Article
Immunology
Maria Trelis, Christian M. Sanchez-Lopez, Liz F. Sanchez-Palencia, Victor Ramirez-Toledo, Antonio Marcilla, Dolores Bernal
Summary: The protein cargo of extracellular vesicles (EVs) from different developmental stages of Fasciola hepatica, including embryonated eggs and newly-excysted juvenile (NEJ) flukes, have been analyzed. The analysis revealed qualitative differences in composition, reflecting the intense activity of NEJs at this early stage, with several proteins involved in membrane traffic and cell physiology.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Keigo Nakamura, Kazuya Kusama, Masatoshi Hori, Kazuhiko Imakawa
Summary: The study revealed that bta-miR-26b derived from intrauterine EVs contributes to the down-regulation of the maternal immune system, facilitating conceptus implantation to the uterine endometrium. Furthermore, intrauterine EVs extracted from P20 UFs could regulate neutrophils to modulate endometrial immune and inflammatory responses for implanting conceptuses.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Veterinary Sciences
Kazuhiko Imakawa, Yuta Matsuno, Hiroshi Fujiwara
Summary: This passage introduces the sine qua non of new life as fertilization and highlights the failure rates of fertilized eggs/blastocysts in cattle and human assisted reproductive procedures. It discusses the physiological events involved in the implantation of blastocysts to the maternal endometrium and the importance of EVs with miRNAs and lncRNAs in establishing the proper uterine environment.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Bin Sheng, Niansheng Lai, Tao Tao, Xiangxin Chen, Sen Gao, Qi Zhu, Wei Li, Qingrong Zhang, Chunhua Hang
Summary: This study characterized plasma extracellular vesicles in patients with aSAH using microRNAs (miRNAs) as diagnostic tools. Next-generation sequencing identified four differentially expressed miRNAs, which were validated in a large cohort. The results showed that miR-369-3p, miR-410-3p, miR-193b-3p, and miR-486-3p could predict the neurological outcomes of aSAH patients and have potential clinical utility as prognostic biomarkers.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Environmental Sciences
Ying Qiao, Xiaowan Ma, Lixing Huang, Shengping Zhong, Yongze Xing, Xuyang Chen
Summary: Molting is a crucial process in crustaceans that affects their growth, development, and reproduction. This study investigated the role of extracellular vesicles (EVs) and their microRNAs (miRNAs) in the molting process of the mud crab Scylla paramamosain. The results showed that EVs miRNAs mainly regulate immune-related genes and mediate the ecdysone signaling and insulin signaling pathways, influencing the molting process. This study provides valuable insights into the mechanisms underlying crustacean molting.
FRONTIERS IN MARINE SCIENCE
(2022)
Review
Cell Biology
Yu-Run Liu, Ya-Qi Cheng, Shou-Bi Wang, Ya-Ru Su, Ying Liu, Chao-Yang Li, Lin Jin, Qi Wan, Xuan Sang, Zhi-Chong Wang
Summary: Cancer is now considered an age-related disease, and reversing cell senescence can prevent and treat cancer fundamentally. Current anticancer treatments induce both cancer cells apoptosis and normal cells senescence, promoting a vicious circle. In the tumor microenvironment, EVs of senescent cells can change the expression profile of cancer cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Helena Sork, Mariana Conceicao, Giulia Corso, Joel Nordin, Yi Xin Fiona Lee, Kaarel Krjutskov, Jakub Orzechowski Westholm, Pieter Vader, Marie Pauwels, Roosmarijn E. Vandenbroucke, Matthew J. A. Wood, Samir EL Andaloussi, Imre Mager
Summary: The study found that most miRNAs were secreted in the non-EV fraction, with over 96% of total miRNA found in non-EVs. Despite containing only a fraction of the secreted miRNAs, EVs were stable at 37 degrees Celsius in a serum-containing environment.
Article
Virology
Steven Kopcho, Marina McDew-White, Wasifa Naushad, Mahesh Mohan, Chioma M. M. Okeoma
Summary: This study investigated the abundance and compartmentalization of blood plasma extracellular miRNA (exmiRNA) in SIV-infected rhesus macaques (RMs) and the impact of combination antiretroviral therapy (cART) and phytocannabinoid delta-9-tetrahydrocannabinol (THC) on exmiRNA. The results showed that cART and THC treatment had distinct effects on the enrichment and compartmentalization pattern of exmiRNAs. Longitudinal analysis also revealed the long-term effects of SIV infection and treatment on exmiRNAs. These findings have important implications for understanding the effects of SIV infection and treatment and for identifying disease biomarkers.
Article
Biochemistry & Molecular Biology
Carina Levin, Ariel Koren, Annie Rebibo-Sabbah, Maya Levin, Na'ama Koifman, Benjamin Brenner, Anat Aharon
Summary: In beta thalassemia major, higher levels of small EVs (exosomes) with altered miRNA expression were found compared to controls. Beta T-EVs induced cell apoptosis and decreased cell survival while promoting BM-MSC proliferation, particularly through the upregulation of miR-144-3p. These mechanisms may contribute to organ dysfunction and complications in beta thalassemia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Forough Alemi, Fatemeh Sadeghsoltani, Khashayar Fattah, Parisa Hassanpour, Faezeh Malakoti, Sina Kardeh, Melika Izadpanah, Debora Aparecida Pires de Campos Zuccari, Bahman Yousefi, Maryam Majidinia
Summary: Noncoding RNAs play important roles in key cell biological and pathological events, and their altered expression is directly and indirectly linked to cancer progression. Numerous studies have highlighted the significant role of ncRNAs in cancer prevention and therapy, making them an interesting subject for cancer treatment. However, there are limitations in the application of ncRNAs in cancer treatment, such as delivery, uptake, and short half-life. Exosomes are introduced as promising options for delivering ncRNAs to target cells. This review briefly discusses the application and barriers of ncRNAs, with a focus on exosome-based ncRNA delivery, advantages, and the latest achievements in drugging ncRNAs with exosomes.
CHEMICAL BIOLOGY & DRUG DESIGN
(2023)
Article
Immunology
Daniel Alfandari, Hila Ben Ami Pilo, Paula Abou Karam, Osnat Dagan, Carine Joubran, Ron Rotkopf, Neta Regev-Rudzki, Ziv Porat
Summary: Extracellular vesicles (EVs) play a crucial role in cell-cell communication processes and are especially important for pathogens like malaria parasites. Monitoring the uptake of malaria-derived EVs by host cells using imaging flow cytometry has been demonstrated. By staining the RNA cargo and monitoring its distribution, the kinetics of cargo delivery and distribution parameters can be evaluated.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Genetics & Heredity
Qiuyan Weng, Yao Wang, Yaoyao Xie, Xiuchong Yu, Shuangshuang Zhang, Jiaxin Ge, Zhe Li, Guoliang Ye, Junming Guo
Summary: This review summarizes the biogenesis and classification of tRFs, discusses the biological functions of EV-associated tRFs, and explores their roles as potential biomarkers in human diseases.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Review
Biochemistry & Molecular Biology
Julia Makarova, Diana Maltseva, Alexander Tonevitsky
Summary: This mini review discusses the progress of extracellular RNA (exRNA) research, with a focus on exRNA carriers. The key methodological challenges are analyzed, and recommendations on how to overcome them are provided.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Tine Tesovnik, Barbara Jenko Bizjan, Robert Sket, Marusa Debeljak, Tadej Battelino, Jernej Kovac
Summary: EV cargoes, including DNA and RNA nucleotide sequences, play a crucial role in regulating cellular processes and eliciting responses. The diversity and biological function of EV nucleotide sequences vary among EV subgroups, posing challenges in their analysis. Advanced techniques like next-generation sequencing offer sensitive and accurate detection of EV biomarkers, leading to potential therapeutic applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Lily Tran, Hayley Toet, Travis Beddoe
Summary: Fasciola hepatica, commonly known as liver flukes, is a significant parasitic disease affecting humans and livestock worldwide. Drug resistance has become a global issue, necessitating the implementation of an integrated parasite management program. A rapid DNA test using loop-mediated isothermal amplification was developed for the detection of F. hepatica from faecal and water samples, enabling real-time decision making to control parasite prevalence.
Article
Biochemical Research Methods
Eduardo de la Torre-Escudero, Ricardo Perez-Sanchez, Raul Manzano-Roman, Ana Oleaga
JOURNAL OF PROTEOMICS
(2015)
Article
Biochemical Research Methods
Krystyna Cwiklinski, Eduardo de la Torre-Escudero, Maria Trelis, Dolores Bernal, Philippe J. Dufresne, Gerard P. Brennan, Sandra O'Neill, Jose Tort, Steve Paterson, Antonio Marcilla, John P. Dalton, Mark W. Robinson
MOLECULAR & CELLULAR PROTEOMICS
(2015)
Review
Parasitology
Eduardo de la Torre-Escudero, Adam P. S. Bennett, Alexzandra Clarke, Gerard P. Brennan, Mark W. Robinson
TRENDS IN PARASITOLOGY
(2016)
Review
Biochemistry & Molecular Biology
Eduardo de la Torre-Escudero, Ricardo Perez-Sanchez, Raul Manzano-Roman, Ana Oleaga
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2017)
Article
Biochemical Research Methods
E. De la Torre Escudero, R. Manzano-Roman, L. Valero, A. Oleaga, R. Perez-Sanchez, A. Hernandez-Gonzalez, M. Siles-Lucas
JOURNAL OF PROTEOMICS
(2011)
Article
Biochemical Research Methods
Eduardo de la Torre-Escudero, Luz Valero, Ricardo Perez-Sanchez, Raul Manzano-Roman, Ana Oleaga
JOURNAL OF PROTEOMICS
(2012)
Article
Biochemical Research Methods
Eduardo de la Torre-Escudero, Ricardo Perez-Sanchez, Raul Manzano-Roman, Ana Oleaga
JOURNAL OF PROTEOMICS
(2013)
Article
Biochemical Research Methods
Eduardo y de la Torre-Escudero, Ricardo Perez-Sanchez, Raul Manzano-Roman, Ana Oleaga
JOURNAL OF PROTEOMICS
(2014)
Article
Parasitology
Eduardo de la Torre-Escudero, Raul Manzano-Roman, Ricardo Perez-Sanchez, Inmaculada Barrera, Mar Siles-Lucas, Ana Oleaga
VETERINARY PARASITOLOGY
(2012)
Article
Parasitology
Eduardo de la Torre-Escudero, Raul Manzano-Roman, Mar Siles-Lucas, Ricardo Perez-Sanchez, J. Carlos Moyano, Inmaculada Barrera, Ana Oleaga
VETERINARY PARASITOLOGY
(2012)
Article
Infectious Diseases
Eduardo de la Torre-Escudero, Jared Q. Gerlach, Adam P. S. Bennett, Krystyna Cwiklinski, Heather L. Jewhurst, Kathryn M. Huson, Lokesh Joshi, Michelle Kilcoyne, Sandra O'Neill, John P. Dalton, Mark W. Robinson
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Parasitology
Adam P. S. Bennett, Eduardo de la Torre-Escudero, Nicola A. M. Oliver, Kathryn M. Huson, Mark W. Robinson
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2020)
Article
Parasitology
Adam P. S. Bennett, Eduardo de la Torre-Escudero, Mark W. Robinson
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Adam P. S. Bennett, Eduardo de la Torre-Escudero, Susan S. E. Dermott, Lawrence T. Threadgold, Robert E. B. Hanna, Mark W. Robinson
Summary: This study used laser microdissection to analyze the proteome of the gastrodermal cells in the liver fluke. The results showed that these cells are the main source of secreted proteins and may release extracellular vesicles. The study also highlighted the importance of the gastrodermal cells in nutrient acquisition and immunomodulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)