Various electron donors for biological nitrate removal: A review

Nitrate (NO3-) pollution in water and wastewater has become a serious global issue. Biological denitrification, which reduces NO3- to N-2 (nitrogen gas) by denitrifying microorganisms, is an efficient and economical process for the removal of NO3- from water and wastewater. During the denitrification process, electron donor is required to provide electrons for reduction of NO3-. A variety of electron donors, including organic and inorganic compounds, can be used for denitrification. This paper reviews the state of the art of various electron donors used for biological denitrification. Depending on the types of electron donors, denitrification can be classified into heterotrophic and autotrophic denitrification. Heterotrophic denitrification utilizes organic compounds as electron donors, including low-molecular-weight organics (e.g. acetate, methanol, glucose, benzene, methane, etc.) and high-molecular-weight organics (e.g. cellulose, polylactic acid, polycaprolactone, etc.); while autotrophic denitrification utilizes inorganic compounds as electron donors, including hydrogen (H-2), reduced sulfur compounds (e.g. sulfide, element sulfur and thiosulfate), ferrous iron (Fe2+), iron sulfides (e.g. FeS, Fe1-xS and FeS2), arsenite (As(III)) and manganese (Mn(II)). The biological denitrification processes and the representative denitrifying microorganisms are summarized based on different electron donors, and their denitrification performance, operating costs and environmental impacts are compared and discussed. The pilot- or full-scale applications were summarized. The concluding remarks and future prospects were provided. The biodegradable polymers mediated heterotrophic denitrification, as well as H-2 and sulfurmediated autotrophic denitrification are promising denitrification processes for NO3- removal from various types of water and wastewater. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

This paper discusses the issue of nitrate pollution in water and wastewater, as well as the use of various electron donors for biological denitrification. Depending on the types of electron donors, denitrification can be classified into heterotrophic and autotrophic denitrification.