC, with excellent safety and low rate of adverse effects.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Dermatology
Aldo Morrone, Maria Lucia Dell'Anna, Antonio Cristaudo, Tewolde Wubayehu, Hagos Godefay, Gebre Ab Barnabas, Federica Dassoni, Valeska Padovese, Ottavio Latini, Torello Lotti, Pierluigi Saraceni, Enea Gino Di Domenico, Viviana Lora
Summary: This study conducted in Tigray, Ethiopia, found that plaque psoriasis is the most common form, with a 17% prevalence of psoriatic arthritis. In poorer rural areas, patients use traditional African plants for treatment. Further research on appropriate management and reducing costs of conventional drugs in low-income countries could benefit psoriasis care.
DERMATOLOGIC THERAPY
(2022)
Article
Cardiac & Cardiovascular Systems
Stefano Di Bella, Giuseppina Campisciano, Roberto Luzzati, Enea Gino Di Domenico, Antonio Lovecchio, Aniello Pappalardo, Manola Comar, Giuseppe Gatti
Summary: The study found bacterial DNA sequences in heart valve tissues of patients without infective endocarditis, with Escherichia coli being the most commonly identified bacterium. There was a higher rate of bacterial DNA positivity in calcific valve tissues, suggesting an association between the valvular microbiome and valve degeneration models and metabolic disorders.
INTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY
(2021)
Article
Oncology
Fulvia Pimpinelli, Francesco Marchesi, Giulia Piaggio, Diana Giannarelli, Elena Papa, Paolo Falcucci, Martina Pontone, Simona Di Martino, Valentina Laquintana, Antonia La Malfa, Enea Gino Di Domenico, Ornella Di Bella, Gianluca Falzone, Fabrizio Ensoli, Branka Vujovic, Aldo Morrone, Gennaro Ciliberto, Andrea Mengarelli
Summary: The study assessed the safety and immunogenicity of the BNT162b2 mRNA vaccine in hematological patients, finding lower response rates in multiple myeloma patients. It suggests that these patients should continue to maintain protective measures and monitor vaccine effectiveness post-vaccination.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2021)
Letter
Oncology
Francesco Marchesi, Fulvia Pimpinelli, Diana Giannarelli, Livia Ronchetti, Elena Papa, Paolo Falcucci, Martina Pontone, Enea Gino Di Domenico, Simona di Martino, Valentina Laquintana, Chiara Mandoj, Laura Conti, Iole Cordone, Antonia La Malfa, Caterina Viggiani, Daniela Renzi, Francesca Palombi, Atelda Romano, Francesco Pisani, Svitlana Gumenyuk, Ornella Di Bella, Branka Vujovic, Aldo Morrone, Gennaro Ciliberto, Fabrizio Ensoli, Andrea Mengarelli
Article
Biochemistry & Molecular Biology
Sara Donzelli, Francesca Spinella, Enea Gino Di Domenico, Martina Pontone, Ilaria Cavallo, Giulia Orlandi, Stefania Iannazzo, Giulio Maria Ricciuto, ISG Virology Covid Team, Raul Pellini, Paola Muti, Sabrina Strano, Gennaro Ciliberto, Fabrizio Ensoli, Stefano Zapperi, Caterina A. M. La Porta, Giovanni Blandino, Aldo Morrone, Fulvia Pimpinelli
Summary: The presence of undiscovered SARS-CoV-2 variants in Italy is described in this study. Deep sequencing analysis of respiratory samples revealed the D614G and S939F mutations. Unlike D614G, the S939F mutation has a slight but significant impact on immune response.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Immunology
Flaminia Campo, Aldo Venuti, Fulvia Pimpinelli, Elva Abril, Giovanni Blandino, Laura Conti, Armando De Virgilio, Federico De Marco, Vincenzo Di Noia, Enea Gino Di Domenico, Simona Di Martino, Fabrizio Ensoli, Diana Giannarelli, Chiara Mandoj, Francesco Mazzola, Silvia Moretto, Gerardo Petruzzi, Fabrizio Petrone, Barbara Pichi, Martina Pontone, Antonello Vidiri, Branka Vujovic, Giulia Piaggio, Eleonora Sperandio, Valentina Rosati, Francesco Cognetti, Aldo Morrone, Gennaro Ciliberto, Raul Pellini
Summary: The study indicates that the BNT162b2 vaccine shows antibody persistence at 6 months after the first dose, with a certain decline. Age, gender, and previous COVID-19 infection have an impact on immune response levels.
Article
Microbiology
Francesca Sivori, Ilaria Cavallo, Daniela Kovacs, Maria Guembe, Isabella Sperduti, Mauro Truglio, Martina Pasqua, Grazia Prignano, Arianna Mastrofrancesco, Luigi Toma, Fulvia Pimpinelli, Aldo Morrone, Fabrizio Ensoli, Enea Gino Di Domenico
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) has become the leading cause of skin and soft tissue infections (SSTIs) and biofilm formation contributes to increased antibiotic tolerance. This study demonstrates that dalbavancin is the most effective antibiotic against MRSA biofilms and extracellular DNA affects its activity. The findings provide new insights for developing targeted therapeutic strategies against MRSA.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Enea Gino Di Domenico, Alessandra Oliva, Maria Guembe
Summary: Biofilm-related infections pose a significant challenge to healthcare professionals due to the ability of microorganisms to form biofilm, which leads to treatment resistance and reduced susceptibility to antibiotics. Current microbiological diagnosis and antibiotic susceptibility testing methods are often inadequate for accurately assessing biofilm-related infections. Timely identification of these infections is crucial for guiding appropriate treatment strategies.
Article
Infectious Diseases
Ilaria Cavallo, Ilaria Lesnoni La Parola, Francesca Sivori, Luigi Toma, Tatiana Koudriavtseva, Isabella Sperduti, Daniela Kovacs, Giovanna D'Agosto, Elisabetta Trento, Norma Cameli, Anna Mussi, Alessandra Latini, Aldo Morrone, Fulvia Pimpinelli, Enea Gino Di Domenico
Summary: This study analyzed clinical data and laboratory parameters of patients with infected and noninfected chronic venous leg ulcers (VU). The results showed that infected ulcers had increased size and depth and higher levels of inflammatory cytokines. Hyperhomocysteinemia was more common in infected ulcers, and Gram-negative bacteria and strong biofilm producers were predominant in the infection.
Article
Multidisciplinary Sciences
Ilaria Cavallo, Francesca Sivori, Mauro Truglio, Flavio De Maio, Federica Lucantoni, Giorgia Cardinali, Martina Pontone, Thierry Bernardi, Maurizio Sanguinetti, Bruno Capitanio, Antonio Cristaudo, Fiorentina Ascenzioni, Aldo Morrone, Fulvia Pimpinelli, Enea Gino Di Domenico
Summary: This study explores the microbiome at different skin sites in adolescent acne and the role of biofilm production in promoting the growth and persistence of Cutibacterium acnes isolates. The findings show that there is lower microbiota diversity in inflammatory lesions compared to non-inflammatory lesions and healthy subjects, and there is an overabundance of C. acnes in inflammatory lesions. Additionally, specific Cutibacterium acnes phylotypes were found to be more represented in the skin of acne patients and were associated with early adhesion, biomass production, and increased antibiotic tolerance.
SCIENTIFIC REPORTS
(2022)
Review
Infectious Diseases
Alessandra Oliva, Sergio Carbonara, Vito Cianci, Massimo Crapis, Enea Gino Di Domenico, Marco Falcone, Gioacchino Galardo, Emanuele Durante-Mangoni, Mario Venditti
Summary: Acute Bacterial Skin and Skin Structure Infections (ABSSSIs) commonly result in Emergency Department (ED) access and hospital admissions, causing a significant economic burden. Long-acting lipoglycopeptides (LALs) allow for outpatient management of ABSSSI patients who require parenteral therapy but do not need hospitalization. This article discusses the microbiological activity, efficacy, and safety of dalbavancin, critical steps for ABSSSI management in the ED, and the potential advantage of direct/early discharge with dalbavancin.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2023)
Article
Chemistry, Physical
Laura Chronopoulou, Roya Binaymotlagh, Sara Cerra, Farid Hajareh Haghighi, Enea Gino Di Domenico, Francesca Sivori, Ilaria Fratoddi, Silvano Mignardi, Cleofe Palocci
Summary: In this study, a new composite material was developed by impregnating self-assembling Fmoc-Phe3 peptide hydrogels with in situ prepared silver nanoparticles (AgNPs). Different methodologies for preparing AgNPs were compared. The obtained composites were characterized for their hydrodynamic, structural, and morphological properties, and the antibacterial properties against S. aureus were tested. The results showed that the hydrogel composites containing AgNPs could potentially be used as biomaterials for treating S. aureus-related infections.
Review
Microbiology
Ilaria Cavallo, Alessandra Oliva, Rebecca Pages, Francesca Sivori, Mauro Truglio, Giorgia Fabrizio, Martina Pasqua, Fulvia Pimpinelli, Enea Gino Di Domenico
Summary: Acinetobacter baumannii is a major pathogen in vulnerable and critically ill patients, causing various infections with high mortality rates. Carbapenems have been the first choice for treatment, but widespread prevalence of carbapenem-resistant A. baumannii has led to the use of colistin as the main therapeutic option. However, high clinical failure rates have been reported for colistin monotherapy. Additionally, A. baumannii forms biofilms on medical devices, posing a significant treatment challenge.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Infectious Diseases
Alessandra Oliva, Francesco Giuseppe De Rosa, Malgorzata Mikulska, Federico Pea, Maurizio Sanguinetti, Carlo Tascini, Mario Venditti
Summary: Invasive Candida Infections (ICIs) have experienced significant changes in epidemiology, pathophysiology, and clinical manifestations in recent decades, including a shift towards non-albicans species and an increase in exogenous infections. The long-acting echinocandin rezafungin has shown potent in-vitro activity against Candida spp., including azole-resistant strains and C.auris.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2023)
Article
Polymer Science
Roya Binaymotlagh, Alessandra Del Giudice, Silvano Mignardi, Francesco Amato, Andrea Giacomo Marrani, Francesca Sivori, Ilaria Cavallo, Enea Gino Di Domenico, Cleofe Palocci, Laura Chronopoulou
Summary: This study investigated the synthesis of peptide-based hydrogel composites containing photo-generated silver nanoparticles (AgNPs) and the effects of honey on their properties. The results showed that honey increased the swelling ability and mechanical stability of the hydrogel. Additionally, the presence of honey enhanced the antibacterial activity of AgNPs in the hydrogel.
