Article
Biochemistry & Molecular Biology
Hector Esquer, Qiong Zhou, Travis Nemkov, Adedoyin D. Abraham, Sebastien Rinaldetti, Yu-Chi Chen, Xiaohu Zhang, Michael Orman, Angelo D'Alessandro, Marc Ferrer, Wells A. Messersmith, Daniel LaBarbera
Summary: The translation describes the important role of epithelial-mesenchymal transition (EMT) in promoting cancer, as well as a dual-reporter system for identifying and isolating different cell phenotypes. This tool enables the study and induction of EMT and MET processes, providing insights into mesenchymal cell plasticity.
Review
Oncology
Barbora Kvokackova, Jan Remsik, Mohit Kumar Jolly, Karel Soucek
Summary: Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) play critical roles in the development of TNBC, shaping aggressive and heterogeneous biological features, but may also contribute to the progression of metastatic disease.
Article
Cell Biology
Wen Jia, Mohit Kumar Jolly, Herbert Levine
Summary: The epithelial-mesenchymal transition (EMT) is a crucial cellular process for wound healing, cancer metastasis, and embryonic development. Recent studies have shown that hybrid epithelial/mesenchymal states, possessing both epithelial and mesenchymal traits, play a significant role in cancer metastasis and resistance to therapies. NRF2 has been identified as a stabilizing factor for these hybrid states. This research incorporates a phenomenological epigenetic feedback effect into a computational model for EMT signaling and demonstrates its stabilizing effect on the hybrid state if NRF2 influences SNAIL at an epigenetic level.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Ayalur Raghu Subbalakshmi, Sarthak Sahoo, Prakruthi Manjunatha, Shaurya Goyal, Vignesh A. Kasiviswanathan, Yeshwanth Mahesh, Soundharya Ramu, Isabelle McMullen, Jason A. A. Somarelli, Mohit Kumar Jolly
Summary: ELF3 is a factor strongly associated with an epithelial phenotype and is inhibited during EMT. It inhibits the progression of EMT and can counteract EMT induction even in the presence of EMT-inducing factors. The prognostic capacity of ELF3 is specific to cell-of-origin or lineage.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Review
Oncology
Xiaobo Zheng, Fuzhen Dai, Lei Feng, Hong Zou, Li Feng, Mingqing Xu
Summary: The interplay between epithelial-mesenchymal plasticity and cancer stem cells plays a crucial role in cancer progression, affecting the aggressiveness of tumor cells and their response to treatment. Understanding the dynamic nature of EMT as a hybrid state rather than a binary model is important for unraveling the complexities of cancer biology and developing targeted therapeutic interventions.
FRONTIERS IN ONCOLOGY
(2021)
Review
Oncology
Jaewang Lee, Jong-Lyel Roh
Summary: Cancers that have transitioned to a mesenchymal state are resistant to conventional therapies. This transition affects lipid metabolism and increases polyunsaturated fatty acids in cancer cells, resulting in chemo- and radio-resistance. The altered metabolism in cancer cells enables invasion and metastasis but also makes them susceptible to lipid peroxidation under oxidative stress. Ferroptosis is highly effective against mesenchymal cancer cells, and targeting the lipid peroxidase pathway can kill therapy-resistant cells. This article discusses the regulatory mechanisms of ferroptosis in cancer and its implications for effective cancer therapy.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2023)
Review
Oncology
Cosima C. Hoch, Fabian Stoegbauer, Barbara Wollenberg
Summary: Salivary adenoid cystic carcinoma (SACC) is an aggressive tumor with a high tendency for metastasis. Epithelial-mesenchymal transition (EMT) plays a crucial role in the invasiveness of SACC by enhancing mobility and invasiveness. This manuscript reviews the latest research on potential biomarkers of EMT in SACC, aiming to identify new therapeutic targets and improve patient management.
Review
Pharmacology & Pharmacy
Sara Lovisa
Summary: EMT, a process crucial in wound healing, fibrosis, and cancer, has gained significant attention from researchers since its discovery in the seventies. Despite advances in understanding its functional role, targeting EMT in fibrotic diseases remains a challenge at an early stage of development.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Juliane Strietz, Stella S. Stepputtis, Marie Follo, Peter Bronsert, Elmar Stickeler, Jochen Maurer
Summary: Primary breast cancer stem cell (BCSC) cultures isolated from TNBC specimens have been established and shown to grow as tumor spheres under anchorage-independent culture conditions in vitro, while reliably forming tumors in mice when transplanted in limiting dilutions in vivo. Additionally, these BCSC xenograft tumors exhibit similar architecture and gene expression to the original patient tumor, suggesting a promising model for researching BCSC biology and testing new treatment options for TNBC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Ayalur Raghu Subbalakshmi, Sarthak Sahoo, Isabelle McMullen, Aaditya Narayan Saxena, Sudhanva Kalasapura Venugopal, Jason A. Somarelli, Mohit Kumar Jolly
Summary: Research shows that the transcription factor KLF4 can delay the onset of EMT by suppressing multiple EMT-TFs and promote a phenotypic shift towards a more epithelial state. KLF4 can also strengthen its influence on modulating EMT dynamics by inhibiting cell-state transitions at the epigenetic level.
Review
Oncology
Qiu-Luo Liu, Maochao Luo, Canhua Huang, Hai-Ning Chen, Zong-Guang Zhou
Summary: Metastasis is the final stage of cancer progression, driven by a multistep process involving genetic and/or epigenetic alterations in cancer cells. This process leads to subsequent transformation of metastatic cells.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Andrea Abaurrea, Angela M. Araujo, Maria M. Caffarel
Summary: Epithelial-mesenchymal plasticity (EMP) is crucial in cancer development, promoting the acquisition of mesenchymal features in cancer cells through cytokine signal transduction pathways, such as the interleukin 6 (IL-6) family. However, the effect may depend heavily on the context.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yuxin Tong, Yifei Qi, Gaofeng Xiong, Junyan Li, Timothy L. Scott, Jie Chen, Daheng He, Linzhang Li, Chi Wang, Andrew N. Lane, Ren Xu
Summary: Accumulation of succinate is associated with the progression of cancer. Succinate induces the mesenchymal phenotypes in mammary epithelial cells and enhances cancer cell stemness. Elevated succinate levels reduce the accumulation of 5-hydroxymethylcytosine and lead to the transcriptional repression of EMT-related genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Oncology
Marie-Lucie Racu, Laetitia Lebrun, Andrea Alex Schiavo, Claude Van Campenhout, Sarah De Clercq, Lara Absil, Esmeralda Minguijon Perez, Calliope Maris, Christine Decaestecker, Isabelle Salmon, Nicky D'Haene
Summary: Pancreatic ductal adenocarcinoma (PDAC) has a low survival rate and its incidence is increasing over time. Understanding the molecular mechanisms behind metastasis and chemoresistance in PDAC is crucial, as these are the main causes of death in patients. SMAD4 is deactivated in half of PDAC cases and its loss is associated with worse overall survival and metastasis. SMAD4 is a key transducer in the TGF-beta pathway, which plays a role in epithelial-mesenchymal transition (EMT). EMT is a biological process where epithelial cells lose their characteristics and acquire a more mesenchymal phenotype, leading to increased motility. Recent studies suggest that cells may undergo intermediate states during EMT, known as epithelial-mesenchymal plasticity (EMP), which exhibit enhanced aggressiveness and more efficient metastasis. This review aims to summarize and analyze current knowledge on SMAD4 loss in PDAC patients and investigate its potential role in EMP, in order to better understand its function in PDAC carcinogenesis.
Review
Anatomy & Morphology
Mohit Kumar Jolly, Ryan J. Murphy, Sugandha Bhatia, Holly J. Whitfield, Andrew Redfern, Melissa J. Davis, Erik W. Thompson
Summary: The epithelial-mesenchymal (E/M) hybrid state plays a significant role in cancer progression, facilitated by epithelial mesenchymal plasticity (EMP). Modeling and validation studies have shown the stabilised E/M hybrid states across the spectrum of EMP, along with computational approaches for characterising EMP phenotypes.
CELLS TISSUES ORGANS
(2022)
Article
Biophysics
Biswajoy Ghosh, Mousumi Mandal, Pabitra Mitra, Jyotirmoy Chatterjee
Summary: Scars are a predominant outcome of adult mammalian wound healing and may lead to partial loss of function, but studies have found that scars have stress-adaptive features that can cushion underlying tissues from external mechanical impacts.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2021)
Article
Cell Biology
Ritam Chatterjee, Biswajoy Ghosh, Mousumi Mandal, Debaleena Nawn, Satarupa Banerjee, Mousumi Pal, Ranjan Rashmi Paul, Swarnabindu Banerjee, Jyotirmoy Chatterjee
Summary: Oral sub-mucous fibrosis (OSF) is closely related to the development of oral squamous cell carcinoma (OSCC), involving oxidative stress, epithelial-mesenchymal transition (EMT), metastasis, and stemness acquisition. The study revealed that oxidative stress is associated with type-II EMT in OSF and type-III EMT in OSCC, contributing to fibrosis in OSF and malignancy-associated metastasis in OSCC.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemical Research Methods
Biswajoy Ghosh, Mousumi Mandal, Pabitra Mitra, Jyotirmoy Chatterjee
Summary: By utilizing single-scatter attenuation models, researchers found that optical coherence tomography (OCT) can correct intensity loss in images, thereby enhancing the monitoring capability of wound healing processes. They observed rapid and steady healing phases, and detected two compartments in normal scars using corrected OCT, providing a new avenue for evaluating therapeutic efficacy and scar characteristics.
JOURNAL OF BIOPHOTONICS
(2021)
Letter
Oncology
Biswajoy Ghosh, Avishek Bhandari, Mousumi Mandal, Ranjan Rashmi Paul, Mousumi Pal, Pabitra Mitra, Jyotirmoy Chatterjee
Article
Multidisciplinary Sciences
Arka Bhowmik, Biswajoy Ghosh, Mousumi Pal, Ranjan Rashmi Paul, Jyotirmoy Chatterjee, Suman Chakraborty
Summary: Precise information on localized variations in blood circulation is crucial for noninvasive cancer diagnostics and therapeutic assessment. We report a portable noninvasive blood perfusion imager augmented with machine learning-based quantitative analytics, which effectively screens precancerous and cancerous traits in oral lesions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Organic
Kumares Sarkar, Tanmaya Pathak
Summary: Terminal vinyl sulfones derived from styrene epoxide and monomesylated glycerol were reacted with benzylazide in a metal-free condition to regioselectively obtain 1,4-disubstituted 1,2,3-triazoles via Eliminative Azide-Olefinic Cycloaddition (EAOC) reactions. The use of a N, N-dimethyl ethanolammonium formate-water mixture significantly reduced reaction time and increased yield. Furthermore, the reaction of azido sugars with terminal vinyl sulfone modified carbohydrates yielded 1,4-disubstituted 1,2,3-triazole linked bissacharides, providing a potential route for direct connection of carbon units to sugar bodies.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ashrukana Das, Swagata Dasgupta, Tanmaya Pathak
Summary: This study demonstrates the design and screening of a new class of benzene-based triazolylated semicircular hybrid molecules as ribonuclease A inhibitors. The addition of a carboxylic acid group at the C1-position of the 1,3,5-trisubstituted benzene ring and the reduction of arm lengths of 3,5-substituents enhance the inhibitory properties significantly.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Pampa Mondal, Swagata Dasgupta, Tanmaya Pathak
Summary: Molecular docking studies showed that N-1,4-disubstituted-1,2,3-triazole tethered carboxymethylated thymidine and uridine can potentially bind to the P1, B1, and P2 subsites of ribonuclease A. The findings from this study, combined with previous experimental observations, were used to guide the design of a range of 1,4-disubstituted 1, 2, 3-triazole tethered carboxymethylated pyrimidine nucleosides.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Nikhil Kumar, Biswajoy Ghosh, Abhay Kumar, Riya Koley, Santanu Dhara, Santanu Chattopadhyay
Summary: In recent years, multi-stimuli responsive polymers have been proven to be highly useful as regenerative biomaterials, as they can respond to various physiological or externally applied stimuli. These biocompatible intelligent materials are designed to detect the surrounding physiological environments and release therapeutic cargos on-demand. Multi-layered smart hydrogels have been the preferred materials for drug delivery. The review highlights the formation mechanism, morphology, orientation, and formulation aspects of multi-layered structures, as well as recent studies and potential applications. The 3D bioprinting-electrospinning approach for designing smart hydrogels is also discussed as a new perspective for expanding their applications in controlled drug delivery and tissue engineering.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Ahmed Rakib, Mousumi Mandal, Anaum Showkat, Sonia Kiran, Soumi Mazumdar, Bhupesh Singla, Aman Bajwa, Santosh Kumar, Frank Park, Udai P. Singh
Summary: The polyphenolic compound piceatannol (PC) has been found to have beneficial effects on metabolic diseases, antiproliferative, antioxidant, and anti-cancer properties. This study investigated the effects of PC on inflammatory responses and adipogenesis. The results showed that PC induced apoptosis in activated T cells, reduced immune cell activation and inflammatory response, and hindered adipogenesis.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Sonia Kiran, Mousumi Mandal, Ahmed Rakib, Amandeep Bajwa, Udai P. Singh
Summary: This study investigates the role and mechanism of miR-10a-3p in adipose inflammation and adipogenesis using ex vivo and in vitro approaches. The findings suggest that miR-10a-3p mimic reduces the expression of inflammatory cells and cytokines, and induces the expression of FoxP3 in adipose tissue. In vitro experiments also show that miR-10a-3p mimic reduces the expression of inflammatory genes and lipid accumulation. This study provides a new opportunity for the development of miR-10a-3p as a novel therapeutic for adipose inflammation and its associated metabolic disorders.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biology
Biswajoy Ghosh, Krishna Agarwal
Summary: Optical microscopes have revolutionized our understanding of life by pushing the limits of speed, quality, and observable space in biological specimens. While label-based microscopy has been widely used in life science research, the use of label-free microscopy has been limited. This article discusses the integrative potential and applications of label-free optical microscopes for unperturbed analysis of biological samples.
COMMUNICATIONS BIOLOGY
(2023)