Article
Biochemistry & Molecular Biology
Yun Wang, Hongbin Zou, Juan Lai, Ze Zhang, Wei Sun
Summary: The study revealed that miR-282-5p coordinates the regulation of chitinase 5 in the moulting process of silkworms, and the 20E signaling pathway plays a crucial role in normal moulting.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Physiology
Min-Li Dai, Wen-Tao Ye, Xue-Jian Jiang, Piao Feng, Qing-Yu Zhu, Hai-Na Sun, Fan-Chi Li, Jing Wei, Bing Li
Summary: This study investigated the effects of the generalist parasitoid Exorista japonica on the biological traits of its lepidopteran host, Bombyx mori. It was found that parasitism by E. japonica shortened the molting duration and decreased the emergence rate of the host, while also affecting the hormone levels and gene expression related to molting and pupation. These findings highlight a novel target mechanism employed by tachinid parasitoids and provide new insights into host/parasitoid relationships.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Entomology
Ruonan Zhang, Xue Chen, Yuan Wang, Xu Bai, Qiong Yang, Yangjin Zhong, Xiao-Qiang Yu, Fengliang Jin, Wanying Yang
Summary: 20E is involved in larval molting, metamorphosis, and innate immune response in insects. It regulates AMP genes through the IMD pathway in Drosophila, but its involvement in other immune pathways is unknown. This study shows that BmMD-2A is regulated by 20E and activates downstream immune effectors to combat bacterial infections during larval metamorphosis in Bombyx mori.
Article
Biochemistry & Molecular Biology
Guoli Li, Huangli Lan, Qingyu Lu, Chunhui He, Yuting Wei, Danmei Mo, Dacai Qu, Kaizun Xu
Summary: In this study, it was found that trace pyriproxyfen could impact the metamorphosis and silk protein synthesis in silkworms through the Met2-mediated pathway. The accumulation of pyriproxyfen in the posterior silk gland caused serious damage to the development of silk glands and cocoon-forming function.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaoran Qian, Kai Chen, Lijuan Chen, Hongsheng Song, Zhongjie Zhang
Summary: This study identified the ps homologue gene in B. mori and found that PS plays an essential role in maintaining the dynamic balance of apoptosis and proliferation during metamorphosis. Loss-of-function analysis of Bmps using the binary transgenic CRISPR/cas9 system revealed delayed developmental time and abnormal appendages in Bmps-depleted animals, along with affected apoptosis and proliferation related pathways and the Hippo pathway.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Physiology
Jinxin Wu, Guanwang Shen, Die Liu, Haoran Xu, Mengyao Jiao, Yungui Zhang, Ying Lin, Ping Zhao
Summary: This study identified the upstream region of Bombyx mori ERR (BmERR) as having promoter activity and found that 20-hydroxyecdysone up-regulated its transcriptional activity. Several putative ecdysone response elements were predicted, and an EcRE-like sequence was identified. The study also revealed the interference of BmERR and BmEcR mRNA affected the expression of BmEcR and BmUSP in embryonic cells.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tomohiro Inui, Hideki Sezutsu, Takaaki Daimon
Summary: The let-7 microRNA controls the timing of developmental programs and triggers the juvenile-adult transition in various organisms. In Bombyx mori, let-7 is required for the coordination of ecdysone and juvenile hormone biosynthesis during metamorphosis.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Wenli Zhao, Bo Zhang, Zichen Geng, Yanpeng Chang, Jizhen Wei, Shiheng An
Summary: This study investigated the function and molecular mechanism of GAPDH in larval metamorphosis in Helicoverpa armigera and Spodoptera frugiperda. The results showed that GAPDH controls glucose content to ensure glucose metabolism and metamorphosis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yiling Zhang, Mingzhen Ma, Haoran Huang, Yu Zhang, Guodong Zhao
Summary: This study examined the molecular basis of gene regulation by 20-hydroxyecdysone (20E) in the silk gland of the silkworm. The analysis revealed a large number of differentially expressed genes (DEGs) that were upregulated or downregulated at different time points after 20E treatment. These findings highlight the important role of 20E in gene regulation in the silk gland of the silkworm.
Article
Entomology
Hao Sun, Jingya Chen, Ruolin Wang, Dan Liu, Na Zhang, Tong Zhang, Ling Jia, Sanyuan Ma, Qingyou Xia
Summary: This study used a genome-wide CRISPR/Cas9-based library and large-scale screening to analyze the key genes involved in the silkworm 20E signaling pathway and their mechanisms of action. Functional annotation revealed that 20E regulates key proteins in processes mainly occurring in the cytoplasm and nucleus. Pathway enrichment analysis showed that 20E can activate phosphorylation, may affect innate immunity, interfere with intracellular nutrition and energy metabolism, and ultimately lead to cell apoptosis. These findings provide a panoramic overview of the silkworm's response to 20E signaling, highlighting the utility of genome-wide CRISPR mutant libraries in deciphering hormone signaling pathways and metamorphosis regulation in insects.
Article
Biochemistry & Molecular Biology
Tai-Hang Liu, Yi Wei, Xiao-Long Dong, Peng Chen, Ling Wang, Xi Yang, Cheng Lu, Min-Hui Pan
Summary: The matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play crucial roles in innate immunity and metamorphosis in silkworms. High-activity MMPs and low-activity TIMP may enhance infection by B. mori nucleopolyhedrovirus, affecting silkworm development and causing tissue dysplasia. These findings provide insights into the conserved functions of MMPs and TIMP in human organogenesis and immunoregulation.
Article
Agriculture, Multidisciplinary
Feifei Ren, Jiming Yan, Xiong Wang, Yukai Xie, Nan Guo, Luc Swevers, Jingchen Sun
Summary: In this study, RNA-seq analysis of the silkworm midgut after BmCPV1 infection identified 2520 differentially expressed genes (DEGs). Gene Ontology (GO) functional annotation showed that the DEGs predominantly functioned in binding, cell, and cellular processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation revealed that the DEGs were mainly distributed in global and overview metabolism maps, translation, and signal transduction. Experimental validation confirmed that BmPGRP-S5 acts as a proviral host factor for BmCPV1 infection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Ryoma Tsuchiya, Aino Kaneshima, Masakazu Kobayashi, Maki Yamazaki, Yoko Takasu, Hideki Sezutsu, Yoshiaki Tanaka, Akira Mizoguchi, Kunihiro Shiomi
Summary: Diapause is a major developmental switch in insects evolved as a survival strategy under unfavorable environmental conditions. In the silkworm Bombyx mori, diapause is transgenerationally induced as a maternal effect, with progeny diapause determined by the mother's embryonic environmental temperature. The molecular mechanisms of diapause induction involve a hierarchical pathway regulated by gamma-aminobutyric acid (GABA)ergic and corazonin signaling systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Genetics & Heredity
Guang-Hua Luo, Xi-En Chen, Yao-Yu Jiao, Guan-Heng Zhu, Ru Zhang, Ramesh Kumar Dhandapani, Ji-Chao Fang, Subba Reddy Palli
Summary: This study reveals that the SoxC gene plays a crucial role in the regulation of insect ecdysis, and is associated with the expression of neuropeptide genes such as EH.
FRONTIERS IN GENETICS
(2022)
Article
Immunology
Mengmeng Zhang, Shigang Fei, Junming Xia, Yeyuan Wang, Hongyun Wu, Xian Li, Yiyao Guo, Luc Swevers, Jingchen Sun, Min Feng
Summary: This study is the first to investigate the role of Sirtuins in insect-virus interactions. It found that silkworm Sirt2 and Sirt5 have antiviral functions against BmNPV, and silkworm Sirt5 enhances host antiviral response by activating the immune pathway mediated by Relish.
FRONTIERS IN IMMUNOLOGY
(2022)
