Article
Reproductive Biology
Huihui Gao, Heran Cao, Zhenpeng Li, Long Li, Yingjie Guo, Yining Chen, Guofan Peng, Wenxian Zeng, Jian Du, Wuzi Dong, Fangxia Yang
Summary: Spermatogenesis is a complex process regulated by endocrine and paracrine factors, with Sertoli cells playing a crucial role. The molecular pathways of Sertoli cell action in spermatogenesis remain controversial. Recent studies have focused on exosomes as a mechanism of intercellular communication.
Article
Medicine, Research & Experimental
Fatemeh Sokouti Nasimi, Saber Zahri, Shahin Ahmadian, Afsaneh Bagherzadeh, Nahideh Nazdikbin Yamchi, Leila Haghighi, Alberto Miranda Bedate, Balal Khalilzadeh, Reza Rahbarghazi, Mahdi Mahdipour
Summary: The study showed that estradiol can increase the viability of mouse SSCs in a dose-dependent manner, leading to changes in cell morphology and gene expression, as well as reduced sperm parameters and testicular progenitor cells. This suggests that estradiol may induce Sertoli cell differentiation of SSCs while long-term exogenous administration can lead to infertility.
Article
Veterinary Sciences
Xuejiao An, Qiao Li, Nana Chen, Taotao Li, Huihui Wang, Manchun Su, Huibin Shi, Youji Ma
Summary: This study established a co-culture system of SCs and SSCs and identified a list of genes and small metabolic molecules that affect the proliferation and differentiation of SSCs. The findings provide additional insights into the regulatory mechanisms underlying interactions between SCs and SSCs during mammalian spermatogenesis.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Cell Biology
Mengdong Liu, Yunshu Yang, Bin Zhao, Yuefan Yang, Jing Wang, Kuo Shen, Xuekang Yang, Dahai Hu, Guoxu Zheng, Juntao Han
Summary: The study showed that ADMSC-Exos reduced oxidative stress, inflammation, and microglial infiltration by activating the SIRT1 pathway, providing protective effects against radiation-induced brain injury.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Wei Chen, Yinghong Cui, Minqi Ning, Haorui Zhang, Chenjun Yin, Zuping He
Summary: This review discusses the important functions and mechanisms of microRNAs (miRNAs) in regulating the self-renewal and apoptosis of human spermatogonial stem cells (SSCs) and Sertoli cells. It also describes the regulatory networks of miRNAs in controlling the decisions of Sertoli cells and suggests future research directions. This review provides new molecular targets and insights into the epigenetic regulation of human spermatogenesis for the treatment of male infertility.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Reproductive Biology
Jing Li, Bo-Xing Sun, Da-Li Wang, Ying Liu, Jia-Jia Qi, Xiao-Wei Nie, Chun-Yan Bai, Jia-Bao Zhang, Shuang Liang
Summary: The study revealed that cypermethrin (CYP) induced cytotoxicity in porcine Sertoli cells (SCs) through oxidative stress, DNA damage, and apoptosis, and melatonin showed a protective effect against CYP-induced toxicity.
Review
Endocrinology & Metabolism
William W. Wright
Summary: This review focuses on the in vivo regulation of spermatogonial stem cells (SSCs) in adult testes by glial cell line-derived neurotrophic factor (GDNF). Through chemical-genetic approach, the inhibition of GDNF stimulation of SSCs was found to decrease replication and increase differentiation of SSCs, ultimately leading to the loss of all SSCs and resembling the condition of Sertoli cell-only (SCO) syndrome. Abnormally low expression of GDNF, FGF2, and FGF8 was observed in infertile human testes, although some SCO testes still contain SSCs. Restoration of GDNF signaling showed partial rebuilding of depleted SSC pool, suggesting GDNF as a potential therapy for men with SCO syndrome.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yaxing Kang, Yiran Song, Yuxin Luo, Jia Song, Chenyang Li, Shuangshuang Yang, Jinbo Guo, Jun Yu, Xiaolan Zhang
Summary: The study showed that exosomes derived from human umbilical cord mesenchymal stem cells (hUC-MSCs) protect against non-alcoholic steatohepatitis (NASH) through the Nrf2/NQO-1 pathway, exerting antioxidant and anti-inflammatory effects.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Environmental Sciences
Weiqi Sun, Zhijing Ni, Rui Li, Xiuli Chang, Weihua Li, Mingjun Yang, Zhijun Zhou
Summary: The study showed that flurochloridone induces apoptosis in TM4 cells through the mitochondrial apoptotic pathway, accompanied by accumulation of ROS, depolarization of the mitochondrial membrane potential, etc. Pretreatment with the ROS scavenger NAC significantly alleviated flurochloridone-induced cell apoptosis and MMP depolarization.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Pharmacology & Pharmacy
Yasemin Aydin, Banu Orta-Yilmaz
Summary: Excessive intake of arsenic and fluoride in underground drinking water has adverse effects on the male reproductive system, including reduced cell viability and proliferation, increased cytotoxicity, and induction of oxidative stress and apoptosis. The combined exposure of arsenic and fluoride has a synergistic effect, highlighting the need to reduce their exposure to prevent male infertility.
Article
Neurosciences
Xiaotang Ma, Jia Zhao, Suqing Li, Yan Wang, Jinhua Liu, Yumeng Shi, Jiehong Liu, Yanyu Chen, Yanfang Chen, Qunwen Pan
Summary: The study demonstrates that Rab27a regulates brain EXs secretion and functions, contributing to cerebral vascular protection from ischemic insult by preventing oxidative stress and apoptosis via down-regulating NOX2 and cleaved caspase-3 expression.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Environmental Sciences
Xinyu Liu, Huaming Xi, Shuaiqi Han, Hongyun Zhang, Jianhong Hu
Summary: This study found that exposure to ZEA can induce oxidative stress and autophagy in dairy goat Sertoli cells, leading to cell apoptosis and decreased cell viability. NAC pretreatment can effectively inhibit ZEA-induced oxidative stress, apoptosis, and autophagy. These findings contribute to a better understanding of the mechanisms of ZEA-induced reproductive damage in dairy goats.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Cell Biology
Dai Zhou, Jingyu Fan, Zhizhong Liu, Ruiling Tang, Xingming Wang, Hao Bo, Fang Zhu, Xueheng Zhao, Zenghui Huang, Liu Xing, Ke Tao, Han Zhang, Hongchuan Nie, Huan Zhang, Wenbing Zhu, Zuping He, Liqing Fan
Summary: This study reveals that TCF3 regulates the proliferation and apoptosis of human SSCs via targeting PODXL, providing a novel molecular mechanism for understanding the fate determinations of human SSCs and potential new targets for gene therapy of male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Dai Zhou, Jingyu Fan, Zhizhong Liu, Ruiling Tang, Xingming Wang, Hao Bo, Fang Zhu, Xueheng Zhao, Zenghui Huang, Liu Xing, Ke Tao, Han Zhang, Hongchuan Nie, Huan Zhang, Wenbing Zhu, Zuping He, Liqing Fan
Summary: The gene TCF3 was found to promote proliferation and inhibit apoptosis of human SSCs by targeting PODXL, offering new targets for gene therapy of male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ilenia Martinelli, Mathieu Cinato, Sokhna Keita, Dimitri Marsal, Valentin Antoszewski, Junwu Tao, Oksana Kunduzova
Summary: The study found that short-term exposure to EMF did not have a significant effect on the myocardial antioxidant defense system and apoptotic cell death in cardiac cells and tissues.
Article
Engineering, Chemical
R. Mehrabifard, H. Mehdian, K. Hajisharifi, E. Amini
PLASMA CHEMISTRY AND PLASMA PROCESSING
(2020)
Article
Oncology
Elaheh Amini, Mohammad Nabiuni, Seyed Bahram Behzad, Danial Seyfi, Farhad Eisvand, Amirhossein Sahebkar, Abolfazl Shakeri
RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY
(2020)
Article
Physics, Applied
Milad Rasouli, Hassan Mehdian, Kamal Hajisharifi, Elaheh Amini, Kostya (Ken) Ostrikov, Eric Robert
Summary: The study demonstrates the high potential of plasma-activated medium in sensitizing ovarian cancer cells to carboplatin, inducing apoptosis, and inhibiting antioxidant genes. Selective mechanisms were related to the pH and concentration of reactive species in the plasma-treated medium.
PLASMA PROCESSES AND POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Mohsen Sheykhhasan, Naser Kalhor, Azar Sheikholeslami, Masoumeh Dolati, Elaheh Amini, Hoda Fazaeli
Summary: This study investigated the role of exosomes derived from adipose tissue-derived mesenchymal stem cells in transfecting miR-145 into breast cancer cells to inhibit their proliferation and metastasis. The results showed that exosome-mediated delivery of miR-145 improved its anticancer properties, suggesting a potential novel therapeutic strategy for breast cancer in the future.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Anatomy & Morphology
Ghazal Ghajari, Mohammad Nabiuni, Elaheh Amini
Summary: The experiment demonstrated that Ganoderma lucidum extract can prevent lithium carbonate-induced testicular toxicity and related lesions by inhibiting oxidative stress and repairing damages, thus protecting the health of the male reproductive system.
Article
Pharmacology & Pharmacy
Farima Rahimi Mansour, Mohammad Nabiuni, Elaheh Amini
Summary: Aluminum, a widely used metal, can affect the ovaries and granulosa cells by up-regulating steroidogenic and proliferative genes. Additionally, aluminum may induce issues such as apoptosis and autophagy.
Article
Pharmacology & Pharmacy
Mona Sadeghizade, Javad Baharara, Farzaneh Salek, Elaheh Amini
Summary: This study aimed to investigate the effect of Ficus carica leaf extract on apoptosis induction in B16F10 melanoma cancer cells. The results showed that the extract inhibited cell proliferation and promoted apoptosis in the melanoma cells. This research provides new insights for the treatment of melanoma.
JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS
(2022)
Review
Medicine, Research & Experimental
Hoda Fazaeli, Azar Sheikholeslami, Fatemeh Ghasemian, Elaheh Amini, Mohsen Sheykhhasan
Summary: Long noncoding RNAs (lncRNAs) have been recognized as vital regulators of tumor establishment and are frequently dysregulated in various cancers. FENDRR (fetal-lethal noncoding developmental regulatory RNA) serves as a key lncRNA in multiple malignancies, playing a crucial role in organ development and influencing signaling pathways in different cancers. Moreover, FENDRR has been found to modulate the development and progression of several cancers, as well as epigenetic regulation of target genes involved in tumor immunity. This review highlights the biological functions and molecular mechanisms of FENDRR in different cancers, emphasizing its potential as a cancer biomarker and a potential therapeutic target.
CURRENT MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Azim Ziyaei Halimehjani, Faezeh Dehghan, Vida Tafakori, Elaheh Amini, Seyyed Emad Hooshmand, Yazdanbkhsh Lotfi Nosood
Summary: A metal-free multicomponent synthetic route was developed for the preparation of dithiocarbamate-containing piperazine derivatives through the C-N bond cleavage of DABCO ring. The method is efficient and offers high yields, and the synthesized compounds exhibit good antibacterial and antifungal activities.
Review
Medicine, Research & Experimental
Narjes Amiri, Paria Mohammadi, Atefeh Allahgholi, Farzaneh Salek, Elaheh Amini
Summary: In the male reproductive system, the seminiferous tubules contain spermatogenic cells and sertoli cells (SCs) that play important roles in supporting spermatogenesis and regulating reproductive capacity. SC-based exosomes, which are produced by SCs, have emerged as potential biomarkers and cell-free therapies in male reproductive diseases. Understanding the signaling pathways and key molecules involved in the interaction between SCs and other cells can lead to diagnostic and regenerative targets for male infertility.
Article
Pharmacology & Pharmacy
Shirin Ahmadi, Mohammad Nabiuni, Mohammad Tahmaseb, Elaheh Amini
Summary: The study revealed that Lithium and Crocin can enhance the expression of neurotrophic factors BDNF and GDNF in EPI-NCSCs, supporting their neuroprotective/neurogenesis effects. This suggests a potential for using these substances in cell therapy and combination therapy for neurodegenerative diseases.
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2021)
Article
Fisheries
J. Baharara, E. Amini, F. Salek
IRANIAN JOURNAL OF FISHERIES SCIENCES
(2020)
Article
Neurosciences
Zahra Baharvand, Mohammad Nabiuni, Mohammad Tahmaseb, Elaheh Amini, Sareh Pandamooz
ACTA NEUROBIOLOGIAE EXPERIMENTALIS
(2020)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
