期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 93, 期 -, 页码 879-888出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2016.09.055
关键词
Baphicacanthis Cusiae Rhizoma et Radix; Polysaccharide; Anti-inflammatory activity
资金
- Guangdong Province for the Construction of High Level University [2050205]
- Ministry of Science and Technology of China [2014DFH30010]
The purpose of this study was to investigate the extraction, characterization and bioactivities of purified water-soluble polysaccharides (BCP) from Baphicacanthis Cusiae Rhizoma et Radix. Based on the response surface methodology, the optimal extraction parameters were obtained as follows: extraction temperature of 60.0 C, extraction time of 35.0 min, and ratio of water to raw material of 24.5 mlig. Then, BCP was separated and purified by chromatography of DEAE-52 and Sephadex G-100, and obtained two purified fractions, named as BCP-1 and BCP-2. Their molecular weights were respectively 11.6 and 26.7 KDa with mainly composed of glucose, arabinose and galactose. BCP-2 had higher contents of sulfuric radical and uronic acid than BCP-1. Finally, their antioxidant and anti-inflammatory activities were evaluated. Both of BCP-1 and BCP-2 exhibited strong antioxidant activity in vitro, and the antioxidant of BCP-2 was better. Besides, they showed ideal anti-inflammatory activity in vitro and in vivo. (C) 2016 Elsevier B.V. All rights reserved.
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