期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 86, 期 -, 页码 352-361出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2016.01.058
关键词
Coriolus versicolor; beta-glucans; Macrophages; NO; Salmonella typhimurium; Antibacterial activities
资金
- national high-tech R&D program of China (863 program) [2013AA102806, 2011AA10A215]
- national natural science foundation of China [31272541, 31272552]
- national program on key basic research project of China (973 program) [2012CB722202]
- Jilin province science and technology development program of China [20111816]
- Jilin province quality and safety of agricultural products program by world bank [2011-Y07]
The effects of beta-glucans from Coriolus versicolor (CVP), which are extracted from a well-known immune stimulator C. versicolor, have been demonstrated extensively in vitro and in vivo. However, until now, the phagocytic activity has not been elucidated. Hence, the objective of the present study was to identify the antibacterial activity of CVP or CVP-treated macrophages by an analysis of cell cytotbxicity, phagocytic activity, intracellular bacterial survival, macrophage activation, production of nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) in CVP-treated macrophages using flow cytometry, RT-PCR, a gentamicin protection assay, a Nitric oxide assay and an iNOS enzymatic activity assay. The results indicate that CVP-treated macrophages can phagocytize and kill bacteria, probably due to the production of NO and iNOS. More importantly, CVP-treated macrophages are effective at protecting mice against the challenge of Salmonella typhimurium, The results of this study suggest that the antibacterial effects of CVP are probably caused by the activation of innate immune cells, especially macrophages, because the activated macrophage produces NO, which kills bacteria. These phenomena indicate the possibility of CVP as a potential alternative for antibiotics against resistant bacteria. (C) 2016 Elsevier B.V. All rights reserved.
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