期刊
INTERNATIONAL JOURNAL OF CANCER
卷 139, 期 10, 页码 2261-2269出版社
WILEY
DOI: 10.1002/ijc.30289
关键词
malignant pleural effusion (MPE); CD4+T cells; CD8+T cells; TGF-beta; tumor-associated macrophages (TAMs); impaired T cell function
类别
资金
- National Natural Science Foundation of China [81171986]
- Ministry of Public Health [201501004]
- Basic and Advanced Technology Research Foundation from Science and Technology Department of Henan Province [112300410153, 122300410155]
- Funds for Creative Research Team of Henan Province, Creative Research Team of Higher Education of Henan Province
Malignant pleural effusion (MPE) is an indication of advanced cancer. Immune dysfunction often occurs in MPE. We aimed to identify the reason for impaired T cell activity in MPE from lung cancer patients and to provide clues toward potential immune therapies for MPE. The surface inhibitory molecules and cytotoxic activity of T cells in MPE and peripheral blood (PB) were analyzed using flow cytometry. Levels of inflammatory cytokines in MPE and PB were tested using ELISA. TGF-beta expression in tumor-associated macrophages (TAMs) was also analyzed. The effect of TAMs on T cells was verified in vitro. Lastly, changes in T cells were evaluated following treatment with anti-TGF-beta antibody. We found that expression levels of Tim-3, PD-1 and CTLA-4 in T cells from MPE were upregulated compared with those from PB, but levels of IFN-gamma and Granzyme B were down-regulated (p < 0.05). The amount of TGF-beta was significantly higher in MPE than in PB (p < 0.05). TGF-beta was mainly produced by TAMs in MPE. When T cells were co-cultured with TAMs, expression levels of Tim-3, PD-1 and CTLA-4 were significantly higher than controls, whereas levels of IFN-gamma and Granzyme B were significantly decreased, in a dose-dependent manner (p < 0.05). In vitro treatment with anti-TGF-beta antibody restored the impaired T cell cytotoxic activity in MPE. Our results indicate that macrophage-derived TGF-beta plays an important role in impaired T cell cytotoxicity. It will therefore be valuable to develop therapeutic strategies against TGF-beta pathway for MPE therapy of lung cancer.
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