期刊
VIROLOGY
卷 566, 期 -, 页码 89-97出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2021.12.001
关键词
Norovirus; Virus-like particle; VLP; Platform; Vaccine; HA2; M2e; Influenza virus; SpyTag; SpyCatcher
类别
资金
- Academy of Finland [309455, 335870]
- Competitive State Research Financing of the Expert Responsibility Area of Fimlab Laboratories
- Cancer Society of Finland
- Academy of Finland (AKA) [309455, 335870, 335870, 309455] Funding Source: Academy of Finland (AKA)
NoV VLP platform demonstrates the potential to deliver foreign antigens and serve as a vaccine carrier, showing good antigen induction capacity and receptor binding activity.
Virus-like particles (VLPs) modified through different molecular technologies are employed as delivery vehicles or platforms for heterologous antigen display. We have recently created a norovirus (NoV) VLP platform, where two influenza antigens, the extracellular domain of matrix protein M2 (M2e) or the stem domain of the major envelope glycoprotein hemagglutinin (HA2) are displayed on the surface of the NoV VLPs by SpyTag/SpyCatcher conjugation. To demonstrate the feasibility of the platform to deliver foreign antigens, this study examined potential interference of the conjugation with induction of antibodies against conjugated M2e peptide, HA2, and NoV VLP carrier. High antibody response was induced by HA2 but not M2e decorated VLPs. Furthermore, HA2elicited antibodies did not neutralize the homologous influenza virus in vitro. Conjugated NoV VLPs retained intact receptor binding capacity and self-immunogenicity. The results demonstrate that NoV VLPs could be simultaneously used as a platform to deliver foreign antigens and a NoV vaccine.
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