Article
Engineering, Environmental
Lifan Zeng, Zece Zhu, Ruiqi Mo, Wei Li, Weilin Xu, Di Tian
Summary: Clusteroluminescence from non-conjugated organic molecules has been extensively studied. This study demonstrates the use of chitosan as a renewable polymer with persistent clusteroluminescence. The phosphorescence intensity and lifetime were enhanced and regulated by the external heavy-atom effect. The doping concentration of bromide salts in chitosan allowed for gradual adjustment of the phosphorescence lifetime from 49 to 647 ms. This strategy can be applied to other phosphorescent materials and enables label-free luminescence imaging of chitin-containing organisms.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemical Research Methods
Kari Kopra, Nazia Hassan, Emmiliisa Vuorinen, Salla Valtonen, Randa Mahran, Huda Habib, Pinja Jalkanen, Petri Susi, Vesa Hytonen, Minna Hankaniemi, Seppo Yla-Herttuala, Laura Kakkola, Markus Peurla, Harri Harma
Summary: Viruses play a major role in modern society, but current methods for counting viral particles are cumbersome and expensive. To address this issue, researchers have developed a simple and cost-effective time-resolved luminescence-based method that allows for rapid measurement of virus particle count.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Optics
Pavel Morozov, Maria Lukina, Marina Shirmanova, Alexander Divochiy, Varvara Dudenkova, Gregory N. Gol'tsman, Wolfgang Becker, Vladislav Shcheslavskiy
Summary: This letter introduces a novel optical configuration for direct time-resolved measurements of luminescence from singlet oxygen in solutions and cultured cells, using a superconducting single-photon detector. The system is verified by measuring phosphorescence signals from singlet oxygen generated by commonly used photosensitizers in photodynamic therapy.
Article
Chemistry, Analytical
Qisheng Deng, Zehua Lan, Li Xu, Zece Zhu, Xuewen Shu
Summary: Time-resolved luminescence imaging is typically conducted on Lab instruments with high-speed excitation sources and detectors, which are often bulky and costly. By utilizing a FPGA-based digital circuit and a smartphone camera, microsecond-resolved luminescence lifetime imaging was achieved for the first time. This miniaturized apparatus shows promise for time-resolved luminescence detection and imaging applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Multidisciplinary Sciences
Tongcheng Qian, Tiffany M. Heaster, Angela R. Houghtaling, Kexin Sun, Kayvan Samimi, Melissa C. Skala
Summary: The study presents a non-invasive label-free live cell imaging platform based on autofluorescence imaging to predict efficiency of hPSC differentiation into cardiomyocytes. This method provides high accuracy to separate differentiating cells within 1 day, avoiding variability in cell manufacturing.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Evgeny A. Shirshin, Marina V. Shirmanova, Alexey V. Gayer, Maria M. Lukina, Elena E. Nikonova, Boris P. Yakimov, Gleb S. Budylin, Varvara V. Dudenkova, Nadezhda I. Ignatova, Dmitry V. Komarov, Vladislav V. Yakovlev, Wolfgang Becker, Elena V. Zagaynova, Vladislav I. Shcheslavskiy, Marlan O. Scully
Summary: The heterogeneity of cells determines their response to external factors, and the metabolic heterogeneity of tumor cells is of clinical relevance. Fluorescence lifetime imaging (FLIM) is a non-invasive technique that can sense and quantify cellular metabolism by measuring the fluorescence decay parameters of endogenous fluorophores. This study demonstrates the unique capability of FLIM in accurately discriminating metabolic heterogeneity using a non-parametric analysis approach, which has been validated in cultured cancer cells treated with chemotherapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Maria Lukina, Konstantin Yashin, Elena E. Kiseleva, Anna Alekseeva, Varvara Dudenkova, Elena V. Zagaynova, Evgenia Bederina, Igor Medyanic, Wolfgang Becker, Deependra Mishra, Mikhail Berezin, Vladislav I. Shcheslavskiy, Marina Shirmanova
Summary: The development of a rapid and sensitive assay for intraoperative diagnostics of glioma, focusing on fluorescence lifetime imaging (FLIM) to identify imaging markers for differentiation between tumors and healthy tissues, shows a high potential for diagnostics of glioma and evaluation of surgical margins.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, Research & Experimental
Liping Chen, Guihui Qin, Yuhong Liu, Moxin Li, Yue Li, Lun-Zhang Guo, Lidong Du, Weiming Zheng, Pei-Chun Wu, Yueh-Hsun Chuang, Xiaoyan Wang, Tzung-Dau Wang, Ja-An Annie Ho, Tzu-Ming Liu
Summary: The study identifies distinguishable features of prediabetic adipose tissues through metabolic imaging, providing a potential diagnostic method for prediabetes. In prediabetic adipose tissues, only adipocytes exhibit a low redox ratio of metabolic fluorescence and high free NAD(P)H fraction, which could be a risk indicator of prediabetic insulin resistance.
Article
Optics
Moosung Lee, Kyoohyun Kim, Jeonghun Oh, YongKeun Park
Summary: This study demonstrates isotropic microtomography of free-floating samples by optically rotating a sample, using 3D structured light traps to measure side scattering information for achieving isotropic resolution. The method yields a resolution of 230 nm and captures structural details inaccessible using conventional tomographic microscopy.
LIGHT-SCIENCE & APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Jungwoo Kim, Soo Bin Ahn, Subin Hong, Kwang-sun Kim, Esther Ha-eun Ko, I. Jeong Jo, JuOae Chang, Meehyein Kim, Wonsik Lee, Haemi Lee
Summary: Nanoscopic investigation of bacterial cells is crucial for understanding their physiological status and cellular functions. A new technique called intracellular dynamics-resolved Rayleigh scattering (IDRS) has been developed, which can visualize spatiotemporal cytoplasmic transitions in unlabeled bacteria and characterize their real-time physiological status. IDRS signals can differentiate metabolic statuses and antibiotic resistance in unlabeled bacteria, providing insights for controlling bacterial metabolism and efficacy at the single-cell level.
Article
Nanoscience & Nanotechnology
Wenxuan Liang, Defu Chen, Honghua Guan, Hyeon-Cheol Park, Kaiyan Li, Ang Li, Ming-Jun Li, Israel Gannot, Xingde Li
Summary: This article introduces a newly developed two-photon fluorescence lifetime imaging endomicroscope that can extract NADH lifetime parameters of living tissues and organs, track changes in cellular metabolic status and tumor models, and promises real-time dynamic imaging in clinical diagnosis and therapeutic applications.
Article
Chemistry, Multidisciplinary
Bolong Zhang, Kieran D. Richards, Beatrice E. Jones, Abigail R. Collins, Rosie Sanders, Sarah R. Needham, Pu Qian, Amoghavarsha Mahadevegowda, Caterina Ducati, Stanley W. Botchway, Rachel C. Evans
Summary: This study demonstrates the first example of triplet-triplet annihilation upconversion (TTA-UC) based lifetime imaging microscopy. Ultra-small nanoparticle (NP) probes were used to overcome background autofluorescence and achieve excellent image contrast and oxygen mapping in living cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Multidisciplinary
Xinyan Zhu, Xiaohan Wang, Hongxin Zhang, Fan Zhang
Summary: Luminescence lifetime imaging has advantages over traditional optical imaging in terms of precise quantification detection, and the long luminescence lifetime of lanthanide-doped nanoparticles (LnNPs) has attracted great attention. LnNPs with long luminescence lifetime not only make it easier to filter out background signals during imaging, but also provide a wide range of tunable lifetime. This article introduces the luminescence mechanisms of LnNPs, strategies for luminescence lifetime modulation, data acquisition methods, and cutting-edge applications in lifetime imaging systems, as well as prospects for improving technologies and extending applications of luminescence lifetime imaging.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Optics
Natan T. Shaked, Stephen A. Boppart, Lihong V. Wang, Juergen Popp
Summary: This Review provides a comprehensive overview and comparison of different label-free imaging modalities, aiming to facilitate collaboration, technological advancements, and biophysical discoveries, as well as new avenues in clinical detection, diagnosis, and monitoring of diseases.
Review
Engineering, Biomedical
Irene Georgakoudi, Kyle P. Quinn
Summary: In the past fifty years, the autofluorescence of NADH and FAD has been studied in various cell types and diseases. With the advancement of nonlinear optical microscopy in biomedical research, NADH and FAD imaging provides a noninvasive method to monitor cell and tissue status and understand dynamic changes in metabolism. Different tools and methods have been developed to measure the properties of NADH and FAD autofluorescence, but further work is needed to fully understand metabolism. This article discusses the current understanding of our optical sensitivity to metabolic pathways and the challenges in the field.
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING
(2023)