期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 93, 期 -, 页码 731-745出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2016.09.007
关键词
Lactic acid bacteria; Probiotic; Lactobacillus plantarum; Exopolysaccharides; Central composite design; Response surface methodology
资金
- Department of Biotechnology (DBT) Government of India, New Delhi [BT/IN/Indo-UK/SuBB/23/NT/2013]
- Department of Science and Technology (DST) Government of India, New Delhi [SR/FIST/DST-013/2012]
- Deanship of Scientific Research, College of Science Research Centre, King Saud University, Kingdom of Saudi Arabia
- BBSRC [BB/K020617/1] Funding Source: UKRI
In this study, 27 strains of Lactic acid bacteria (LAB) were isolated and identified from different milk sources. All the isolates were biochemically characterized and screened for their ability to produce exopolysaccharides (EPS), among which two isolates namely Lactobacillus plantarum NTMI05 (197 mg/L) and Lactobacillus plantarum NTMI20 (187 mg/L) showed higher EPS production. Both the isolates were molecular characterized and tested for their probiotic properties. The chemical composition of EPS from L. plantarum NTMI05 and NTMI20 revealed the presence of 95.45% and 92.35% carbohydrates, 14 +/- 0.1 and 11 +/- 0.15 mg/L lactic acid, 10.5 +/- 0.2 and 9 +/- 0.1 mg/mL of reducing sugar, respectively. HPLC analysis showed galactose at the retention time of 2.29.The maximum EPS yield was optimized for the media components like glucose (20 a), yeast extract (25 g/L) and ammonium sulphate (2 g/L) using Central Composite Design and Response Surface Methodology (RSM). Under optimum conditions the predicted maximum EPS production was 0.891 g/L, 0.797 g/L, while the actual experimental value was 0.956 g/L and 0.827 g/L for L. plantarum NTMI05 and NTMI20, respectively. The antioxidant capacity was also evaluated by DPPH and reducing power assay proving the potentiality of these organisms in food and dairy industries. (C) 2016 Elsevier B.V. All rights reserved.
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