Article
Immunology
Dongdong Yin, Lei Yin, Hao Guo, Jieru Wang, Xuehuai Shen, Ruihong Zhao, Xiaocheng Pan, Yin Dai
Summary: A rapid diagnostic method for detecting PEDV was developed in this study, combining the CRISPR/Cas13a system with recombinase aided amplification. The method achieved differentiation between wild-type strains and attenuated vaccine strains. It showed high sensitivity and specificity, with visual readout for result determination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Yang Li, Jiajing Shang, Juan Luo, Fuyou Zhang, Ge Meng, Yingjie Feng, Wenming Jiang, Xiaohui Yu, Chunran Deng, Guanhui Liu, Hualei Liu
Summary: In this study, a convenient, efficient, and accurate method for detecting H5-AIV avian influenza virus was established. The process can be completed within 40 minutes and has a detection limit comparable to RT-qPCR. The method showed no cross-reactivity with other viruses. Results can be visualized and interpreted using lateral flow dipstick, making it suitable for use in grassroots laboratories and field-deployable assays.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jieru Wang, Xiaojie Zhu, Dongdong Yin, Chang Cai, Hailong Liu, Yuqing Yang, Zishi Guo, Lei Yin, Xuehuai Shen, Yin Dai, Xiaocheng Pan
Summary: First identified in China in 2019, porcine circovirus (PCV4) now poses a threat to pig health and the pig industry in various Chinese provinces and South Korea. To control the spread of infection, early detection of PCV4 is crucial. Current detection methods are labor-intensive and time-consuming. Therefore, a simple and rapid testing kit using CRISPR and Cas13a technology was developed.
Article
Agriculture, Dairy & Animal Science
Desheng Zhang, Sen Jiang, Nengwen Xia, Youwen Zhang, Jiajia Zhang, Anjing Liu, Chenyang Zhang, Nanhua Chen, Francois Meurens, Wanglong Zheng, Jianzhong Zhu
Summary: In this study, a simple, rapid, sensitive, and specific visual detection method for African swine fever virus (ASFV) was developed, demonstrating its potential for on-site detection of ASFV.
Article
Biochemical Research Methods
Ning Wei, Junyao Xiong, Junheng Ma, Jing Ye, Youhui Si, Shengbo Cao
Summary: In this study, an efficient, sensitive, and specific detection method for EMCV was developed using CRISPR/Cas13a and RAA technology. The isothermal detection at 37 degrees Celsius allowed visual observation by the naked eye. The method showed high sensitivity, excellent specificity, and simplicity and affordability in operation.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Chemistry, Analytical
Yanju Chen, Siwenjie Qian, Jian Wu, Xiaoping Zhong, Meixia Wang, Jin-song He, Xiaoping Yu
Summary: In this study, a microchip-assisted detection platform was proposed for the detection of African swine fever virus (ASFV). The liquid transfer between different reaction chambers of the microchip is driven by the gravity of liquid solutions, eliminating the need for complex liquid transfer and instruments. The platform has the advantages of low cost, high efficiency, and easy operation, making it promising for popularization in low-resourced areas.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Immunology
Yuan Tian, Zihao Fan, Ling Xu, Yaling Cao, Sisi Chen, Zhenzhen Pan, Yao Gao, Hao Li, Sujun Zheng, Yingmin Ma, Zhongping Duan, Xiangying Zhang, Feng Ren
Summary: A colloidal gold test strip was developed for the detection of hepatitis B virus (HBV) DNA based on CRISPR/Cas13a and RAA technology. It demonstrated high sensitivity and specificity, effectively identifying HBV-infected patients and dynamic plasma samples of patients on antiviral therapy. This study provides a visual and faster alternative to current PCR-based diagnosis for HBV infection.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Veterinary Sciences
Chao Qin, Jiajia Liu, Wenqi Zhu, Muchu Zeng, Ke Xu, Jinmei Ding, Hao Zhou, Jianshen Zhu, Yuqing Ke, Lai Yan Li, Gaoyuan Sheng, Zhuoru Li, Huaixi Luo, Shengyao Jiang, Kangchun Chen, Xianting Ding, He Meng
Summary: African swine fever virus (ASFV) is a major cause of worldwide agricultural loss, and conventional detection methods are time-consuming and instrument-dependent. In this study, a one-pot visual detection system for ASFV using CRISPR/Cas12a technology combined with LAMP or RPA was developed. The system allows rapid, sensitive, and specific detection of ASFV and can be observed by the naked eye, making it suitable for point-of-care testing.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Huimin Wang, Ailing Su, Chengxin Bao, Chongyang Liang, Weiqing Xu, Jingjing Chang, Shuping Xu
Summary: A surface-enhanced Raman scattering (SERS) strategy combined with a CRISPR/Cas12a system is designed for amplification-free gene detection of African swine fever virus (ASFV). The method achieves high sensitivity and selectivity in liquid-phase detection, and is mainly used for on-site inspection and quick testing of gene samples.
Article
Virology
Hongbo Liu, Shuailei Chang, Sijia Chen, Yue Du, Hui Wang, Chao Wang, Ying Xiang, Qi Wang, Zhenjun Li, Shengqi Wang, Shaofu Qiu, Hongbin Song
Summary: A CRISPR/Cas-based detection method was developed to rapidly identify individuals infected with SARS-CoV-2 and control the spread of COVID-19. The method utilizes multienzyme isothermal amplification, CRISPR-Cas13a nuclease, and LFA, achieving high sensitivity and reliability.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Gaihua Cao, Yifan Xiong, Fuping Nie, Xiaolong Chen, Lan Peng, Yingguo Li, Mei Yang, Danqun Huo, Changjun Hou
Summary: A fluorescent biosensor and lateral flow assay strip based on PCR and CRISPR/Cas12a system were developed for the detection of African swine fever virus (ASFV). The biosensor exhibited high sensitivity and specificity, and showed comparable accuracy to traditional qPCR in point-of-care testing.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Gaihua Cao, Danqun Huo, Xiaolong Chen, Xianfeng Wang, Shiying Zhou, Shixian Zhao, Xiaogang Luo, Changjun Hou
Summary: This study developed a biosensor based on CRISPR/Cas13a and RPA technology to detect the S and Orf1ab genes of SARS-CoV-2 quickly and accurately. Additionally, an automated, portable fluorescence analyzer and lateral flow strip were developed to provide more accurate and high-throughput detection results during on-site testing.
Article
Biochemical Research Methods
Xiaoyun Wen, Qingyun Xie, Jiahao Li, Yanrui Pei, Yun Bai, Fei Liu, Haiying Cui, Guoqing Shao, Zhixin Feng
Summary: In this study, a rapid ASFV detection method in pork using fluorescent test strip combined with RAA and QDMs was established. The method showed high specificity, sensitivity, and short detection time, providing a potential tool for maintaining the food safety of pork products.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Yuhang Zhang, Qingmei Li, Ruining Wang, Li Wang, Xun Wang, Jun Luo, Guangxu Xing, Guanmin Zheng, Bo Wan, Junqing Guo, Gaiping Zhang
Summary: Classical swine fever (CSF) is a notifiable animal disease causing significant economic losses to the swine industry worldwide. Differentiation of infected and vaccinated pigs is a crucial step for eradication, and this study establishes a diagnostic platform based on CRISPR/Cas13a for precise differentiation of virulent and vaccine strains.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemical Research Methods
Thi Thu Hang Vu, Van Phan Le, Dae Gwin Jeong, Minjoo Yeom, Jinsik Oh, Bokyu Kang, Song-Kyu Park, Daesub Song
Summary: In this study, a lateral flow assay was developed for on-site detection of African swine fever virus (ASFV). The assay demonstrated fast, high sensitivity, and high specificity, capable of detecting the virus from samples within 20 minutes. It can be applied to clinical samples and outbreak detection, holding significant clinical value.
JOURNAL OF VIROLOGICAL METHODS
(2023)