Review
Chemistry, Multidisciplinary
Peilin Guo, Shuang Wang, Hua Yue, Xiao Zhang, Guanghui Ma, Xin Li, Wei Wei
Summary: The use of bacteria and their biotic components as therapeutics has shown great potential in disease treatment. Oral delivery of bacteria is preferred due to improved patient compliance, but the harsh gastrointestinal environment reduces their viability and therapeutic efficacy. Recent advances in synthetic biology and nanotechnology have enabled the engineering of bacteria for improved survival and treatment in the gastrointestinal tract.
Article
Chemistry, Multidisciplinary
Lan Yang, Yongshun Zhang, Yu Zhang, Yani Xu, Yuai Li, Zhigiang Xie, Hairui Wang, Yunzhu Lin, Qing Lin, Tao Gong, Xun Sun, Zhirong Zhang, Ling Zhang
Summary: This study demonstrates a system for treating triple-negative breast cancer by utilizing the tumor-targeting capabilities of macrophages. By loading doxorubicin-carrying liposomes on the surfaces of macrophages, the system significantly enhances drug accumulation at tumor sites, penetrates deeper into tumor tissue, and enhances the anti-tumor immune response, leading to improved survival rates in mice.
Article
Multidisciplinary Sciences
Li-Hua Zhao, Qian He, Qingning Yuan, Yimin Gu, Xinheng He, Hong Shan, Junrui Li, Kai Wang, Yang Li, Wen Hu, Kai Wu, Jianhua Shen, H. Eric Xu
Summary: This study reports a new binding mode between a G protein and its receptor, involving a specific small-molecule agonist. The high-resolution structure of the complex is determined, providing insights for designing small-molecule agonists for class B G-protein-coupled receptors.
Article
Multidisciplinary Sciences
Li-Hua Zhao, Qian He, Qingning Yuan, Yimin Gu, Xinheng He, Hong Shan, Junrui Li, Kai Wu, Yang Li, Wen Hu, Kai Wu, Jianhua Shen, H. Eric Xu
Summary: A study reports an orally available small-molecule agonist that binds between a G protein and its receptor, and characterizes this new binding mode.
Article
Pharmacology & Pharmacy
Dan Chen, Yurong Liu, Peiwen Liu, Yang Zhou, Longguang Jiang, Cai Yuan, Mingdong Huang
Summary: Thrombosis in both macrovasculature and microvasculature is a major cause of cardiovascular diseases. This study explores the potential of protein disulfide isomerase (PDI) inhibitors as antithrombotics. The research focuses on the utilization of rutin-loaded lipid-based nano-formulation (NanoR) to improve the solubility and efficacy of rutin. The results demonstrate that NanoR exhibits enhanced antithrombotic activity and specificity, making it a promising treatment option.
Article
Gastroenterology & Hepatology
Eun Ran Kim, Jeong Su Park, Jin Hee Kim, Ji Young Oh, In Jeong Oh, Da Hyun Choi, Yu Seol Lee, I. Seul Park, SeungWon Kim, Da Hyun Lee, Jae Hee Cheon, Jin-Woo Bae, Minyoung Lee, Jin Won Cho, In Bok An, Eun Joo Nam, Sang-In Yang, Myung-Shik Lee, Soo Han Bae, Yong-ho Lee
Summary: The study suggests that a long-acting dual agonist of GLP-1 and GLP-2 receptors shows promising therapeutic effects in treating NAFLD/NASH by improving liver pathology and metabolic functions, and modulating gut microbiome composition.
Article
Engineering, Biomedical
Madhan Mohan Chandra Sekhar Jaggarapu, Abhirami Thumsi, Richard Nile, Brian D. Ridenour, Taravat Khodaei, Abhirami P. Suresh, Arezoo Esrafili, Kailong Jin, Abhinav P. Acharya
Summary: Covalent organic frameworks (COFs) are potential drug delivery biomaterials that can load and control release small molecules in a crystalline form. In this study, Kyurenine (KyH) was identified as a key metabolite that modulates T cell responses, increasing anti-inflammatory and reducing pro-inflammatory T cell frequencies. The researchers developed a method to generate imine-based COFs and loaded them with KyH, enabling controlled release for 5 days. When orally delivered to mice with rheumatoid arthritis, the KyH-loaded COFs increased anti-inflammatory T cell frequencies and decreased antibody titers. These findings demonstrate the potential of COFs as drug delivery vehicles for immune modulation.
Article
Neurosciences
Kei Oyama, Yukiko Hori, Yuji Nagai, Naohisa Miyakawa, Koki Mimura, Toshiyuki Hirabayashi, Ken-Ichi Inoue, Masahiko Takada, Makoto Higuchi, Takafumi Minamimoto
Summary: The chemogenetic technology known as DREADDs provides a reversible method for controlling neuronal activity and investigating its correlation with behavior. This study examines the pharmacokinetics and behavioral effects of the DREADD actuator Deschloroclozapine (DCZ) in macaque monkeys. The results show that orally administered DCZ can effectively manipulate neuronal activity in a chronic and reversible manner, with potential for clinical application.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Pharmacology & Pharmacy
Mo'ath Yousef, Ildiko Szabo, Jozsef Muranyi, Francoise Illien, Dora Soltesz, Csaba Bato, Gabriella Toth, Gyula Batta, Peter Nagy, Sandrine Sagan, Zoltan Banoczi
Summary: This study demonstrates the preparation and investigation of efficient cell-penetrating peptides (CPPs) and explores the possibility of using unnatural aromatic amino acids to mimic the properties of tryptophan. These highly active CPPs have been successful in delivering antitumor drugs into specific cell lines.
Article
Chemistry, Medicinal
Miguel St-Onge, Amandine Chefson, Joyce Wu, Edouard Caron-Duval, Valerie Dumais, Stephane Dorich, Jennifer Cox, Alex Caron, Jason Burch, M. David Percival, Alex G. Therien, Lee D. Fader
Summary: A property-focused optimization strategy was used to modify a class of EP4 agonists, resulting in a colon-targeted delivery prodrug with minimal exposure in the plasma. Oral administration of the prodrug showed tissue-specific activation of the EP4 receptor and modulation of immune genes in the colon. Further understanding and evaluation of this therapeutic modality is required using this tool molecule in rodent models of human disease.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Kai Chen, Yingnan Si, Jia-Shiung Guan, Zhuoxin Zhou, Seulhee Kim, Taehyun Kim, Liang Shan, Christopher D. Willey, Lufang Zhou, Xiaoguang Liu
Summary: This study developed and evaluated a new targeted therapy using highly potent natural compound verrucarin A (Ver-A) delivered with monoclonal antibody-directed extracellular vesicle (mAb-EV). The results showed that mAb-EV can penetrate the blood-brain barrier, target glioblastoma, and deliver drugs intracellularly. In vivo studies demonstrated that EGFR mAb-EV-Ver-A effectively inhibited glioblastoma growth.
Article
Engineering, Biomedical
Sunandita Sarker, Ben Wankum, Trevor Perey, Musharrat Mustaree Mau, Jeff Shimizu, Ryan Jones, Benjamin Terry
Summary: An innovative swallowable capsule has been developed for intestinal drug delivery, allowing direct injection of biological therapeutics into the small intestine wall. The capsule's design, optimization, and validation were performed through ex-vivo and in-vivo experiments, with a high success rate observed in an animal model. Drug delivery studies demonstrated the potential for systemic delivery of biologics using this capsule.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2022)
Article
Engineering, Biomedical
Raleigh M. Linville, Alexander Komin, Xiaoyan Lan, Jackson G. DeStefano, Chengyan Chu, Guanshu Liu, Piotr Walczak, Kalina Hristova, Peter C. Searson
Summary: Melittin, a membrane active peptide found in bee venom, enables transient blood-brain barrier opening for delivery of therapeutics into the brain. Studies on endothelial and neuronal viability identified the effective concentration range for BBB opening, and a tissue-engineered model was used to optimize dosing and understand the mechanism. Melittin and other membrane active variants increase paracellular permeability temporarily by disrupting cell-cell junctions.
Article
Chemistry, Multidisciplinary
Huyen Tran, Phenil J. Patel, Aktham Aburub, Andrea Sperry, Selina Estwick, Mohamed E. H. ElSayed, Amita Datta-Mannan
Summary: By combining a permeation enhancer and a peptidase inhibitor, we have identified SBTI and C10 as effective components that improve absorption and reduce degradation of therapeutic peptides in the small intestine. This formulation strategy provides a solution for oral delivery of peptide therapeutics.
PHARMACEUTICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Maria Buur Nordskov Gabe, Liv von Voss, Jenna Elizabeth Hunt, Sarina Gadgaard, Laerke Smidt Gasbjerg, Jens Juul Holst, Hannelouise Kissow, Bolette Hartmann, Mette Marie Rosenkilde
Summary: Biased GLP-2R agonists with modifications at the N-terminal have shown improved therapeutic effects on gut and bone growth. Variants like [F6A], [F6W], and [S7W] have less GLP-2R internalization and enhanced gut trophic actions, including increased small intestine weight, villus height, and crypt depth.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Stephanie E. Barrett, Marc T. Abrams, Rob Burke, Brian A. Carr, Louis S. Crocker, Robert M. Garbaccio, Bonnie J. Howell, Eric A. Kemp, Robert A. Kowtoniuk, Andrew H. Latham, Karen R. Leander, Anthony M. Leone, Mihir Patel, Sergey Pechenov, Nicole T. Pudvah, Sean Riley, Laura Sepp-Lorenzino, Eileen S. Walsh, J. Michael Williams, Steven L. Colletti
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2014)
Article
Chemistry, Multidisciplinary
Stephanie E. Barrett, Rob S. Burke, Marc T. Abrams, Carol Bason, Marina Busuek, Edward Carlini, Brian A. Carr, Louis S. Crocker, Haihong Fan, Robert M. Garbaccio, Erin N. Guidry, Jun H. Heo, Bonnie J. Howell, Eric A. Kemp, Robert A. Kowtoniuk, Andrew H. Latham, Anthony M. Leone, Michael Lyman, Rubina G. Parmar, Mihir Patel, Sergey Y. Pechenov, Tao Pei, Nicole T. Pudvah, Conrad Raab, Sean Riley, Laura Sepp-Lorenzino, Sheri Smith, Eric D. Soli, Steven Staskiewicz, Melissa Stern, Quang Truong, Marissa Vavrek, Jacob H. Waldman, Eileen S. Walsh, J. Michael Williams, Stephanie Young, Steven L. Colletti
JOURNAL OF CONTROLLED RELEASE
(2014)
Review
Chemistry, Multidisciplinary
Puneet Tyagi, Sergei Pechenov, J. Anand Subramony
JOURNAL OF CONTROLLED RELEASE
(2018)
Article
Medicine, Research & Experimental
Jacob B. Rowe, Rhiannon P. Flynn, Harrison R. Wooten, Hailey A. Noufer, Rachel A. Cancel, Jifeng Zhang, J. Anand Subramony, Sergei Pechenov, Ying Wang
MOLECULAR PHARMACEUTICS
(2018)
Article
Chemistry, Medicinal
Puneet Tyagi, Sergei Pechenov, Jonathan RiosDoria, Luke Masterson, Niall J. Dickinson, Philip Howard, Shenlan Mao, Jay Harper, Leslie Wetzel, Kwok Yo, J. Anand Subramony
JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Article
Biophysics
Jacob B. Rowe, Rachel A. Cancel, Tyler D. Evangelous, Rhiannon P. Flynn, Sergei Pechenov, J. Anand Subramony, Jifeng Zhang, Ying Wang
BIOPHYSICAL JOURNAL
(2017)
Review
Pharmacology & Pharmacy
Sergei Pechenov, Himanshu Bhattacharjee, Daniel Yin, Sachin Mittal, J. Anand Subramony
ADVANCED DRUG DELIVERY REVIEWS
(2017)
Article
Chemistry, Multidisciplinary
S Pechenov, B Shenoy, MX Yang, SK Basu, AL Margolin
JOURNAL OF CONTROLLED RELEASE
(2004)
Article
Multidisciplinary Sciences
MX Yang, B Shenoy, M Disttler, R Patel, M McGrath, S Pechenov, AL Margolin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2003)
Article
Biochemical Research Methods
RV Tikhonov, SE Pechenov, IA Belacheu, SA Yakimov, VE Klyushnichenko, H Tunes, JE Thiemann, L Vilela, AN Wulfson
PROTEIN EXPRESSION AND PURIFICATION
(2002)
Article
Biochemical Research Methods
RV Tikhonov, SE Pechenov, IA Belacheu, SA Yakimov, VE Klyushnichenko, EF Boldireva, VG Korobko, H Tunes, JE Thiemann, L Vilela, AN Wulfson
PROTEIN EXPRESSION AND PURIFICATION
(2001)