High expression of tight junction protein 1 as a predictive biomarker for bladder cancer grade and staging

Tight junction proteins 1-3 (TJP1-3) are components of tight junctions that can link transmembrane proteins to the actin cytoskeleton, and their incidence directly correlates to metastasis. However, the role of the TJP family in bladder cancer has not been adequately evaluated. In this study, we evaluated the genetic changes, mRNA and protein expressions of the target genes of the TJP family in bladder cancer patients using online database and immunohistochemistry, respectively. We found that TJP1 was amplified in bladder cancer tissue and that the protein expression levels were significantly associated with age (p = 0.03), grade (p = 0.007), and stage (p = 0.011). We also examined the correlation between TJP1 and other high-frequency mutation genes using TIMER. TJP1 mRNA levels were positively correlated with TTN and RYR3 mRNA levels in bladder cancer tissue. Taken together, TJP1 expression is associated with poor clinical outcomes in patients with bladder cancer and can be a useful predictive biomarker for bladder cancer staging.

Automated summary

This study evaluated the genetic changes and expressions of the TJP family in bladder cancer and found that TJP1 was amplified in bladder cancer tissue. The protein expression levels of TJP1 were significantly associated with age, grade, and stage in patients with bladder cancer. Additionally, TJP1 expression was correlated with poor clinical outcomes in bladder cancer patients.