期刊
TRENDS IN PLANT SCIENCE
卷 27, 期 9, 页码 890-907出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tplants.2022.01.009
关键词
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资金
- National Natural Science Foundation of China [32170276, 32001456, 31620103912]
- Major International (Regional) Joint Research Project from NSFC-ASRT [32061143044]
- Australian Research Council [FT210100366, DE1401011143]
- Horticulture Innovation Australia [VG17003, LP18000]
This article summarizes the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes, while also reviewing the application of CRISPR/Cas in plants. Revealing the functions of miRNAs and manipulating them with CRISPR will lead to a deeper understanding of plant miRNA functions and more sustainable genetic engineering.
Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.
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