期刊
INTERNATIONAL JOURNAL OF CANCER
卷 139, 期 4, 页码 824-835出版社
WILEY
DOI: 10.1002/ijc.30131
关键词
angiogenesis; galectin-1; antibody; imaging; therapy; sprouting
类别
资金
- Center for Translational Molecular Medicine Mammary Carcinoma Molecular Imaging for Diagnosis and Therapeutics (CTMM-MAMMOTH) [2010249, 03O-201]
- Dutch Cancer Foundation [KWF: VU2009-4358, VU2012-5715]
- European Union [PIEF-GA-2013-626797]
Galectins are carbohydrate binding proteins that function in many key cellular processes. We have previously demonstrated that galectins are essential for tumor angiogenesis and their expression is associated with disease progression. Targeting galectins is therefore a potential anti-angiogenic and anti-cancer strategy. Here, we used a rational approach to generate antibodies against a specific member of this conserved protein family, i.e. galectin-1. We characterized two novel mouse monoclonal antibodies that specifically react with galectin-1 in human, mouse and chicken. We demonstrate that these antibodies are excellent tools to study galectin-1 expression and function in a broad array of biological systems. In a potential diagnostic application, radiolabeled antibodies showed specific targeting of galectin-1 positive tumors. In a therapeutic setting, the antibodies inhibited sprouting angiogenesis in vitro and in vivo, underscoring the key function of galectin-1 in this process.
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