期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-99939-w
关键词
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资金
- Dutch Cancer Society
- Stichting Erasmus Heelkundig Kankeronderzoek
- Vereniging Trustfonds Erasmus University Rotterdam
Researchers introduced a method combining high resolution intravital imaging and whole-mount histology to study the spatiotemporal dynamics of endothelial cells and pericytes. This method identified blood flow patterns, spatial subtypes, and molecular expression of these vascular cells, enabling a 4D dissection of their association at the molecular level.
Endothelial cells and pericytes are highly dynamic vascular cells and several subtypes, based on their spatiotemporal dynamics or molecular expression, are believed to exist. The interaction between endothelial cells and pericytes is of importance in many aspects ranging from basic development to diseases like cancer. Identification of spatiotemporal dynamics is particularly interesting and methods to studies these are in demand. Here we describe the technical details of a method combining the benefits of high resolution intravital imaging and whole-mount histology. With intravital imaging using an adapted light weight dorsal skinfold chamber we identified blood flow patterns and spatiotemporal subtypes of endothelial cells and pericytes in a 4D (XYZ, spatial+T, time dimension) manner as representative examples for this model. Thereafter the tissue was extracted and stained as a whole-mount, by which the position and volumetric space of endothelial cells as well as pericytes were maintained, to identify molecular subtypes. Integration of the two imaging methods enabled 4D dissection of endothelial cell-pericyte association at the molecular level.
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