期刊
SMALL
卷 18, 期 14, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202107963
关键词
heat resistance; intracellular temperatures; luminescence; nanothermometers; real-time monitoring
类别
资金
- Shanghai Sci. Tech. Comm. [21ZR1403900]
- Innovative research team of high-level local universities in Shanghai
- Education Commission of Shanghai Municipality [ZDSYS14005]
- National Natural Science Foundation of China [22101257]
In this study, a highly stable and sensitive thermal probe was developed using nanotechnology to observe temperature changes within cells. The results show that intracellular temperature has a resistance mechanism to external stimuli and tends to approach a favorable temperature.
Temperature as a typical parameter, which influences the status of living creatures, is essential to life activities and indicates the initial cellular activities. In recent years, the rapid development of nanotechnology provides a new tool for studying temperature variation at the micro- or nano-scales. In this study, an important phenomenon is observed at the cell level using luminescent probes to explore intracellular temperature changes, based on Yb-Er doping nanoparticles with special upconversion readout mode and intensity ratio signals (I-525 and I-545). Further optimization of this four-layer core-shell ratio nanothermometer endows it with remarkable characteristics: super photostability, sensitivity, and protection owing to the shell. Thus this kind of thermal probe has the property of anti-interference to the complex chemical environment, responding exclusively to temperature, when it is used in liquid and cells to reflect external temperature changes at the nanoscale. The intracellular temperature of living RAW and CAOV3 cells are observed to have a resistance mechanism to external stimuli and approach a more favorable temperature, especially for CAOV3 cells with good heat resistance, with the intracellular temperature 4.8 degrees C higher than incubated medium under 5 degrees C environment, and 4.4 degrees C lower than the medium under 60 degrees C environment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据