Article
Multidisciplinary Sciences
Albert Cairo, Anna Vargova, Neha Shukla, Claudio Capitao, Pavlina Mikulkova, Sona Valuchova, Jana Pecinkova, Petra Bulankova, Karel Riha
Summary: In Arabidopsis, the transition from meiosis to mitosis is driven by translation inhibition involving processing bodies. The meiosis-specific protein TDM1 is incorporated into processing bodies through interaction with SMG7, sequestering the main translation initiation complex and inhibiting translation. Chemical inhibition of translation can overcome the failure to terminate meiosis in tdm1 mutants. TDM1-containing processing bodies down-regulate meiotic transcripts to facilitate the transition of cell fates.
Article
Multidisciplinary Sciences
Stuart D. Desjardins, James Simmonds, Inna Guterman, Kostya Kanyuka, Amanda J. Burridge, Andrew J. Tock, Eugenio Sanchez-Moran, F. Chris H. Franklin, Ian R. Henderson, Keith J. Edwards, Cristobal Uauy, James D. Higgins
Summary: FANCM suppresses crossovers in plants and maintains obligatory crossovers in wheat, promoting generation of novel allelic combinations. It plays a role in first-end capture and non-crossover formation. Additionally, fancm could be an effective tool for accelerating breeding in wheat.
NATURE COMMUNICATIONS
(2022)
Article
Developmental Biology
Huan Yin, Suye Suye, Zhixian Zhou, Haiyi Cai, Chun Fu
Summary: Defects in Fance lead to a progressive reduction of oocytes and disrupt the progression of meiotic prophase I. The potential mechanisms involve DNA damage repair, meiotic crossover, and pluripotency of oocytes.
Article
Multidisciplinary Sciences
Irene M. Haefliger, Mirjam Spengeler, Franz R. Seefried, Cord Droegemueller
Summary: By utilizing extensive genotyping and whole-genome sequencing data, we have identified multiple haplotype regions in Holstein cattle and discovered novel deleterious protein-changing variants with frequencies ranging from 1% to 5%.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Bang-Bang Li, Shuai-Bing Zhang, Yang-Yong Lv, Shan Wei, Yuan-Sen Hu
Summary: In this study, the impact and regulatory mechanisms of protein carbonylation on wheat seed vigor during the aging process were investigated. The results showed that protein carbonylation led to a decrease in seed vigor and was related to the activity levels of antioxidant enzymes and non-enzyme antioxidants. In addition, the content of protein bodies decreased during the aging process.
Article
Biochemistry & Molecular Biology
Takatoshi Sekiguchi, Keisuke Yoshida, Ken-Ichi Wakabayashi, Toru Hisabori
Summary: This study demonstrates that the redox regulation of CFoCF1 in ATP synthesis under light conditions is influenced by the oxidation of CF1-γ subunit and physiological oxidants in the chloroplast. The generation status of Delta mu H+ controls the redox regulation of CFoCF1 to prevent energetic disadvantages in plants.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Ecology
Gust Bilcke, Koen Van den Berge, Sam De Decker, Eli Bonneure, Nicole Poulsen, Petra Bulankova, Cristina Maria Osuna-Cruz, Jack Dickenson, Koen Sabbe, Georg Pohnert, Klaas Vandepoele, Sven Mangelinckx, Lieven Clement, Lieven De Veylder, Wim Vyverman
Summary: The study integrates transcriptomic data and reveals significant changes in gene expression during sexual reproduction in diatoms in response to SIP, inhibiting processes like S-phase progression, cell division, mitosis, and inducing upregulation of meiotic genes. The research also uncovers the roles of reactive oxygen species, energy metabolism, and cGMP signaling in early sexual reproduction stages.
Article
Biochemistry & Molecular Biology
Mariana Romeiro Motta, Xin'Ai Zhao, Martine Pastuglia, Katia Belcram, Farshad Roodbarkelari, Maki Komaki, Hirofumi Harashima, Shinichiro Komaki, Manoj Kumar, Petra Bulankova, Maren Heese, Karel Riha, David Bouchez, Arp Schnittger
Summary: Flowering plants contain multiple cyclin families, with B1-type cyclins playing a crucial role in cell cycle control during Arabidopsis development. Mutant analysis revealed complex overlapping requirements of B1-type cyclins, with CYCB1;2 being central. The double mutant cycb1;1 cycb1;2, although compromised in growth, provides a unique opportunity to study the function of B1-type cyclins at the organismic level.
Article
Biology
Vivek Kumar Raxwal, Somya Singh, Manu Agarwal, Karel Riha
Summary: The study revealed that new genes in rice have relatively low expression levels, possibly controlled by distal enhancers and chromatin conformation at their TSS; the chromatin in TSS regions may be remodeled in response to abiotic stress, indicating conditional expression of young genes. Furthermore, transcripts of young genes in Arabidopsis are prone to nonsense-mediated RNA decay, restricting their expression.
Article
Biochemistry & Molecular Biology
Sheng Zuo, Ramakrishna Yadala, Fen Yang, Paul Talbert, Joerg Fuchs, Veit Schubert, Ulkar Ahmadli, Twan Rutten, Ales Pecinka, Martin A. Lysak, Inna Lermontova
Summary: The evolutionary history of the KNL2 gene in plants has been reconstructed, revealing three ancient duplications and differentiation among different plant groups. The study also suggests that the KNL2 gene is involved in centromere and/or kinetochore assembly for preserving genome stability.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Multidisciplinary Sciences
Albert Cairo, Anna Vargova, Neha Shukla, Claudio Capitao, Pavlina Mikulkova, Sona Valuchova, Jana Pecinkova, Petra Bulankova, Karel Riha
Summary: In Arabidopsis, the transition from meiosis to mitosis is driven by translation inhibition involving processing bodies. The meiosis-specific protein TDM1 is incorporated into processing bodies through interaction with SMG7, sequestering the main translation initiation complex and inhibiting translation. Chemical inhibition of translation can overcome the failure to terminate meiosis in tdm1 mutants. TDM1-containing processing bodies down-regulate meiotic transcripts to facilitate the transition of cell fates.
Article
Plant Sciences
Michael Moebes, Heike Kuhlmann, Dmitri Demidov, Inna Lermontova
Summary: This study optimized the transcriptional RT-qPCR analysis of low-expression epigenetic genes in rapeseed, using KNL2 as an example. By combining various factors, the sensitivity of RT-qPCR for detecting the expression of BnKNL2A and BnKNL2C genes was significantly increased.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Dmitri Demidov, Inna Lermontova, Michael Moebes, Andriy Kochevenko, Joerg Fuchs, Oda Weiss, Twan Rutten, Eberhard Sorge, Erika Zuljan, Ricardo Fabiano Hettwer Giehl, Martin Mascher, Saravanakumar Somasundaram, Udo Conrad, Andreas Houben
Summary: Targeted degradation of CENH3 protein can be used to generate haploids in Arabidopsis thaliana. By using a recombinant anti-GFP nanobody fused to ligase proteins, EYFP-tagged CENH3 can be recognized and degraded through the ubiquitin-proteasome pathway. This strategy achieves a haploid frequency of up to 7.6%.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Vivek K. Raxwal, Karel Riha
Summary: NMD is a conserved quality control pathway that inhibits the expression of transcripts containing premature termination codon. It plays important roles in regulating gene expression and various physiological processes in plants.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Plant Sciences
Sorin Tanasa, Neha Shukla, Albert Cairo, Ranjani S. Ganji, Pavlina Mikulkova, Sona Valuchova, Vivek K. Raxwal, Claudio Capitao, Arp Schnittger, Zbynek Zdrahal, Karel Riha
Summary: Meiosis is a specialized cell division that reduces the number of chromosomes, followed by mitotic divisions to form haploid gametophytes in angiosperm plants. In Arabidopsis, the termination of meiosis and transition to gametophytic development are regulated by TDM1 and SMG7, which inhibit translation. Mutants deficient in this mechanism show aberrant nuclear divisions due to the failure to downregulate cyclin dependent kinases during meiotic exit. A mutation in cyclin-dependent kinase D;3 (CDKD;3) was identified as a suppressor that alleviates meiotic defects in smg7 deficient plants by preventing aberrant meiotic divisions or delaying their onset. CDKD;3 acts independently of CDKA;1 and interacts with proteins involved in cytokinesis, suggesting a complex role in cell cycle regulation.
Article
Plant Sciences
Jothipriya Ramakrishnan Chandra, Manikandan Kalidass, Dmitri Demidov, Siarhei A. Dabravolski, Inna Lermontova
Summary: Centromeres are chromosomal domains that regulate the proper segregation of chromosomes during cell division by forming kinetochore protein complexes. Although their function remains conserved, their DNA sequences exhibit high variability, even among closely related species. The loading of centromeric histone H3 (CENH3) onto centromeres is epigenetically determined, and it depends on factors involved in centromere licensing, chaperones, and transcription of centromeric repeats. In this review, the variability of centromeric sequences and the regulation of their transcription through RNA Pol II and RNAi machinery are discussed, suggesting that the interaction between proteins involved in CENH3 loading, kinetochore assembly, centromeric DNA, and transcripts play a role in centromere and possibly neocentromere formation in a sequence-independent manner.