Article
Biochemistry & Molecular Biology
Fengchao Zhang, Zheng Fu, Luyao Tang, Zhelun Zhang, Feng Han, Wengong Yu
Summary: The novel exo-type alginate lyase VsAly7D isolated from brown algae showed the highest activity at 35 degrees C, with considerable activity at 20 and 30 degrees C, while exhibiting optimal activity under alkaline conditions. VsAly7D demonstrated efficient degradation of alginate, polyM, and polyG, making it a promising tool enzyme for bioethanol production from brown algae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Rui Yin, Yan-Jun Yi, Zhuo Chen, Bao-Xun Wang, Xue-Han Li, Yan-Xia Zhou
Summary: In this study, a new exo-alginate lyase named Alys1 with high activity was cloned from a marine strain, showing cold-adapted characteristics and efficient degradation of alginates at low temperatures. Alys1 can depolymerize alginates polyM and polyG, producing monosaccharides as minimal alginate oligosaccharides, and demonstrates bifunctionality with a preference for M over G. These properties make Alys1 a valuable candidate for both basic research and industrial applications.
Article
Chemistry, Medicinal
Huiqin Huang, Shuang Li, Shixiang Bao, Kunlian Mo, Dongmei Sun, Yonghua Hu
Summary: Alg2951 is a PL7 family alginate lyase with exo/endo-type activity, showing maximum activity at 25 degrees C and pH 8.0, and a special preference for poly-guluronic acid (polyG). It can catalyze the hydrolysis of sodium alginate to produce monosaccharides and trisaccharides, displaying good antioxidant activity.
Article
Chemistry, Medicinal
Li Sha, Minghai Huang, Xiaonan Huang, Yongtong Huang, Ensi Shao, Xiong Guan, Zhipeng Huang
Summary: A highly active novel alginate lyase was obtained from marine bacteria. The enzyme showed preference for polyM as substrate, and its activity was enhanced by K+ and inhibited by Fe2+ and Cu2+. It has promising industrial application prospects.
Article
Chemistry, Medicinal
Zhao Xue, Xiao-Meng Sun, Cui Chen, Xi-Ying Zhang, Xiu-Lan Chen, Yu-Zhong Zhang, Shou-Jin Fan, Fei Xu
Summary: Alginate oligosaccharides (AOS) have various biological activities and important applications, and alginate lyases have unique advantages in the preparation of AOS. In this study, a strain of Pseudoalteromonas arctica M9 was isolated from Sargassum, and five putative alginate lyases were identified in its genome. Among them, AlyM2 showed the highest activity and mainly produced trisaccharides from sodium alginate, indicating its potential for trisaccharide preparation.
Article
Agriculture, Multidisciplinary
Fen Yan, Junying Chen, Ting Cai, Jinfu Zhong, Shaolong Zhang
Summary: A novel bifunctional alginate lyase, BY17PV7, was cloned and expressed in Escherichia coli BL21(DE3). BY17PV7 showed the greatest activity at 43 degrees C and pH 8.9, with a higher affinity for polyG. It exhibited a wide range of substrate specificity and good degradation effects. Furthermore, the released AOS by BY17PV7 showed strong antioxidant ability.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Review
Chemistry, Applied
Ushasree Mrudulakumari Vasudevan, Ok Kyung Lee, Eun Yeol Lee
Summary: Alginate is a commonly used biopolymer in various industries, with alginate oligosaccharides (AOS) showing promising biological activities and therapeutic potential. Enzymes and microbial processes play a role in the production of AOS from brown algae, with ongoing research exploring the structure-activity relationship of different AOS.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Wenli Zhang, Hu Ren, Xinxiu Wang, Quanyu Dai, Xiaoyong Liu, Dawei Ni, Yingying Zhu, Wei Xu, Wanmeng Mu
Summary: In this study, a thermally stable alginate lyase (ALyase) from Paenibacillus sp. YN15 (Payn ALyase) was characterized. Rational design mutagenesis was carried out to improve its thermostability, resulting in a triple-point mutant with increased optimum temperature and residual activity. This study provides a promising ALyase mutant for the industrial production of alginate oligosaccharides (AOS).
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Zhongbin Xiao, Kuikui Li, Tang Li, Fanxing Zhang, Jiayi Xue, Miao Zhao, Heng Yin
Summary: Alginate lyase VwAlg7A, a novel PL7 family exolytic alginate lyase, was successfully heterologously expressed in E. coli BL21 (DE3). VwAlg7A showed specificity towards poly-guluronate and exhibited optimal temperature and pH at 30 degrees C and 7.0, respectively. The activity of VwAlg7A could be inhibited by Ni2+, Zn2+, and NaCl, and its kinetic parameters were determined as K-m of 36.9 mg/ml and V-max of 395.6 mu M/min. ESI and HPAEC-PAD results confirmed the exolytic mode of sugar bond cleavage by VwAlg7A, and important catalytic residues were identified through molecular docking and mutagenesis experiments.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Xiao-Meng Sun, Zhao Xue, Mei-Ling Sun, Yi Zhang, Yu-Zhong Zhang, Hui-Hui Fu, Yu-Qiang Zhang, Peng Wang
Summary: In this study, a novel alginate lyase, AlyC8, with two functional catalytic domains (CD1 and CD2) was discovered. Both CD1 and CD2 showed synergistic effects in alginate degradation. The results demonstrated that CD1 plays a major role in the degradation of alginate by AlyC8, and CD1 and CD2 have different substrate specificities.
Article
Chemistry, Multidisciplinary
Marlene Vuillemin, Bo Pilgaard, Emma Kiehn, Folmer Fredslund, Ditte H. Welner, Anne S. Meyer, Finn L. Aachmann, Casper Wilkens
Summary: The research reveals that TpPL7A lacks the catalytic histidine found in PL7 alginate lyases but still utilizes a tyrosine to achieve a shared syn-beta-elimination mechanism. This mechanism may also be applicable to subfamily PL7_4 glucuronan lyases.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Mingpeng Wang, Lei Chen, Zhengyu Lou, Xueting Yuan, Guiping Pan, Xiaoyan Ren, Pengyu Wang
Summary: Alginate oligosaccharides (AOS) are low molecular weight alginate with improved water solubility and comprehensive health benefits. In this study, a novel alginate lyase called Algpt was discovered and characterized from a marine bacterium. Algpt exhibited high stability and adaptability, making it suitable for efficient preparation of AOS with different sizes and AOS-based products.
Article
Ecology
Glen D'Souza, Ali Ebrahimi, Astrid Stubbusch, Michael Daniels, Johannes Keegstra, Roman Stocker, Otto Cordero, Martin Ackermann
Summary: Bacteria break down polysaccharides through the activity of enzymes. Differences in enzymatic secretion capabilities in marine bacterial taxa can have profound effects on the pool of diffusible breakdown products and ecological dynamics. This study investigates the growth dynamics and interactions of marine Vibrionaceae strains that degrade the marine polymer alginate. The results show that strains with lower extracellular enzyme secretions aggregate more strongly than strains with higher secretions, indicating a link between enzymatic secretion capabilities and cell aggregation in marine bacteria.
Article
Biotechnology & Applied Microbiology
Xinxiu Wang, Wei Xu, Quanyu Dai, Xiaoyong Liu, Cuie Guang, Wenli Zhang, Wanmeng Mu
Summary: Currently, marine sugars have gained increased attention due to their unique physiological effects. Alginate oligosaccharides (AOS), degradation products of alginate, find applications in the fields of food, cosmetic, and medicine. A novel alginate lyase from Paenibacillus ehimensis (paeh-aly) showed potential for industrial production of AOS due to its excellent thermostability and efficiency.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shengsheng Cao, Li Li, Qian Li, Ling Jiang, Benwei Zhu, Zhong Yao
Summary: A novel alginate lyase, AlyPL17, was cloned and characterized from Pedobacter hainanensis NJ-02. It exhibited efficient catalytic activity towards polymannuronic acid, polyguluronic acid, and alginate sodium. AlyPL17 showed maximum activity at 45°C and pH 9.0, and degraded alginate through the cooperative action of two structural domains in an exolytic mode.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)