期刊
VETERINARY PARASITOLOGY
卷 298, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2021.109517
关键词
Fasciola hepatica; Diagnostics; ELISA; Coproantigen; Cathepsin L peptidases
资金
- European Research Council Advanced Grant (HELIVAC) [322725]
- Science Foundation Ireland (SFI) [17/RP/5368]
- Science Foundation Ireland (SFI) [17/RP/5368] Funding Source: Science Foundation Ireland (SFI)
- European Research Council (ERC) [322725] Funding Source: European Research Council (ERC)
Fasciolosis, a parasitic disease of agricultural livestock caused by the liver fluke Fasciola hepatica, is traditionally diagnosed using laborious methods with low sensitivity. Enzyme linked immunosorbent assays (ELISA) offer a faster and more sensitive alternative for diagnosis, with recombinant cathepsin L proteins showing promise as effective antigens in detecting liver fluke infection in sheep.
Fasciolosis, a global parasitic disease of agricultural livestock, is caused by the liver fluke Fasciola hepatica. Management and strategic control of fasciolosis on farms depends on early assessment of the extent of disease so that control measures can be implemented quickly. Traditionally, this has relied on the detection of eggs in the faeces of animals, a laborious method that lacks sensitivity, especially for sub-clinical infections, and identifies chronic infections only. Enzyme linked immunosorbent assays (ELISA) offer a quicker and more sensitive serological means of diagnosis that could detect early acute infection before significant liver damage occurs. The performance of three functionally-active recombinant forms of the major F. hepatica secreted cathepsins L, rFhCL1, rFhCL2, rFhCL3, and a cathepsin B, rFhCB3, were evaluated as antigens in an indirect ELISA to sem-logically diagnose liver fluke infection in experimentally and naturally infected sheep. rFhCL1 and rFhCL3 were the most effective of the four antigens detecting fasciolosis in sheep as early as three weeks after experimental infection, at least five weeks earlier than both copmantigen and faecal egg tests. In addition, the rFhCL1 and rFhCL3 ELISAs had a very low detection limit for liver fluke in lambs exposed to natural infection on pastures and thus could play a major role in the surveillance of farms and a 'test and treat' approach to disease management. Finally, antibodies to all three cathepsin L proteases remain high throughout chronic infection but decline rapidly after drug treatment with the flukicide, triclabendazole, implying that the test may be adapted to trace the effectiveness of drug treatment.
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