期刊
VIRUSES-BASEL
卷 14, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/v14010053
关键词
African swine fever virus (ASFV); I267L; deletion; virulence; replication
类别
资金
- National Key R&D Program of China [2021YFD1801400]
- National Natural Science Foundation of China [U19A2039]
The I267L gene in ASFV is identified as a conserved gene that is expressed early in infection. Deletion of the I267L gene does not impact the replication or virulence of ASFV. Using a fluorescent-tagged mutant virus can provide visual results in related research.
African swine fever virus (ASFV) is the causative agent of African swine fever (ASF) which reaches up to 100% case fatality in domestic pigs and wild boar and causes significant economic losses in the swine industry. Lack of knowledge of the function of ASFV genes is a serious impediment to the development of the safe and effective vaccine. Herein, I267L was identified as a relative conserved gene and an early expressed gene. A recombinant virus (SY18 Delta I267L) with I267L gene deletion was produced by replacing I267L of the virulent ASFV SY18 with enhanced green fluorescent protein (EGFP) cassette. The replication kinetics of SY18 Delta I267L is similar to that of the parental isolate in vitro. Moreover, the doses of 10(2.0) TCID50 (n = 5) and 10(5.0) TCID50 (n = 5) SY18 Delta I267L caused virulent phenotype, severe clinical signs, viremia, high viral load, and mortality in domestic pigs inoculated intramuscularly as the virulent parental virus strain. Therefore, the deletion of I267L does not affect the replication or the virulence of ASFV. Utilizing the fluorescent-tagged virulence deletant can be easy to gain a visual result in related research such as the inactivation effect of some drugs, disinfectants, extracts, etc. on ASFV.
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