Article
Entomology
Jing Li, Shuai Li, Jing Li, Xinyang Tan, Zhichang Zhao, Lei Jiang, Ary A. Hoffmann, Jichao Fang, Rui Ji
Summary: This study reveals that the egg-associated secretions (EAS) from brown planthoppers (BPH) significantly affect the immunity of rice and enhance plant defenses in both rice and tobacco. The proteome analysis of EAS identifies several proteins that may have evolutionary conservation of effector functions across feeding and oviposition, providing valuable information for effector studies.
Article
Biochemistry & Molecular Biology
Yu-Cheng Xie, Hou-Hong Zhang, Han-Jing Li, Xiao-Ya Zhang, Xu-Mei Luo, Ming-Xing Jiang, Chuan-Xi Zhang
Summary: Digestion and absorption of old cuticles during insect molting are essential for new cuticle formation. This study identified 33 trypsin and trypsin-like homologs, 14 metallocarboxypeptidase and 32 aminopeptidase genes in the brown planthopper. Gene silencing experiments showed that certain trypsin-like proteases and a metallocarboxypeptidase were essential for ecdysis in the brown planthopper. These proteins exhibited specific expression patterns and were predominantly located in the exuvial space.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Physiology
Hui-Ru Si, Si-Si Sun, Yong-Kang Liu, Ling-Yu Qiu, Bin Tang, Fang Liu, Qiang Fu, Cai-Di Xu, Pin-Jun Wan
Summary: In this study, the knockdown of NlGFAT or NlPFK in brown planthoppers resulted in metabolism disorders, with significant changes in trehalose, glucose, and glycogen metabolism pathways. Knockdown of NlGFAT led to increased trehalose and glycogen content, while knockdown of NlPFK caused extreme increases in glucose and glycogen content. The knockdown of NlGFAT or NlPFK also affected the glycolytic pathway and pyruvate kinase activity, as well as ATP content.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Environmental Sciences
Gu Gong, Yingying Hong, Xuemei Wang, Surajit De Mandal, Junaid Zafar, Ling Huang, Fengliang Jin, Xiaoxia Xu
Summary: Bacterial symbionts in insect hosts have important functions in nutrient synthesis, metabolism, development, reproduction, and immunity. A study on brown planthoppers found that gut symbionts, such as Delftia NLG11, play a role in detoxifying insecticides and improving host adaptation to unfavorable environments. The cooperation between Delftia NLG11 and CYP6AY1 was observed, and it was suggested that insects can adapt to hostile environments by interacting with their symbionts, providing new insights for integrated pest management strategies.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Ni Wang, Chao Zhang, Min Chen, Zheyi Shi, Ying Zhou, Xiaoxiao Shi, Wenwu Zhou, Zengrong Zhu
Summary: In this study, miRNAs in short-winged female adults of the brown planthopper were identified using Solexa sequencing. A total of 905 miRNAs were identified, and 43 miRNAs showed differential expression in different developmental phases. These differentially expressed miRNAs may play important roles in the reproductive development of the brown planthopper.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Jun Hu, He Cui, Mingsheng Hong, Yuxian Xia, Wenqing Zhang
Summary: This study constructed transgenic strains of Metarhizium anisopliae and demonstrated that the expression of dsRNA targeting the chitin synthase gene greatly enhanced the fungal virulence against the brown planthopper Nilaparvata lugens.
Article
Biochemistry & Molecular Biology
Lu Zhang, Xu Cheng, Shuai Tao, Lu-Yao Peng, Zhen Zhu, Yan-Yuan Bao
Summary: In this study, a novel function of the NCS2 protein as a regulator in development and reproduction was revealed in the hemipteran Nilaparvata lugens. Knockdown of NCS2 in nymphs led to lethality and disrupted lipid droplet arrangement in the ovaries and testes, highlighting its importance in modulating lipid droplet remodelling.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ni Wang, Min Chen, Ying Zhou, Wen-Wu Zhou, Zeng-Rong Zhu
Summary: This study systematically characterized five core genes involved in miRNA biogenesis and found that they play essential roles in the development and reproduction of the Nilaparvata lugens agricultural pest.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Entomology
Jun Yang, Xiang-Dong Kong, Keyan Zhu-Salzman, Qing-Ming Qin, Qing-Nian Cai
Summary: This study investigates how brown planthopper uses glutathione S-transferases to detoxify gramine in rice, showing that gramine induces the activity and gene transcription of glutathione S-transferase in brown planthopper. Knockdown of key genes NlGST1-1, NlGSTD2, and NlGSTE1 inhibits the enzyme's activity, providing a potential pest control strategy.
Article
Biochemistry & Molecular Biology
Ding-Bang Hu, Shuai Xiao, Yefu Wang, Hong-Xia Hua
Summary: This study reveals that Notch in the brown planthopper is a complex alternatively spliced gene that codes for a series of proteins. It plays a role in the development of sensation circles, bristles, veins, and wings in the late nymph stage.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Physiology
Xiao-Xiao Shi, Mu-Fei Zhu, Ni Wang, Yuan-Jie Huang, Min-Jing Zhang, Chao Zhang, Soomro A. Ali, Wen-Wu Zhou, Chuanxi Zhang, Cungui Mao, Zeng-Rong Zhu
Summary: The study demonstrates the pivotal role of neutral ceramidase (NCER) in female reproduction of the brown planthopper, influencing oocyte maturation, oviposition, and egg hatching rate through regulation of ceramide levels.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ayushi Gupta, Suresh Nair
Summary: DNA methylation plays a critical role in cellular differentiation, development, gene regulation, genome integrity, and phenotypic plasticity in insects. However, its evolutionary potential and role in facilitating rapid adaptations in insects remain unclear. In this study, methylation patterns in the brown planthopper (BPH), a major rice pest, were investigated under pesticide and nutritional stress across its life stages. The heritability and persistence of stress-induced methylation marks in BPH were also determined. The results showed that DNA methylation patterns in BPH vary with environmental cues and are specific to each life stage. Additionally, it was found that the stress-induced methylation marks in BPH are heritable but fade over generations in the absence of stressors, suggesting potential fitness costs associated with maintaining the stressed epigenotype. Furthermore, the study demonstrated the impact of 5-azacytidine-mediated disruption of BPH methylome on the expression of stress-responsive genes, highlighting demethylation/methylation as a phenomenon underlying stress resilience in BPH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Min Zhou, Qida Shen, Shasha Wang, Guoyong Li, Yan Wu, Caidi Xu, Bin Tang, Can Li
Summary: This study investigated the regulation of trehalose-6-phosphate synthase (TPS) in chitin metabolism in the brown planthopper. The results showed that inhibition of TPS expression affected the metabolism of trehalose and chitin in the insect. These findings provide a theoretical basis for pest control.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiao-Xiao Shi, He Zhang, Md Khairul Quais, Ming Chen, Ni Wang, Chao Zhang, Cungui Mao, Zeng-Rong Zhu
Summary: In this study, the cloning and functional characterization of four sphingomyelinase homologue genes from brown planthopper (BPH) were reported. Knocking down one of the genes resulted in ovarian malformation in BPH and disrupted diacylglycerol metabolism and vitellogenin accumulation, revealing the vital role of NlSMases in BPH reproductive development.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qin Su, Jun Lv, Wan-Xue Li, Jia-Wei Sun, Shi-Hui Li, Wen-Qing Zhang
Summary: Sexually mature female brown planthoppers (BPHs) emit abdominal vibration (AV) signals for mating preparation, with the regulatory mechanism remaining unclear. Interfering with juvenile hormone binding protein (JHBP) greatly reduces the likelihood and frequency of AV emission in female BPHs.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Y. Guo, A. A. Hoffmann, X. -Q. Xu, X. Zhang, H. -J. Huang, J. -F. Ju, J. -T. Gong, X. -Y. Hong
INSECT MOLECULAR BIOLOGY
(2018)
Article
Entomology
Hai-Jian Huang, Tong-Qiang Zhang, Qiao Li, Chuan-Xi Zhang, Bao-Qin Zhang
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
(2018)
Article
Biochemical Research Methods
Hai-Jian Huang, Jia-Rong Cui, Lei Chen, Yu-Xi Zhu, Xiao-Yue Hong
Review
Biochemistry & Molecular Biology
Hai-Jian Huang, Chuan-Xi Zhang, Xiao-Yue Hong
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2019)
Article
Plant Sciences
Hai-Jian Huang, Jia-Rong Cui, Xue Xia, Jie Chen, Yu-Xuan Ye, Chuan-Xi Zhang, Xiao-Yue Hong
Article
Biochemistry & Molecular Biology
X. -T. Yan, Z. -X. Ye, X. Wang, C. -X. Zhang, J. -P. Chen, J. -M. Li, H. -J. Huang
Summary: The study found that small brown planthopper has the best adaptation on wheat plants, followed by white-backed planthopper, while brown planthopper performs the worst. The transcriptomic responses of white-backed planthopper to rice and wheat plants show significant differences. There is varied bacterial diversity among the three planthoppers, but no dramatic change in response to different host plants.
INSECT MOLECULAR BIOLOGY
(2021)
Article
Biology
Hai-Jian Huang, Xiao-Tian Yan, Zhong-Yan Wei, Yi-Zhe Wang, Jian-Ping Chen, Jun-Min Li, Zong-Tao Sun, Chuan-Xi Zhang
Summary: The bean bug, Riptortus pedestris, is a notorious pest of soybean crops in Asia, secreting a mixture of salivary proteins that induce cell death and plant defense responses. A total of 136 salivary proteins were identified in this study using transcriptomic and proteomic approaches, shedding light on the molecular mechanisms underlying plant-insect interactions. This research provides valuable insights for future studies on bug salivary effectors and pest management.
Article
Ecology
Hong-Yuan Wei, Yu-Xuan Ye, Hai-Jian Huang, Ming-Shun Chen, Zi-Xiang Yang, Xiao-Ming Chen, Chuan-Xi Zhang
Summary: The study generated a high-quality genome assembly of the aphid Schlechtendalia chinensis, providing a solid genetic foundation for understanding gall formation and the interaction between aphids and their host plants.
ECOLOGY AND EVOLUTION
(2022)
Article
Entomology
Li-Li Li, Zhuang-Xin Ye, Jian-Ping Chen, Chuan-Xi Zhang, Hai-Jian Huang, Jun-Min Li
Summary: This study revealed the full genomes of two insect-specific viruses (ISVs) in green leafhoppers and investigated the host antiviral RNA interference (RNAi) response. The findings provide valuable information on ISVs in leafhoppers and may have practical applications in pest management.
Article
Virology
Yi-Zhe Wang, Zhuang-Xin Ye, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li, Hai-Jian Huang
Summary: A novel iflavirus named Lycorma delicatula iflavirus 1 (LDIV1) was identified in the spotted lanternfly. LDIV1 replicates in the host insect and activates host antiviral immunity. Phylogenetic analysis showed that LDIV1 is a new species in the genus Iflavirus.
ARCHIVES OF VIROLOGY
(2022)
Article
Entomology
Yue Huang, Biao Hu, Zhongyan Wei, Shiqi Shan, Chunyun Guo, Hehong Zhang, Yanjun Li, Jianping Chen, Xue Kang, Haijian Huang, Zongtao Sun
Summary: R. pedestris is a major pest in soybeans causing staygreen syndrome, and recent research suggests that its salivary proteins may play a crucial role in insect infestation. This study identified Rp2155 as a salivary effector protein that induces cell death and is specifically expressed in the salivary gland of R. pedestris. It was found that silencing Rp2155 can alleviate soybean staygreen symptoms caused by R. pedestris, suggesting that it could be a potential target for insect control.
Article
Agronomy
Hai-Bin Lu, Jia-Bao Lu, Li-Li Li, Ze-Long Zhang, Jian-Ping Chen, Jun-Min Li, Chuan-Xi Zhang, Hai-Jian Huang
Summary: This study identifies 69 neutral lipases in the bean bug, R. pedestris, and analyzes their functions. The results show that several of these lipases play crucial roles in insect reproduction and survival, suggesting their potential as targets for controlling this pest.
PEST MANAGEMENT SCIENCE
(2023)
Article
Genetics & Heredity
Xin Wang, Zhuang-Xin Ye, Yi-Zhe Wang, Xiao-Jing Wang, Jian-Ping Chen, Hai-Jian Huang
Summary: This study investigates the gene expression patterns of tobacco in response to whitefly infestation. It finds that whitefly infestation activates plant defense mechanisms, allowing the plant to recognize pathogens and enhance immunity. However, it also suppresses energy metabolism, carbohydrate metabolism, ribosomes, and photosynthesis, leading to impaired plant development.
Article
Entomology
Ze-Long Zhang, Xiao-Jing Wang, Hai-Bin Lu, Hai-Jian Huang
Summary: This study investigated the gut transcriptomic changes of Riptortus pedestris when feeding on different parts of soybean plants, revealing the mechanisms by which they adapt to different food resources.
Article
Multidisciplinary Sciences
Hai-Jian Huang, Yi-Yuan Li, Zhuang-Xin Ye, Li-Li Li, Qing-Ling Hu, Yu-Juan He, Yu-Hua Qi, Yan Zhang, Ting Li, Gang Lu, Qian-Zhuo Mao, Ji-Chong Zhuo, Jia-Bao Lu, Zhong-Tian Xu, Zong-Tao Sun, Fei Yan, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li
Summary: Non-retroviral endogenous viral elements (nrEVEs) are widely dispersed in eukaryotic genomes, with their functions largely unknown. In this study, researchers discovered that a ToEVE in planthoppers has been domesticated and plays essential roles in their development and reproduction. Analysis of arthropod genomes suggests that nrEVEs may contribute to the functional diversity of arthropod genes.
NATURE COMMUNICATIONS
(2023)